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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 5 μl Turbo DNase buffer and 3 μl Turbo DNase (ThermoFisher Scientific) were added to each reaction and incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 5 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific) were applied for monitoring effector caspase activation and 2.5 µM AlexaFluor647 hydrazide for detecting cell lysis.
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bioRxiv - Cancer Biology 2022Quote: ... Cell pellet was suspended in 3-5 ml TrypLE Express (ThermoFisher, 12605028) depending on the pellet volume and incubated at 37°C for 10-15 min with mixing every 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10% (for RS-5) or 20% (for DM-3) FBS (Gibco). All the other cells were cultured in RPMI medium (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were passaged every 3-5 days using Trypsin-EDTA (Gibco, 25200056). All experiments were done with cells at 10 passages or earlier with regular testing for mycoplasma ...
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bioRxiv - Cell Biology 2020Quote: ... 5′-ATTTGTCGACTCATTCTAATCCTTCGTCTTTTGATT-3′ by using Phusion high-fidelity DNA polymerase (Thermo Scientific) and cDNA prepared from the parasites as template ...
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bioRxiv - Cell Biology 2020Quote: ... were transfected with human myosin VI siRNA duplex (5′GGUUUAGGUGUUAAUGAAGtt-3′) (Ambion) or AllStars Negative Control siRNA duplex (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... LUVs were prepared using sonication (Fisher Scientific, Ultrasonic Bath, 3 × 5 min). Sonication was performed in ice water bath ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl Turbo DNase buffer and 3 μl Turbo DNase (ThermoFisher Scientific) were added to each sample and incubated for 30 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... TOGARAM1 was depleted using siRNA with sequence 5′-CCUCGUAAUUCCUUAGAAA-3′ (Thermo Scientific) Cells were seeded onto coverslips at 70% confluency and transfected with 50 nM of siRNA in two sequential transfections using Lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... ID: s2513 (Silencer Select Validated; 5’-GA UAUACCCUGGAAAGUCUtt-3’) (Thermo Fisher Scientific) with JetPrime (Polyplus ...
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bioRxiv - Molecular Biology 2024Quote: ... and cel-miR-54-3p (C. elegans sequence: 3’-UACCCGUAAUCUUCAUAAUCCGAG-5’, Invitrogen) were added to each sample and used as external controls.
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bioRxiv - Cell Biology 2024Quote: ... 3-4 or 5-6 and Phusion DNA polymerase (Thermo Fisher Scientific). The resulting PCR mix was DpnI digested and 4 μl of the mix was used for transformation of E ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hours of recovery in StemPro-34 SFM medium (Invitrogen) supplemented with 100 ng/ml Stem Cell Factor (SCF ...
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bioRxiv - Molecular Biology 2021Quote: ... A mixture of 40 pmol siRNA and 24 μl RNAiMAX (Invitrogen) were prepared with OptiMEM medium in a final volume of 160 μl ...
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bioRxiv - Genomics 2020Quote: ... DNA was purified using phenol:chloroform:isoamyl (25:24:1, Thermo Fisher Scientific) followed by the MiniElute PCR purification kit (Qiagen) ...
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bioRxiv - Systems Biology 2020Quote: ... and 24 μL Maxima H Minus Reverse Transcriptase (EP0753, Thermo Scientific). 3 * 107 cells were added to this mix ...
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bioRxiv - Microbiology 2020Quote: ... and incubated in 24-well plates (Nunc, ThermoFisher Scientific, Loughborough, UK) with Vero E6 cell monolayers ...
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bioRxiv - Cell Biology 2021Quote: ... All 24-hour treatments were supplemented with 0.70% FBS (CM3000, Gibco).
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted using phenol:chloroform:isoamyl alcohol (25:24:1) (Fisher Scientific) in Phase Lock Gel tubes (QuantaBio ...
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bioRxiv - Microbiology 2020Quote: ... and were extracted with phenol:chloroform:isoamyl alcohol (25:24:1, Thermo Scientific). DNA fragments were further purified by use of a PCR purification kit (Zymo) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using Phenol:Chloroform:Isoamyl Alcohol 25:24:1 (Fisher Scientific) and precipitated with GlycoBlue (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 24 h later cells were selected in 1μg/ml puromycin (Gibco) overnight ...
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bioRxiv - Microbiology 2020Quote: ... and 15 μl of a 0.1 μM SYTO 24 (ThermoFisher, S7559) stock in each well containing PBST and fixed bacilli ...
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bioRxiv - Biochemistry 2021Quote: ... Total RNA was extracted after 24 hours using Tri reagent (Invitrogen), followed by DNase I (NEB ...
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bioRxiv - Physiology 2021Quote: ... 20 and 24 hours with Arrayscan XTI (Cellomics, Thermo Fisher Scientific). After the final measurement ...
