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Citations for Thermo Fisher :
5151 - 5200 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Real time PCR analysis was performed using a Step One Plus real-time PCR system (Applied Biosystems, Life Technologies, Thermo Fisher Scientific Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... To determine disruption of PFTK1 transcription the SuperScript III Platinum SYBR Green One-Step RT-PCR kit (Invitrogen, 204174) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... One microgram of the extracted RNA was used for reverse transcription (HIGH CAPACITY CDNA RT; Applied Biosystems ref: 4368814). Transcribed cDNA was diluted 5-fold and used for real-time quantitative PCR (QuantiTect SYBR Green ...
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bioRxiv - Cancer Biology 2022Quote: ... one of them was directly coupled with a fluorophore using the Zenon Labeling Kit (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions and then incubated for 20 min (for anti-CD68 antibody ...
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bioRxiv - Microbiology 2022Quote: ... COS-7 cells grown on 13 mm cover slips were transfected with either the empty pcDNA3.1(+) vector or one expressing the SARS-CoV-2 E protein using Turbofect transfection reagent (ThermoFisher). At 24 h pt ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed in triplicate with the EXPRESSS One-Step SuperScript qRT-PCR kit (ThermoFisher Scientific, catalog # 11781200). Gene-specific primers and probes are provided in Supplementary File 2 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with one of the following secondary antibodies: 1) donkey anti-mouse 488 (1:250; Invitrogen, USA); 2 ...
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bioRxiv - Neuroscience 2022Quote: ... 1:2000) and secondary antibody for one hour (Optogenetics experiment: Alexa Fluor 488 goat anti chicken, Invitrogen (cat# A11039), 1:400 ...
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bioRxiv - Microbiology 2022Quote: ... The RT-PCR was performed using the SuperScript III Platinum One-Step Quantitative RT-PCR system (Invitrogen, Carlsbad, CA) or Taqman Fast Virus 1-step master mix (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... reverse transcription and qRT–PCR were performed in one step using a SuperScript III Platinum Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... One ml of fd or Pf4 phage (5 mg/ml) was incubated with 100 µg A488 fluorescent dye (ThermoFisher) for 1 hour at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: Sample temperature was controlled by heat exchange with one of two circulating water baths (Accel 250 LC, Thermo Scientific, USA ...
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bioRxiv - Neuroscience 2022Quote: The pth2 in situ probe sequence was amplified using the Invitrogen SuperScript IV One-Step RT-PCR kit (ThermoFisher) using the following forward and reverse primers ...
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bioRxiv - Neuroscience 2022Quote: Real-time quantitative PCR was performed using Taqman™Assay Reagents using the Step One Detection System (Applied Biosystems), according to manufactures protocol ...
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bioRxiv - Physiology 2022Quote: ... Total RNA concentration per sample was measured using a NanoDrop Spectrophotometer (ND-ONE-W, ThermoFisher Scientific, Waltham, MA, USA). RNA (2000 ng ...
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bioRxiv - Molecular Biology 2022Quote: ... 1976) from the F1 individuals and qualification and quantification of DNA were performed with NanoDrop One spectrophotometer (Thermo Scientific). On the F2 and F3 samples a rapid Chelex100-based procedure (Walsh et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR assays were performed with SuperScript III Platinum SYBR Green One-Step qTR-PCR Kit (Invitrogen Cat#11736051) according to vendor’s standard procedure ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM EDTA) in the presence of one tablet of the protease inhibitor cocktail per 25 ml (Complete, Invitrogen). Cell lysates were analyzed by 12% SDS-PAGE for 80 min at 100 V (Mini-Protean Cell ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos at the desired developmental stage were fixed for one hour using MAD fixative (2 parts methanol [Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Real-time PCR was performed with a Step One Plus Detection System (Real-Time PCR System-Thermo Fisher Scientific). The results represent the average from three technical replicates for each analyzed animal ...
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bioRxiv - Microbiology 2024Quote: The concentration of the DNase treated RNA was determined by measuring the A260/A280 ratio (Nanodrop One, Thermo Scientific). A 25 μl reaction was prepared for each sample using the following TaqMan reverse transcription reagents ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein concentrations were determined by measuring UV absorbance at 280 nm with calculated molecular weights and extinction coefficients based on ProtParam EXPASY tool (https://web.expasy.org/protparam/) using a NanoDrop One spectrophotometer (Thermo Fisher). Aliquots of purified protein in 1x PBS ...
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bioRxiv - Microbiology 2024Quote: Real-time RT-PCR was performed using AgPath-ID™ One-Step RT-PCR reagents (Applied Biosystems, Product # 4387424) on a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were cultured in the presence of 2.5 µM BrdU for one population doubling before 0.2 µg/ml colcemid (Gibco 15212012) treatment for the final 4 hours before trypsinization ...
