Labshake search
Citations for Thermo Fisher :
151 - 200 of 631 citations for R + SCH 23390 hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... and Qubit(r) DNA HS Assay kit (Invitrogen; 32854).
-
bioRxiv - Cell Biology 2021Quote: ... and resuspended in R buffer (Neon Transfection System, Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... and 4 μM R-phycoerythrin biotin-XX conjugate (Invitrogen). Rehydrated lipids for V-liposomes were mixed with protein and dye to get a final concentration of 4 mM lipid ...
-
bioRxiv - Neuroscience 2021Quote: ... and 1X Defined Trypsin Inhibitor (Gibco R-007-100) and expanded for numerous passages and banked in NSC freezing media (NSC media + 10% DMSO) ...
-
bioRxiv - Microbiology 2021Quote: ... and Streptavidin-R-Phycoerythrin (4 μg/ml) (Life technologies). Measurements were performed using a Magpix instrument (Luminex) ...
-
bioRxiv - Systems Biology 2020Quote: ... SiteClick R-PE Antibody Labeling Kit (Life Technologies S10467) was used to conjugate the antibodies with 10-20 equivalent of 5’ DBCO-modified 18-nt DNA oligonucleotides (IDT) ...
-
bioRxiv - Microbiology 2021Quote: ... and Streptavidin-R-Phycoerythrin (4 μg/ml) (Life technologies) on an orbital shaker and protected from the light ...
-
bioRxiv - Immunology 2022Quote: ... and streptavidin R-PE conjugate (Invitrogen, Thermo Fisher Scientific). Cells were then washed and resuspended in FACS buffer ...
-
bioRxiv - Immunology 2022Quote: ... and streptavidin R-PE conjugate (Invitrogen, Thermo Fisher Scientific). Cells were then washed and resuspended in FACS buffer ...
-
bioRxiv - Systems Biology 2021Quote: ... SiteClick R-PE Antibody Labeling Kit (Life Technologies S10467) was also used to crosslink 5’ DBCO-modified 18-nt DNA oligonucleotides (Integrated DNA Technologies ...
-
bioRxiv - Biochemistry 2021Quote: ... for nicking with Nt.BspQI or 1 × Buffer R (ThermoFisher) for nicking with Nb.Bpu101) ...
-
bioRxiv - Immunology 2023Quote: ... and resuspended in 200 µL Buffer R (Thermo Fisher). 15 µg of the forward-targeting gRNA plasmid and 15 µg of the reverse-targeting gRNA plasmid were added to the Buffer R-cell suspension ...
-
bioRxiv - Immunology 2023Quote: ... and resuspended in 200 µL Buffer R (Thermo Fisher). 30 µg of maxi-prepped DNA (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... and resuspended in electroporation buffer R (Thermo Fisher Scientific). Recombinant protein was added to a final concentration of 7 μM in the electroporation slurry and electroporated by applying two consecutive 35-ms pulses with an amplitude of 1000 V ...
-
bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were resuspended in buffer R (Thermo Fisher), along with the RNP complex and ssODN template ...
-
bioRxiv - Genomics 2023Quote: ... and a Qbit(R) Fluorometric Quantification Kit (Life Technologies). All the samples had an RNA integrity number (RIN ...
-
bioRxiv - Neuroscience 2023Quote: ... PDGFRβ (AF1042, R&D Systems; 14-1402-82, ThermoFisher), MYH11 (ab224804 ...
-
STAT5B SH2 variants disrupt mammary enhancers and the stability of genetic programs during pregnancybioRxiv - Genetics 2024Quote: ... STAT5B (R&D systems, AF1584; ThermoFisher scientific, 13-5300), H3K27ac (Abcam ...
-
bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies 1:400 G α R Cy3 (ThermoFisher) incubated for 1.5 hours at RT ...
-
bioRxiv - Biochemistry 2024Quote: ... or Coomassie Brilliant Blue R-250 dye (ThermoFisher Scientific).
-
bioRxiv - Bioengineering 2024Quote: ... α-SMA (Invitrogen 14-9760-82, R&D Systems MAB1420), and BCRP (Santa Cruz sc-18841) ...
-
bioRxiv - Genomics 2021Quote: ... with protease inhibitor cocktail (Leupeptin, Carl Roth, CN33.4; Pepstatin A, Carl Roth, 2936.3; PMSF, Carl Roth, 6367.3; Benzamidine hydrochloride, Acros Organics, 105245000, resuspended in ethanol) and 250 U/μL benzonase (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: Total RNA of cells was extracted after treatment of cells with sertraline hydrochloride for 72 h using TRIzol reagent (Cat no. 15596-018, Ambion, Life Technologies, USA). Complementary DNA (cDNA ...
