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Citations for Thermo Fisher :
5301 - 5350 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... then cooled at room temperature (RT) and 100μg/mL Ampicillin (Fisher Scientific) was added prior to pouring plates ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated for 30 min at RT with DAPI (Life Technologies) and the following secondary antibodies ...
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bioRxiv - Physiology 2021Quote: ... was transcribed to cDNA via High-Capacity cDNA RT Kit (ThermoFisher Scientific). Taqman Q-PCR probes were used in a QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Purified RNA was consecutively reverse transcribed (RevertAid RT Kit, Thermo Scientific #K1691). cDNA was diluted in water to a final concentration of 4 ng/μl.
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR assays were performed using SYBR Green Master Mix (Life Technologies). Gene expression was normalized to the expression of ELONGATION FACTOR1-ALPHA (EF1A) ...
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bioRxiv - Cell Biology 2021Quote: RT-qPCR was performed with TaqMan Fast Advance Kit (Fisher Scientific #4444963) and the following primers ordered from Integrated DNA Technologies ...
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bioRxiv - Genetics 2019Quote: ... The RT-qPCR was carried out in a QuantStudio6Flex machine (Applied Biosystems) using the following cycling conditions ...
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bioRxiv - Pathology 2021Quote: ... RT-qPCR was performed using an Applied Biosystems QuantStudio 3 (ThermoFisher Scientific) and the following cycling conditions ...
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bioRxiv - Immunology 2020Quote: ... and was reverse-transcribed with oligo(dT) and SuperScript II RT (Invitrogen) following the manufacturers’ protocols ...
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bioRxiv - Immunology 2021Quote: ... for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific) at medium shaking speed ...
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bioRxiv - Immunology 2020Quote: ... The 2× RT mastermix contained 1 μl 5× SuperScript II buffer (Thermofisher), 0.25 μl 100 mM DTT (Thermofisher) ...
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bioRxiv - Immunology 2021Quote: ... All RT-qPCRs were performed using a ViiA7 (Life Technologies, Paisley, UK), which included 40 cycles and a melt-curve ...
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bioRxiv - Immunology 2020Quote: ... The RT mastermix contained 0.25 μl 200 U/μl SuperScript II (Thermofisher), 0.25 μl 40 U/μl RnaseOUT (Thermofisher) ...
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bioRxiv - Immunology 2020Quote: RT-qPCR reaction TaqPath1-Step MultiplexMaster Mix was used (Cat.# A28527, ThermoFisher) along with the 2019-nCoV RUO Kit (Cat.# 10006713 ...
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bioRxiv - Microbiology 2020Quote: ... with a Reverse Transcriptase kit (High-Capacity cDNA RT Kit, Applied Biosystems). Per qPCR reaction ...
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bioRxiv - Immunology 2021Quote: ... The RT master mix consisted of: 0.2μL SuperScript IV 5x buffer (Invitrogen), 0.03μL SuperScript IV taq (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using Fast SYBR Green Master Mix (Thermo Fisher) for host gene expression or TaqMan Master mix (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run on a StepOnePlus™ RT-qPCR machine (Applied Biosystems). Average CT values of triplicate reactions were used to calculate expression changes ...
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bioRxiv - Neuroscience 2022Quote: ... Purified RNA was consecutively reverse transcribed (RevertAid RT Kit; #K1691 Thermo Scientific). cDNA was diluted in water to a final concentration of 3 ng/ml ...
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bioRxiv - Microbiology 2022Quote: RT-qPCR was performed using SYBR green master mix (Life Technologies, USA) as per manufacturer’s instructions and run using a CFX Max Real Time PCR machine (Bio-rad ...
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bioRxiv - Cell Biology 2022Quote: ... For RT-qPCR the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) was used in a LightCycler480 (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA transcripts were reverse transcribed using Maxima H-RT (Thermo Fisher EPO0753) in a template-switching-based RACE reaction ...
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bioRxiv - Cell Biology 2022Quote: ... RT was performed by using the Maxima Reverse Transcriptase protocol (Thermo Fisher) and qPCR analysis was carried out using specific primer pairs and SYBR green master mix (Kapa Biosystems ...
