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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared and Real-time PCR was performed by using FastSYBR Green Master Mix (Applied Biosystems) in Via7 Real-Time PCR Instrument (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Target gene expressions were measured using real-time PCR using Maxima SYBRGreen MasterMix (Applied Biosystems, Waltham, USA) with an ABI Prism 7500 instrument (Applied Biosystems) ...
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed in 386 wells plates in QuantStudio™ Real-Time PCR System (Applied Biosystems™) using the Taqman® Gene Expression Assay kit following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The real-time PCR measurement of cDNAs was performed using PowerUp SYBR Green Master Mix (Applied Biosystems) and normalized to the expression of myoglobin as standard for the housekeeping gene ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using a QuantStudio 6 Pro Real-Time PCR machine (Thermo Fisher Scientific, Waltham, MA). The following primers (5’-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... was used and the reaction was carried out in a StepOnePlus Real-Time PCR System (Applied Biosystems). The β-actin and 18s genes were used as internal controls ...
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bioRxiv - Zoology 2023Quote: ... All data were analyzed by the ΔΔct method using Real-Time PCR System Software (Thermo Fisher Scientific). Transcription elongation factor SPT4 or the α-tubulin gene of C ...
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bioRxiv - Zoology 2023Quote: ... All reactions were performed using the Real time PCR system STEP1 (Thermo Fisher Scientific K.K., Tokyo, Japan) with Power up SYBER (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Real time qPCR was performed in triplicate using Power SYBR™ Green PCR Master Mix (Applied Biosystems) on the StepOnePlus RealTime PCR System (Applied Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... The melt curve step was carried out in a QuantStudio 6 Real-Time PCR system (Applied Biosystems). The PCR process involved UDG (Uracil-DNA Glycosylase ...
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bioRxiv - Immunology 2023Quote: ... cDNAs were used for Real time PCR using PowerUP Sybr Green Master Mix (Applied Biosystems, cat. 100029283) and a Lightcycler LC480 instrument (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Amplification was carried out in a QuantStudio™ 6 Flex Real-Time PCR System (#4485691, Applied Biosystems) under the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... The qPCR amplification reaction was performed in a 7500 Fast Real-Time PCR system from Applied Biosystems. A 20 µl reaction mixture contained Power SYBR® Green PCR master mix (Ref# 4367659 ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR analysis was conducted on a QuantStudio 6 Flex Real-Time PCR System (4485697; Thermo Fisher Scientific) with Luna Universal qPCR Master Mix (M3003X ...
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bioRxiv - Microbiology 2023Quote: ... using a QuantStudio 7 Flex Real-Time PCR System utilizing TaqMan Gene Expression Master Mix (Applied Biosystems).
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bioRxiv - Neuroscience 2024Quote: ... according to the manufacturer’s protocol with a QuantStudio 12 K Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Microbiology 2024Quote: ... The cycling conditions were set in the PikoReal™ Real-Time PCR System (Thermofisher Scientific, Waltham, MA) as follows ...
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bioRxiv - Bioengineering 2024Quote: ... Quantitative real-time PCR (qPCR) was performed using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific), a pre-formulated universal 2X master mix containing Dual-Lock Taq DNA polymerase for real-time PCR ...
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bioRxiv - Plant Biology 2024Quote: ... Transcript levels were assessed using a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA). In the presence of 10-µL KAPA SYBR FAST qPCR Kit (Nippon Genetics Co ...
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bioRxiv - Biochemistry 2024Quote: Thermal shift assays were performed with a StepOnePlus real-time polymerase chain reaction (PCR) machine (Life Technologies) using SYPRO Orange dye (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... using an Applied Biosystems™ QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific, cat# 4471087) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was amplified using the 7300 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Darmstadt, Germany) with the following program for the SYBRGreen method ...
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bioRxiv - Cancer Biology 2024Quote: ... The amplified cDNA library was quantified using the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher) through qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... The details of the real-time-qPCR reaction using Power SYBR Green PCR Master Mix (Applied Biosystems) in a Quant Studio 12K Flex Real-Time PCR machine 285880228 (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... Relative gene expression was determined using a QuantStudio 12K Flex Real-Time PCR system (Thermo Fisher Scientific) with SYBR Green and relative standard curves normalized against expression of cyclophilin a (Cypa) ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR was performed using the Applied Biosystems 7500 system (Applied Biosystems, Foster City, CA). Gene expression changes were calculated by the ΔCt method ...
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bioRxiv - Physiology 2024Quote: ... in the QuantStudio™ 7 Flex Real-Time PCR System (384-well block, Applied Biosystems, Cat #4485701). 18s rRNA was used as an internal reference control ...
