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Citations for Thermo Fisher :
5751 - 5800 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Samples were resuspended in 50 mM HEPES and labelled using the 0.8 mg Tandem Mass Tag 10plex isobaric reagent kit (Thermo Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... truncation and a TEV protease cleavage site followed by a double maltose-binding protein-(MBP) tag at its C terminus was cloned into the pFastBac vector (Invitrogen). To obtain a SV1–Gs complex with good homogeneity and stability ...
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bioRxiv - Immunology 2021Quote: Protein Digestion and Tandem Mass Tag (TMT) labeling: The Pierce detergent compatible Bradford assay kit from Thermo Fisher (Winnipeg, MB) was used to determine the protein concentration ...
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bioRxiv - Cell Biology 2021Quote: The full-length cDNA of plexin-A2 was cloned into the gateway pDonor221 plasmid and then transferred by recombination into the pLenti6/V5-DEST lentiviral expression vector in frame with a C-terminal V5 tag according to the instructions of the manufacturer (Invitrogen), as previously described (Sabag et al. ...
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bioRxiv - Genetics 2020Quote: ... The tag permits the fragment to be directionally cloned into a pENTR entry vector (Thermo Fisher Scientific, Waltham, MA, USA); attL/attR recombination is used to transfer the correctly oriented fragment into a Gateway-compatible destination vector.
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bioRxiv - Biochemistry 2022Quote: ... containing N-terminal ten histidine tag (His-tag) and human rhinovirus 3C (HRV 3C) protease site was amplified by PCR and cloned into the pFastBac vector (Invitrogen) using seamless cloning method ...
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bioRxiv - Biochemistry 2022Quote: The T7 gp5.9 gene from phage T7 was produced as a synthetic gene construct with an N-terminal 3C-cleavable histidine tag (GeneArt, Invitrogen). This was subcloned into the pACEBAC1 (MultiBac ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... elegans) containing a C-terminal His6-tag was cloned into the polyhedrin promotor site of the pFastBac DUAL vector (Invitrogen). Baculoviruses for respective constructs were produced by transfecting Sf9-cells (Expression Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Peptides were collected by centrifugation and labeled using Tandem Mass Tag (TMTpro 16plex) reagents (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Detection of the eYFP-tag was performed by using primary rabbit antibodies against GFP (1:5000) (Invitrogen A6455, lot #1853896) and secondary anti-rabbit antibodies conjugated with horseradish peroxidase (1:10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... pMT-yki-HA was generated by cloning Yki-RG and HA tag into pMT-V5-His vector (Invitrogen, Cat# V412020). The Y281A ...
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bioRxiv - Synthetic Biology 2022Quote: Fluorescent proteins were purified in batch using His-tag affinity chromatography at room temperature according to the resin manufacturer’s instructions (Thermo Fisher). Lysates were supplemented with 10 mM imidazole and 600 μl was applied to HisPur Cobalt resin (300 μl ...
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bioRxiv - Cell Biology 2022Quote: ... The peptides were collected by centrifugation and isobaric labeling was performed using Tandem Mass Tag (TMT-10plex) reagents (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... They were produced with a histidine-tag in the Bac-to-Bac™ Baculovirus Expression System (Invitrogen, for VP3 proteins) or Escherichia coli prokaryotic system (for 2xFYVE) ...
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bioRxiv - Bioengineering 2024Quote: ... c-myc epitope tag and the variable heavy chain from mAbCII linked to the variable light mAbCII chain was procured from GeneArt (ThermoFisher). This fragment was cloned into a lentiviral expression plasmid we have previously described (31 ...
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bioRxiv - Biochemistry 2023Quote: Plasmids encoding each subunit of the BAM complex and OmpC genes (including a His6-tag) were transformed into BL21(DE3)* cells (Invitrogen). BamD and OmpC variant transformants were grown in LB (+ amp ...
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bioRxiv - Bioengineering 2024Quote: ... Antibodies were purified by gravity column using Protein G Sepharose 4 Fast Flow Resin (Cytiva) and proteins containing His tags were purified on Ni-NTA Agarose (R90101 Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... the secondary antibodies were chosen against the species of the primary antibody and with different Alexa Fluor color tags (1:500; goat anti-rabbit Alexa Fluor 488, A11004; goat anti-mouse Alexa Fluor 568; A11008, Invitrogen). The rest of the procedures were identical to 4.2.
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bioRxiv - Genetics 2024Quote: ... Sample preparation and labeling of the peptides with TMT isobaric mass tags was performed as manufacturer’s instructions (Thermo Fisher Scientific). In brief ...
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bioRxiv - Developmental Biology 2024Quote: ... or deleted CC (coiled-coiled) version of Bik-1 with an HA tag was inserted into the pcDNA3.1(+) backbone (Invitrogen: V79020). HEK293T cells were transfected with Lipofectamine™ 2000 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Sera competition ELISA assay plates were coated with PBS containing 250 ng of anti-6x His tag monoclonal antibody (Invitrogen) and left covered overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were resuspended in 50 mM HEPES and labelled using the 0.8 mg Tandem Mass Tag 10plex isobaric reagent kit (Thermo Scientific) resuspended in acetonitrile ...
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bioRxiv - Cell Biology 2023Quote: ... as per the manufacturer’s protocol) and a fluorescent Alexa Fluor tag (Alexa Fluor® succinimidyl esters, Invitrogen™, Molecular Probes® ...
