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Citations for Thermo Fisher :
5351 - 5400 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS and incubated in Alexa Fluor-647-tagged goat anti-mouse IgG (1:100; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... We generated a strain in which Dnm1 is tagged with mcherry and Mmm1 with GFP (MCY2199) in which mitochondria were labelled with Mitotracker (#M22426, Invitrogen). This setup allowed following and quantifying the presence of Dnm1-mCherry and Mmm1-GFP at sites of mitochondrial fission ...
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bioRxiv - Cell Biology 2024Quote: ... and transfected with a total of 2 μg C-terminal EGFP tagged M2 tail using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer protocol after washing with 200 μL PBS and refreshing with 100 μL DMEM (ScienCell ...
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bioRxiv - Biochemistry 2024Quote: ... or expressing EGFP-tagged HRas V12/ HRas V12-C181S/ HRas V12-C184S were lysed in TRIZol at the end of the experiment (Invitrogen). RNA was isolated from the lysate by the chloroform-isopropanol method using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The Fab from the CD4i antibody 17b37 was expressed by transient transfection using expression vectors encoding the LC and a C-terminally tagged HC portion of the Fab using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with goat anti-rat IgG (H+L) secondary antibody tagged with Alexa Fluor 488 (Invitrogen, A-21470). Tissue sections were washed and blocked with 1:100 mouse Fc block in 5% goat serum ...
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bioRxiv - Cell Biology 2023Quote: ... Y-A or YIY-AAA) and FLAG-tagged CDK-1 constructs were expressed in FreeStyle 293-F cells (ThermoFisher Scientific). Cells were transfected using FreeStyle MAX Reagent and OptiPRO SFM according to the manufacturer’s guidelines (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed three times for 10 min in 1X PBS and incubated for 4 h at 4°C with the fluorescently tagged secondary antibodies (Invitrogen; AlexaFluor 647 goat anti-mouse and AlexaFluor 488 donkey anti-rabbit ...
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bioRxiv - Molecular Biology 2023Quote: ... were transfected with expression vectors for 3xFLAG-tagged wt eIF3g or its mutated forms using Expifectamine 293 according to the manufacturer’s protocol (Thermo Scientific). Cells expressing 3xFLAG-tagged eIF3g (200 ml suspension ...
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bioRxiv - Cell Biology 2023Quote: Exon 1 carrying C-terminally tagged HttQ25-EmGFP and HttQ46-EmGFP were generated in the mammalian expression vector pTREX (Invitrogen). VCP (wt)-EGFP (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... Myc or FLAG-tagged procaspase-9 plasmid was prepared by cloning the procaspase-9 gene into a pcDNA-based vector (Invitrogen). Procaspase-9 with three mutations (Asp315Ala ...
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bioRxiv - Cell Biology 2022Quote: ... and 50 ng Myc-tagged GFP constructs were forward transfected into HFT cells seeded in a 6-well plate using Lipofectamine 3000 (Invitrogen). The cells were harvested 48 or 72 hours post transfection ...
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bioRxiv - Biochemistry 2023Quote: ... 155-238) were fused to the C-termini of tagged receptors and cloned into the NheI-XhoI sites of pCEP4 (Invitrogen). Plasmids for HIV-1 MN gp160 and BaL gp160 (# 100919 ...
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bioRxiv - Cell Biology 2023Quote: Lenti-X 293T cells were transfected with pCDNA3.1 plasmids expressing HA-tagged YWHA isoforms using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol and cultured for 48 h before lysis in 2% SDS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... or expressing EGFP-tagged HRas V12/ HRas V12-C181S/ HRas V12-C184S were lysed in TRIZol at the end of the experiment (Invitrogen). RNA was isolated from the lysate by the chloroform-isopropanol method using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells stably expressing N-terminally FLAG-tagged DOR or MOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 2.5 μg of GST-tagged RIT1 plasmids or GFP control plasmid using Lipofectamine 3000 Reagent (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells stably expressing N-terminally signal sequence FLAG (ssf)-tagged µOR (HEK293-µOR) were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Cell Biology 2023Quote: ... The ESCO1 cDNAs were cloned into a pcDNA5/FRT-based C-terminally Flag- or GFP-tagged vector and co-transfected with a plasmid expressing the FLP recombinase (pOG44, Invitrogen) using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Flp-In T-Rex-293 cells were co-transfected with the plasmid coding for the tagged LTP and the pOG44 plasmid encoding the Flp recombinase (Invitrogen). Positive clones were selected by adding 100 µg/ml hygromicin B and 15 µg/ml blasticidin on the day after transfection ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: Rescue experiments were conducted in LRBA-KO HEK293T and HaCat cell lines transfected with 2 μg of WT LRBA plasmid tagged with myc (Origen) using Lipofectamine 2000 (Invitrogen). These reconstituted cells lines are called Myc-LRBA in this manuscript ...
