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Citations for Thermo Fisher :
5301 - 5350 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 5303 SPRN SPRN-5) was biotinylated using the EZ-Link NHS-PEG4 Biotinylation Kit (Thermo Scientific, catalog No. PI21455) and used at a concentration of 1µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... both RBD and HEL were site-specifically biotinylated in house using EZ-link maleimide-PEG2-biotin (ThermoFisher, CAT#A39261) so that the binding epitopes on these proteins were not modified ...
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bioRxiv - Molecular Biology 2023Quote: ... an amino acid analog of methionine containing an alkyne moiety that can be biotinylated by click-it chemistry (ThermoFisher), for nascent protein translation labeling followed by SDS-PAGE and HRP-streptavidin Western analysis ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated RNA was purified also as described (Duffy and Simon, 2016) using 80 μl MyOne Streptavidin C1 Dynabeads (Invitrogen) + 100 μl biotinylated RNA for 15 min at RT with rotation and under foil ...
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated hSA4 antibody (in-house antibody) was generated using 200 μg Sulfo-NHS-LC Biotin (Pierce, Thermo Fisher Scientific), diluted 1:200 in blocking buffer and added to the plate for 1 hour at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... sections were washed 3 times with PBS and incubated at room temperature with biotinylated universal secondary antibody (Life technologies). After washing with PBS x3 ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated IgG retained on plates was reacted with a 1:10,000 dilution Streptavidin-HRP conjugate (Thermo Scientific, cat. N504) in ADA-WB containing 10 mg/ml BSA for one hour at 37°C ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... Pellet 1 was subjected to a second round of digestion in 1 mL of 1000 U/mL Collagenase type II and 1 mL of 11 U/mL Dispase II (Thermofisher, Cat# 17105041) along with the 8 mL of the remaining cell suspension ...
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bioRxiv - Plant Biology 2020Quote: ... the fragments were cloned into entry vector pDONR207 and yeast expression vector pYesdest52 using Gateway BP Clonase II Enzyme Kit and LR Clonase II Enzyme Kit (Invitrogen, MA, USA), respectively.
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bioRxiv - Molecular Biology 2019Quote: ... The PCR fragment obtained was cloned into pCR Blunt II TOPO using the Zero Blunt II TOPO PCR Cloning Kit (Invitrogen Life Technologies) and sequenced.
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bioRxiv - Molecular Biology 2019Quote: ... the 3’ end of rpc37 was first amplified by PCR and cloned into pCR Blunt II-TOPO using the Zero Blunt II TOPO PCR Cloning Kit (Invitrogen Life Technologies). Site-directed PCR mutagenesis was then carried out to mutate the codon corresponding to valine 189 (GTC into GAC ...
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bioRxiv - Genomics 2024Quote: ... Emulsion PCR for sequence template synthesis was performed using the Ion PITM Hi-QTM OT2 200 Kit v2 with the Ion One TouchTM II System and the Ion One TouchTM II ES (Thermo Fisher Scientific). Sequencing was performed using the Ion ProtonTM System with Ion PITM Chip kit v3 (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... Emulsion PCR for sequence template synthesis was performed with the Ion PITM Hi-QTM OT2 200 Kit v2 using the Ion One TouchTM II System and the Ion One TouchTM II ES (Thermo Fisher Scientific). Sequencing was performed using the Ion PITM Chip kit v3 and the Ion ProtonTM System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: mouse anti-V5-tag to amplify V5-tagged CFP (Thermo Fisher, R96025, 1:650), rat anti-mCherry (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... Chicken anti-rabbit tagged with Alexa Fluor 488 and goat anti-mouse tagged with Alexa Fluor 568 were used as secondary antibody at a dilution of 1:1000 (Invitrogen). Tissues were incubated with these secondary antibodies for 48 hours at 4 °C followed by regular washing ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged full-length TRIM21 was cloned into a pFastBac vector for expression in the baculovirus system (Bac-to-Bac, Invitrogen), resulting in pFastBac-MBP-TRIM21 ...
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bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... with Strep and His epitope tags was amplified by PCR from pIEx10-LRIM1 (Povelones et al. 2009) with BamHI tagged primers (LRIM1-F, LRIM1-R) and subcloned into pJET1.2 (Thermo Scientific) before cloning into pslVg-BGH-attP ...
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bioRxiv - Molecular Biology 2019Quote: BGH was amplified by PCR from pslVg-GFP-BGH by PCR with BamHI and NotI tagged primers (BGH-F, BGH-R) and subcloned into pJET1.2 (Thermo Scientific). attP was amplified from pBCPB+ (Groth et al ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid encoding for FLAG-tagged TM or IM-TM were transfected into EXPI293F cells according to the manufacturer (Thermo Fisher), and incubated at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody solution (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) for 1 h at RT ...
