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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... transferred to 6 ml liquid MSgg medium of each well of a 6-well microplate (Thermo Scientific), and then grown for additional four days ...
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bioRxiv - Microbiology 2020Quote: ... 6 × 105 293LTV cells were seeded in a 6-well plate and transfected using Lipofectamine 2000 (Invitrogen) which was complexed with DNA plasmids driving the expression of either VSV G protein (positive control) ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Biophysics 2023Quote: 6-well plate cell proliferation – Cells were seeded into 6-well plates (Fisher Scientific, 07-200-83) 150,000 cells/well and left at ambient temperature for 20 minutes to ensure homogeneous settling ...
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bioRxiv - Cell Biology 2023Quote: ... Coding regions were transferred into Pvha-6-GFP or Pvha-6-RFP vectors by LR reaction (Invitrogen). Constructs were bombarded into unc-119(ed3 ...
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bioRxiv - Molecular Biology 2020Quote: ... whole cell lysate was collected by centrifugation at 13,000 × g for 15 min at 4 °C and treated with 5 U/ml RNase T1 (Thermo Fisher Scientific) and Turbo DNase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... 10,000 freshly sorted EpCAM+ DC were transfected with 10pmol of a pool of 4 ON-Targetplus siRNA (Dharmacon) (see Supplementary Table 5) for each candidate gene using Lipofectamine RNAimax (Life Technologies) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from WT and MafAS64F/+ islets (n=4 for males, n=5 for females) using the RNAqueous total RNA isolation kit (Ambion; Thermo Fisher), and then analyzed on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates in the SDS-PAGE sample buffer were boiled for 5 min before separating the protein on 4-12% gradient gels (Bolt Bis-Tris Plus Gels, Thermo Scientific). Proteins were then transferred onto PVDF membranes (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... was denatured at 70°C for 5 min and resolved on 4-20% Tris-borate-EDTA (TBE) Urea gel (ThermoFisher scientific) in cold 0.5 × TBE buffer (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... secondary antibodies diluted to 4 μg/ml in PBS containing 5 μg/ml Hoechst 33342 dye (Molecular probes, cat no. H3570). After treatment with secondary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were analyzed by SDS-PAGE under reducing conditions (5% β-mercaptoethanol) on 4-12% Bis-Tris NuPage Gels (Thermo Fisher) and cleavage products were detected using mouse monoclonal BC-3 antibody which detects aggrecan cleavage at the Glu392↓Ala393 bond (Cat n. ...
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bioRxiv - Biochemistry 2020Quote: ... Successful enrichment was validated by running 5 ul sample elution on NuPAGE 4-12% Bis-Tris gels followed by silver stain (ThermoFisher LC6070) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked in PBS-T containing 5% NDS and then incubated overnight at 4°C with rabbit anti-PSD95 (1:1000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 (1:10,000 ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 1.5 μg of rRNA subtracted total RNA or 10 μg of in vitro-transcribed RNA was added to 4 μl 5× first strand buffer (ThermoFisher Scientific), 1 μl 10μM reverse primer ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 1.5 μg of rRNA subtracted total RNA or 10 μg of in vitro-transcribed RNA was added to 4 μl 5× first strand buffer (ThermoFisher Scientific), 1 μl 10μM reverse primer ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was rewashed with TTBS for 4 x 5 min and was developed for chemiluminescence (with agent SuperSignal West Pico, Thermo Scientific) using a LAS 4000 Image Analyzer (ImageQuant).
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bioRxiv - Neuroscience 2020Quote: Murine cortical cultures in control or supplemented media were cultured for 4 passages before being dissociated into a single cell suspension and stained with 5 µM CFSE (Molecular Probes) in PBS at 37°C for 15 minutes in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... and heated at 95ºC for 5 minutes and resolved using a 4-20% tris-glycine gel (ThermoFisher Scientific, Waltham MA, XP04200). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged for 3 min at 300g at 4°C and resuspended in 1 ml pre-warmed enzymatic mix of 5 mg/ml Dispase (Gibco, 17105041) and 0.5 mg/ml Liberase TH Research Grade (Roche ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from 5 HIOs per group with a total of 4 replicates per condition using the mirVana miRNA Isolation Kit (Thermo Fisher). The quality of RNA was confirmed ...
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bioRxiv - Molecular Biology 2022Quote: ... then the supernatant was diluted 10x and pre-cleared for 30 min at 4 °C with rotation using 5 µl protein A- and G-coupled dynabeads (ThermoFisher Scientific). An input sample was taken ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were cleared by centrifugation at 16,000 × g for 5 min at 4°C and protein concentration was determined using the Pierce BCA protein assay kit (Thermo Scientific). Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 21,000g for 20 min at 4 °C and 5 μl/ml of RNase A/T1 (Thermo Fisher Scientific) was added to the supernatants ...
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bioRxiv - Pathology 2020Quote: ... and resolved on 4-12% Bis-Tris Plus Bolt™ gels at 4-5 ug of protein per lane in MES SDS Running Buffer (Invitrogen). Target-protein signal was detected with Horseradish Peroxidase (HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were heated to 95 °C for 5 min and cooled on ice prior to loading on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher). SDS-PAGE was performed of all compared samples in parallel according to the manufacturer’s specification ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated in ice for 5 min and then loaded into to a pre-cast gel (NuPAGE® 4-12% Bis-Tris Protein Gels, Invitrogen). Gel electrophoresis was performed for 90 min at 125V ...