-
bioRxiv - Immunology 2020Quote: ... was added and incubation continued 24 hours before changing the media with Screening media (DMEM+10% FBS containing 10 μg/ml Blasticidin S hydrochloride (Invitrogen, cat#21001)) ...
-
bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
-
bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
-
bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
-
bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
-
bioRxiv - Immunology 2024Quote: ... and activated by adding 50 mg/ml sulfo-NHS (N-hydroxysulfosuccinimide) and EDC [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride] (Thermo Fisher Scientific). Each bead region was then incubated with 6.25 μg of the respective antigen yielding a final concentration of 6.25μg antigen/1 × 10^6 beads in coupling buffer [0.5 M 2-(N-morpholino ...
-
bioRxiv - Immunology 2024Quote: ... mouse and human monoclonal antibodies were partially reduced using 5 molar excess Tris(2-carboxyethyl) phosphine hydrochloride (TCEP, Thermo Fisher Scientific, #77720) for 2 hours at 37°C with rotation ...
-
bioRxiv - Cancer Biology 2021Quote: ... or Alexa flour 488 Goat Anti R (Invitrogen, Cat # A11070) secondary antibodies ...
-
bioRxiv - Developmental Biology 2021Quote: ... supplemented with 20ng/ml bFGF (R&D) on CELLstart (Gibco) coated wells ...
-
bioRxiv - Biophysics 2021Quote: ... Gels were stained with Coomassie Brilliant Blue R-250 (ThermoFisher) and scanned on a Typhoon imager (Cytiva ...
-
bioRxiv - Neuroscience 2020Quote: ... from Invitrogen or Coomassie brilliant blue R-250 dye (20278) from ThermoFisher Scientific using the following protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were incubated in buffer-R (included in Invitrogen, #MPK1025) with 2 μg/μL each of plasmids ...
-
bioRxiv - Cancer Biology 2022Quote: ... The RNPs were then resuspended in R buffer (Invitrogen, MPK1025) and transferred to pelleted cells ...
-
bioRxiv - Developmental Biology 2022Quote: ... they were pelleted and suspended in buffer R (ThermoFisher, MPK1025) at a density of 1*107 cells/ml ...
-
bioRxiv - Genetics 2022Quote: ... with 100µL reaction volume of resuspension (R) buffer (Thermo Fisher), mixed respectively with CRISPR plasmids and targeting donor plasmids ...
-
bioRxiv - Neuroscience 2020Quote: ... from Invitrogen or Coomassie brilliant blue R-250 dye (20278) from ThermoFisher Scientific using the following protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... using a Sorvall tabletop centrifuge (model St16-R-Thermo Scientific). The supernatant was discarded and a new solution of 1M hydrochloric acid was added maintaining the proportion 40mL acid/1gram shell followed by an overnight incubation at ambient temperature ...
-
bioRxiv - Microbiology 2022Quote: ... and with 200 ng mL−1 RNase R (Thermo Scientific) in 2×SSC (300 mM NaCl ...
-
bioRxiv - Cell Biology 2023Quote: ... and resuspended in a minimum amount of buffer R (Invitrogen) with a total of 1μg of DNA per electroporation reaction ...
-
bioRxiv - Cell Biology 2023Quote: ... and rabbit anti-goat (ThermoFisher Scientific, R-21459, 1:1000) were used for this study ...
-
bioRxiv - Bioengineering 2023Quote: ... Human FLRT2 antibody (#AF2877, Invitrogen R&D Systems, Minneapolis, MN) was added at 20 μg/mL in 100 μl stain buffer per 1x106 cells and incubated for 20 min at room temperature protected from light ...
-
bioRxiv - Microbiology 2023Quote: ... and electroporated in buffer R (Neon transfection reagent; Invitrogen, USA) with 3 µg of the reporter plasmid pUCHR-EF1a-inNluc ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were electroporated in R Buffer (Thermo Fisher Scientific, BR5) containing 150 ng/µl sgRNA and 500 ng/ul recombinant TrueCut Cas9 protein (Thermo Fisher ...
-
bioRxiv - Immunology 2024Quote: ... cloned into TOPO-PCR4 (Thermo Fisher Scientific, catalog #r K458001) and individual colonies were sequenced ...
-
bioRxiv - Cancer Biology 2024Quote: ... SYBR (R) GREEN JUMPSTART TAQ Ready mix (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2022Quote: ... PAFB was labelled with the green fluorophore 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylenediamine hydrochloride (BODIPY™ FL EDA, Invitrogen, Waltham, MA, USA) as described (32).