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bioRxiv - Immunology 2020Quote: ... 1 x 105 cells were plated onto 24-well plates (ThermoFisher). For assays of lysosomal damage ...
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bioRxiv - Genetics 2020Quote: ... for 24 hours before puromycine selection (in 1μg/ml puromycine [ThermoFisher]). Cells were deubiquitylated with MG132 (10μM ...
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bioRxiv - Cell Biology 2021Quote: ... 24 mM sodium bicarbonate and 0.5% AlbuMAX II (Gibco #11021-037). The medium was further complemented with 2 mM choline chloride (Sigma #C7527 ...
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bioRxiv - Plant Biology 2023Quote: ... and Methyl-PEG-Maleimide Reagent (MM(PEG)24) (Thermo Fisher, 22713). NEM was added to Chlamydomonas cultures growing as indicated in each case to a final concentration of 10 mM ...
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bioRxiv - Cancer Biology 2023Quote: 24 well tissue culture plates (#08-772-1, Thermo Fisher Scientific) were used for all proliferation assays ...
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bioRxiv - Cancer Biology 2023Quote: ... phenol:chloroform:isoamyl alcohol (25:24:1, v/v; Thermo Fisher Scientific 15593049) was used to extract nucleic acids ...
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bioRxiv - Immunology 2023Quote: Transwell inserts for 24-well plate (8 µm pore size, Nunc) were used and 250 µl of media containing chitinase-digested chitin flakes ...
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bioRxiv - Genetics 2023Quote: ... Cells were harvested after 24 hours using TRIzol Reagent (Invitrogen #15596026) and RNA isolation was performed according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and 24 h post treatment and mounted with DAPI (Invitrogen, #P36935). Imaging was performed using the ZEISS Celldiscoverer 7 Widefield microscope using a 20 x 1 magnification (NA = 0.8) ...
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bioRxiv - Microbiology 2024Quote: Caco-2 cells were seeded into 24-well plates (Thermo Fisher) at a density of 1.5×105 cells/cm2 and grown for 4 days before treatment with TMAO at varied concentrations for the experiment ...
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bioRxiv - Molecular Biology 2022Quote: ... for 24 h followed by selection with hygromycin B (Gibco, 10687010) at a final concentration of 50 μg/ml for 48 h ...
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bioRxiv - Bioengineering 2024Quote: ... after 24 h media was changed to Opti-MEM™ (ThermoFisher) and siRNA transfection was carried out 24 h later ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were labeled for 24 hours with RPMI medium (Gibco, #11879020) supplemented with 1 g/L [U-13C]-glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... and plated in domes in a 24-well plate (ThermoFisher #142475). After 10 minutes of incubation at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and were used in combination with 24-well plates (Thermo Fisher). The Caco-2 cells were grown in T75 flasks at 37°C in an incubator with an atmosphere of 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... Caco-2 cells were seeded into 24-well plates (Thermo Fisher) at a density of 1.5×105 cells/cm2 and grown for 4 days before treatment with TMAO at varied concentrations for the experiment ...
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bioRxiv - Cell Biology 2023Quote: ... coated 24 well plates (4EB/well; Fisher Scientific, 08-772-1C) with NK cell differentiation basal media supplemented with 5ng/ml IL-3 (1st week only ...
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bioRxiv - Immunology 2022Quote: ... 24 h before transfection by Turbofect (#Cat. No. R0533, Thermo Scientific,). The 4.7 kb vector (EGFPC1 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected for 24 hours with Turbofect reagent (Thermo Scientific) at a concentration of 2 μg for single plasmid transfections and 2 μg each of pCMV6 construct for co-transfections of TMEM163 and individual ZNT proteins tagged with either Myc-DDK or HA peptide (to equalize the plasmid concentrations across experiments) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected in 24-well dishes using Lipofectamine 2000 (ThermoFisher) with β-catenin reporter Super 8X TOPFlash and pSV-β-Galactosidase control vector ...
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bioRxiv - Bioengineering 2022Quote: ... 1 coverslips (Fisher Scientific, 50×24 mm, thickness 0.13 – 0.17 mm) to allow for imaging within the device using an oil-immersion 100x objective ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected 24 hours after seeding using LipoRNAimax (Invitrogen, 13778075) according to the manufacturer protocol with 80 nM siRNA (On-target plus Non-targeting or Human FAS pool ...
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bioRxiv - Biochemistry 2022Quote: ... the coverslips (24 mm x 40 mm, Thermo Scientific/Menzel-Glaser) were irradiated for 1 min under a deep UVs lamp (Ossila) ...
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bioRxiv - Cell Biology 2022Quote: ... Selection was started after 24 h with 0.5ug/ml Puromycin (Gibco). Growth of clones was boosted by supplementing the media with 1uM Copper until sufficient cells grew to freeze down and passage well ...