Labshake search
Citations for Thermo Fisher :
5101 - 5150 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2019Quote: ... One microliter of RT-RamDA mix (2.5× PrimeScript Buffer, 0.6 pmol oligo(dT)18 (Cat# SO131, Thermo Fisher Scientific), 8 pmol 1st-NSRs [30] ...
-
bioRxiv - Molecular Biology 2019Quote: ... qPCR was performed using the comparative CT method and a Step-One-Plus Real-Time PCR Instrument (Applied Biosystems). SYBR Green reactions were carried out in technical duplicate or triplicate in a final volume of 10 μl ...
-
bioRxiv - Biochemistry 2020Quote: ... The absorbance of the samples was collected at 620 nM using a NanoDrop One UV– Vis Spectrophotometer (Thermo Scientific) and the concentration of monophosphate determined using a standard curve generated from purified monophosphate.
-
bioRxiv - Biochemistry 2020Quote: ... All protein concentrations were determined by absorbance at 280 nM using a NanoDrop One UV–Vis Spectrophotometer (Thermo Scientific).
-
bioRxiv - Biochemistry 2020Quote: ... the protein concentration was determined by measuring the A280 using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, MA, USA). This protein was applied to a 16/600 Superdex 75 preparative size exclusion column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by sequencing via SuperScript III One-Step RT-PCR System with Platinum Taq RT-PCR (Life Technologies, USA) conducted by Life Technologies Biotechnology (Shanghai ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... for one hour at 37°C and then purified using the MagJET NGS Cleanup Kit (Thermo Fisher Scientific, USA). 50 ng of the insert and 50 ng of the backbone (molar ratio of ∼1:10 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... We used SensiFAST Probe No-ROX One-Step Kit (Meridian Bioscience) and PikoReal Real-Time PCR System (Thermo Scientific) with following cycling conditions ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The obtained RNA was reversed-transcribed and PCR-amplified using the Superscript III One Step RT-PCR system (Invitrogen) followed by an additional error-prone PCR step (20 cycles ...
-
bioRxiv - Immunology 2020Quote: ... PCR was performed to determine RNA copies/mL using the Superscript III Platinum One-Step qRT-PCR system (Invitrogen). Standard curves were obtained by using a quantitative PCR (qPCR ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The quality and quantity of total RNA were determined with a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher). cDNA was synthesized using iScript cDNA synthesis kit (Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2020Quote: ... Viral RNA was extracted using easyMAG (bioMérieux) and one step Reverse-Transcription-PCR was performed with Superscript III (Invitrogen), Platinum Taq HiFi Polymerase (Thermo Fisher ...
-
bioRxiv - Microbiology 2020Quote: ... The RT-qPCR reaction was prepared using the AgPath-ID-One-Step RT-PCR kit (Thermo Fisher Scientific, USA) adjusted to a volume of 12.5 μl [19] ...
-
bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using a Superscript III one-step RT-PCR system with platinum Taq DNA polymerase (Invitrogen) with four sets of specific primers ...
-
bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using a Superscript III one-step RT-PCR system with platinum Taq DNA polymerase (Invitrogen) with primers covering the entire S segment region according to a previously reported study [9] ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Nucleic acids concentration was measured by absorbance at 260 nm using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, USA). All selections were performed using SELEX buffer (100 mM NaCl ...
-
bioRxiv - Immunology 2020Quote: ... PCR was performed using the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (ThermoFisher Scientific) as per manufacturer’s instructions with the addition of 2 μl MgSO4 ...
-
bioRxiv - Molecular Biology 2019Quote: ... RT-PCRs were performed using the One-step Superscript III RT-PCR kit with Platinum Taq polymerase (Life Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... One milliliter of lysate was pre-absorbed with 40 µl of Pierce Protein A Plus Agarose (Thermo Fisher Scientific) for 30 min at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... BraunMelsungen AG) which in turn was connected to one end of a PTFE microtube (0.56 × 1.07 mm, Fisher Scientific). The tubing was filled with the culture and the terminal end of the tubing connected to the inlet of a microfluidic device (channel cross section ...
-
bioRxiv - Cell Biology 2019Quote: ... 7.25 µl of 0.2% gelatin (w/v) was added and the mixture was printed on one-chamber Lab-Tek slides (Nunc) with a contact printer (BioRad ...
-
bioRxiv - Genetics 2020Quote: ... One microgram of purified RNA was converted into cDNA using Superscript™ III First-Strand Synthesis System (Invitrogen, #18080051) with random hexamer primers ...
-
bioRxiv - Cell Biology 2021Quote: ... The color reaction was started by adding 100 μL of One-step Ultra TMB ELISA substrate (Thermo Scientific #34028) was added to each well (100 μL) ...
-
bioRxiv - Cell Biology 2021Quote: ... Quantitative PCR was performed in a 96-well plate using the SYBR green master mix using AB7300 Real-Time PCR thermal cycler with Step One software (v2.2.2; Applied Biosystems). Hprt and Pgk1 were used as housekeeping genes to normalize the expression ...
-
bioRxiv - Neuroscience 2021Quote: ... with the remaining one third for RNA-seq was mixed with 100 U SUPERase-In RNAse inhibitor (ThermoFisher, AM2694) and frozen at −80 °C for downstream RNA isolation.
