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Citations for Thermo Fisher :
5051 - 5100 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... One μg of total RNA was used for complementary DNA synthesis using RevertAid First Strand cDNA Synthesis kit (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed three times with PBS and incubated for one hour with secondary antibody (Alexa Fluor 594, chicken anti-mouse 1:1’000, Invitrogen) in PBS with 0.1 % Triton X-100 and 6 % FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were exposed to 200μM H2O2 or a control for one hour before being transfected with the reporters using Lipofectamine 3000 (Invitrogen). Cells were lysed and compared through quantitative western blotting 24 hours post H2O2 treatment.
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bioRxiv - Neuroscience 2020Quote: ... reacted with one of the PCR primers and the BigDye terminator v3.1 cycle sequence kit (Thermo Fisher Scientific, 4337455), and the gene sequence was identified using a 3130 Genetic Analyzer (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... array of 48 different mouse genes using a Step One Plus RT PCR system (Applied Biosystems, Foster City, CA) modified based on a previously published array of oxidant/antioxidant genes ...
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bioRxiv - Biochemistry 2020Quote: ... The protein concentration was determined by measuring the A280 using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, MA, USA). Final protein was dispensed as 0.5 ml and 0.05 ml aliquots ...
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bioRxiv - Biochemistry 2020Quote: ... 0.01% Triton X-100) was dispensed to each well of one column of the plate using Multidrop (Thermo Fisher). For positive control ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 4T1 cells were injected and mice treated with either 1 or 3 doses of 8 mg/kg carboplatin or vehicle by tail vein injection staggered by one day with anti-VEGFR3 antibody (100 μg per injection × 3 total injections, I.P., eBioscience (now ThermoFisher), Control IgG ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of DNaseI-treated RNA was reverse-transcribed with oligo(dT) and the Superscript III enzyme (Thermo Scientific) in 20 μl reaction ...
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bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative expression was calculated using the ΔΔCT method ...
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bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative genomes were calculated using the ΔCT method ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were loaded into the chamber ports on one side of the Countess cell counting chamber slide (Invitrogen). Viable and dead cells were counted using the automated cell counter ...
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bioRxiv - Genomics 2021Quote: ... Samples were transferred to 1.5 mL safe-lock tubes containing one scoop of acid-washed glass beads (<106μM) and 1 mL TRIzol (Invitrogen) containing 20μg/mL GenElute-LPA (Sigma-Aldricht ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were purified by precipitation and one microgram each was labeled by nick translation (Invitrogen or Roche Diagnostics) with Cy3-dUTP (GE ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA concentration and purity were estimated in a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific) and the integrity was evaluated in a 2100 Bioanalyzer using an Agilent RNA 6000 Pico chip ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed on a Step One Plus Real-Time PCR system (Thermo Fisher, Waltham, MA, United States) with Power SYBR Green Master Mix protocol (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... and one or more of the following antibodies: goat anti-rabbit IgG Alexa Fluor 568 (1:1000, #A11036, Invitrogen), goat anti-mouse IgG Alexa Fluor 647 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... The real-time PCR was performed using Step one Plus real time PCR Assay (Applied Biosystems, Foster City, CA). All reactions were carried out using Power SYBR Green premix (Applied Biosystems).
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bioRxiv - Biochemistry 2022Quote: ... Cells remained in the dark with all fluorescent indicators followed by trypsinization and one wash with 1X PBS (Gibco). The indicators employed and fluorescence excitation/emission wavelengths were as follow ...
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bioRxiv - Neuroscience 2022Quote: ... One microgram of RNA was used to prepare cDNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative real-time PCR analysis was performed using a DyNAmo ColorFlash SYBR Green qPCR kit (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Intracellular RNA was quantified using the SuperScript III Platinum One-step SYBR Green master mix (Thermo Fisher, Basel, Switzerland) and these cycling parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... one of the wells was treated with 200 µg/mL G418 (Geneticin™ Selective Antibiotic, Thermo Fisher Scientific, 10131035). After 2 days ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.1%TBS-Tween for one hour and incubated with anti-Biotin (Invitrogen, Cat # 31852, RRID: AB_228243, 1:1000) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking buffer was replaced after one hour with 7 µg/mL mouse anti-occludin antibody (cat# 33-1500, Invitrogen) in PBS with 5% goat serum ...
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bioRxiv - Immunology 2021Quote: ... Real-time qRT-PCR was performed using SuperScript™ III Platinum™ One-Step qRT-PCR Kit (Invitrogen, USA) following the manufacturer’s instructions to detect NDV M gene64 and enable the calculation of NDV genome copies.
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bioRxiv - Microbiology 2020Quote: ... An agarose mixture comprised of one third Neutravidin Agarose resin and two thirds Pierce Control Agarose resin (ThermoFisher Scientific) was prepared to minimize contamination of samples with neutravidin from the beads ...
