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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed using SYBR Green PCR mastermix (Genetix Biotech Asia Pvt. Ltd) on the QuantStudio Real-Time PCR System (Thermo Fisher Scientific). Primer sequences are provided in the supplementary table 7.
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bioRxiv - Cancer Biology 2023Quote: ... β-Actin was used as reference control and qRt-PCR was run on the PikoReal Real-Time PCR System (Thermo Fisher Scientific). The relative fold change in gene expressions were measured with the 2(−ΔΔCT ...
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bioRxiv - Microbiology 2023Quote: ... qPCR analysis was performed with biological triplicates and technical duplicates using QuantStudio 5 real-time PCR System and Power SYBR Green PCR Master Mix (Thermo Fisher Bioscientific) (13 ...
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bioRxiv - Cell Biology 2023Quote: ... were added to 10 µL PCR mix and fluorescence was detected using StepOnePlus™ Real-Time PCR System (Applied Biosystems™, 4376600). Data were analyzed using High Resolution Melting (HRM ...
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bioRxiv - Cell Biology 2023Quote: ... 2 pmol forward/ reverse primer pairs (sequences listed in Table S2), and the BIO SyGreen Lo-ROX mix (PCR Biosystems, London, UK) on an ImageQuantQ3 Real-time PCR System (Thermo Fisher Scientific) in sealed 96-well optical reaction plates (Applied Biosystems) ...
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bioRxiv - Bioengineering 2023Quote: ... The titers of AAVs were calculated by real-time quantitative PCR using SYBR Green PCR Master Mix (Thermo Fisher Scientific, Waltham, MA). FUS-BBBO was performed on Ai9 mice to deliver AAV9s to the left hemisphere with 22 target sites ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR analysis was conducted on an Applied Biosystems QuantStudio™ 6 Flex Real-Time PCR System with Power SYBR Green PCR Master Mix (Applied Biosystems). Expression levels were determined with the delta Ct method and normalized to GAPDH mRNA ...
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bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:5 in water and 2 μl used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a Quant Studio 5 (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Thermal cycling and rRT-PCR analyses for all assays were performed on the ABI 7500 FAST real-time PCR system (Applied Biosystems, USA) with the following thermal cycling profile ...
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bioRxiv - Immunology 2022Quote: ... and subjected to the one-step quantitative real-time reverse transcription-PCR assay (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat# 4444432) as described previously58 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was then diluted and used as the template for real-time PCR analysis using the Power up SYBR Green PCR Master Mix (ThermoFisher Scientific, # A25779) on the QuantStudio3 Real-Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ten nanograms of cDNA were used for the real time PCR using ABI SyBr Green PCR Master mix (Applied Biosystems, Life Technologies) in QuantStudio 12K Flex Real-Time PCR System (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Ten nanograms of cDNA were used for the real time PCR using ABI SyBr Green PCR Master mix (Applied Biosystems, Life Technologies) in QuantStudio 12K Flex Real-Time PCR System (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was performed with specific primers (Supplemental Table S4) using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Cell Biology 2024Quote: ... was performed on 12 ng cDNA with SybrGreen PCR mastermix (LifeTechnologies) and specific primers (supplementary Table 2) and run in an ABI? real-time PCR machine (Applied Biosystems, ThermoFisher Scientific). The raw data was analysed through -ΔCt as described in Supplementary Methods.
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed by using the Powerup™ SYBR Green Master Mix (Thermo Fisher Scientific, Cat#A25918). TATA Binding Protein (TBP ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was performed using SYBR Green Realtime PCR Master Mix (TOYOBO) on ABI QuantStudio 6 flex Real-time PCR System (Thermo Fisher Scientific). The RT-qPCR Primer sequences for targeting respective genes are displayed in Table S1 ...
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bioRxiv - Cancer Biology 2021Quote: ... miR-122 and anti-miR-122 were purchased from Ambion and was used at 100 pmoles to transfect cells per well of a six-well plate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... miR-146a or miR scramble control (ThermoFisher Scientific, Waltham, MA) were mixed with PEI at an N:P ratio of 25 (the ratio of moles of PEI amine groups to nucleic acid phosphate groups ...
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bioRxiv - Physiology 2020Quote: ... The multiplex RT primer pool consisted of primers for miR-150-5p and U6 (Thermo Fisher Scientific). No-template samples where included as negative controls.
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bioRxiv - Microbiology 2020Quote: We performed a multiplex RT-qPCR assay using the TaqPath RT-PCR COVID-19 kit (Thermo Fisher CN A47814) together with the TaqPath 1-step master mix – No ROX (Thermo Fisher CN A28523) ...
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bioRxiv - Microbiology 2020Quote: ... The RT-qPCR reaction was prepared using the AgPath-ID-One-Step RT-PCR kit (Thermo Fisher Scientific, USA) adjusted to a volume of 12.5 μl [19] ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by RT-PCR reaction using SYBR Green PCR Master Kit in QuantStudio 5 Systems (Applied Biosystems). Gene expression was normalized to GAPDH using the comparative CT method.
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bioRxiv - Microbiology 2020Quote: ... from the swab samples was based on Cq values obtained by reverse transcriptase quantitative real-time PCR (RT-qPCR) targeting the matrix (M) gene 63 and using AgPath-ID One-Step RT-PCR reagent kit (Thermo Fisher Scientific, Waltham, MA,USA) on a a BioRad CFX1000 Real-Time PCR System (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... the isolated viral RNA was tested by one-step RT-PCR using the Verso 1-Step RT-PCR Kit (Hot Start) (Thermo Fisher Scientific, Waltham, MA, USA) and nested primers—MarBox1F and MarBox1R ...
