Labshake search
Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... 3′-end fluorescent labeling of the RNAs with fluorescein-5-thiosemicarbazide (ThermoFisher Scientific) was done as previously reported (Grozdanov and Stocco ...
-
bioRxiv - Microbiology 2020Quote: ... 3 to 5 colonies were transferred in Mueller-Hinton broth (Thermo Scientific Oxoid) and adjusted to an optical density at 600nm (OD ...
-
bioRxiv - Immunology 2020Quote: Pieces of 3 mm3 tumors were submerged in 5 vol of RNAlater (Invitrogen) (n = 5 samples/group) ...
-
bioRxiv - Developmental Biology 2020Quote: 3 × 106 ESC cells were distributed to 5 12-well dishes (Thermo Scientific) with 5 × 105 mESC cells per well ...
-
bioRxiv - Immunology 2022Quote: ... 5 μl RNA (2ng/μg) and 3 μl of 5x Primer stock (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... Amplifications were run on a QuantStudio 3 or 5 thermal cycler (Thermo Fisher) and results were analyzed using the instrument software ...
-
bioRxiv - Cell Biology 2023Quote: ... vector encoding a sgRNA targeting PAC (5′-TGTCGAGCCCGACGCGCGTG-3′) using Lipofectamine 2000 (Invitrogen). GFP positive cells were isolated by FACS two days after infection ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells were passaged every 3-5 days using Accutase or ReleSR (ThermoFisher Scientific).
-
bioRxiv - Molecular Biology 2023Quote: ... 3 × 10-4 M MTG and 5% Protein-Free Hybridoma Media II (Gibco).
-
bioRxiv - Cancer Biology 2023Quote: 5’ and 3’ RACE was performed using the GeneRacer kit (Thermo Fisher Scientific), following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... 200 nM MCPH1-5’-CUCUCUGUGUGAAGCACCUdTdT-3’) in serum-Free Opti-MEM medium (Gibco). The transfection controls were set up as above but without adding the siRNA oligos ...
-
bioRxiv - Microbiology 2024Quote: ... 5’-TATTGGTCTCAGGGAGCGAAAGCAGG 3’ using Superscript III according to the manufacturer’s protocol (Invitrogen, #18080400).68 PCRs were performed to amplify and append partial Illumina i5 and i7 adapter sequences across four sub-amplifications of each library to sequence the entire vRNA genome ...
-
bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
-
bioRxiv - Biophysics 2024Quote: ... and 3-pyridinemethanol (RI = 1.545, 3-PM, A10381, Thermo Fisher Scientific) were used to mix with the water-based SMLM buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2020Quote: ... Genomic PCR products obtained with primers 5’-CGCCATCAACTTCACCTAGC-3’ (forward) and 5’-TCTGGGAAGAAGTTTGGCCT-3’ (reverse) were sequenced using the BigDye® Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Massachusetts, USA) on an ABI 3130xl Genetic Analyzer (Life Technologies ...
-
bioRxiv - Cell Biology 2021Quote: ... a probe prepared by PCR on genomic mouse DNA with the primers 5’-CATATTCCAGGTCCTTCAGTGTGC-3’ and 5’-CACTTTAGGACGTGAAAT ATGGCG-3’ was labeled with Cy3 by random priming according to the kit instruction (Invitrogen Kit, Ref 18095–011).
-
bioRxiv - Bioengineering 2021Quote: ... sgRNA LA93527 was generated from a PCR DNA template (overlapping primers LA935 5’-GAAATTAATACGACTCACTATAGGACAGTGCGGTCCG-CAAGGGTTTTAGAGCTAGAAA-3’ and LA137 5’-AAAAGCACCGACTCGGTGCCA-CTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC-3’) containing the T7 promoter using the MEGAscript T7 Transcription kit (ThermoFisher Scientific, Walthum, MA USA). The reaction mix was incubated at 37°C for 16 hours and purified using the MEGAclear Transcription Clean-Up kit (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... TERT immortalisation was maintained in the presence of 5 µg/ml of Hygromycin (Invitrogen, 10687-010). Cells transduced with lentiviruses were maintained in media supplemented with 1 µg/ml Puromycin (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... and measured every 12 or 24 hours by adding alamarBlue HS (Invitrogen) (1/20 ...
-
bioRxiv - Microbiology 2022Quote: ... were added to a 24-well plate (Nunc, Thermo Scientific, Asse, Belgium). Then ...
-
bioRxiv - Molecular Biology 2020Quote: ... for 24 hours before transfecting with RNAiMax (Thermo Fisher Scientific: 13778-150) with 20nM siRNA in Opti-MEM (Gibco ...
-
bioRxiv - Genetics 2021Quote: ... 400 µL of acid phenol:chloroform:isoamyl alcohol (125:24:1; pH 4.7; Ambion) and 200 uL of acid-washed glass beads (0.5 mm ...
