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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Dengue Virus Serotype 2 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... or with Protein A agarose beads incubated with 2 μg anti-LONP1 antibody (ThermoFisher Scientific 15440-1-AP) overnight (16 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... the input aliquot (100 µl) was mixed with equal volume of 2× protein sample buffer (2× NuPAGE LDS buffer [Life Technologies], 100 mM DTT, 4% [w/v] SDS) and the IP with 100μl 1 x protein sample buffer followed by incubation for 10 min at 75 °C ...
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bioRxiv - Genetics 2021Quote: ... protein transfer to a nitrocellulose membrane was completed using the iBlot 2 Dry Blotting System (Thermo Fisher Scientific Scientific). The membrane was then blocked for one hour at room temperature using a 5% milk solution in 1× TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... were resolved on precast NuPAGE 4-12% Bis-Tris midi 12+2-well protein gels (cat. no. WG1401BOX, Invitrogen) at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the transferring of resolved proteins onto PVDF membranes via an iBlot 2 Dry Blotting System (ThermoFisher Scientific). Following blocking for 60 min with 1% BSA in TBST (10 mM Tris-HCl (pH7.4) ...
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bioRxiv - Neuroscience 2019Quote: ... An equal amount of protein per sample was loaded into 2-12% gradient NuPage Bis-Tris gels (Thermo Scientific) and protein was separated by electrophoresis ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S protein Omicron genes were synthesized and inserted into pcDNA3.1 (GeneArt Gene Synthesis, Thermo Fisher Scientific). Pseudotyped retroviral particles were produced in HEK293T cells as described previously (32) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then initially stained with microtubule-associated protein 2 (MAP2) monoclonal primary antibody (M13) (ThermoFisher Scientific,ON, Canada) at a 1:300 dilution then incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Electrophoresis was performed and proteins were dry transferred to a nitrocellulose membrane using an iBlot 2 system (ThermoFisher Scientific). The blots were probed with anti-phospho-AKT (Ser473 ...
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bioRxiv - Biochemistry 2020Quote: ... Binding of the full-length S protein was detected by mouse anti-His IgG (Invitrogen MA121315, 2 μg/ml) and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001 ...
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bioRxiv - Biochemistry 2020Quote: StNFeoB proteins (2-5 µM each) were incubated with a range of substrate (GTP for molecular biology, Thermo Scientific) concentrations (25 µM to 5000 µM) ...
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bioRxiv - Cell Biology 2021Quote: ... Bound proteins were eluted by boiling in 25 μL of 2×LDS sample buffer (NuPAGE LDS Sample Buffer, Invitrogen). Anti-Protein A Western blot was performed to detect sumoylated Mcm21 or Ctf3 species in the purified material ...
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bioRxiv - Biochemistry 2022Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in 1xPBS and probed with the anti-mono-ADP-ribose binding reagent/antibody MABE1076 (α-MAR) ...
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bioRxiv - Genomics 2022Quote: ... diluted chromatin was incubated for 2 h with antibodies (2.5 μg/100 μL) coupled to magnetic Dynabeads Protein A (Invitrogen). ChIP samples were washed 4 times in ice-cold RIPA buffer ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
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bioRxiv - Developmental Biology 2019Quote: ... Lysates were then thawed on ice and 2 μL were used to perform a BCA protein assay (ThermoFisher, # 23225). 10 μL of lysate were diluted 1:1 in 1X MSD lysis buffer and added to the 96-well Mesoscale assay plate ...
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bioRxiv - Microbiology 2021Quote: ... TATA-box binding protein (TBP) (Hs00427620_m1) and beta-2-microglobulin (B2M) (Hs00187842_m1) was used (all from Thermo Fisher Scientific). PCR was performed using the LightCycler 480 Instrument (Roche Life Science ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein (kindly provided by Eric Fischer, DFCI) was coupled to fluorescent streptavidin beads (Thermo Fisher) and incubated with diluted plasma samples to allow antibody binding to occur ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transiently transfected with 2μg of either wild-type or mutant SARS-CoV-2 S protein plasmid with Lipofectamine 3000 (Invitrogen) at a ratio of 1:2:2 DNA ...
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bioRxiv - Biochemistry 2020Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in PBS containing 0.05% Tween-20 and probed with anti-mono or poly ADP-ribose binding reagents/antibodies MABE1076 (α-MAR) ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein-antibody complex was developed by adding 2-3ml of SuperSignal West Pico Chemiluminiscent substrate (Thermo Fisher, USA).