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bioRxiv - Molecular Biology 2024Quote: ... the mixture of 1.0 μg GFP-tagged TDP-43 CTFs and 1.0 μg RFP-tagged TDP-43 for two color co-expression) were transfected using Lipofectamine 2000 (Thermo Fisher). After 1 overnight incubation for transfection ...
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bioRxiv - Plant Biology 2024Quote: ... then cloned into the destination vector pEarleyGate101 with YFP-tagged sequences under the control of Pro35S using the LR recombination kit (Invitrogen) to obtain 35S::NPR1-YFP and 35S::TGA3-YFP constructs ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids for expression of SUMO-tagged efpL genes were generated with the Champion™ pET-SUMO Expression System from Invitrogen™ according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Randomisation was removed and samples were mass tagged using 10 channels of a TMT-11-plex stock (Thermo Fisher Scientific). For quantification control ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for human cells are mouse anti-human CD3 conjugated to APC (Thermo Fisher, 47-0036-42), mouse anti-human CD8 conjugated to FITC (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... where EGF and noggin were replaced with human forms with 10 ng ml−1 human EGF (Invitrogen, PHG0311), 100 ng ml−1 human noggin (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human TNF-α (hTNF-α)-PE and anti-human IFN-ɣ (hIFN-ɣ)-PECy7 were from ThermoFisher. Anti-human CD4 (hCD4)-BV711 was from Biolegend.
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... which contains the gene expression profile of human microglia using a human gene ST 2.0 Microarray Chip (Affymetrix). The human microglia were polarized to either pro-inflammatory (M1 ...
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bioRxiv - Immunology 2020Quote: ... using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] ...
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bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Immunology 2023Quote: Total human IgG levels were determined following the manufacturer’s instructions in the Human IgG Total ELISA Kit (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Synthetic Biology 2023Quote: ... primary human T cells were thawed and activated with anti-CD3/CD28 Human T-Expander Dynabeads (Thermo Fisher) at a 3:1 bead to cell ratio ...
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bioRxiv - Immunology 2024Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: All cell lines were cultured in DMEM media supplemented with sodium pyruvate and 10% Hi-FBS (Life Technologies). To express protein constructs in living cells ...
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bioRxiv - Genetics 2020Quote: ... Germany) 1 μl of each PCR product was edited with Hi-Di Formamide (20 μl, ThermoFisher, Massachusetts, USA) and Gene Scan 500 ROX dye Size Standard (0.5 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transient transfection with plasmids encoding Affimer-His constructs using Lipofectamine 2000 (ThermoFisher), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEL and K562 cells were all from American Type Culture Collection (ATCC) and cultured in RPMI with 10% HI FBS except for K562 (grown in IMDM (Gibco, #21980-032) with 10% HI FBS ...
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bioRxiv - Molecular Biology 2019Quote: Wild-type and indicated mutants of MmEsco1 were cloned into pEF6/Myc-His B vector (Thermo Fisher Scientific) containing C-terminal myc and His tags ...
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bioRxiv - Synthetic Biology 2021Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Briefly, sheep-anti mouse IgG paramagnetic beads (Dynal, 11201D) were coupled with anti-His mAb (Invitrogen, 37-2900) in coating buffer (PBS plus 0.1% immunoglobulin-free BSA ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were probed in PBST (PBS pH 7,4, 0,05% (v/v) Tween-20) with the mouse anti-his antibody (1:3000, Invitrogen) and the respective secondary antibody goat anti-mouse-HRP (1:3000 ...