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Citations for Thermo Fisher :
451 - 500 of 631 citations for R + SCH 23390 hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... ECM (3447-020-01, Cultrex 3D Collagen I, R&D Systems in 1M Hepes, Gibco and 37 mg/mL NaHCO3) was prepared ...
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bioRxiv - Cell Biology 2024Quote: ... h-prASC or r-prASC were seeded at 75.000 or 250.000 cells/well in non-adherent 6-well plates (Thermo Fisher Scientific) containing STD-M (+) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNP complexes were formed by combining 3 μM of each sgRNA and 2 μM SpCas9 NLS recombinant nuclease in R buffer (NeonTM NxT Eletroctroporation System 10-μL Kit, cat. no. N1025, ThermoFisher) for 10-20 min at RT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each piece of the intestine or whole head kidney was placed into a sterile 24 well plate on ice with 2 mL on R-90 media (90% (v/v) RPMI 1640 (+L-glutamine, -Phenol red; Gibco) + 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked with 5% BSA in TBST buffer and probed with anti-mouse Tim-3 goat polyclonal antibody (R&D) and detected with anti-goat-HRP antibody and SuperSignal West Pico Chemiluminescent reagent (Thermo Fisher).
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bioRxiv - Evolutionary Biology 2021Quote: ... A 3’ Venus tag was added by recombining the Nvsix3/6 coding sequence into pSPE3-R-Venus destination vector using the Gateway LR cloning reaction (ThermoFisher Scientific). mRNA synthesized and injected at 300ng/µL using previously described methods 61 ...
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bioRxiv - Microbiology 2022Quote: ... The region of recombination (VP1 to 2C) was amplified using primers PV3-F (5′-GCAAACATCTTCCAACCCGTCC-3′) and PV1-R (5′-TTGCTCTTGAACTGTATGTAGTTG-3′) and Taq polymerase (Life Technologies) with an initial denaturing at 94°C for 3 min ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... using the oligonucleotides WPRE-F 5’-TGCTTCCCGTATGGCTTTCAT-3’ and WPRE-R 5’-CAGCAAACACAGTGCACACC-3’ as primers and SYBR Select Master Mix (ThermoFisher Scientific). The measurements were performed with a CFX384 instrument (Biorad ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was treated with or without (Mock) RNase R (2 U/μg) in the presence of Ribolock(2 U/μg) (ThermoFisher Scientific). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1ug of the untreated RNA and from the same amount of RNAse R treated RNA using random hexamer and Superscript II reverse transcriptase (Invitrogen, USA). PCR amplification was attempted using divergent primers for selected circular RNA candidates ...
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bioRxiv - Immunology 2021Quote: ... The cells were centrifuged for 5 minutes at 350 x g and washed 3 times with PBS before being resuspended in R buffer (a component of the Neon electroporation kit, Thermo Fisher). Cells were resuspended in R buffer at a concentration of 20 million cells per ml ...
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bioRxiv - Cancer Biology 2020Quote: ... using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies). The pCS2:runx1 probe was a gift from Leonard Zon ...
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bioRxiv - Immunology 2021Quote: CASP1-/- and NLRP1-/- N/TERT-1 keratinocyte cells were prepared by delivery of Cas9 ribonucleoprotein complexes containing an Alt-R CRISPR-Cas9 sgRNA and recombinant Cas9 (IDT) using the Neon Transfection System (ThermoFisher Scientific) following the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2021Quote: ... were directly added to yield 50 μL PURE system reaction mixtures [28] containing 13C6 15N4 L-Arginine (R*) and 13C6 L-Lysine (K*) (Thermo Scientific) as a substitute for non-labeled L-Arginine and L-Lysine ...
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bioRxiv - Cell Biology 2021Quote: ... and the remaining collagen layer was fixed with 4% formaldehyde in PBS and stained with R-250 Coomassie Brilliant Blue (Thermo Fisher). Representative images were acquired using the 10X dry lens (NA = 0.3 ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... single cells were re-suspended at a density of 1-2 × 107 and subjected to magnetic sorting to exclude immune cells using rat-anti-mouse CD45 (R&D, MAB114) and CD24 (eBioscience, clone M1/69) magnetic sheep-anti-rat Ig Dynabeads (Invitrogen, #11035). FACS for FITC+/RFP-/DAPI-was used to obtain GFP+ve CAFs and to exclude RFP+ve tumour cells and dead cells ...
