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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ASCs were cultured in proliferation medium comprised of DMEM:Ham’s F12 supplemented with 10% fetal bovine serum (FBS) (Wisent, St. Bruno, Canada) and 100 U/mL penicillin/0.1 mg/mL streptomycin (1% pen-strep) (ThermoFisher, Waltham, USA) at 37°C with 5% CO2 and ~20% O2 ...
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bioRxiv - Developmental Biology 2021Quote: ... were cultured feeder-free on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix and maintained in Essential 8 Flex Basal Medium supplemented with Essential 8 Flex Supplement (50x) and 0.1% Pen/Strep (all from Thermo Fisher Scientific), at 37°C under normoxic conditions (21% O2 and 5% CO2) ...
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bioRxiv - Bioengineering 2020Quote: Fresh or frozen PBMCs were dispensed into 48-well plates at a density of 2 × 106 in 0.5 ml in RPMI-1640 supplemented with antibiotics Pen/Strep (TC medium; Life Technologies Ltd.) and 10 v/v human AB serum (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... followed by C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For SARS-CoV-2 RBD VOCs proteins ...
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bioRxiv - Immunology 2022Quote: ... followed by a foldon trimerization motif and C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For competition ELISA experiments ...
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bioRxiv - Genomics 2019Quote: ... rinsed with culture medium (IMDM medium supplemented with 10% FBS and 1% Pen/Strep) and then treated with 0.2 U/μL DNase I (Thermo Fisher Scientific) in 10 mL of culture medium at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal bovine serum (FBS; Hyclone, Cat# SH30396.03) and 1% penicillin-streptomycin (pen-strep; Thermo Fisher, Cat# 15140-122). PC3 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... (PCS-201-013) were purchased from ATCC and cultured in 10% Fetal Bovine Serum (FBS) and 1% pen-strep (Gibco, Life Technologies) in Dulbecco’s modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... were grown in Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 100 μg/ml penicillin-streptomycin (Pen-Strep) (Life Technologies). 293T cells were used for transient expression of the SARS-CoV-2 S gp variants ...
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bioRxiv - Genomics 2021Quote: ... (ATCC) supplemented with 10% FBS and 1% Pen/Strep) and then treated with 0.2 U/μL DNase I (Thermo Fisher Scientific) in 10mL of culture medium at 37°C for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... cells were cultured for 72 h in RPMI 1640 medium supplemented with 1% pen-strep and 5% exosome-depleted FBS (GibcoTM, ThermoFisher Scientific). sEV derived from A549 cells were isolated from the conditioned medium by differential ultracentrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... cortices from P3-P5 pups were micro-dissected into chilled Hibernate A (HibA) media (Hibernate A[Gibco], 1% Pen/Strep [Gibco]). Briefly ...
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bioRxiv - Systems Biology 2023Quote: MCF-7 cells transfected with p53-mVenus and H2B-Cerulean were maintained in RPMI-1640 media supplemented with 10% (v/v) FBS and 1% (v/v) Pen-Strep (all reagents from Life Technologies) in a humidified incubator at 37 °C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: For sEVs isolation cells were cultured for 72 h in RPMI 1640 medium supplemented with 1% pen-strep and 5% exosome-depleted FBS (GibcoTM, ThermoFisher Scientific). sEV were isolated from the conditioned medium (CM ...
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bioRxiv - Molecular Biology 2022Quote: ... cells maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS and Pen-Strep (100 U / ml penicillin, 100 mg / ml streptomycin, Gibco 15140-122) at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: Hindbrain regions of st.18 HH embryos were dissected in sterile PBS with Penicillin-Streptomycin (Pen-Strep, 1:100; Gibco, USA), then placed in a tube containing human embryonic stem cell medium (hESC ...
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bioRxiv - Neuroscience 2023Quote: ... dermis and epidermis were mechanically separated and the dermal part was collected in fibroblast cultivation medium (DMEM/F12 + 100 U/ml penicillin 100 µg/ml streptomycin [pen/strep; both: Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The cells were resuspended in 3mL of warm complete RPMI media (Gibco RPMI #14175, 10% FBS, 1x Pen/Strep (Gibco #15140)) and counted and viability recorded using trypan-blue exclusion on an automated cell-counter (Countess™ ...
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bioRxiv - Cell Biology 2024Quote: ... 20 mL of fresh DMEM media (supplemented with FBS and Pen/Strep) containing 10% SPIONs particles and 10 mM HEPES pH 7.4 (Thermo Fisher, 15630080) was added ...
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Proto-sex locus in large yellow croaker provides insights into early evolution of the sex chromosomebioRxiv - Evolutionary Biology 2020Quote: ... The biotinylated probes specifically attached to the slides were detected by avidin-Alexa Flour-488 (Invitrogen, CA, USA), and chromosomes were counter-stained with propidium iodide in anti-fade solution ...
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bioRxiv - Evolutionary Biology 2021Quote: ... biotinylated baits were bound to streptavidin-coated magnetic beads (Dynabeads MyOne Streptavidin; Thermo Fisher Scientific, Waltham, MA, USA), followed by stringent washing to remove unbound DNA library molecules ...