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bioRxiv - Cell Biology 2023Quote: ... and MEAF6 were amplified by PCR using KOD One PCR Master Mix -Blue-(TOYOBO, KMM-201) and inserted into pcDNA3/GW with an HA or a FLAG tag (Invitrogen) or into pMAL-c6T (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was rinsed 2 times with water and incubated for 1 hour on a shaker at room temperature (RT) with the primary antibody (T7 tag, PA1-32386, Thermofisher) diluted in the blocking buffer (1:10,000) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Each sample containing a 100 μg peptide mixture was labeled using tandem mass tag reagent according to the manufacturer’s instructions (Thermo Scientific). The negative control group samples were labeled C1 ...
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bioRxiv - Microbiology 2023Quote: ... with a secretion sequence and C-terminal His6-tag) were transiently expressed using FreeStyle™ 293-F cells (Thermo Fisher) in FreeStyle™ F17 Expression Medium supplemented with L-glutamine and 1X MEM non-essential amino acids (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Microbiology 2022Quote: ... sE genes with a tandem C-terminal strep-tag in pMT/BIP/V5 plasmid were expressed in Drosophila S2 cells (Invitrogen) as described previously (20) ...
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bioRxiv - Molecular Biology 2023Quote: ... Experiments were carried out using 8 μL of SETDB1-3TD (residues 196-403, N-terminal His tag) and 20X concentration Sy-pro Orange dye (5000X DMSO stock, ThermoFisher) and 2 uL of ligand for a final concentration of 20 μM of protein and 200 μM of ligand with 1.1% of DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... Sequences of full-length Dprs and DIPs with an N-terminal V5 tag were cloned into the pMT/BiP/V5-His A vector (Invitrogen) for expression in S2 Drosophila cells for PLC cleavage assay or into the pAcGP67 baculovirus transfer vector (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NK-CD16 cells with positive SmBiT expression were selected via cell sorting by probing HA tag (anti-HA antibody [Invitrogen #26183 ...
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bioRxiv - Biophysics 2023Quote: ... in the N terminus and a hexahistidine tag at the C terminus was expressed in sf9 cells using the Bac-to-Bac baculovirus expression system (Invitrogen). SF9 cells at density a of 3.5 X 106 cells per milliliter were infected with mGlu5 virus grown for 48h at 27°C ...
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bioRxiv - Physiology 2023Quote: ... The sequence containing the complete open reading frame of mouse PNPLA3 fused at the 5’ end with sequence of a FLAG or Myc tag was amplified by PCR using Phusion DNA polymerase (ThermoFisher), and then cloned into DUAL2-CCM adenoviral shuttle vector (Vector Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... according to the published protocol 38optimized for higher proteome depth and 16-plex multiplicity using Tandem Mass Tag (TMT) pro (ThermoFisher) and using a gradient for thermal denaturation as described previously61,62.
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bioRxiv - Molecular Biology 2023Quote: For TMTpro-16plex labeling a set of 16 samples each containing 50 µg of peptides in 100 µL of digestion buffer (100 mm HEPES pH 8.3) was labeled using commercial TMTpro-16plex Mass Tag Labeling reagent kit (A44521, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and samples were run on reducing 17.5% Phos-tag gels (containing 50 μM Phos-tag Acrylamide AAL-107 (Wako) and 100 μM MnCl2) and NuPAGE 4–12% Bis-Tris gels (Invitrogen). All gels were stained with InstantBlue Coomassie Protein Stain (Abcam).
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bioRxiv - Cell Biology 2023Quote: ... PCR products of ame1 and okp1 point mutants (with C-terminal TAF tag and G418 marker) were amplified from the mutant plasmids using a high-fidelity DNA polymerase (SuperFi, Invitrogen) and transformed into the respective haploid ame1 and okp1 null mutants ...
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bioRxiv - Biochemistry 2023Quote: ... The protein:RNA mixture was then incubated with anti- c-myc agarose beads from a Pierce c-Myc Tag IP/Co-IP kit (ThermoFisher) for 2.5 hr ...
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bioRxiv - Evolutionary Biology 2023Quote: Codon-optimised sequences of Homo sapiens and Callithrix jacchus LEUTX with a GGGGSGGGGS linker and C-terminal V5 tag (Supplementary Figure S5) were synthesised by ThermoFisher GeneArt and cloned into a pcDNA3.1 mammalian expression vector ...
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bioRxiv - Biochemistry 2023Quote: ... The c-Myc tag labeling solution was prepared by a 1:100 dilution of c-Myc Alexa Fluor 488 conjugate (ThermoFisher) in PBS containing 0.2% BSA ...
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bioRxiv - Plant Biology 2023Quote: ... with a constitutive CaMV 35S promoter and either a YFP or CFP C-terminal tag using the LR Clonase II enzyme mix (Invitrogen). The YFP-tagged construct was used for the generation of stable transgenic Arabidopsis lines of and co-IP experiments ...
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bioRxiv - Neuroscience 2023Quote: ... A 6xHis-myc tag or GFP was attached to the N-terminus and the construct was cloned into the pSecTag2A vector (Invitrogen) in frame with the N-terminal IgK signal sequence ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 16 hours at 4°C with HA Tag-precoated Dynabeads-Protein G (Invitrogen, Cat.#: 10007D). Then the Dynabeads were rinsed with a high salt buffer (50mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... using a pDSG-IBA-Twin-Strep-Tag vector as template (IBA-lifesciences) into the HindIII-ApaI site of pcDNA5-FRT-TO (Invitrogen).
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bioRxiv - Developmental Biology 2024Quote: The PROX1 cDNA was subcloned into pFASTBAC1 to express PROX1 with an N-terminal FLAG- tag in Sf9 cells by the baculovirus expression system (Invitrogen). The PROX1-FLAG protein was then purified on M2 agarose (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... The elution of the complex was done via cleavage of the TAP-tag by incubation with the AcTEV Protease (Invitrogen) for 3 h at 17 °C in Buffer 30.