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bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
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bioRxiv - Immunology 2020Quote: ... and heated at 70°C for 5 min before separation on 4-12% Bis-Tris Plus ready gel (Thermo Fisher Scientific). To determine PGE2R glycosylation in the hemolymph samples ...
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bioRxiv - Neuroscience 2021Quote: ... prepared in 5% NGS in DPBS overnight at +4 following an incubation with Alexa Fluor405 (A31556, Thermo Fisher Scientific, 1:500) or Alexa Fluor680 (A31556 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBS-0.5%Triton X-100 at RT before overnight incubation at 4°C with Alexa555-conjugated donkey-anti-rabbit antibody (Invitrogen, #A32794) in PBS containing 5% NHS ...
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bioRxiv - Developmental Biology 2021Quote: ... Collected cells were then pelleted at 0.8 × 1000g for 5 minutes at 4 degrees Celsius and resuspended in Trizol (Thermo Fisher 15596018). After genotyping ...
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bioRxiv - Biochemistry 2021Quote: ... and insoluble samples and were denatured in reducing Laemmli buffer at 100°C for 5-10 min prior to loading onto a 4-20%Tris-Gly SDS-PAGE gel (Life Technologies). Proteins were then transferred to a nitrocellulose membrane using an iBlot2 (Life Technologies) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Media was exchanged daily and splitting was performed every 4-5 days when cells reached 75-85% confluency using TrypLETM (Gibco, 12605028). Cultures were propagated for at most 4 passages before being replaced by fresh cell stock.
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bioRxiv - Microbiology 2022Quote: Cultures taken from exponential growth at OD 0.5 are starved according to the above protocol and stained with 5 μg/ml DiBac3(4) and 1.25 μg/ml Propidium Iodide (Thermo Fisher Scientific) 10 minutes after entry to starvation ...
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bioRxiv - Biochemistry 2022Quote: ... The gel was then washed 4 times for 5 minutes each with deionized water and stained with Imperial Blue stain (ThermoFisher #24615) for two hours before washing in deionized water overnight ...
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bioRxiv - Microbiology 2022Quote: ... Q-PCR was performed using PowerUp SYBR Green Master Mix with 4 replicates and an Applied Biosystems QuantStudio™ 5 real-time PCR instrument (ThermoFisher). E ...
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bioRxiv - Neuroscience 2022Quote: ... Cortical neurons were pelleted at 2,500 x g for 5 minutes at 4 °C and resuspended in 1 mL Neurobasal outgrowth media (Thermo Scientific, 21103049), supplemented with B-27 (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with excess DTT was added to all reactions and samples were fixed for 5 min at 95°C before loading on a 4-12% Bis-Tris gel (Invitrogen WG1402BOX). The same samples without BS3 were run as a loading control and stained using a coomassie protein stain (Abcam ab119211 ...
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bioRxiv - Molecular Biology 2022Quote: IP samples were eluted with 40 µl 1xLDS buffer adjusted to 10 mM DTT (5 min, 90°C) and separated on 4-12% Bis-Tris NuPAGE gels (Thermo Fisher) followed by colloidal coomassie staining (Instant Blue ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... with 5% β-mercaptoethanol at 50°C for 10mins and loaded onto a 4-20% Tris-glycine SDS-PAGE gel (Invitrogen). The proteins were transferred to a nitrocellulose membrane with an iBlot (ThermoFischer) ...
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bioRxiv - Biochemistry 2022Quote: Phagosomes or cells were lysed directly in 2x Laemmli buffer (with 5% ß-mercaptoethanol) and subjected to SDS-Page using 4-12% NuPAGE gels (Invitrogen) and immunoblotted to PVDF membrane ...
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bioRxiv - Neuroscience 2022Quote: ... 800 µL of the bottom phase was transferred to scintillation vials containing 5 mL ScintiVerse BD Cocktail (Fisher Scientific SX18-4). The vials were vortexed and radioactivity was measured in a Scintillation Counter (Tri-Carb 2819TR ...
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bioRxiv - Bioengineering 2022Quote: ... the samples were transferred to the dialysis wells plates and incubated at 37°C and 5% CO2 for a total 4 weeks in osteogenic monoculture medium containing DMEM (low glucose, 22320, Thermo Scientific), 10% human platelet lysate [11] (hPL ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... the neurons were given 12 hours in growth media at 37°C/5% CO2 to allow sufficient recovery and expression of the plasmid prior to fixation in 4% formaldehyde (Thermo Fisher) / 4% sucrose (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... zebrafish larvae from visually similar developmental stages were collected in groups of 16 individuals from all corresponding genotypes (N = 4-5 per genotype) and stored in RNAlater (Thermo Fisher) until use ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-20 μg of total protein was separated by NuPAGE 4-12% Bis-Tris Mini Protein Gels (NP0336BOX; Thermo Fisher Scientific) and transferred to Immobilon-P PVDF Membrane (IPVH00010 ...
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bioRxiv - Genomics 2024Quote: ... The purified DNA solutions from the eight aliquots were combined and loaded into 5 lanes of 4% E-gel (Invitrogen, G401004). The DNA bands with the size between 300 bp and 1.2 Kb were excised ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then incubated overnight at 4 °C with 5 μg of prebound anti-SP100 or rabbit IgG isotype control protein A dynabeads (Thermo Fisher). The next morning ...
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bioRxiv - Immunology 2023Quote: ... at 1:100 overnight at 4°C in 5% BSA in the presence of 0.05% sodium azide (BP922I-500; Thermo Fisher Scientific), 0.5% Triton X-100 (161-0407 ...