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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Human embryonic kidney cells (HEK-293, ATCC) were grown in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (Gibco). Cell lines were regularly screened for mycoplasma contamination by DAPI staining ...
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bioRxiv - Biophysics 2022Quote: ... HEK 293 cells and COS-7 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, USA) supplemented with glucose (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... in a pcDNA5/FRT/TO/n2SC or pcDNA5/FRT/TO/c2SC vector with 0.9 µg of pOG44 plasmid into HEK 293 Flp-In T-REx cells (Invitrogen). Cells were selected for 2 weeks in DMEM containing 100 µg/mL hygromycin and 15 µg/mL blasticidin ...
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bioRxiv - Developmental Biology 2023Quote: HEK-293 (ATCC CRL-1573) was maintained in DMEM supplemented with 10% fetal bovine serum and penicillin/streptomycin (Invitrogen). Cells were cultured according to the manufacturer’s instructions (ATCC) ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293 cells were purchased from ATCC and maintained in 10% (v/v) FBS (Gibco, Cat. no. 10270-106) and 100 U ml-1 penicillin and 100 µg ml-1 streptomycin (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were purchased from ATCC and cultured in 10% (v/v) FBS (Gibco, Cat. No. 10270-106) and 100U mL-1 penicillin and 100µg mL-1 streptomycin (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were purchased from ATCC and maintained in 10% (v/v) FBS (Gibco, Cat. no. 10270-106) and 100U ml-1 penicillin and 100 µg ml-1 streptomycin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: HEK 293 cells (ATCC, Manassas, VA) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, high glucose; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: ... HEK 293F cells were grown in suspension in 100 mL of unsupplemented Freestyle 293 media (Life Technologies, 12338-018) until cell density reached 2 × 106 cells/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T (Human Embryonic Kidney cells 293) cells were obtained from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... the expression vector was used to transfect human 293-EBNA cells (Invitrogen) and serum- free medium was collected for protein purification according to established methods [51] ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney (HEK293; Invitrogen’s Flp-In-293 cell line cat #R75007) cells were grown and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Biochemistry 2022Quote: HEK293 (Human Embryonic Kidney 293-Flp-In T-Rex, Thermo Fisher Scientific) cells were cultured in DMEM (Dulbecco’s modified Eagle‘s medium ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293T cells were cultured in DMEM (HyClone) supplemented with 10% heat-inactivated FBS (HI-FBS; ThermoFisher). CHLA-255-Luc-EGFP cells were cultured in IMDM (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... Human embryonic kidney 293T cells (HEK-293T) were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2021Quote: Suspension cultures of human embryonic kidney (HEK) 293F cells (purchased from Invitrogen) were maintained using 293 freestyle media (gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Pooled neonatal primary Human Epidermal Keratinocytes (HEK) were obtained from Life Technologies and were used between passages two to five ...
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Cancer Biology 2021Quote: ... was then gently mixed with 20 parts (v/v) of HEK-293 cells (ATCC) suspended at a density of 2 x 105 cells/mL in DMEM (Gibco) + 10% FBS (Seradigm/VWR) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... HEK-293 cells were transferred with a density of 12000 cells per well in white 96-well plates (Thermo Scientific™ Nunc™ F96 MicroWell™ ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T and T-Rex 293 cells were grown in Dulbecco’s modified Eagles medium (DMEM, Thermo Fisher scientific, MA, USA) containing 4.5 mg/ml glucose ...
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bioRxiv - Neuroscience 2020Quote: ... Lentiviruses were produced by transfecting HEK 293 cells with lentiviral vectors together with packaging vectors (3rd generation) using Lipofectamine 3000 (Thermofisher). Viral supernatant was collected 24hr and 48hr post-transfection ...
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bioRxiv - Genetics 2022Quote: HEK 293 cells were cultured in medium consisting of high glucose DMEM (Hyclone) supplemented with 10% fetal bovine serum (Gibco) and 1× penicillin-streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... The pSAd36-S and pSAd-control plasmids were linearized with PacI restriction enzyme to liberate viral genomes for transfection into T-Rex 293-HEK cells (Invitrogen). The rescued replication-incompetent ChAd-SARS-CoV-2-S and ChAd-Control vectors were scaled up in 293 cells and purified by CsCl density-gradient ultracentrifugation ...
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bioRxiv - Microbiology 2022Quote: The viability of HEK-293 cells post exposure to films was also determined using a LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen). Approximately 105 cells were seeded per well ...
