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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... the 3’ end of rpc37 was first amplified by PCR and cloned into pCR Blunt II-TOPO using the Zero Blunt II TOPO PCR Cloning Kit (Invitrogen Life Technologies). Site-directed PCR mutagenesis was then carried out to mutate the codon corresponding to valine 189 (GTC into GAC ...
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bioRxiv - Biophysics 2020Quote: Madin Darby canine kidney II (MDCK-II) (ECACC, Cat. No. 00062107) and MDCK Myr-Palm-GFP H2B-mCherry cells were cultured in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... chloride hemipentahydrate (Cd2+), lead (II) nitrate (Pb2+), mercury (II) chloride (Hg2+), and hydrogen peroxide (H2O2, 30% in water) were purchased from Fisher Scientific. Buthionine sulfoximine (BSO) ...
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bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... supplemented with 10% foetal bovine serum (FBS) (PAA Laboratories, Pasching, Austria) and 1% pen/strep (Gibco/Fisher Scientific, Hampton, New Hampshire, USA) at 37 °C and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... supplemented with 10% foetal bovine serum (FBS) (PAA Laboratories, Pasching, Austria) and 1% pen/strep (Gibco/Fisher Scientific, Hampton, New Hampshire, USA). Cells were incubated for 24 h ...
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bioRxiv - Biophysics 2022Quote: ... The cell culture medium (RPMI 1640) and complements (10% FBS, 1% Hepes 1M, 1% Glutamax, 1% Pen/Strep) were obtained from Gibco (Life technologies). Cells were monthly tested for the presence of mycoplasma.
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bioRxiv - Bioengineering 2022Quote: ... Prepared drug stocks were then diluted 1:100 in cell maintenance media (Gibco DMEM supplemented with 10% heat-inactivated FBS, 1% Gibco NEAA, 1% Gibco Pen-Strep) to achieve a 2x concentration ...
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bioRxiv - Cell Biology 2019Quote: ... Following tissue digestion 8 ml of resuspension media (DMEM/F12 with glutamax supplemented with 15%FBS and 1% pen/strep; Thermo Scientific, CA) was added to stop enzyme activity ...
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bioRxiv - Neuroscience 2020Quote: ... Embryo hippocampi were dissected in 4 °C Hibernate E supplemented with 2% B27 supplements and 100 U/mL Penicillin/Strep (Thermo Fisher Scientific). Hippocampal tissues were digested in Hibernate E containing 20 U/mL papain ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% FBS, 1% pen/strep, 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (Thermo Fisher Scientific). Cell lines were validated by STR-typing ...
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bioRxiv - Cell Biology 2021Quote: ... enzyme activity was stopped with 8 ml of resuspension media (DMEM/F12 with glutamax supplemented with 15%FBS and 1% pen/strep; Thermo Scientific, CA). The digestion mixture was passed through 100 μm cell strainer and centrifuged at 150 x g for 8 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Prepared 200X dilutions were then diluted to 2X concentration in infection media (Gibco DMEM supplemented with 5% HyClone FetalCloneII, 1% Gibco NEAA, 1% Gibco Pen-Strep). Growth media was removed and cells were pretreated with 2 X drug for 1 hour prior to infection at 37C and 5% CO2 ...
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bioRxiv - Cancer Biology 2019Quote: ... and 10 U/mL of penicillin G sodium and 10 mg/mL of streptomycin sulfate (pen-strep; Gibco/Invitrogen, Waltham, MA; 15140122). BT549 BCa cell line was grown in RPMI-1640 medium (Hyclone ...
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bioRxiv - Cancer Biology 2019Quote: ... and 10 U/mL of penicillin G sodium and 10 mg/mL of streptomycin sulfate (pen-strep; Gibco/Invitrogen, Waltham, MA; 15140122). BT549 BCa cell line was grown in RPMI-1640 medium (Hyclone ...
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bioRxiv - Molecular Biology 2021Quote: ... All tissues were incubated at 37°C in pre-warmed chick heart media (α-MEM supplemented with 10 % FBS and 1% penn-strep, Gibco, #12571) for at least 1 h for stabilization ...
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bioRxiv - Cell Biology 2020Quote: ... (PCS-201-013) were purchased from ATCC and cultured in 10% Fetal Bovine Serum (FBS) and 1% pen-strep (Gibco, Life Technologies) in Dulbecco’s modified Eagle Medium (DMEM ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed with PBS and blocked with R10 media (RPMI-1640 with 10% FCS, 1% Pen/Strep, 2mM L-Glutamine (all from Thermo Fisher Scientific)) for 30 minutes at RT before the addition of freshly isolated PBMCs ...