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bioRxiv - Pathology 2024Quote: ... qPCR assays were performed in duplicate in a StepOnePlus real-time PCR system (Applied Biosystems, Life Technologies). In 96-well plates ...
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bioRxiv - Pathology 2024Quote: ... qPCR assays were performed in duplicate in a StepOnePlus real-time PCR system (Applied Biosystems, Life Technologies). In 96-well plates ...
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bioRxiv - Cell Biology 2024Quote: ... and the corresponding TaqMan Assay in a StepOnePlus Real-Time PCR Cycler (Applied Biosystems, Waltham, Massachusetts, USA). Data were assessed using the 2-ΔΔCt method (Schmittgen and Livak ...
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bioRxiv - Genomics 2024Quote: ... Real-time quantitative PCR was performed and analyzed using Power SYBR Green Master Mix (Thermo Fisher Scientific) reagents on the QuantStudio 6 flex real time PCR system ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed using TaqMan Gene Expression solutions using a QuantStudio Real-Time PCR System (Life Technologies). RPL36 or CASC3 were used as house-keeping genes to normalize critical threshold (CT ...
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bioRxiv - Synthetic Biology 2024Quote: ... The thermal cycling and data collection were performed on Quantstudio 5 Real-Time PCR instrument (Thermo Fisher), using the thermal cycling protocol 2 min at 50 °C ...
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR was performed on cDNA using Taqman preAmp MasterMix and Taqman Universal Mastermix (ThermoFisher) and Assays-on-Demand probes (Gapdh ...
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bioRxiv - Immunology 2024Quote: ... Each sample was amplified in triplicate or duplicate in a StepOnePlus real-time PCR system (Applied Biosystems). mRNA levels were calculated with the StepOne v2.1 software (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2024Quote: ... qPCR was conducted with three biological replicates on a StepOnePlus Real-Time PCR System (Applied Biosystems, USA) using the GB-Amp™ Sybr Green qPCR Mix (GeneBio ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR products were created using the Superscript IV One Step RT-PCR system (Thermofisher) and primers designed based on the results of Sanger Sequencing (Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... an iterative process of RT-PCR (Superscript III platinum 1-step RT-PCR, Thermo Fisher) and topo cloning (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR reactions were performed using SuperScript III One-Step RT-PCR system (ThermoFisher, #12574030) following manufacturing protocol ...
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bioRxiv - Plant Biology 2019Quote: Expression studies were carried out by real-time reverse transcription polymerase chain reaction (RT-qPCR) using the StepOne plus thermocycler (Applied Biosystems), using the Power SyBR Green master mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time RT-qPCR for DNA was performed using PowerUP SYBR Green master mix (Thermo fisher scientific, Grand Island, NY, USA) and specific primers on a Stratagene Mx3005P instrument (Agilent Technology ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time RT-qPCR for mRNA was performed using PowerUP SYBR Green master mix (Thermo fisher scientific, Grand Island, NY, USA) and specific primers on a Stratagene Mx3005P instrument (Agilent Technology ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR) were performed on the 7500 Dx Fast thermal cycler (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR) were performed on the 7500 Dx Fast thermal cycler (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was performed on an Applied Biosystems StepOnePlus™ using Fast SYBR (Thermofisher). The relative change in gene expression was analyzed in triplicate using the 2-ΔΔCT method47 with β-actin as the reference gene.
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers nCoV_IP2-12669Fw ...
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bioRxiv - Microbiology 2021Quote: RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers ORF1ab_Fw ...
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bioRxiv - Neuroscience 2024Quote: ... the DNA fragment encoding miRNA for Dsp (5’-TGC TGT TGA GAT GGA CAT CAA TGC ACG TTT TGG CCA CTG ACT GAC GTG CAT TGG TCC ATC TCA A-3’ and 5’-CCT GTT GAG ATG GAC CAA TGC ACG TCA GTC AGT GGC CAA AAC GTG CAT TGA TGT CCA TCT CAA C-3’) was subcloned into pcDNA6.2-GW-EmGFP-miR using BLOCK-iT Pol II miR RNAi Expression Vector Kit (Invitrogen). The EmGFP and Dsp miRNA fragments were amplified by PCR from this vector and inserted between the KpnI and EcoRV sites of the pAAV-EF1α-DIO EYFP vector (#27056 ...
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bioRxiv - Neuroscience 2024Quote: ... Two microliters of complementary DNA was diluted 5 times and added to a 20-µL reaction volume with SYBR Green real-time PCR mixture (Thermo Fisher Scientific, #A25778). Real-time PCR was performed using the QuantStudio 6 Flex real-time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Library quantification was performed using the Collibri™ Library Quantification Kit (Thermo Fischer Scientific, A38524500) in a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific).Both batches were sequenced in a NovaSeq6000 sequencer (Illumina ...