Labshake search
Citations for Thermo Fisher :
5251 - 5300 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... Wells were washed 3 times and incubated with 1 μg/ml biotinylated anti-human IgE (A18803, Invitrogen) in 1% skim milk for 2 hours at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were washed 3 times with PBS and an aliquot of 1 ml of warmed TrypLE (Gibco) added to each well and incubated at 37°C for 10 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 mM EDTA) for 3 hours at room temperature using Slide-a-Lyzer mini dialysis cups (ThermoFisher), diluted to 100 μM ...
-
bioRxiv - Immunology 2021Quote: ... with confluent bEnd.3 cell monolayers or pre-coated with collagen I (1 mg/ml, Life Technologies), and allowed to adhere for 10 min ...
-
bioRxiv - Neuroscience 2022Quote: ... Slides were washed 3 × 10 min in TBS-T and secondary antibody incubation (1:750; Life Technologies) was performed for 1 hr ...
-
bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
-
bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... 3 % of homogenate was added to 1 ml of TRIzol Reagent (Fisher Scientific cat# 15-596-026) and RNA was isolated as per the user guide referenced above ...
-
bioRxiv - Neuroscience 2022Quote: ... biocytine filled neurons were revealed using Streptavidin Alexa Fluor 488 (1:1000; 3 h in dark; Invitrogen). Slices were then washed in PBS (1 h ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1μg total plasmid DNA (1:3 SAM to gRNA ratio) was diluted in Opti-MEM (Gibco #31985062) and mixed with p3000 reagent ...
-
bioRxiv - Cell Biology 2022Quote: ... pDEST-AD-CYH2 (assay version 1) and pDEST-AD-AR68 (assay version 3) using Gateway Technology (Invitrogen). Attributes of these plasmids are summarized in Suppl ...
-
bioRxiv - Molecular Biology 2024Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (ThermoFisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2024Quote: ... 150ng of DNA was run on 3 lanes of a 1% eGel (Thermo Fisher Scientific, Waltham, MA) and size-selected into two populations ...
-
bioRxiv - Bioengineering 2024Quote: ... for 1 hour before blocking with 3% (w/v) bovine serum albumin (BSA, Fisher Scientific, Cat # BP9703100). To detect ARP3 and GLUT4 ...
-
bioRxiv - Molecular Biology 2024Quote: ... complexes (DNA to PEI MAX ratio, 1:3, w/w) formed in Opti-MEM (Thermo Fisher Scientific) via incubation for 15 min at 25°C ...
-
bioRxiv - Genomics 2024Quote: ... cells were transfected with 1 µg of plasmid library using 3 µl Lipofectamine 3000 reagent (L3000008, Invitrogen, Carlsbad ...
-
bioRxiv - Neuroscience 2023Quote: ... The slices were incubated in Cyanine 3 (Cy3) goat anti-mouse (Thermo Fisher Scientific, A10521, 1:500) and Alexa Fluor 488 goat anti-rabbit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were individually mixed with an equal volume of 1 µg/ml of 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (DiI; Invitrogen), incubated for 15 min on ice ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were then washed with PBS 3 times and incubated with 1:10,000 DAPI (Invitrogen, Cat#D1306) for 5 minutes ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 μg DNA and 3 μl transfection reagent were mixed in 100 μL Opti-MEM media (GIBCO) for 20min at room temperature before the mixture was added into each well ...
-
bioRxiv - Genomics 2022Quote: ... The concentrate (about 300-450 µl) was mixed 1:3 with Trizol LS (ThermoFisher cat# 10296-010), and the RNA extracted using the DirectZol RNA kit (Zymo cat# R2052) ...
-
bioRxiv - Immunology 2023Quote: ... Lysate was diluted 1:3 in distilled water and digested in 0.2mg/ml proteinase-K (Invitrogen; 25530049) for 10 minutes at 55°C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) + Tris Buffer HCl pH 7,4 (1:1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 1:3 (µg DNA: µg PEI) in 200 µl Opti-MEM (Gibco, 31985070). Media was replaced after 24h ...
-
bioRxiv - Cell Biology 2024Quote: Cells were washed 2x with PBS and gently dissociated with 1:3 diluted Accutase (Gibco, A11105-01). Lifted cells were washed 2x with cold dPBS (HyClone ...