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bioRxiv - Genomics 2022Quote: ... 158.4 μL RevT buffer (5X, Maxima H Minus RT Kit, Thermo Fisher), 39.6 μL dNTPs (10 mM ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was performed using a QuantStudioTM 7 Flex System (Applied Biosystems) with the following amplification conditions ...
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bioRxiv - Genomics 2022Quote: ... 1 μg RNA was reverse transcribed with Revert Aid RT (Thermo Scientific). Assays were designed using PyroMark Assay Design SW 2.0 (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesised with SuperScript™ III Reverse Transcriptase (RT) kit (Invitrogen) using 5μL RNA and 1μL random hexamer primers (10μM ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems) and target specific primers (table 1 ...
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bioRxiv - Neuroscience 2023Quote: ... and RT-qPCR was performed using Power SYBR Green Master Mix (ThermoFisher). Primers were acquired from OriGene and the following sequences for NOX2 (Mus musculus Cybb ...
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bioRxiv - Genetics 2022Quote: ... RT-qPCR was performed with 2x SYBR Green MasterMix (ThermoFisher Scientific, USA) on a StepOne Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR reactions were performed using Maxima SYBR Green/ROX (ThermoFisher Scientific) as previously described (Nguyen et al. ...
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bioRxiv - Physiology 2022Quote: ... and used to produce oligo dT-primed cDNAs (SuperScript II RT, Invitrogen), which were then used as templates for quantitative real-time PCR ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems) and the specific pairs of primers for the neurotrophic factors listed in Table 1 ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using Fast SYBR Green Master Mix (Life Technologies) and the primers listed in Extended Data Table 5 were annealed at 61degC on the Step One Plus Real-Time PCR System (Life Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA conversion was performed using SuperScript-III RT kit (Thermo Fisher; 18080093) according to manufacturer’s instructions using 1 μg of extracted RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... reverse transcription (RT) was carried out with SuperScript III reverse transcriptase (Invitrogen) using 1 µg RNA and a mixture of 20 pmol random hexamer- and 4 pmol dT20-VN-primers in a 20 µl reaction at 50 D according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 500ng of RNA was used for reverse-transcription (MultiScribe RT – Applied Biosystems). Obtained cDNA was diluted to 2.5ng/uL and used as template for qPCR using a QuantStudio5 system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Scientific) was used and the manufacturer’s instructions were followed ...
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bioRxiv - Plant Biology 2024Quote: ... by reverse transcription with RT Maxima First Strand synthesis kit (Thermo Fisher) from 200 ng template RNA ...
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bioRxiv - Bioengineering 2024Quote: ... was incubated at RT for 20 mins with Lipofectamine™ RNAiMAX (ThermoFisher) in Opti-MEM™ and added to PerSCs in niches ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 μL of SuperScript™ III RT (200 units/μL, Invitrogen). The tubes were then incubated at 42°C for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... then blocked for 1h at RT in 5% NBCS (Gibco, #16010-159), antibiotic-antimycotic (Gibco ...
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bioRxiv - Genomics 2024Quote: ... 8 U/µl Maxima H Minus RT (Thermo Fisher catalog no. EP0752) and 2 U/µl Thermolabile Exonuclease I (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... 10 U/µl Maxima H Minus RT (Thermo Fisher catalog no. EP0752) and 5 µM of the respective barcoded oligo-dT primer ...
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bioRxiv - Molecular Biology 2024Quote: ... The RT reactions were performed with SuperScript IV reverse transcriptase (ThermoFisher, 18090010) following the manufacturer′s instructions using gene-specific primers ...
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bioRxiv - Microbiology 2023Quote: ... dNTPs (1 mM final concentration) and 20 U Maxima RT (Thermo Scientific) was added and RNA was reverse transcribed for 1 h at 50°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The RT was carried out with the RevertAid Reverse Transcriptase (Thermo Scientific) following the Manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Reverse transcription was performed using 200 U/μL M-MLV RT (Invitrogen) following the standard protocol as described by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Nucleus were stained with SYTO40 (10 μM, 5 minutes RT; Thermofisher, #S11351) to differentiate cells from axonal debris ...
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bioRxiv - Immunology 2023Quote: ... mRNA transcripts were reverse transcribed using Maxima H-RT (Thermo Fisher EPO0753) in a templateswitching-based RACE reaction ...