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bioRxiv - Immunology 2020Quote: ... Tetramers were prepared in house by mixing biotinylated (NANP)9 peptide with streptavidin conjugated PE or APC (Invitrogen) in a 4:1 molar ratio ...
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bioRxiv - Immunology 2021Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated ovalbumin was conjugated to streptavidin-BV711 (BD biosciences ...
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bioRxiv - Biophysics 2020Quote: ... and then incubated with biotinylated Epidermal Growth Factor (EGF) complexed to Texas Red™ Streptavidin (ThermoFischer/Molecular Probes Cat ...
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bioRxiv - Immunology 2021Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated ovalbumin was conjugated to streptavidin-BV711 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated ovalbumin was conjugated to streptavidin-BV711 for single-cell sorts (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated ovalbumin was conjugated to streptavidin-BV711 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated and isolated from free biotin using EZ-Link™ NHS-LC-LC-Biotin (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed with cold PBS and biotinylated with 0.5 mg/ml sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate (ThermoFisher) while gently shaking for 45 mins on ice ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated ovalbumin was conjugated to streptavidin-BB515 (BD ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the product was biotinylated using the Pierce RNA 3’ End Desthiobiotinylation Kit (Thermo Fisher Scientific, Waltham, MA). Biotinylated lncov1 fragments were linked to spherical beads of the Pierce Magnetic RNA-Protein Pull-Down Kit (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... a hybridization cocktail of biotinylated target was incubated with a GeneChip® Mouse Clariom S array (ThermoFisher Scientific) at 60rpm for 16hours at 45°C in a Genechip® Hybridisation Oven 645 (Affymetrix) ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated cRNA was generated and fragmented according to the Affymetrix protocol and hybridized to 430 mouse microarrays (Affymetrix). As described in Trageser et al (44) ...
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bioRxiv - Microbiology 2022Quote: Purified F antigens were biotinylated using an EZ-link Sulfo-NHS -LC-Biotinylation kit (Thermo Fisher, cat#A39257) with a 1:1.3 molar ratio of biotin to F ...
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bioRxiv - Cancer Biology 2022Quote: ... biotinylated PCR product is bound and isolated using Dynabeads M-280 Streptavidin magnetic beads (Thermo Fisher Scientific, #11206D). Briefly ...
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bioRxiv - Genomics 2022Quote: ... and a 1:100 dilution of Anti-mouse CD34 biotinylated antibody (Thermo Fisher Scientific, Cat#: 13-0341-85) for 20min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... fibronectin was biotinylated by incubating 0.1mg/mL fibronectin with 0.5mM EZ-Link Sulfo-NHS-LC-Biotin (A39257, ThermoFisher) for 30 min at RT ...
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bioRxiv - Biochemistry 2019Quote: ... Biotinylated template RNA was incubated with 250 ng streptavidin or 50 μl streptavidin-agarose resin (Thermo Fisher Scientific). Reactions were stopped with 10 mM EDTA ...
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bioRxiv - Molecular Biology 2020Quote: ... EdU-genomic DNA was then biotinylated using the Click reaction and purified using Streptavidin T1 magnetic beads (Invitrogen). Libraries for Illumina sequencing were constructed using TruSeq ChIP Library Preparation Kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of the biotinylated probe was done using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher 89880).
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... plates were washed and incubated with 50 μl of 0.5 μg/ml biotinylated-porcine IFN-γ antibody (Invitrogen) for 2h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... were biotinylated using the EZ-Link Sulfo-NHS-LC-biotinylation kit according to manufacturer instructions (Thermo Fisher Scientific) and used in the assay at concentrations of 1.25 ...
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bioRxiv - Cell Biology 2019Quote: ... 50 pmol of in vitro transcribed RNA was biotinylated using RNA 3’ End biotinylation Kit (Thermo Fisher Scientific) overnight at 16°C ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... rshACE2 and deglyco-rshACE2) were biotinylated using the EZ-Link Sulfo-NHS-LC-Biotin kit (Thermo Fisher Scientific). Excess sulfo-NHS-LC-biotin was quenched by adding Tris-HCl buffer (800 mM ...
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bioRxiv - Immunology 2021Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated ovalbumin was conjugated to streptavidin-BV711 (BD biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated hACE2-Fc was prepared using EZ-Link™ Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated Ovalbumin was conjugated to streptavidin-BV711 (BD biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with primary antibodies and subsequently with the corresponding biotinylated secondary antibodies (1:1000 Goat anti-rabbit, Invitrogen). Signal amplification was performed using the ABC complex (Vectashield) ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated using the EZ-Link Sulfo-NHS-LC-Biotinylation kit according to the manufacturer’s instructions (Thermo Scientific). Biotinylated ovalbumin was conjugated to streptavidin-BV711 for single-cell sorts (BD biosciences ...
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bioRxiv - Microbiology 2022Quote: ... biotinylated SARS-CoV-2 Spike trimer (Hexapro) was incubated with red streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2h at 37°C ...