-
bioRxiv - Neuroscience 2024Quote: ... the slices were incubated in Cyanine 3 (Cy3) goat anti-mouse (Thermo Fisher Scientific, A10521, 1:500) in PBS-Triton X 0.3% ...
-
bioRxiv - Bioengineering 2024Quote: ... glass coverslips were submerged in piranha solution composed of 3:1 sulfuric acid (H2SO4, Fisher Scientific, A300) and 30 wt% hydrogen peroxide (H2O2 ...
-
bioRxiv - Microbiology 2019Quote: ... 5 to 6 seeds were pipetted into 120 x 120 mm square petri dishes (Thermo Fisher Scientific) containing half-strength Murashige and Skoog salts (MS) ...
-
bioRxiv - Cancer Biology 2022Quote: ... real-time quantitative PCR was done using the ABI Quant Studio 5 and 6 (Life technologies, USA). The cDNA was used as template and DyNAmo Flash SYBR Green qPCR kit (#F-416L ...
-
bioRxiv - Cell Biology 2022Quote: ... about ∼5 μg of bacmid were transfected using 6 μl of Cellfectin II reagent (Thermo Fisher Scientific). 5 days after initial transfection ...
-
bioRxiv - Immunology 2021Quote: ... Sorted memory CD4+ T cells were labelled with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE, ThermoFisher) and cultured at a ratio of 2:1 with irradiated autologous monocytes untreated or pulsed for 3 h with recombinant SARS-CoV-2 Spike protein (2.5 μg/ml) ...
-
bioRxiv - Cell Biology 2022Quote: ... Transfection with 5 ug total DNA in a 6 well plate was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
-
bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
-
bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
-
bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
-
bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
-
bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
-
bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
-
bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Embryos were then incubated in the NGS for at least 4 hrs and incubated overnight at 4°C with secondary antibodies: goat anti-rabbit Alexa Fluor 488 (Invitrogen Cat# A11070; 1/400) and goat anti-mouse HRP-conjugated (Thermo Fisher Scientific Cat# G-21040 ...
-
bioRxiv - Bioengineering 2023Quote: ... in 2.5 μl NuPAGE® LDS Sample Buffer (4×) and 1 μl NuPAGE® Sample Reducing Agent (10×)] were loaded in Bolt 4-12% Bis-Tris Plus Gels (Thermo Fisher Scientific, USA). Samples were denatured at 95°C for 3 mins ...
-
bioRxiv - Molecular Biology 2023Quote: ... and brain tissues were weighed and homogenized at a density of 140 mg of dry tissue per milliliter in ethyl acetate / isopropanol (4:1) using a Bead Mill 4 homogenizer (Thermo Fisher Scientific, Waltham MA) and 2-mL pre-filled polypropylene microtubes (2.8 mm ceramic beads ...
-
bioRxiv - Microbiology 2023Quote: ... A NanoDrop One (ThermoFisher) was used to measure the RNA concentration and purity ...
-
bioRxiv - Genomics 2023Quote: ... Nanodrop One (Fisher Scientific) for purity ...
-
bioRxiv - Pathology 2023Quote: ... were incubated with 10 µM red fluorescent Lipophilic Tracer DiD (1,1’-dioctadecyl-3, 3, 39, 39-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt; Thermo Fisher Scientific, Waltham, MA, USA) and/or 2 mM SYTO RNA-Select Green Fluorescent Cell Stain Kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... A recombinant lentivirus vector expressing the coding sequence of the EBV transactivator BZLF1 under control of a tetracycline-regulated promoter was constructed by cloning the open reading frame amplified with the primers 5’-CGACCGGTATGATGGACCCAAACTCGAC-3’ and 5’-CGACGCGTTTAGAAATTTAA GAGATCCTCGTGT-3’ into the Age I and Mlu I sites of the pTRIPZ lentiviral vector (Thermo Fisher Scientific, USA). For virus production ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged at 14,000g for 5 minutes at 4°C and electrophoresed on NuPAGE™ 4-12% Bis-Tris Polyacrylamide gels (Thermo Fisher) with NuPAGE™ MOPS running buffer (Thermo Fisher ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...