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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: One-step RT-PCR was realized with the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Thermo Fisher Scientific) on an iCycler Thermal Cycler (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR products were amplified from extracted RNA using a SuperScript™ III One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA). A 20 μl reaction was assembled in PCR 8-tube strips through the addition of 10 μl 2× reaction mix ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using the Superscript III one-step RT-PCR system with platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA) under the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... was generated for each subject from 2,000 HIV-1 RNA copies by RT-PCR using SuperScript™ One-Step RT-PCR (Invitrogen, Carlsbad, California) followed by amplification using GoTaq colorless Master Mix (Promega ...
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bioRxiv - Microbiology 2020Quote: ... real-time RT-PCR was performed using the SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase (Invitrogen) on the CFX96 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR was performed using QuantiTect Probe RT-PCR Kit (Quiagen®) in an ABI PRISM 7500 Sequence Detection System (Applied Biosystems). Amplifications were carried out in 25 µL reaction mixtures containing 2× reaction mix buffer ...
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bioRxiv - Cell Biology 2019Quote: ... according to the manufacturer’s instructions and was used as the template for RT-PCR using Superscript IV One-Step RT-PCR System (Invitrogen, 12594-025). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... An 800 bp fragment covering the ORF10 region was amplified by one-step RT-PCR using the SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThermoFisher) and the following primers (OL11F ...
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bioRxiv - Neuroscience 2023Quote: Quantification of transcripts was performed by reverse transcription quantitative PCR (RT-qPCR) using the SYBR® Green RT-PCR assay (Applied Biosystems). Amplification ...
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bioRxiv - Microbiology 2023Quote: ... the extracted vRNA was converted to double-stranded cDNA and amplified using a one-step RT-PCR using SuperScript III One-Step RT-PCR kit (Thermo Fisher Scientific). The primers used were as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using (Lipofectamine RNAiMAX, Life Technologies). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2020Quote: ... The expression of miR-155 and cel-miR-39 were analyzed by specific Taqman assays (ThermoFisher). Cel-miR-39 was used as an endogenous control to normalize expression ...
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bioRxiv - Cell Biology 2022Quote: ... site-directed mutagenesis was performed as described in Liu & Naismith (32) using primers 5’-ACTACTTCGATGAGATCGCTCTGCTCATGAACCGTCCTCGTGCTG and 5’-AGCGATCTCATCGAAGTAGTCAGACGGTGCGAGTCTTCCAACCTC using Phusion Plus DNA polymerase (#F630S, ThermoFisher). Following confirmation of the RIαB G323D mutation by Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and an ABI 7900HT RT-PCR machine (Applied Biosystems). Expression levels were normalized to the expression level of the housekeeping gene HMBS and quantitative changes were calculated using the method described in (48) ...
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bioRxiv - Cell Biology 2022Quote: ... TaqMan RT-PCR technology (7500Fast, Applied Biosystems, CA, USA) was used to determine the expression levels of selected target genes with TaqMan sitespecific primers and probes (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... on a StepOne Plus RT-PCR System (Applied Biosystems).
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bioRxiv - Physiology 2021Quote: ... TaqMan real-time RT-PCR assay (Thermo Fisher Scientific): mRNA expression of SMA (Hs00426835_g1) ...
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bioRxiv - Physiology 2020Quote: ... using the SuperScript III RT-PCR kit (Life Technologies), employing reverse transcriptase in the presence of deoxynucleotides (dNTP ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR Step One Plus system (#8024, Applied Biosystems) was used.
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bioRxiv - Cell Biology 2021Quote: ... and QuantStudio 3 RT-PCR system (Thermo Fisher Scientific). All expression levels were normalized to that of RPS18 mRNA ...
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bioRxiv - Genetics 2022Quote: ... RT-PCR was performed using PowerUP Sybr (ThermoFisher, #A25741) based on manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative RT-PCR was performed using StepOnePlus (Applied Biosystems). Relative expression was calculated using the ΔΔCt method with the reference gene ef1α ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were run on AgPath RT-PCR (Life Technologies) kit on a QuantStudio™ 7 Flex Real-Time PCR System and the relative amounts of FAM and HEX signal used to determine the proportion of Omicron and Delta in the original samples ...
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bioRxiv - Bioengineering 2020Quote: ... with an ABI 7500 RT-PCR System (Applied Biosystems). Data were analyzed with 7500 Software using relative quantification analysis.
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bioRxiv - Genetics 2020Quote: ... RT-PCR was performed using SuperScriptIII Reverse Transcriptase (ThermoFisher) with random primers ...
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bioRxiv - Cancer Biology 2021Quote: ... and RT-PCR Grade Water (Invitrogen, Carlsbad, CA, USA). Using a C100 thermal cycler (BioRad) ...
