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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Real-time qPCR for measuring mRNA levels was performed using Step-One-Plus real-time PCR System (Applied Biosystems) and the Fast SYBR Green Master mix (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein concentrations were determined by measuring UV absorbance at 280 nm with calculated molecular weights and extinction coefficients based on ProtParam EXPASY tool (https://web.expasy.org/protparam/) using a NanoDrop One spectrophotometer (Thermo Fisher). Aliquots of purified protein in 1x PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR with diluted cDNA was performed in duplicate for all tested genes with the Step One system (Applied Biosystems) and Fast SYBR™ Green Master Mix (4385612 ...
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bioRxiv - Physiology 2024Quote: ... Real-time PCR was performed with a Step One Plus Detection System (Real-Time PCR System-Thermo Fisher Scientific). The results represent the average from three technical replicates for each analyzed animal ...
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bioRxiv - Microbiology 2024Quote: The concentration of the DNase treated RNA was determined by measuring the A260/A280 ratio (Nanodrop One, Thermo Scientific). A 25 μl reaction was prepared for each sample using the following TaqMan reverse transcription reagents ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We homogenized blades with one 2.8 mm ceramic bead using a bead mill (BeadMill24, Fisher Scientific, Waltham, MA, USA) at a speed of 4 m/sec for 45 seconds until each blade was homogenized into a fine powder ...
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bioRxiv - Cell Biology 2024Quote: Samples were measured with the Evosep One LC system (EvoSep) coupled to an Orbitrap Astral mass spectrometer (Thermo Fisher). Peptides were separated on an Aurora Elite column (15 cm x 75 mm ID with 1.7 mm media ...
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bioRxiv - Cell Biology 2024Quote: Samples were measured with the Evosep One LC system (EvoSep) coupled to an Orbitrap Astral mass spectrometer (Thermo Fisher). Peptides were separated on an Aurora Elite column (15 cm x 75 mm ID with 1.7 mm media ...
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bioRxiv - Developmental Biology 2024Quote: ... The signals were detected using Chemi-Lumi One L (Nacalai) or Pierce Western Blotting Substrate Plus (Thermo Fisher Scientific). The following primary antibodies were used ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular DNA was extracted with one volume of phenol:chloroform:isoamyl alcohol (25:24:1, #15593031, ThermoFisher Scientific, Waltham, MA, USA), precipitated with one volume of isopropyl alcohol ...
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bioRxiv - Plant Biology 2024Quote: ... DNA quantity and quality were checked using a NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA). Gibson assembly was performed according to the manufacturer’s instructions using NEBuilder® HiFi DNA Assembly (New England BioLabs Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... After the RNA quality and quantity was assessed with a NanoDrop™ One (Thermo Fisher Scientific, Waltham, MA, USA), the samples were stored at −80 ◦C.
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bioRxiv - Neuroscience 2024Quote: A duplex real-time PCR (ABI Prism Step One real-time PCR apparatus, Thermo Fisher Scientific, Waltham, MA, USA) was performed in triplicates of 1:10 diluted cDNA samples with predesigned FAM-labeled TaqMan® assays (Applied biosystems by Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... The yields of the total RNA for each sample were determined using a Nanodrop one spectrophotometer (Thermo Fisher Scientific). 100 ng total RNA and oligo(dT)20 primer were used for reverse transcription by SuperScript™ III First-Strand Synthesis System (ThermoFisher Scientific #18080-051 ...
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bioRxiv - Molecular Biology 2024Quote: ... one or two copies of the UGI domain were amplified by Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific) and cloned into designated positions ...
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bioRxiv - Biochemistry 2024Quote: ... The mass concentration of purified total mRNA was determined using the RNA concentration function on a NanoDrop One (ThermoFisher). The mass concentration was then converted to a molar concentration using an online tool “Weight to Molar Quantity (for nucleic acids ...
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bioRxiv - Cell Biology 2024Quote: ... we cloned the zact sequence into the bacterial plasmid pCR 2.1 (TOPO TA cloning kit with One Shot TOP10 chemically competent cells, Invitrogen). TOP10 chemically competent cells were also used in other steps.
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR was conducted on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using 12 ng cDNA per reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 hours prior to collection organoids were incubated with 1 μM EdU (Thermo fisher, #C10636).
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bioRxiv - Cell Biology 2020Quote: ... RT-PCR was performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) with LightCycler 480 SYBR Green I Master (Roche) ...