-
bioRxiv - Physiology 2021Quote: ... One microgram total RNA was used for reverse transcriptase (RT)-mediated synthesis of cDNA using Superscript II RT (Invitrogen) and random hexamer primers for Cyp7a and Superscript IV (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... and AMG44 (final concentration: 5μM) treatment one-hour after plating in cell media (DMEM/F12 [Gibco, 10565018], 1% N2 [Gibco, 17502048] ...
-
bioRxiv - Neuroscience 2020Quote: ... Dissociated cells received diluted 4-PBA (final concentration: 10mM) and AMG44 (final concentration: 5μM) treatment one-hour after plating in cell media (DMEM/F12 [Gibco, 10565018] ...
-
bioRxiv - Cell Biology 2021Quote: Plasmids encoding full length SARS-CoV-2 S (GenBank ID: QHD43416.1) with one or more mutation sites were carried into HEK-293T cells using lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instruction ...
-
bioRxiv - Pathology 2021Quote: ... Gene expression was quantified by real-time PCR using the Step One Plus Detection system (Applied Biosystems, CA, USA). TaqMan probes for mouse Ptch1 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were cultured for one week until confluent in MEM for SILAC (Thermo) supplemented with 10% dialyzed FBS (Gibco), L-glutamine ...
-
bioRxiv - Cell Biology 2020Quote: ... and 1X PBS) before blocking for one hour with blocking buffer (Bovine Serum Albumin (Fisher Scientific, Fair Lawn, NJ), Normal Donkey Serum (Jackson ImmunoResearch Inc. ...
-
bioRxiv - Genetics 2021Quote: ... One microgram of total RNA was used to synthesize cDNA using the SuperScript IV VILO Master Mix (Thermofisher Scientific).
-
bioRxiv - Genomics 2019Quote: ... PCR reactions were performed using MEL_015 and MEL_016 specific primers and one unit of Phusion DNA Polymerase (Thermo Scientific) in a 50 μl final volume.
-
bioRxiv - Immunology 2020Quote: ... flat-bottom 96-well plates containing feeder cells that had been seeded at a density of 28,600 cells/well one day prior in 100 μL of IMDM media (Gibco) containing 10% fetal calf serum ...
-
bioRxiv - Microbiology 2020Quote: ... The RT-PCR was performed using the SuperScript III Platinum One-Step Quantitative RT-PCR system (Invitrogen, Carlsbad. CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
-
bioRxiv - Microbiology 2020Quote: ... We quantified the yield and the quality of the DNA extracted using a NanoDrop One spectrophotometer (Thermo Scientific; USA) and a Qubit (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by real-time RT-qPCR (EXPRESS One-Step Superscript™ qRT-PCR Kit, universal Invitrogen using 3.5µL of RNA and 6.5µL of RT qPCR mix and standard fast cycling parameters ...
-
bioRxiv - Microbiology 2020Quote: ... was suspended in 600 μL of Qiagen RLT lysis buffer supplemented with one percent beta-mercaptoethanol and 0.3 U/μL Superase-In (Invitrogen). The suspension was subjected to bead beating for 3 minutes using 1.0 mm Zirconia/Silica beads ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and reverse-transcribed one microgram of RNA from each sample with Oligo (dT)18 (100μM) (Thermo Scientific, Life Technologies) and RevertAid H minus RT reverse transcriptase (Thermo Scientific ...
-
bioRxiv - Immunology 2021Quote: ... The S protein gene cDNA was amplified with a SuperScript IV One-Step RT-PCR kit (Thermo Fisher Scientific) using primers flanking the S gene ...
-
bioRxiv - Immunology 2020Quote: ... qPCR reactions were run on a Step one Plus realtime PCR apparatus using the absolute quantification program (Applied Biosystems), with the following conditions ...
-
bioRxiv - Molecular Biology 2021Quote: Cells were lysed by adding one volume of TNES buffer (TNE + 1% SDS) supplemented with RNaseA (Invitrogen cat #12091021) at final concentration of 100 μg/mL ...
-
bioRxiv - Microbiology 2021Quote: ... This was incubated with periodic agitation for one hour at 37°C before being aspirated and replaced with 0.8% molten agar in DMEM/F-12 (Gibco) and 1.5 μg / mL TPCK trypsin ...
-
bioRxiv - Immunology 2020Quote: ... and Covid-19 virus detection was performed using One-Step qRT-PCR SuperScript™ III Platinum™ (Life Technologies) targeting the gene E ...
-
bioRxiv - Microbiology 2021Quote: One microgram of DNA-free RNA was reverse transcribed using random hexamers and Superscript III Reverse Transcriptase (RT) (Invitrogen) following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2021Quote: ... The quantity and the quality of obtained DNA were assessed using a NanoDrop ONE spectrophotometer (Thermo Fisher Scientific, USA).
-
bioRxiv - Genetics 2019Quote: ... reverse-transcribed according to the manufacturer’s protocol (SuperScriptIII One-Step RT-PCR system with Platinum Taq DNA Polymerase;Invitrogen, Cat# 12574-018 ...
-
bioRxiv - Immunology 2021Quote: ... one time with PBS and final wash with water before mounting with ProLong Gold Antifade Mountant with DAPI (ThermoFisher).
-
bioRxiv - Microbiology 2020Quote: ... Bacterial pellets were suspended in 600 μL Qiagen RLT lysis buffer supplemented with one percent beta-mercaptoethanol and 0.3 U/μL Superase-In (Invitrogen). The suspension was subjected to bead beating for 3 minutes using 1.0 mm Zirconia/Silica beads ...