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bioRxiv - Plant Biology 2022Quote: Protein samples were reduced with Tris(2-carboxyethyl)phosphine (TCEP) and cysteines alkylated with Iodoacetamide (IAA, Thermo Fisher, Germany). Subsequent proteolytic digests were performed using S-TRAPs (Protifi ...
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bioRxiv - Microbiology 2022Quote: ... COS-7 cells grown on 13 mm cover slips were transfected with either the empty pcDNA3.1(+) vector or one expressing the SARS-CoV-2 E protein using Turbofect transfection reagent (ThermoFisher). At 24 h pt ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred on nitrocellulose membranes using the iBlot 2 Dry Blotting System (Invitrogen, Cat# IB21001 and IB23001). The high molecular weight protein GCN1 was transferred using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... Equivalent amounts of protein (∼2 mg) were used for immunoprecipitation with Pierce anti-c-Myc Agarose (Thermo Scientific, 20169), anti-Flag Affinity Gel (Bimake ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto a nitrocellulose membrane using iBlot 2 gel transfer device (7 min at 25 V, ThermoFisher). Membranes were incubated for 1 hour at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... and the insect cell codon-optimized RBD at residues 330 to 521 of SARS-CoV-2 S protein (GenBank accession no. MN908947.3) was cloned into a pFastBac plasmid (Invitrogen). For the RBD-LTA fusion protein expression ...
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bioRxiv - Neuroscience 2023Quote: ... were incubated for 2 h at RT and the proteins were detected with Supersignal West Pico (34580, Thermo Scientific) and imaged by using iBright 1500 (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... 2 µg/ml spike protein diluted in PBS was incubated over-night on 96-well high affinity plates (Nunc). To prevent unspecific binding ...
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bioRxiv - Cancer Biology 2023Quote: ... Oxidized proteins were visualized using the Thermo Scientific Pierce ECL 2 Western Blotting Substrate (Thermo Fisher Scientific, Cat. 80196) and scanned ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2023Quote: ... 2/3rd of the eluted protein samples were resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and subjected to Silverstein (Pierce™ Silver Stain Kit ...
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bioRxiv - Bioengineering 2023Quote: ... FAM-protein conjugates were then purified with Zeba 2 mL spin desalting columns with 40 kDa MWCO (Thermo Scientific) to remove excess unreacted FAM-NHS according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 2 ug of EV protein content were separated on a 4-12% Bis-Tris gel (Thermo Scientific, Rockford, IL), then blotted onto a PVDF membrane ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... For the SARS-CoV-2 staining a polyclonal rabbit anti-SARS Coronavirus Spike protein (PA1-41142, Thermo Fisher, France) and revealed using an anti-rabbit IgG (H+L ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.01% [v/v] 2-mercaptoethanol) and then quantified with the Pierce BCA protein assay kit (Thermo Fisher Scientific). The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in PBS containing 0.05% Tween-20 and probed with anti-mono ADP-ribose binding reagent MABE1076 (α-MAR ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The immuno-detection of proteins was carried out using mouse anti-HA monoclonal antibody (2-2.2.14, #26183, ThermoFisher Sci) and secondary anti-mouse IgG antibodies conjugated with horseradish peroxidase (#A9044 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation was performed by adding 2 mg of P-CREB antibody to protein A/G Dynabeads (Life Technologies, USA) and incubating the mixture overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: Concentrated virus samples were mixed with Novex™ Tris-Glycine SDS Sample Buffer (2X) (Thermo Fisher Scientific) with NuPAGE™ Sample Reducing Agent (10X ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with virus containing scrambled control or targeting MTR and Polybrene (8 ug/mL, Invitrogen). Cells were split after 48 h into standard RPMI-media (10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... the virus solution was typically mixed at 1:500 with blue beads (fluorescent polymer microspheres, Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Reovirus S4 RNA was quantified using the Taq-Man Fast Virus One-Step Master Mix (Applied Biosystems) and GAPDH was detected as an endogenous control using the Pre-Developed TaqMan Assay Reagent kit for mouse GAPDH (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Biochemistry 2021Quote: ... high titer virus generation (V1) was then used to infect 1200 ml High Five™ cells (Invitrogen) for large scale protein production ...
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bioRxiv - Microbiology 2020Quote: ... centrifuged and suspended in virus media that is composed of DMEM high glucose (Thermo Fisher Scientific, USA), with 2% fetal bovine serum (Thermo Fisher Scientific ...