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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2019Quote: ... followed by qPCR analysis on a 7500 Real-Time PCR System with the Power SYBR® Green RNA-to-CT™ 1-Step kit (Applied Biosystems, USA) using primers listed in Table 1 ...
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bioRxiv - Biophysics 2022Quote: ... was reverse-transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosciences, Carlsbad CA, cat #) followed by real-time quantitative PCR (qPCR) reactions using SYBR Green (Life Technologies, Carlsbad, CA, cat #) 73 ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cDNA was run for quantitative PCR using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) kit on QuantStudio 6 and 7 Flex real-time PCR systems (Thermo Fisher, USA). Reagents mentioned above were obtained from Takara (Japan) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was performed using a real-time system and SYBR green kit (Applied Biosystems). Relative gene expression was compared to actin as an internal control ...
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bioRxiv - Molecular Biology 2020Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described97 ...
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bioRxiv - Cell Biology 2020Quote: ... Gene expression was measured using real-time quantitative PCR (qRT-PCR) with TaqMan™ Master Mix II no UNG (Cat 4440049, Thermofisher Scientific) with the following primer/probe sets ...
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bioRxiv - Cell Biology 2019Quote: Quantitative RT-PCRs for CAP-D2 and CAP-D3 transcripts were done in triplicates and from three independent biological samples using SYBR™ Green PCR Master Mix (Thermo Fischer Scientific) in a 7900HT Fast Real-Time PCR System (Applied Biosystems). For each reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... All reactions were performed by a 2-step PCR in triplicates followed by melting curve analysis on a ViiA7 real-time PCR cycler (Thermo Fisher Scientific). Primers are specified in Supplementary table 2 ...
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bioRxiv - Pathology 2022Quote: ... Real-time PCR reactions were performed using a QuantStudioTM 12K Flex Real-Time PCR System with TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays (probes, Thermo Fisher Scientific). Following probes were used ...
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bioRxiv - Immunology 2022Quote: ... cDNA was diluted 1:5 in water and 2 μl was used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and QuantiStudio 5 Real-Time PCR machine (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: DNA or cDNAs were subjected to quantitative PCR (qPCR) using KOD SYBR qPCR Mix (TOYOBO) in the StepOnePlus Real-time PCR system (Thermo Fisher Scientific) (1 µL of DNA or cDNA per 10 µL reaction in duplicate) ...
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bioRxiv - Developmental Biology 2019Quote: ... Residual plasmids used for iPSC establishment were analyzed by TaqMan™ quantitative PCR using StepOnePlus™ Real-Time PCR Systems (Thermo Fisher). Primer and probe sequences (cmCAG ...
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... qRT-PCR and data collection were carried out on an ABI 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA). The relative expression was calculated and normalized using the 2−ΔΔCt method relative to 18S and/or GUSB.
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed with a 1:2 dilution of cDNA by using the StepOnePlus Real-Time PCR-System (Thermo Fisher Scientific) or the CFX384 Touch™ Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was diluted 1:5 in water and used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) in a LightCycler® 96 (Roche) ...
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bioRxiv - Immunology 2021Quote: ... mRNA expression levels were analyzed using SYBR Green PCR Master Mix and a QuantStudio 12K Flex Real-Time PCR System (both from Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1 ...
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR (qPCR) was performed using a QuantStudio 3 Real-Time PCR System in conjunction with a SybrGreen System (Thermo Fisher Scientific) in conjunction with a SybrGreen System for the expression of Atf4 ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR reactions were performed on a StepOne Real Time PCR System using TaqMan or SYBR Green Universal PCR Master Mix (Applied Biosystems, USA) and Rn18s (18S rRNA ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed using QuantStudio™ 6 Flex real-time PCR system with power SYBR® green PCR master mix (Applied Biosystems). All gene expression data were normalized by GAPDH using the comparative CT method (2-ΔΔCt) ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was prepared using 1 μg of DNase treated RNA followed by q-PCR using StepOnePlus Real-Time PCR system (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 in water and 2 μl was used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a 7900HT Real-Time PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described (23) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative reverse transcription PCR was performed with the SYBR master mix and StepOne real-time PCR machine (Applied Biosystems, Waltham, MA, USA). The thermal cycling program was as follows ...
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bioRxiv - Plant Biology 2019Quote: ... The qRT-PCR reactions were performed on an ABI Prism 7500 Fast real-time PCR System (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Microbiology 2019Quote: ... cDNA was diluted 1:5 in water and 2 µl was used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a Quant Studio 5 real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... 25 ng of cDNA was subjected to quantitative PCR (qPCR) on a Step One Plus™ Real-Time PCR System (Thermo Fisher) using the TaqMan Fast Universal PCR Master Mix (Thermo Fisher # 4364103) ...