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bioRxiv - Molecular Biology 2022Quote: pcDNA5/FRT/TO-FLAG-Angel1 WT was used to generate stable HEK FLp-In T-REx-293 cell lines (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: The original CC1 construct with super ecliptic pHluorin (SE) A227D (31) or CC1 variants with mutations in S1 were expressed in HEK 293 cells via lipofectamine (Invitrogen). Coverslips with transiently transfected cells were placed into a bath chamber (Warner instruments ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% Foetal Bovine Serum (FBS) (HEK 293) or Calf Serum (NIH 3T3 and Ptch1−/− MEFs) containing 1% Glutamax (Gibco). All cell lines were grown at 37°C in 5% CO2 and passaged prior to reaching confluence.
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293 (CRL-1573) cells were obtained from ATCC and maintained in high-glucose (4.5 g/L) DMEM (Gibco 11995073) supplemented with 4 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... lentiviruses (for target sequences see S1 File) for shRNA delivery were produced by transfecting HEK-293 cells in a 60mm dish with a 1ml OptiMEM (Gibco) mix containing 15µl of Lipofectamine-2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63] ...
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bioRxiv - Neuroscience 2023Quote: ... we plated HEK-293 cells (ATCC catalog # CRL-1573) with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63] ...
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bioRxiv - Cancer Biology 2024Quote: ... cell lines were purchased from ATCC and maintained in DMEM (HEK-293 and HeLa) or RPMI 1640 (SNU-16) supplemented with 5% fetal bovine serum (FBS, Gibco) and penicillin/streptomycin in a humidified 37°C incubator with a 5% CO2 supply.
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bioRxiv - Molecular Biology 2024Quote: The Su9-AsCas12a gene was introduced into HEK-293 T-REx cell line nuclear genome using the Flp-In System (Invitrogen). T-REx-293 cell line was transfected in a 6-well plate with a 1:1 ratio of pOG44 Flp-Recombinase expression vector (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... and HEK-293 cells were seeded at 24,000 cells per well in 96-well plates (Thermo Scientific Nunclon Delta Surface). 5-Fluorouracil and compounds 1 to 10 were added in dimethylsulfoxide (DMSO ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Immunology 2024Quote: ... HI samples were incubated with 1 mL of IgG Fc Capture Select (Thermo Fisher) resin at 4C for 20 to 48 h to bind IgG ...
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bioRxiv - Biophysics 2020Quote: ... and inserted into a human Fc expression vector (Invitrogen). The proteins were expressed in HEK293T cells ...
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bioRxiv - Microbiology 2024Quote: ... or Goat anti-Human IgG Fc recombinant (ThermoFisher Scientific) antibodies were pre-coupled to Alexa Fluor 647 and used as secondary antibody in flow cytometry experiments ...
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bioRxiv - Molecular Biology 2024Quote: Human KPNA2 cDNA was cloned into His vector pRSET (Invitrogen) using BamHI and XhoI restriction sites27 ...
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bioRxiv - Biochemistry 2022Quote: ... Human FLP-In™ T-REx™ 293 Cell Lines (Invitrogen™, R78007) expressing hnRNPA1 wild type or D262V mutant cDNAs were generated by cotransfecting the hnRNPA1 cDNAs with a FLP recombinase expression plasmid according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and transfected into human Flp-In T-REx 293 cells (Invitrogen, Carlsbad, CA). Following hygromycin selection and cell expansion ...
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bioRxiv - Cell Biology 2020Quote: ... Identified and characterized plasmids containing the ACE2 isoform open reading frames were transfected into the HEK-293 cells using TurboFect reagent (ThermoFisher Corp.). 24 hours post-transfection the cells were harvested and processed for analysis by western blot according to established procedures [21] ...
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bioRxiv - Cell Biology 2022Quote: ... Ciliogenesis was induced by culturing RPE1 cells in medium without serum during 48 hours. HEK 293 cells (kind gift from F. Causeret, Institut Imagine, Paris) were cultured in DMEM medium (ThermoFisher Scientific) supplemented with 10 % fetal calf serum and antibiotics as previously ...
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bioRxiv - Cell Biology 2021Quote: ... the HEK 293 BRI3BP-HA cell line was transfected with control or BRI3BP silencer select siRNAs with Lipofectamine RNAi Max (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... DMEM (for A-431, HEK-293, NTERA-2, SH-SY5Y) or Ham’s F-12K (for A549) media (by Gibco or Euroclone) containing 10% fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: 100µg of the pCI PR8 NP:WT-C-His or pCI PR8 NP:F346S-C-His plasmids were transiently transfected into 100mL cultures of HEK Expi-293-F cells with ExpiFectamine according to the company protocol (Thermo Fisher). The transfected cells were pelleted (1000rpm ...
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bioRxiv - Genomics 2021Quote: ... Reporter plasmids (2 μg) were transfected into HEK-293 cells in 6-well plates at ∼60-90% confluency using Lipofectamine 3000 (ThermoFisher Scientific). Cells were harvested after 1 day by aspirating the media and resuspending the cells in 1 mL Trizol reagent (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...