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bioRxiv - Biochemistry 2021Quote: ... Deglycosylation on the standard proteins was analyzed by gel electrophoresis on 15% SDS-PAGE gels and the gels stained by Coomassie Brilliant Blue R-250 Dye (Cat. 20278, Thermo Scientific). The optimal conditions were applied to study limited deglycosylation on LLC-MK2 cellular glycoproteins upon induction of ER stress ...
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bioRxiv - Plant Biology 2021Quote: ... the full-length coding region of GmRAV was amplified by PCR using GmRAV-TOPO-F and GmRAV-TOPO-R primers (Table S2) and cloned into pENTR/D-TOPO (Life technologies) and then transferred to the expression vector pDEST15 vector through LR reaction to generate the GST-GmRAV fusion vector ...
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bioRxiv - Microbiology 2020Quote: ... the beads were incubated for 10min at 700 rpm in the dark with 50 μl of Streptavidin-R-Phycoerythrin (Life technologies) diluted to 4 μg/mL in assay buffer ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were washed and incubated with an HRP-conjugated secondary antibody (anti-mouse, 1:5,000, 31430; anti-rabbit, 1:5,000, 31460; anti-goat, 1:5,000, R-21459, Invitrogen, Grand Island, NY, USA). The membranes were then washed again ...
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bioRxiv - Cancer Biology 2021Quote: ... RAW 264.7 cells were stimulated with the ID8 cell-derived CM supplemented with 5 μg/mL of IL-18 neutralizing antibody (R&D, Minneapolis, MN, USA) or 5 μg/mL of rat IgG1 Isotype as a control (Thermo-Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... and a mix of amino-acids (1X MEM Non-Essential Amino Acids and 1X MEM Amino Acids, both manufactured by Gibco R). For strains and plasmid construction cells were grown in LB ...
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bioRxiv - Molecular Biology 2022Quote: ... or LbaCas12a proteins were complexed with the desired crRNA at a molar ratio of 1:2.5 in Neon R buffer (Thermo Fisher Scientific) and incubated at RT for 15 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... these ORFs were inserted into the BiFC destination vector pDEST-VYNE(R)GW using the Gateway LR Clonase II enzyme mix (Life Technologies). For MSL10 ...
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bioRxiv - Neuroscience 2021Quote: ... a cell pellet containing 1 x 104 cells was resuspended in 10 μl of buffer R (Thermo Fisher Scientific, Waltham, MA) and DRG neurons were then electroporated with miR-544-3p mimic (50 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... Files containing all spectral counts for each sample and its replicates were imported into the R software from the result tables of Proteome Discoverer (v1.4, Thermo Fisher Scientific). Data were assembled into a matrix of spectral counts ...
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bioRxiv - Neuroscience 2021Quote: ... The double phosphosite mutant construct pME:kitbY568F/Y570F was made by PCR using pME:kitb as a template and the kitb 568/570Y-F IVA F and R primers with the Phusion High-Fidelity PCR Kit (ThermoFisher Scientific) followed by in vitro assembly in Top10 competent cells (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... African green monkey kidney epithelial Vero E6 cells (provided by R. Baric) were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco/ThermoFisher, Waltham, MA, USA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Orexin A/Hypocretin-1 (1:1000, R and D Systems, MAB763) Phospho-JUND (Ser255) (1:100, Thermo Fisher Scientific, PA5-104821), Phospho-nNOS (Ser1417 ...
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bioRxiv - Cancer Biology 2022Quote: ... were combined at a 1:9 ratio and then added to luciferase-stable JeKo-1 cells suspended in Buffer R (Neon Transfection System, Thermo Fisher). The suspension was pulse electroporated at 1550 volts for 30 milliseconds ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... content mixing FRET reporters were then added (500 µL of 4 mg/mL solution of R-phycoerythrin-biotin conjugate, or 296 µL of 2mg/mL Alexa-Streptavidin; both reagents from Thermo/Molecular Probes). SNARE stocks in SNARE elution buffer with β-OG were then added to a final molar ratio of 1:600 (each Q-SNARE ...
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bioRxiv - Physiology 2021Quote: ... laevis expression construct using pNB1uf/r primers (Supplementary Table 5) and cRNA was synthesized using the mMESSAGE mMACHINE™ T7 Transcription Kit (Invitrogen) according to the manufacturer’s manual.