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bioRxiv - Cancer Biology 2022Quote: ... Relative quantitation of gene expression was determined by real-time PCR which was carried out using SYBR® Green PCR Master Mix (Applied Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: cDNA synthesis was performed using SuperScript VILO and quantitative PCR performed using TaqMan probes and reagents with the StepOne Plus Real-Time PCR System (Thermo Fisher Scientific) with 50ng of product per reaction ...
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bioRxiv - Physiology 2022Quote: Quantitative PCR (qPCR) (Aw et al., 2018) was performed using a ABI 7300 Real Time PCR System (Applied Biosystems, Waltham, MA, USA) and Power SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2022Quote: ... as an internal control in Applied Biosystems 7500 Real Time PCR System with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... PCR was run using THUNDERBIRD SYBR qPCR Mix (TOYOBO, Osaka, Japan) on QuantStudi 12K Flex Real-Time PCR System (Thermo Fisher Scientific). The primers for transgene expression were designed using the terminator sequence of pCAMBIA1390 (Table S2) ...
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bioRxiv - Cancer Biology 2022Quote: ... This single-stranded cDNA was then used to perform quantitative real-time PCR (qRT-PCR) with specific primers using PowerUP SYBR Green 2x Master Mix (Thermo Fisher Scientific) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... PCRs were carried out using the standard setting in a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific, Waltham, MA, U.S.A.). The mean cycle threshold (Ct ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time quantitative reverse transcription-PCR (qRT-PCR) analyses were carried out using a PowerUp SYBR Green Master Mix (Applied Biosystems, USA) on QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative-PCR (qPCR) was performed with Power SYBR Green on the 7500 Fast Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). Chromatin immunoprecipitation was performed as previously described (Garcia-Gutierrez et al ...
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bioRxiv - Neuroscience 2020Quote: ... The presence of the SARS-CoV-2 in these samples was evaluated by one-step qRT-PCR in a final volume of 25 μL per reaction in 96-well PCR plates using a thermocycler (7500t Real-time PCR system, Applied Biosystems, Applied Biosystems). Briefly ...
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bioRxiv - Immunology 2021Quote: ... cDNA was diluted to 100 ng/μl and quantitative PCR (qPCR) was performed using TaqMan Real-Time PCR Assays for designated genes and TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... One μl of the reaction products was then used for Q-PCR in an Applied Biosystem™ 7500 Fast Real-Time PCR system (ThermoFisher Scientific) with gene-specific primers (Table S3).
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bioRxiv - Microbiology 2020Quote: DNA or cDNAs were subjected to quantitative PCR (qPCR) using KOD SYBR qPCR Mix (TOYOBO) in the StepOnePlus Real-time PCR system (Thermo Fisher Scientific) (1 μL of DNA or cDNA per 10 μL reaction in duplicate) ...
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bioRxiv - Immunology 2021Quote: cDNA was quantified by real-time PCR using the SYBR green method with 2x SYBR Green PCR Master Mix (Thermo Fisher Scientific) and a StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using qPCRBIO SyGreen Blue Mix (PCR Biosystems) and run on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). Primer sequences used were predesigned KiCqStart SYBR® Green primers (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). TaqMan© assays were used to quantitate ERRα (Hs00607062_gH) ...
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bioRxiv - Biochemistry 2022Quote: ... Quantitative real-time PCR was performed using the Power SYBR Green PCR Master Mix and a Step-one Plus Real-Time PCR system (Thermo Fisher Scientific) to assess copy numbers for the mitochondrial gene cytochrome b (cytB ...
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bioRxiv - Cell Biology 2022Quote: ... DNA prepared from the whole-cell extracts or immunoprecipitated fractions was analyzed via quantitative PCR (qPCR) with the StepOnePlus Real-Time PCR system (Thermo Fisher Scientific) using Power SYBR Green PCR Master Mix© Green I Master (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: Quantification using real-time PCR was initiated using 10 ng of cDNA with SYBR Green PCR Master Mix (2×) (Applied Biosystems, 4364346) in a LightCycler II® 480 (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was performed with specific primers (Supplemental Table S4) using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems, Foster City, CA, USA) in a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Ten nanograms of cDNA were used for the real time PCR using ABI SyBr Green PCR Master mix (Applied Biosystems, Life Technologies) in QuantStudio 12K Flex Real-Time PCR System (Life Technologies ...