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Citations for Cell Signaling Technology :
2401 - 2450 of 10000+ citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated for 48h at 4°C with the following primary antibodies: rabbit anti-phospho-S6 (1:1000, Cell Signaling, Cat# 5364), mouse anti-NeuN (1:400 ...
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bioRxiv - Pathology 2021Quote: ... and incubated with primary antibodies at 4°C overnight at the following concentrations: β-catenin (Cell Signaling, 8480, 1:1000 in 1% BSA), p65 (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies for western blots were diluted as follows: rabbit anti-Phospho-AKT Ser473 (D9E) (Cell Signaling Technology, USA; RRID: AB_2797780; 1:2000), mouse anti-Pan-AKT (40D4 ...
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and blotted at 4°C overnight with primary antibodies as follows: rabbit anti-phospho-GSK-3β (Ser9) (5B3) (1/500 dilution; Cell Signaling; cat #9323), rabbit anti-GSK-3β (1/1000 dilution ...
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bioRxiv - Physiology 2019Quote: ... blocked with 5% non-fat powdered milk in TTBS solution and incubated with primary antibodies from Cell Signaling (1:1000; Danvers, MA, USA) against p-IGF1RβY1135 (3918) ...
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bioRxiv - Cell Biology 2019Quote: ... blocked for an hour with 5% BSA-Tris Buffered Saline (TBS) and probed with primary antibodies anti-HA (C29F4) Rabbit monoclonal antibody (mAb) (Cell Signaling Technology®) or mouse raised anti-GFP antibodies (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were incubated overnight with one of the following rabbit primary antibodies (all from Cell Signaling Technology and all cross-react with rat): anti-Pan-AKT (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% skimmed milk and incubated with primary antibodies (rabbit anti-PPAR-γ antibody, Cell Signaling Technology, 1:1000) and HRP-conjugated goat anti-rabbit IgG ...
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bioRxiv - Molecular Biology 2019Quote: ... UK with catalogue numbers ab52908 and ab76227 respectively and housekeeping mouse raised β-tubulin (51 kDa) primary antibody was procured from Cell Signaling Technologies, MA ...
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bioRxiv - Cell Biology 2019Quote: ... Blotted nitrocellulose membranes were incubated with primary antibodies (mouse -p53, SC-126, Santa Cruz Biotechnology; rabbit -CTCF, Millipore 07-729; rabbit -GAPDH, cst-2118, Cell Signaling Technology) followed by detection with HRP conjugated secondary antibodies (Jackson Immunoresearch Laboratories) ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were blocked at room temperature with 1% BSA for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit Collagen I antibody (Cell Signaling Technology, 84336), mouse E-cadherin antibody (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... using heat-induced antigen retrieval at pH 9.0 for 20 minutes, followed by primary antibody (PD-1, clone EH33; dilution 1:200) (Cell Signaling, Beverly, MA, USA) incubation for 15 minutes ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... and blocked with 5% skimmed milk in TBST for 1 h followed by O/N incubation at 4 °C with appropriate primary antibody: pSTAT3 (D3A7; Cell Signaling Technology, 9145S) and STAT3 (124H6 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... The slides were incubated overnight with the following primary antibodies at the indicated dilutions: rabbit monoclonal anti-HA 1:1000 (Cell Signaling Technology 3724), rabbit polyclonal anti-PTEN 1:400 (Thermo Fisher 51-2400) ...
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bioRxiv - Biochemistry 2022Quote: ... phosphorylated at Ser293 PDHA and acetylated proteins were estimated by western-blotting using primary antibodies from Cell Signaling Technology (Danvers, MA, United States) with the product numbers #5490 ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with hematoxylin-eosin staining for 10min or overnight at 4℃ using the following primary antibodies: β-catenin (1:200, #8480, Cell Signaling Technology), Ki67 (1:400 ...
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bioRxiv - Plant Biology 2022Quote: ... membranes were incubated overnight at 4°C with the primary antibody in 1 % milk solution in TBS (phospho-AMPK, 1:2000, Cell Signaling Technology, #2535). The next day ...
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bioRxiv - Plant Biology 2022Quote: ... was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H+L)-HRP conjugate ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 2 hours or overnight at 4°C with the following primary antibodies: Sirt3 (1:200, Cell Signaling Technology 5490S), TOMM20 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... To detect changes in IL-6 signal transduction we used primary monoclonal rabbit anti-Stat3α (D1A5) from Cell Signaling Technologies (Cat no. 8768S) and used the same secondary anti-rabbit antibodies described above.
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were subsequently blocked with 5% milk or 5% bovine serum albumin for 1 h at room temperature and probed separately with specific primary antibodies to AKT1 (1:1,000 dilution, 75692, Cell Signaling Technology, Danvers, MA), pan-AKT (1:1,000 dilution ...