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bioRxiv - Immunology 2020Quote: ... catalog number: CRL-2522) were cultured in Eagle’s Minimum Essential Medium with 10% FBS and 1% Pen-Strep (Gibco, catalog number: 15140122). Human ventricular cardiac fibroblasts that were harvested from normal adult ventricular tissue were obtained from Lonza ...
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bioRxiv - Immunology 2022Quote: ... or monoclonal antibodies (final concentration of 100 μg/ml) in RPMI 1640 media supplemented with 10% FBS 1% Pen/Strep (Gibco, Gaithersburg, MD) for 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 2.5e6 A375s were plated per T75 flask in complete RPMI media (RPMI plus NEAA, Glutamine, Hepes, Pen/Strep, sodium pyruvate (all Thermo Fisher Scientific) and 10% FCS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% Cytiva HyClone Fetal Clone III (FC3; Cytiva Life Sciences, Marlborough, MA) and 1% Penicillin-Streptomycin (Pen Strep; Invitrogen, Carlsbad, CA) at 37 °C in 5% CO2 ...
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bioRxiv - Genetics 2024Quote: Cortices were dissected from E16.5 C57BL/6J embryos and cultured in supplemented neurobasal medium (Neurobasal [Gibco 21103-049], B27 [Gibco 17504044], GlutaMAX [Gibco 35050-061], Pen-Strep [Gibco 15140-122]) in TC-treated 12- or 6well plates coated with 0.05 mg/mL Poly-D-lysine (Sigma-Aldrich A-003-E) ...
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bioRxiv - Developmental Biology 2024Quote: ... The intestine was placed in a dish containing pre-cooled D-PBS supplemented with 1% (vol/vol) Penicillin/Streptomycin (Pen/Strep; Gibco, cat.# 15070063), and fat and mesenteric tissue were removed ...
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bioRxiv - Cell Biology 2023Quote: ... -H [fused with a Twin-Strep tag at its C-terminus]) were codon-optimized for Trichoplusia ni and synthesized (GeneArt, Thermo Fisher Scientific). They were inserted stepwise into intermediate plasmid vectors (namely ...
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bioRxiv - Molecular Biology 2023Quote: ... Mammalian expression constructs for GFP-VCF1 WT and Strep-HA-VCF1 were generated by PCR amplification of a synthetic VCF1 cDNA isoform (Thermo Fisher Scientific) encoding isoform 1 of human FAM104A (UniProt ID ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were allowed to recover for approximately 2 hours in petri dishes filled with E2 buffer supplemented with penicillin/streptomycin (Pen/Strep, 100 unit, 100 ug/mL, Gibco #15140-122), before mounting and imaging (Briona and Dorsky ...
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bioRxiv - Cell Biology 2023Quote: ... intestinal fragments were incubated in epithelial cell solution (10 mM EDTA [Invitrogen, #15575020]-HBSS, 5 mM HEPES [Invitrogen, #15630080], 100 U/ml Pen-Strep [Invitrogen, #15140122], and 2% FBS [Life Technologies, #26140079]) for 30 min on ice with continuous gentle shaking ...
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bioRxiv - Cell Biology 2023Quote: ... resulting in pDONR221-ECFP-DVL3 vector that was combined with pDEST-Twin-Strep-Flag-HALO via Gateway Technology (Thermo Fisher Scientific #11791020). HALO tag was subsequently removed by site-directed mutagenesis ...
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bioRxiv - Neuroscience 2023Quote: ... with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63]) and transfected them when 50-70% confluent ...
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bioRxiv - Molecular Biology 2023Quote: ... All tissues were incubated at 37°C in pre-warmed chick heart media (α-MEM supplemented with 10% FBS and 1% pen-strep, Gibco, #12571) for at least 1 hour for stabilization ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 100 units/ml penicillin and 100 µg/ml streptomycin (pen/strep; Biological Industries®) and 8 % fetal bovine serum (Gibco®). Via transduction of hACE2-4xMyc gene in HEK293T and selection with 15 µg/ml blasticidin ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell culture media were supplemented with Penicillin/Strep (100units/mL Penicillin, 100micrograms/mL Streptomycin) and MEM media was additionally supplemented with 2mM glutamine (Gibco 25030-081).
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bioRxiv - Cell Biology 2021Quote: ... Total proteins were quantitated using Qubit Protein Assays (ThermoFisher) and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX ...