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Citations for Thermo Fisher :
5301 - 5350 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Hepa1-6 and HEK293A cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, #11995) supplemented with 10% heat inactivated FBS and 50 U/ml penicillin-streptomycin (PenStrep ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR was conducted on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using 12 ng cDNA per reaction ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was prepared from dissected cortex of P30 C57Bl/6 mice using Trizol (Invitrogen) and reverse-transcribed by Quantiscript Reverse Transcriptase (Qiagen) ...
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bioRxiv - Biophysics 2020Quote: ... 0.2-0.5 pmol of the reaction products were loaded on a 6% agarose gel (Invitrogen) and run for 90 mins at 90 V with 0.25x Tris-Borate as running buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... the cDNA was analyzed on a QuantStudio 6 Flex Real-Time PCR system (ThermoFisher Scientific). Fold changes were calculated using the ΔΔCq method ...
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bioRxiv - Microbiology 2020Quote: ... hPSCs were cultured on Matrigel-coated 6-well plates in commercial StemFlex™ Medium (ThermoFisher). The medium was changed every day ...
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bioRxiv - Immunology 2020Quote: CRP and IL-6 were measured using a high sensitivity ELISA (Life Technologies, Carlsbad CA). Plasma soluble CD14 (sCD14 ...
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bioRxiv - Immunology 2020Quote: Cell culture supernatant (diluted ¼) was subjected to ELISA (TNFα, Thermo Scientific, Waltham, MA, n=6) and Luminex (Cytokine Human Magnetic 30 Plex Panel ...
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bioRxiv - Immunology 2020Quote: ... Cells were maintained at 37°C and 6% CO2 FreeStyle 293 Expression Medium (Thermo Fisher) and 0.2% Penicillin/Streptomycin 7 days post transfection ...
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bioRxiv - Genetics 2020Quote: ... EBs were plated on laminin (Cat# 23017015) coated 6-well plates (Nunc; Thermo Fisher Scientific) on day 7 ...
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bioRxiv - Genetics 2020Quote: ... EBs were plated on laminin (Cat# 23017015) coated 6-well plates (Nunc; Thermo Fisher Scientific) on day 7 ...
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bioRxiv - Genomics 2021Quote: ... In step 6 we used Phusion Flash High-Fidelity PCR Master Mix (Thermo Fisher Scientific) for 18 cycles of PCR ...
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bioRxiv - Cell Biology 2021Quote: ... 12-well or 6-well Nunclon™ Delta Surface multi well plates (Thermo Fisher Scientific). For live-cell imaging ...
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bioRxiv - Cell Biology 2021Quote: ... expanded in E8 on geltrex-coated 6 well plates and routinely passaged using Versene (Gibco). Pluripotency of the isolated iPSC lines was confirmed by immunofluorescence (IF) ...
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bioRxiv - Immunology 2021Quote: ... Fluorescence signals were detected with a QuantStudio 6 Sequence Detector (Applied Biosystems, Foster City, CA). Data were captured and analyzed with Sequence Detector Software version 1.3 (Applied Biosystems).
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) was performed using the Quantstudio 6 Flex (Applied Biosystems, Life Technologies, USA). For the qPCR reaction 1 μl of 1:20 diluted cDNA in MilliQ was used with 1 μl primer mix (forward and reverse primers ...
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was loaded onto a Ni affinity column (Ni-Sepharose 6 FF, Invitrogen). The His-tag was cleaved by the HRV3C protease on the column ...
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was loaded onto a Ni affinity column (Ni-sepharose 6 FF, Invitrogen). GreA was eluted with the lysis buffer supplemented with 500 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... qPCR was performed on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) with steps of 10 min at 95 °C and 40 cycles of 15 s at 95 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... incubated in 6 ml of HBSS containing 0.15 mg/ml Collagenase I (Invitrogen, 17100-017) at 37°C for 35 min ...
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bioRxiv - Cell Biology 2022Quote: ... Suspension cells (THP-1) were first seeded for 6 h on poly-D-lysine (Gibco)-coated wells before starting the assay ...
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bioRxiv - Immunology 2022Quote: We labeled MC38/gp100 and NALM-6 cells with Cell-Trace Violet (Invitrogen cat. # C34557) dye at a concentration of 5 μM ...
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bioRxiv - Immunology 2022Quote: Caco2 cells were grown in 6-wells and 12-wells cluster plates (Thermo Scientific Nunc Cell-Culture Treated ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Each reaction consisted of 6 μl of PowerUp™ SYBR™ Green Master Mix (ThermoFisher), 0.5 μl of each primer solution at 3.6 μm and 5 μl of diluted DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Grids were blotted for 2 - 6 s in a Vitrobot (Mark IV, Thermo Fisher Scientific) at 4 °C and 100% humidity ...
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bioRxiv - Systems Biology 2022Quote: ... with the addition of 6 μl ERCC Spike-In Control Mix 1 (Ambion Thermo Fisher) diluted 1:10,000 ...
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bioRxiv - Systems Biology 2022Quote: ... with the addition of 6 μl ERCC Spike-In Control Mix 1 (Ambion Thermo Fisher) diluted 1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative real-time PCR was performed with the QuantStudio 6 Flex (Applied Biosystems, Life Technologies) using SYBR qPCR master mix (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... each with an N-terminal (His)6 tag from plasmids generated by GeneArt (ThermoFisher Scientific). For each purification ...
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bioRxiv - Immunology 2023Quote: ... Using Polycarbonate Cell Culture Inserts (0.4 µm pore) in a 6-well plate (Nunc, 140660), 50’000 Ge518_PTPRZ1-KO cells were seeded in the bottom of the well and 50’000 Ge518_PTPRZ1-KI cells were seeded at the top of the insert ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl cDNA (1000 ng/ 100 μl) and 6 μl nuclease-free water (Thermo Scientific). qRT-PCR cycles included pre-incubation at 95°C for 5 minutes followed by 40 cycles of 95°C for 10 seconds 60°C for 30 seconds and 72°C for 30 seconds ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed 6 times as before and mounted using Prolong Gold Antifade (Thermo Fisher) for confocal microscopy or maintained in PBS at 4°C prior to STORM imaging ...
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bioRxiv - Genomics 2022Quote: ... Real-time PCR was performed on a QuantStudio 6 Flex real-time thermocycler (Applied Biosystems) with the following thermocycling conditions ...
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bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected in 6 well plates at 60% confluence using lipofectamine LTX (Invitrogen) with 1µg of each transposon vector along with 0.5µg of pCMV-m7PB hyperactive transposase (34) ...
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bioRxiv - Molecular Biology 2022Quote: ... The transfection mix was replaced after 6 hours by 293 SFM II suspension medium (Invitrogen, 11686029 ...
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bioRxiv - Biochemistry 2022Quote: ... was used for quantifying the specific transcripts and analyzed with QuantStudio 6 Flex (Life Technologies). Primers used in qPCR analysis are mentioned in sup ...
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bioRxiv - Microbiology 2022Quote: ... and quantified mRNA levels with the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). For each high-ranked and low-ranked HB candidate gene ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded onto a Ni affinity column (Ni-Sepharose 6 FF, Invitrogen). The His-tag was cleaved by the HRV3C protease on the column ...
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bioRxiv - Bioengineering 2024Quote: ... and Mycoplasma testing within 6 months with the MycoStrip Mycoplasma Detection Kit (Invitrogen, Waltham, MA). Cell lines were maintained in culture at 37°C in 5% CO2.
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bioRxiv - Bioengineering 2024Quote: ... and Mycoplasma testing within 6 months using the MycoStrip Mycoplasma Detection Kit (Invitrogen, Waltham, MA). Cell lines were maintained in culture at 37°C in 5% CO2.
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR was performed using the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using SYBR® Green master mix (Applied Biosystems) ...
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bioRxiv - Genetics 2024Quote: ... RT-qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) with Fast SYBR Green Master mix (Applied Biosystems) ...
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bioRxiv - Genomics 2024Quote: ... with 10ng cDNA per reaction on a QuantStudio 6 Real-Time PCR system (Applied Biosystems). All RT-qPCR reactions were performed in triplicate and outliers were removed if the Ct value measured differed more than 0.5% from the mean ...
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bioRxiv - Genomics 2024Quote: ... 1.2 x 106 cells were transfected in 6-well plates using Lipofectamine 3000 (Invitrogen, L3000008) according to the manufacturers instructions with 200 ng pMD2.G (Addgene #12259) ...
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bioRxiv - Genomics 2024Quote: ... with 10ng cDNA per reaction on a QuantStudio 6 Real-Time PCR system (Applied Biosystems). All RT-qPCR reactions were performed in triplicate and outliers were removed if the Ct value measured differed more than 0.5% from the mean ...
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bioRxiv - Immunology 2024Quote: ... 4346906) with a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, 4485691). Quantification of gene expression was calculated as described by Pfaffl et al ...
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bioRxiv - Cell Biology 2023Quote: ... 6 × 105 cells/ml were plated in a 90 mm petri dish (101R20, Thermo Scientific). Cells were treated with LLOMe for 30 minutes at a concentration of 1 mM by dilution in RPMI medium ...
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bioRxiv - Neuroscience 2022Quote: ... A nuclear counterstaining was performed using 4ʹ,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) in TBS for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... 6 min) onto nitrocellulose membrane (0.2 μm pore diameter, iBlotTM Transfer stack, ThermoFisher, #IB23001/#IB23002) using the iBlot2 (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... QPCR analysis was performed with QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) using SYBR Green PCR Master Mix (Life Technologies ...