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bioRxiv - Molecular Biology 2019Quote: T7-tagged cDNA template was PCR amplified and subjected to in vitro transcription using a MEGAscript T7 kit (Applied Biosystems). Amplified cRNA was purified with an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... pcDNA5/FRT/TO plasmid encoding Flag-tagged WT ExoU or S142A ExoU was incubated in serum free medium containing lipofectamine 2000 (Invitrogen) for 30 minutes at ambient temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were co-transfected with either a constant concentration of 0.1 μg of NF1-NanoLuc pcDNA expression plasmid or CRAF RBD-NanoLuc pcDNA expression plasmid with 0.2 μg of GFP-tagged KRAS (WT or Mutant) with 0.25 μl of Lipofectamine 2000 per well following the manufacturer’s protocol (Thermo Fisher Scientific). Twenty-four hours later ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with mCherry-tagged wild type or mutant ORP1L at 70% confluency using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The generation of HeLa stable cell lines expressing all different variants of FLAG-tagged HERPUD1 cloned in the pLVX-IRES-Puro vector were generated by transfection with Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... LLC-luc cells were stably transfected with CIITA overexpression vector pcDNA3-myc-tagged CIITA (addgene Plasmid #14650) or control pcDNA3.1 (Thermofisher V79020) using Lipofectamine 2000 Reagent (Invitrogen 11668-027) ...
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bioRxiv - Biophysics 2020Quote: ... C-terminal His6-tagged CLC-ec1 cloned in the pASK vector was transformed in E.coli BL21(DE3) (Invitrogen, Carlsbad, CA) and the expression of CLC-ec1 protein was induced with anhydrotetracycline (Acros Organics ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescently tagged G-actin was prepared by covalent modification with Alexa Fluor™ 594 Carboxylic Acid (Thermo Fisher Scientific 15461054) (Alvarado and Koenderink ...
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bioRxiv - Genetics 2022Quote: ZsGreen and flag-tagged ADAR1-p110 mRNA were made using the Invitrogen MEGAscript T7 Transcription Kit (Fisher Scientific, Cat #: AM1334) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cockayne syndrome B [CSB] fibroblast SV40 immortalized) and CS + CSB (CS cells stably expressing GFP tagged CSB from a BAC) cells were cultured in DMEM (ThermoFisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: Stable cell lines expressing low levels of HA-tagged Gli2 and Gli3 variants were generated using the Flp-In system according to the manufacturer’s recommendations (Life Technologies) (22) ...
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bioRxiv - Plant Biology 2021Quote: Expression vectors for HA-tagged PpCSLDs under control of their native promoters were constructed using Gateway Multisite Pro cloning (Invitrogen). The CSLD2 and CSLD6 coding sequences were amplified from cDNA clones pdp04669 and pdp21814 (RIKEN BioResource Center ...
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bioRxiv - Cell Biology 2022Quote: Histidine-tagged Mindin was purified from conditioned media collected from CHO-Mindin cells using Ni-NTA beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The resulting TGD057 HA-tagged DNA fragment was then cloned into the pTKO-att parasite expression vector (92) by Gateway Recombination Cloning Technology (Invitrogen). The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... the clarified lysates from His-tagged or dual tagged constructs were loaded onto an Nickel-nitrilotriacetic acid (Ni2+-NTA; 1.6 mL suspended beads/L growth) column (Thermo Scientific) equilibrated with 10 column volumes (CVs ...
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bioRxiv - Molecular Biology 2019Quote: ... 6X N-terminally Myc-tagged cDNAs encoding Ku70 or Ku80 WT and mutants were cloned into pcDNA/FRT/TO (Invitrogen) and then transfected along with Flp recombinase expressing pOG44 plasmid using Lipofectamine transfection reagent (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections were washed thoroughly and then incubated with appropriate secondary antibodies tagged with A lexaFluor-488 or -594 (1:500; Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Stably transfected HEK293 cells expressing N-terminally FLAG-tagged MOR or KOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Biophysics 2020Quote: ... HA or GFP-tagged DISP1 variants (anti-HA rabbit polyclonal, 1:1000 or anti-GFP rabbit polyclonal, 1:2000, Invitrogen), and β-actin (anti-β-actin rabbit polyclonal ...
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bioRxiv - Neuroscience 2022Quote: ... or isolated human synaptoneurosomes from AD brains (Tai et al., 2014; Tzioras et al., 2019) tagged with pHrodo Red succinimidyl ester (1:20, ThermoFisher) by media change ...
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bioRxiv - Genomics 2022Quote: A total of 6,114 animals from all year-classes evaluated in the sea lice challenges were pit-tagged and genotyped using custom ThermoFisher (Affymetrix) SNP arrays developed exclusively for AquaGen’s Atlantic salmon populations ...
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bioRxiv - Microbiology 2022Quote: ... for 1 hour at room temperature followed by additional 1 hour incubation with 1:200 dilution of fluorescence-tagged goat anti-mouse IgG Alexa Fluor 488 (Thermofisher) (Supplemental Table 5) ...
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bioRxiv - Neuroscience 2022Quote: ... we incubated them in secondary antibody solution (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated in Alexa Fluor 647-tagged donkey antirabbit IgG (1:500, Thermo Fisher, catalog # A-31573, RRID: AB_2536183) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no.A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Biochemistry 2024Quote: ... or expressing EGFP-tagged HRas V12/ HRas V12-C181S/ HRas V12-C184S were lysed in TRIZol at the end of the experiment (Invitrogen). RNA was isolated from the lysate by the chloroform-isopropanol method using the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... blocked with 10 % NDS-PB-TX and incubated in the dark in secondary fluorescently tagged anti-mouse antibody for either TH or GAD1 (1:200, MAB5406, EMD Milipore)) and fluorescently tagged anti-rabbit antibody for tracer (1:200, AlexaFluor, Invitrogen), overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: For expression analysis of truncated WDR47 constructs HEK cells were transfected with different HA tagged Wdr47 constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...