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bioRxiv - Microbiology 2021Quote: ... a 12.5 µl reaction with 2.5 µl RNA was done with the SuperScript III one-step reverse transcriptase-PCR system (Invitrogen) with the Platinum Taq DNA polymerase according to the manufacturers’ protocol and the primers indicated in Table S2 ...
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bioRxiv - Plant Biology 2021Quote: ... coli One Shot Top10 and subcloning efficiency DH5α chemically competent cells were performed according to the manufacturer’s instructions (Invitrogen). Reaction products were mixed with competent cells and incubated on ice for up to 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... one for virus quantitation (TCID50) and the other for vRNA extraction in TRIzol (ThermoFisher, 15596026; 1 in 3 dilution). Samples were stored at -80°C until required ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated at 37°C for one hour and then overlaid with 1.2% Avicel (FMC biopolymers) in Opti-MEM I (1X) + GlutaMAX (Gibco) for 72 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for one series included rabbit anti-beta Amyloid (1:300, Cat# 715800, Lot# SH257822; Invitrogen, Waltham, MA). Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Quality and quantity of the isolated RNA was measured with a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Scientific). Total RNA of 4T1 cells with either knockdown of PHGDH or control (3 replicates each ...
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bioRxiv - Cancer Biology 2021Quote: ... Quality and quantity of the isolated RNA was measured with a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Scientific). RNA was reverse transcribed into cDNA using a qScript cDNA Synthesis Kit (Quantabio) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentration and purity of the isolated RNA was measured with a UV spectrophotometer (NanoDrop™ One, Thermo Scientific). Total RNA was used for cDNA synthesis in a reaction containing 3μM random primer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Behavioral tests were carried out in one well Lab-Tek II coverslip chambers (Thermo Fisher Scientific, Waltham, MA, USA). We cut out strips of each of the three buffered gels with lengths equal to the chamber length ...
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bioRxiv - Immunology 2022Quote: ... Reactions were conducted on the Step One Real-Time PCR System using the SYBR-green fluorescence system (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... After one washing step (1% BSA/PBS) cells were permeabilized in PermBuffer (saponin-based permeabilization and wash buffer; ThermoFisher) for 15min and subsequently stained with pAKT Ser473 (CellSignaling ...
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bioRxiv - Evolutionary Biology 2022Quote: ... One sgRNA targeting the first exon NeNaC2 were synthetized in vitro using the MEGAshortscript T7 kit (Thermo Fisher Scientific) using the following oligos for NeNaC2 ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of RNA was reverse-transcribed to cDNA using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific). RT-qPCR reactions were prepared with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection of cells was performed one day after passaging with a cell confluency of 70% and Lipofectamine 2000 (Invitrogen) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: 293T cells were transfected with eight pHW2000 plasmids each encoding one of the IAV segments using Lipofectamine 2000 (Invitrogen). Cells were incubated at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... the membrane was stripped for one hour at room temperature using Restore™ Western Blot Stripping Buffer (ThermoFisher Sci.) to remove the anti-FLAG antibody and was blocked at room temperature for one hour in Blotto followed by incubation with anti-β-tubulin monoclonal antibody (E7 concentrated from Developmental Studies Hybridoma Bank ...
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bioRxiv - Developmental Biology 2020Quote: ... The walls of the chamber slides were removed and one drop of Prolong Diamond mounting media (Thermo Fisher Scientific) was added to each well ...
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bioRxiv - Molecular Biology 2019Quote: ... were maintained in monolayer culture at 37°C and 5% CO2 in one of the following medium: Medium A contained DMEM (4.5 g/L glucose; Gibco) supplemented 100 U/ml penicillin and 100 µg/ml streptomycin sulfate ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Samples were sequenced in one direction for each gene using an automated 3130xl DNA Analyzer (Applied Biosystems, CA, USA) and the sequences were aligned using CLC Main Workbench 5.0.2 (CLC Bio ...
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bioRxiv - Microbiology 2019Quote: ... and an endogenous control transcript (Ga0102493_112759) were evaluated using TaqMan probes and SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) with a QuantStudio 5 real time PCR system (Thermo Fisher) ...
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bioRxiv - Physiology 2019Quote: ... all the islets from one mouse each were dissociated into single cell suspension using a nonenzymatic buffer reagent (Gibco) and counted with trypan blue ...
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bioRxiv - Microbiology 2019Quote: ... Total DNA concentration was measured using a NanoDrop™ One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA) and DNA integrity was determined by 260/280 ratio ...
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bioRxiv - Immunology 2019Quote: ... the qRT-PCR was performed using SuperScript III Platinum One-Step Quantitative RT-PCR System (Invitrogen, Carlsbad, CA, USA) on a LightCycler 480 or LightCyc instrument (Roche Diagnostics ...
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bioRxiv - Genomics 2020Quote: ... and the DNA quality and quantity was tested using both NanoDrop One UV-Vis spectrophotometer (Thermo Fisher Scientific, USA) and Qubit 3.0 Fluorometer (Invitrogen ...