-
bioRxiv - Synthetic Biology 2021Quote: ... DNA was purified with phenol:chloroform:isoamyl alcohol (25:24:1; vol/vol) (Invitrogen) followed by an ethanol precipitation ...
-
bioRxiv - Cell Biology 2021Quote: Cells were harvested 24 hours post transfection with 0.05% Trypsin-EDTA (Gibco) from a 6-well plate and reseeded on Nunc Permanox chamberslides ...
-
bioRxiv - Developmental Biology 2020Quote: ... DNA was purified with phenol:chloroform:isoamyl alcohol (25:24:1; vol/vol) (Invitrogen) and by performing ethanol precipitation ...
-
bioRxiv - Neuroscience 2021Quote: HEK293 cell line were plated in 24-well plates in DMEM (Invitrogen) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Immunology 2021Quote: ... were used to assign 24 discrete glycan peaks using GlycanAssure software (ThermoFisher). Data are reported as percentages of total glycans for each of the glycan peaks.
-
bioRxiv - Bioengineering 2021Quote: ... Genomic DNA was extracted with phenol:chloroform:isoamyl alcohol (25:24:1, Fisher Scientific) and precipitated using 70% ethanol (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2020Quote: ... in a 24-well plate containing 1.5 ml Neurobasal-A medium (Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... 24 hours after transfection cells were selected with 200µg/ml HygromycinB (ThermoFisher) to select stable transformants ...
-
bioRxiv - Cell Biology 2020Quote: ... and grown for 24 hours in live-cell imaging medium (Fluorobrite (Invitrogen), 4 mM L-Glutamine ...
-
bioRxiv - Microbiology 2022Quote: ... An equal ratio of sample and 24:1 chloroform:isoamyl alcohol (Acros Organics) were used for extractions ...
-
bioRxiv - Immunology 2022Quote: ... and SYTO 24 Green Fluorescent Nucleic Acid Stain (1:1000, S7559, Invitrogen) was reported over time ...
-
bioRxiv - Microbiology 2022Quote: ... were added to a 24-well plate (Nunc, Thermo Scientific, Asse, Belgium). Then ...
-
bioRxiv - Cancer Biology 2022Quote: ... treated 24 hours post-treatment and 0.08 μg/ml colcemid (KaryoMAX, Invitrogen) was added 20 hours before harvest ...
-
bioRxiv - Microbiology 2020Quote: ... 24 mM sodium bicarbonate and complemented with 0.5% Albumax II (Life Technologies). NF54 parasites were cultured with 10% AB+ human serum instead of 0.5% Albumax II ...
-
bioRxiv - Molecular Biology 2020Quote: HEK 293 cells (24 well format) were transfected with Lipofectamine 3000 (Invitrogen) following manufacturers protocol ...
-
bioRxiv - Microbiology 2020Quote: Samples were incubated in 24-well plates (Nunc, ThermoFisher Scientific, Loughborough, UK) containing twice washed with Dulbecco’s PBS (DPBS ...
-
bioRxiv - Bioengineering 2021Quote: Glass coverslips with dimensions 50×24×0.15 mm (Menzel Glazer, Thermo scientific) were spin-coated (1500 rpm ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were seeded onto glass coverslips in 24-well dishes (Nunc, Denmark) before various treatments ...
-
bioRxiv - Microbiology 2021Quote: ... FISH was performed 24 hours later using ViewRNA Cell Plus Assay (ThermoFisher) as manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... placed in 24 well plates (#142485, Thermo Fisher Scientific, Waltham, MA, USA) already containing 1 mL of Maintenance Medium or Pluricytes Cardiomyocyte Medium per well ...
-
bioRxiv - Synthetic Biology 2020Quote: ... All transfections were performed in 24-well plates using Lipofectamine 2000 (Invitrogen).
-
bioRxiv - Physiology 2021Quote: ... Hepatocytes were plated in collagen-coated 24-well plates (Thermo Fisher Scientific) in William’s Medium E (PAN-Biotech ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 μL of RNase A (24 mg/mL stock; Thermo Scientific). Eluted DNA was isolated using a Qiagen PCR purification kit (Qiagen ...
-
bioRxiv - Genetics 2022Quote: ... Worms were lysed using the Bead Mill 24 homogenizer (Thermo Fisher Scientific). Bromochloropropane was added to the lysis to perform RNA extraction ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Samples were homogenized at 4ºC using a Beadmill 24 Homogenizer (Thermofisher Scientific) with the following settings ...
-
bioRxiv - Biochemistry 2022Quote: ... 24 h post infection cells were PF using the Vitrobot (Thermo Fisher) offsetting the blotting pad to favour back blotting ...