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Immunology 2021Quote: ... vRNA was quantified using the QuantStudio 6 Real-Time PCR System (Applied Biosystems, Waltham, MA). Five microliters vRNA was added in duplicate to a 0.1 mL 96-well MicroAmp fast optical reaction plate (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were seeded at equal densities into wells of 6-well plates (Nunc, Roskilde, Denmark). The cells were pre-treated with EVs for 24 h and then stimulated with LPS (LL24 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was quantified using Qubit high sensitivity assays (Thermo Fisher Scientific)6-10 μg of DNA was digested with 120 Units Csp6I enzyme (Thermo Fisher Scientific) [3-5 Csp6I digests per sample] overnight at 37°C in thermomixer shaking at 900 rpm (30s on ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were run on a QuantStudioTM 6 Flex Real-Time PCR System (ThermoFisher; Cat #: 4485691). Fold change calculations were done based on the ΔΔCt method ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.05 % xylene cyanol) and the products were separated on a 6 % urea-PAGE gels (Invitrogen) run at 180 V for 50 min ...
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bioRxiv - Neuroscience 2022Quote: ... We plated an amount of 6×105 primary mouse cortical neurons (ref. Gibco A15586, ThermoFisher) on each coated coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... We plated an amount of 6×105 primary mouse cortical neurons (ref. Gibco A15586, ThermoFisher) on each coated coverslip ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed in the QuantStudio 6 Flex Real-Time System (Applied Biosystems, Waltham, MA). Target gene expression levels were determined by the comparative 2∧-(delta C(T) ...
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bioRxiv - Neuroscience 2021Quote: ... and 5% (by volume) fluorescent bead solution (6 μm FocalCheck Microspheres; part no. F14807; ThermoFisher), prepared using deionized water ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) was performed using the Quantstudio 6 Flex (Applied Biosystems, Life Technologies, USA). For the qPCR reaction 1 μl of 1:20 diluted cDNA in MilliQ was used with 1 μl primer mix (forward and reverse primers ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixtures were run on a QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific) with combined annealing and elongation cycles (Step1 ...
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bioRxiv - Molecular Biology 2019Quote: ... 6’-Diamidino-2-phenylindole (DAPI; Cat#D1306; RRID: AB_2629482) were purchased from Invitrogen (Carlsbad, CA). Neurobasal® medium ...
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bioRxiv - Genomics 2020Quote: ... transduced cells were seeded into 6-well format in 1.5 μg/mL puromycin (Life Technologies) containing medium ...
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bioRxiv - Genomics 2020Quote: ... plated in 6-well plates then stimulated with anti-CD3/anti-CD28 Dynabeads (Life Technologies) over a period of 24-hours ...
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bioRxiv - Physiology 2019Quote: Total RNA was isolated from 6 adult female medaka (WT) pituitaries using Trizol (Ambion, USA) and cDNA was prepared from 30 ng total RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR assays were performed on either QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) or CFX384 Touch Real-Time PCR Detection System (BIO-RAD) ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Cancer Biology 2019Quote: ... then resuspended in the FACS buffer with 6 µg/mL Hoechst 33258 (Thermo Fisher; H3569) and either 2-µm YG microspheres (Polysciences ...
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bioRxiv - Synthetic Biology 2019Quote: Samples were resolved by electrophoresis in 6% or 15% polyacrylamide TBE-Urea gels (Thermo Fisher) in TBE ...
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bioRxiv - Synthetic Biology 2019Quote: ... Im7-6 was purified from IVG reactions using magnetic His-tag Dynabeads (Thermo Fisher Scientific). The IVG reactions were diluted in 90 µl of Buffer 1 (50 mM NaH2PO4 and 300 mM NaCl ...
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bioRxiv - Genetics 2021Quote: ... The PCR product was then loaded on a Novex 6% TBE-Urea gel (Life Technologies) and a band around 160-170 bp was excised from the gel ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then labeled using 6-plex tandem mass tags (TMT; Thermo Scientific, Waltham MA) according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then passaged 1:3-1:6 every 2-3 days using Accutase (Gibco).
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bioRxiv - Bioengineering 2021Quote: ... 6-well well-plates were first coated with vitronectin (5 μg/mL, Thermo Fisher, USA) followed by a coating with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with DAPI (4ʹ,6-diamidino-2-phenylindol) 1:10,000 (Thermo Fisher Scientific). Images were recorded using an Axioimager 2 Microscope with cooled CCD camera (SPOT Imaging Solutions ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded at a density of 6 × 104 cells/cm2 in Neurobasal medium (ThermoFisher), supplemented with B-27 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lines were transfected in 6-well plates using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... which were purified by band excision after polyacrylamide denaturing gel electrophoresis (Novex 6% TBE, Invitrogen). Quantification of libraries was performed by qPCR (KAPA SYBR ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR were performed on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) using 5 μL Power SYBR Green PCR Mix (Applied Biosystems) ...