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bioRxiv - Microbiology 2023Quote: ... One of six qPCR protocols were used (Cepheid GeneXpert, Cobas8800, Cobas Liat, DiaSorin MDX, Saliva COVID, or ThermoFisher Amplitude). One of five variant categories were assigned from whole genome sequencing (Alpha ...
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bioRxiv - Genetics 2024Quote: Neutral lipids in one-day-old synchronized adults were visualized by Oil Red O dye (Thermo Fisher Scientific, USA) staining using a published protocol [77] ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was stained with fluorophore-conjugated secondary antibodies at room temperature for one hour (Thermo Fisher Scientific A11001 + A11035), before post-staining with DAPI and phalloidin-633 (Thermo Fisher Scientific A22284 ...
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bioRxiv - Immunology 2024Quote: ... One cm pieces of small intestine were then incubated in HBSS containing 3 mM EDTA and 7.5% FBS (ThermoFisher) for 1 hour in a shaker at 37° C ...
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bioRxiv - Microbiology 2024Quote: ... These plasmids were transformed into chemically competent Escherichia coli cells (One Shot™ TOP10, ThermoFisher Scientific, New Jersey, USA) and grown in liquid Lysogeny Broth (LB ...
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bioRxiv - Biochemistry 2024Quote: ... the medium was replaced one containing 900 µg/ml geneticin (G418, Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA). The medium was changed every three days ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) reaction was carried out with Step-One Plus system (Thermo Fisher Scientfic, USA).
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bioRxiv - Developmental Biology 2023Quote: ... qPCR with diluted cDNA was performed in duplicate for all tested genes with the Step One system (Applied Biosystems) and Fast SYBR™ Green Master Mix (4385612 ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated for one hour at room temperature with 100 μl CCF2/AM dye (Thermo Fisher Scientific #K1032). Afterwards ...
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bioRxiv - Pathology 2023Quote: ... PCR and data acquisition was performed using the AB7300 Real-Time PCR thermal cycler with Step One software (v2.2.2; Applied Biosystems). eef1al1 - eukaryotic elongation factor 1 alpha like 1 and rpl13a - ribosomal protein L13a were used as housekeeping genes to normalize the mRNA expression levels ...
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bioRxiv - Genetics 2023Quote: ... One μg of the DNA was used as template for dsRNA synthesis using MEGAscript T7 Transcription kit (Thermo Fisher) at 37 °C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... one of which was subjected to DNase I treatment using the TURBO DNA-free kit (Thermo Fisher Scientific, Inc.). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... One µg of total RNA was reverse transcribed for each sample with RevertAid H Minus Reverse Transcriptase (Thermo Scientific). Quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Immunology 2023Quote: ... The final RNA concentration and quality were checked on a nanodrop (Nanodrop One, Thermo Fisher Scientific, Pittsburgh, PA, USA), and RNA was stored at -80□ until needed ...
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bioRxiv - Synthetic Biology 2023Quote: ... to 30 μL final volume and quantified using a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific). Gibson Assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... All qRT-PCRs were carried out on a Step One Plus Real-Time PCR System (Applied Biosystems, Life Technologies). Primers used are as follows ...
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bioRxiv - Cell Biology 2023Quote: ... All qRT-PCRs were carried out on a Step One Plus Real-Time PCR System (Applied Biosystems, Life Technologies). Primers used are as follows ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration was measured at 280 nm using a NanoDrop One Microvolume UV-Vis spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... The resulting products from the respective in-fusion cloning reactions were transformed into stbl3 One-Shot competent cells according to the manufacturer’s instructions (Invitrogen), to generate the Xlone plasmids of Xlone_SNAPf–TDP-43 ...
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bioRxiv - Genomics 2023Quote: ... Biotin pull down was initiated py pipetting 20 µL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific, Cat # 65306) into DNA lo-bind tubes ...
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bioRxiv - Genomics 2023Quote: ... One microliter of each cDNA sample was used for TaqMan PCR (Eppendorf RealPlex Mastercycler and Applied Biosystems QuantStudio 3) with 50 heating cycles at 94°C for 30 seconds ...
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bioRxiv - Physiology 2023Quote: ... and one of the following secondary antibodies: Donkey anti-rabbit Alexa Fluor 488 (Invitrogen; Catalog# A21206; 1:250 dilution), Donkey anti-goat Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... incubated for one hour at room temperature (1:1000, Alexa Fluor 488 Goat anti-Mouse IgG, Invitrogen A-11001), and similarly washed ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time qPCR for measuring mRNA levels was performed using Step-One-Plus real-time PCR System (Applied Biosystems) and the Fast SYBR Green Master mix (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... Biotin pull down was initiated py pipetting 20 μL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific, Cat # 65306) into DNA lo-bind tubes ...
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bioRxiv - Evolutionary Biology 2023Quote: Anaerobic conditions were produced by incubating the 96 deep well plates in an airtight 2.6L container with one Anaerogen 2.5L sachet (Thermo Scientific). The Anaerogen sachet rapidly absorbs oxygen and releases CO2 creating anaerobic conditions ...