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bioRxiv - Molecular Biology 2022Quote: ... were amplified by RT-PCR using the Verso 1-Step RT-PCR Kit with Thermo-Start Taq (Hot Start; Thermo Fisher Scientific, Waltham, MA, USA) using primers Mar- CPL-F and MarCP2R (876 bp ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was quantified by quantitative PCR on an ABI Prism 7500 real-time PCR system using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and primers amplifying a fragment of DHX37 transcript (forward 5’-CCCGATATCGAGAAAGCCTGG-3’ ...
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bioRxiv - Microbiology 2020Quote: Quantitative PCR was performed on a QuantStudio™ 12K Flex Real-Time PCR System using a Taqman™ Universal PCR Mastermix (Applied Biosystems, Grand Island, NY). For the quantification of eubacteria and archaea ...
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bioRxiv - Biochemistry 2020Quote: ... The quantitative RT-PCR reactions were performed on an Applied Biosystems 7500 Sequence Detection system (Applied Biosystems, Foster, CA, USA) using ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme BioTech Co.,Ltd.) ...
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bioRxiv - Genetics 2023Quote: ... RT–PCRs of different genes using respective primers (Table S2) were performed using ABI-7300 RT–PCR with Sequence Detection System v1.4 (Applied Biosystems). Relative mRNA expression was calculated using the previously established 2−ΔΔCT method [48] ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting cDNA was diluted 4 times with H2O and mixed with 2X TaqMan OpenArray Real Time PCR Master Mix (Life Technologies, Grand Island, NY) at a ratio of 3.8:1.2 (PCR mix:cDNA) ...
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bioRxiv - Plant Biology 2021Quote: Initial validation of the flg22 treatment was assessed with real-time (RT) quantitative polymerase chain reactions (qPCR) on a QuantStudio 6 instrument (Thermo Fisher Scientific), using the total RNA extracted for the CAGE library preparations and the ΔΔCt method ...
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bioRxiv - Immunology 2023Quote: DDA-SPS-MS3 method with online real-time database search (RTS) was performed on an UltiMate 3000 Dual LC nano-HPLC System (Dionex, Thermo Fisher Scientific), containing both a trapping column for peptide concentration (PepMap C18 ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse-transcriptase real-time quantitative polymerase chain reaction (RT-qPCR) was carried out with TaqMan® Gene Expression Assays (Thermo Fisher Scientific) in complementary deoxyribonucleic acid (cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... The TaqPathTM COVID-19 CE-IVD RT-PCR kit (ThermoFisher Scientific, Waltham, US) was used to identify the presence of SARS-CoV-2 ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR was performed using the Superscript III first-strand synthesis kit (Invitrogen) and cytochrome gene-specific primers (Table S1 ...
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bioRxiv - Developmental Biology 2020Quote: ... for RT-PCR and Arcturus PicoPure RNA isolation kit (Applied Biosystems 12204-01) for qPCR and sequencing using manual guidelines ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using SYBR-Green PCR Master Mix kit (Applied Biosystems) with an ABI7500 real-time qPCR system (ABI Company ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed using the One-Step RT-PCR kit High Fidelity (Invitrogen) in a volume of 50 μl containing 5.0 μl eluted RNA with final concentrations of 1xSuperScript III One-Step RT-PCR buffer ...
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bioRxiv - Genetics 2020Quote: ... RT-PCR products were purified using the PureLink Quick Gel Extraction Kit (Invitrogen) and identities confirmed by Sanger sequencing by Eurofins Genomics.
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bioRxiv - Immunology 2021Quote: ... 0.1μl SuperScript III RT/Platinum Taq (CellsDirect One-Step qRT-PCR kit; Invitrogen); 0.25μl pooled 0.5μM external Vb3 primers (Vb3 ...
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bioRxiv - Physiology 2022Quote: ... subjected to RT-PCR using High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific). Resulting cDNA was then utilized for qPCR using Taqman Q-PCR probes in QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... the TaqMan RNA-to-Ct One-Step-RT-PCR kit (Applied Biosystems, # 4392938) was used along with CHIKV primers and a FAM-probe targeting the nonstructural protein 4 (CHIKV Fwd IOLqPCR-6856F ...
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bioRxiv - Molecular Biology 2019Quote: ... miRNA mimics and the miR-100 isoforms miR-100-5p and miR-100-3p (all from Life Technologies) using 15 pmol PepMute reagent (SignaGen Labs ...
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bioRxiv - Cell Biology 2020Quote: ... Brilliant SYBR Green RT-PCR (Invitrogen) was used in the qPCR mix to amplify the cDNA product ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR grade water (Ambion #AM9935) was used for setting up all reactions ...
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bioRxiv - Microbiology 2023Quote: ... Superscript III RT-PCR system (ThermoFisher) was used to isolate cDNA for sequence analysis ...
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bioRxiv - Neuroscience 2023Quote: One step RT-PCR (Invitrogen #12594025) was used to isolate let-381 and unc-30 cDNAs (primers for let-381 ...
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bioRxiv - Microbiology 2021Quote: ... The recommended quantity of purified nucleic acids were amplified using real time PCR kits for PCV-2 (LSI VetMAX™ Porcine Circovirus Type 2 Quantification, Thermo Fisher Scientific, MA, USA), Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit ...
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bioRxiv - Microbiology 2021Quote: ... Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit; Thermo Fisher Scientific, MA, USA), Swine influenza virus [SIV] (EXOone Influenza A ...