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bioRxiv - Developmental Biology 2022Quote: ... in a StepOne Plus RT-PCR apparatus (Applied Biosystems). Gene expression was assessed by the comparative CT (ΔΔCt ...
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bioRxiv - Molecular Biology 2022Quote: ... Using Verso 1-Step RT-PCR kit (Thermo Scientific), reactions of 13 μl final volume was set up using 0.6 μM of triplex primer cocktail in equimolar ratio of labelled forward and reverse primers of each target ...
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bioRxiv - Biochemistry 2023Quote: ... A QuantStudio 3 RT-PCR machine (Thermo Fisher Scientific) was used to measure melting temperatures.
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bioRxiv - Plant Biology 2023Quote: ... on a QuantStudio 5 RT-PCR system (Applied Biosystems). Sequencing was performed at the University of California Davis Genome Center using a HiSeq 4000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... and conducted using the StepOnePlus RT-PCR machine (ThermoFisher). All data was normalized to endogenous controls GAPDH (human ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-PCR was conducted using validated TaqMan assays (ThermoFisher, Hs00271949_m1 for TBX1 ...
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bioRxiv - Developmental Biology 2023Quote: ... on a 7300 fast RT-PCR System (Applied Biosystems) with pre-designed exon-spanning reaction mixes (ThermoFisher Scientific) ...
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bioRxiv - Pathology 2023Quote: ... cDNA was amplified with RT-PCR master mix (ThermoFisher) and ran in Bio-Rad PCR Thermal Cycler ...
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bioRxiv - Cancer Biology 2023Quote: ... and Quantstudio 7 flex RT-PCR system (ThermoFisher Scientific). Expression was measured and the ΔCT was calculated ...
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bioRxiv - Neuroscience 2023Quote: ... in a QuantStudio3 RT-PCR system (Applied Biosystems™) based on manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2022Quote: ... Total expression levels of miR-675-3p and miR-675-5p were determined relative to snoRNA202 by using a separate RT kit (TaqMan MicroRNA Reverse Transcription Kit, Thermo Fisher Scientific), qRT-PCR primers (Assay Id 001232 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the reverse-transcriptase reaction using RT primers and SuperScriptIII (Thermo Fisher Scientific). cDNA libraries were amplified by means of Phusion High-Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... before reverse transcription with Superscript III RT using random primers (200 ng/reaction, Invitrogen). Quantitative PCR reactions were carried out with the indicated primer pairs (Eurofins MWG Operon ...
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bioRxiv - Molecular Biology 2020Quote: ... before reverse transcription with Superscript III RT using random primers (200 ng/reaction, Invitrogen). Quantitative PCR reactions were carried out with the indicated exonic primer pairs (Eurofins MWG Operon ...
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bioRxiv - Physiology 2020Quote: ... and custom Multiplex RT Primer pool in a SimpliAmp™ Thermal Cycler (Applied Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... reverse transcription was performed using oNTI223 primer and superscript III RT kit (Life Technologies). The cDNA products were separated on a 10% polyacrylamide TBE-urea gel and only those fragments migrating between 100-500 bp were excised and recovered by gel extraction ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs were synthesized using random primers and Moloney murine leukemia virus RT (Invitrogen). The RT-PCR products were separated on a 2% w/v agarose-TBE gel ...
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bioRxiv - Cell Biology 2022Quote: ... TaqMan RT primers against human occludin (Hs05465837_g1) and GAPDH (Hs02786624_g1) were purchased from Invitrogen.
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bioRxiv - Microbiology 2021Quote: ... These primers were used for cDNA synthesis (Thermo Scientific RevertAid RT Reverse Transcription Kit) according to manufacturer’s instruction with an annealing step at 16°C instead of 25°C and were used in multiplex to target respective fungal sRNAs and barley miRNAs Hvu-mir159 and Hvu-mir168 as references ...
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bioRxiv - Microbiology 2022Quote: ... RNA was converted into cDNA using random hexamer primers and Revert aid RT (Invitrogen). Selected candidates were then analyzed by RT-qPCR using specific primers ...
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bioRxiv - Bioengineering 2022Quote: ... or a negative control miR (Invitrogen mirVana miRNA Mimic ...
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bioRxiv - Molecular Biology 2020Quote: ... and miR-183-5p (Applied Biosystems). Reverse transcription products were then amplified by quantitative PCR (95 °C ...
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bioRxiv - Microbiology 2019Quote: ... total RNA was reverse transcribed using virus-specific RT primers or oligo-dT primers and the SuperScript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA). Real-time quantitative PCR was performed on the Applied Biosystems 7500 system with QuantiTect SYBR Green PCR Master Mix (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reaction set-up included 2.5ul of 10X PCR Buffer (Thermo Scientific, USA), 1.5 mM MgCl2 ...