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bioRxiv - Microbiology 2020Quote: ... BHK21 and Vero R cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) 4.5 g/L glucose (Gibco, Thermo Fisher Scientific Inc.) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... complexes were delivered into Jurkat cells using the Alt-R CRISPR-Cas9 system (IDT) and the Neon Transfection System (Thermo Fisher). Guide-RNA sequences were designed using the Broad Institute’s online sgRNA design tool and CRISPETa paired guide designer68 ...
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bioRxiv - Developmental Biology 2021Quote: ... was conjugated to AlexaFluor-488, and anti FOXC2 (Goat, R&D AF6989) was conjugated to AlexaFluor-647 using Molecular Probes Antibody Labelling Kit (Invitrogen/ ThermoFisher). When labelling OTX2 ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Genetics 2020Quote: ... mouse cDNA was amplified with primers (F: 5-gtttatgggcctcaacctcatg-3, R: 5-caggcttcactccagctttttgg-3) and then enzyme digested with BsiEI (Thermofisher #FD0894). Genotyping result using this method is shown in Fig ...
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bioRxiv - Genomics 2020Quote: ... 5′-CAGACTCAACCTCCTCAGG-3′-R) in a total volume of 20 µl of Luminaris HiGreen High ROX qPCR Master Mix (Thermo Scientific) in a real-time thermal cycler STEPOnePlus (A&B Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... 1.5 million SH-SY5Y cells stably overexpressing human A53T mutated α-synuclein (Danzer et al., 2007) were resuspended in electroporation buffer R (NEON electroporation system, ThermoFisher) and added to the antibody-fibril preparation (final volume 100 μl) ...
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bioRxiv - Immunology 2023Quote: ... 1 × 105 cells were then electroporated with 2μg Cas9 protein and 400 ng gRNA in 10 μl of resuspension buffer R (and 50 pmol of donor HDR templates in case of KI) using Neon transfection system (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... AffiniPure goat anti-mouse IgG(H+L) (JacksonImmuno Research Laboratories Inc.; 115-025-003) and AlexaFluorTM647-R-phycoerythtin goat anti-rabbit IgG(H+L) (A20991,Thermo Fisher)) ...
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bioRxiv - Cancer Biology 2022Quote: ... and transfected with 1 μg of Edit-R SMART Cas9_mCMV_(PuroR) expression plasmid (U-005200-120, Dharmacon) using 5 μL of Lipofectamine 2000 (11668027, Thermo Fisher Scientific) in 400 μL of Opti-MEM (31985062 ...
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bioRxiv - Biochemistry 2024Quote: ... were applied onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of 1.1 mg/mL or 0.5 mg/mL of complex were loaded onto freshly glow discharged R 2/2 UltrAuFoil grids84 prior to plunge freezing using a Vitrobot Mark IV (ThermoFisher Scientific) with a blot force of 0 and 6 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Neuroscience 2024Quote: ... Densitometry was performed as previously described [66] using total protein stained with 0.1% Coomassie Brilliant Blue R-250 (Thermo Fisher, 20278) as the loading control [95] ...
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bioRxiv - Bioengineering 2024Quote: A volume of 4 μl of Cy5-labeled DO structures at 50 nM concentration were mixed with 6 μl R Buffer solution (Neon kits-MPK1096 Thermo Fisher). DO structures in R buffer were then immediately subjected to electroporation using the Neon Transfection system (MPK5000 ...
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bioRxiv - Plant Biology 2023Quote: ... A total of 395 bp was amplified using specific primers (zma-394b-F/zma-mir394b-R; Table S1) and cloned into pCR8-GW vector (Thermo Fisher). The amplified region includes the annotated 122 bp hairpin (GRMZM5G871952 ...
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bioRxiv - Systems Biology 2024Quote: ... domain-focused sgRNA library (gift from Dr. Christopher R. Vakoc to to Dr. Zhiguo Zhang) was amplified using ElectroMAX Stbl4 Competent Cells (Invitrogen #11635018) according to manufacturer’s instructions with a representation > 1,000 and used to produce lentivirus as described above ...
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bioRxiv - Microbiology 2023Quote: 3 μL of 3 mg/mL purified Umb1 particle samples were loaded onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a Vitrobot Mark IV (ThermoFisher Scientific) with a blot force of 0 and 6 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Molecular Biology 2023Quote: ... frozen explants were cut in small pieces of about 0.5 mm with a scalper while kept on ice and introduced in tissue grinding CKMix50-R 2 ml tubes (Bertin Technologies) containing 500 µl of TRIzol™ reagent (Invitrogen). Tubes were placed in a Precellys Evolution tissue homogenizer (Bertin Technologies ...