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bioRxiv - Physiology 2022Quote: ... for 1 hour at RT and then incubated O/N with primary antibody for c-Fos (Rabbit anti-cFos, Cell Signaling, Cat# 2250) in blocking solution at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using goat anti-mouse or anti-rabbit IgG (H+L) Alexa fluor 488 or 647 (Cell signaling Technologies/ Bioconcept) secondary antibodies diluted 1:500 in 1% BSA 0.1% Triton-X 100 in PBS for 3 hours in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... β-actin [8H10D10] primary antibodies and anti-rabbit-HRP or anti-mouse-HRP antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). IRF3 [A19717] were purchased from ABclonal (Woburn ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: anti-AMPKα (Cell Signaling Technology, USA; diluted at 1:1000), anti-p- AMPKα (Thr172 ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were washed and then blocked using 1% Goat serum in TBS for one hour at room temperature before adding primary antibodies to detect Cd45 (Cell Signaling, 1:500) and Cd11b (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: All cargo constructs include an epitope tag or tandem tag (FLAG or 3xFLAG) for assay by anti-FLAG primary antibodies (Cell Signaling Technology, 2368). HEK293FT cells were transfected at 24 well ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed twice with PBS-T (0.01% Tween) and incubated with primary antibodies (anti-PARP1 antibody, Abcam ab227244; anti-MSH2 antibody, Abcam ab52266; anti-RPA1 antibody, Cell Signaling Technology 2267) in DMEM + 10% FBS (alternatively add 3% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... cells were permeabilized in 0.25% Triton X-100 in PBS for 5 minutes and blocked in 10% goat serum/0.1% Triton X-100 in PBS for 30 minutes and then placed in the primary antibody overnight at RT (γH2AX, Cell Signaling, 1:500). The next morning ...
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bioRxiv - Cell Biology 2022Quote: ... the following primary antibodies were used: rabbit polyclonal anti-phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) at 1:250 (Cell Signaling Technology, #3141), rabbit polyclonal anti-phospho-Myosin Light Chain 2 (Ser19 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... The processed tissue was rinsed 3 times with PBS (20min each) to wash off the Dent’s Fix and then incubated with primary antibody in blocking buffer (pS6 Cell Signaling 2215, 1:1000) at 4°C with gentle rotation for 5 days ...
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bioRxiv - Neuroscience 2023Quote: ... containing 5% bovine serum albumin (BSA) for 30 min and then incubated with primary antibodies against p-ERK (#4370S, 1:1000, Cell Signaling, Danvers, MA), p-P38 (#4511S ...
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bioRxiv - Cancer Biology 2023Quote: ... These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology, USA) secondary antibodies conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% BSA in TBST for 1.5 hours and primary antibodies were added overnight at the following concentrations: anti-PARP1 (Cell Signaling Technology, 1:1000), anti-PAR (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS−/−] at room temperature for 1.5 h and subsequently incubated with primary rabbit anti-MPO antibody (Cell Signaling, clone: E1E7I, 1:500) and primary mouse anti-HLA-G antibody (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-rat secondary antibody was used for detection of primary antibodies and visualized by SignalStain DAB Substrate kit (Cell Signaling Technology, Danvers, MA). All stained tissues were blindly evaluated by pathologists ...
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bioRxiv - Cancer Biology 2023Quote: ... and blocked in room temperature with 5% BSA (HiMedia, India) solution for an hour and incubated with respective primary antibodies [FOXM1 (1:2000) (Cell Signaling Technologies, USA); active + pro Caspase-3 (1:3000 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in 5% BSA with 1% Tween 20 for 1 hour then probed overnight at 4°C with the following primary antibodies at a 1:1,000 dilution: Phospho-STAT1 (Cell Signaling Technologies, 9167S, monoclonal), SOCS1 (Cell Signaling Technologies ...
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bioRxiv - Genetics 2022Quote: ... Neurons were subsequently blocked with 5% normal goat serum in 1x PBS for 1 h and then incubated with primary antibodies as follows: anti-GFP (Cell signaling, #2555S, R), anti-Gephyrin (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked in 5% skimmed milk and incubated overnight at 4°C with primary antibodies for UTX (Cell Signaling Technologies #33510), GFP (JL-8 ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was blocked for 1 h at room temperature with 5 % BSA dissolved in TBS-Tween (0.1 %) and incubated with the primary antibody rabbit anti-phospho-threonine [(Cell Signaling Technology®, #9381) 1:1000] ...
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bioRxiv - Biochemistry 2023Quote: ... The bound primary antibodies were detected with an HRP-conjugated anti-mouse immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology, Catalog# 7076S) by chemiluminescence (VWR) ...
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bioRxiv - Physiology 2023Quote: ... was heated at 90°C for 5 minutes and loaded into acrylamide gels before incubated with primary antibodies against catalase (CAT, #14097, Cell Signaling, 1:5000), 3-nitrotyrosine (3-NT ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with the primary antibody mAb c-MYC (dilution 1:1000 in TBST/5% milk; Cell Signaling Technology, D84C12). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were blocked with TBST/5% Normal Goat Serum for 1 hour at RT before overnight incubation with primary antibody diluted in Diluent CST (Cell Signaling Technology, 8112) at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were incubated overnight with primary monoclonal anti-Myosin IIa (clone E7Y9O) at the recommended concentration in blocker (Cell Signaling Technology, MA, USA) followed by anti-rabbit IgG HRP linked secondary antibody at 1:10,000 in TBS-T for 1h at RT (antibodies were from Cell Signaling Technology ...