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Citations for Cell Signaling Technology :
2251 - 2300 of 10000+ citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (#3195, 1:100 dilution, Cell Signaling Technology), anti-SOX9 rabbit antibody (#AB5535-25UG ...
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bioRxiv - Pathology 2022Quote: ... The blotted membranes were incubated with the following primary antibodies: rabbit monoclonal anti-phosphorylated Smad2 (Ser465/467) antibody (Cell Signaling Technology, #3108), rabbit monoclonal anti-Smad2 antibody (Cell Signaling Technology ...
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bioRxiv - Physiology 2022Quote: ... in tris-buffered saline with 0.1% Tween 20 detergent (TBST) ) with primary antibody at a dilution of 1:500 for Phospho-Smad2 (Ser465/467) (138D4) (Rabbit mAb, 3108, Cell signaling, USA), 1:500 Phospho-Smad3 (Ser423/425 ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight in blocking buffer at 4°C with the following primary antibodies: 1:2000 c-fos (Cell signaling technology), 1:2000 ds-red (Takara Bio) ...
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bioRxiv - Biophysics 2022Quote: ... Antigen recognition was performed by incubating primary antibody in a humidified chamber for different times (Phalloidin, Invitrogen, A12381; anti-Vinculin, Invitrogen, 42H89L44; anti-pFAK, Cell signaling, 3283S) and with anti-mouse/rabbit Alexa Fluor 488-594 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibody incubations were done in 5% milk/PBS using rabbit anti-HA at 1:500 (Cell Signaling, Cat #: 3724S) and anti-Rabbit HRP at 1:10,000 (BioRad ...
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bioRxiv - Neuroscience 2019Quote: ... Cells then underwent immunocytochemical processing similar to that described above using primary antibodies against activated c-Jun-N-terminal kinase (rabbit anti-pJNK, 1:100, Cell Signaling Technology) or phosphorylated p38 MAPK (rabbit anti-p-p38 ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were washed four times with TBST (5 minutes gentle rocking per wash) and incubated overnight with primary antibody Rabbit anti-Gli-1 (Cell signaling, #C68H3); 1:250 dilution in TBST-Milk at 4°C with gentle rocking ...
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bioRxiv - Biochemistry 2019Quote: ... After washing with TBST blots were then incubated with a 1:1000 dilution of the primary antibody (p-CRAF (S259) (Cell Signaling Technology), p-CRAF (S298/296 ...
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bioRxiv - Cell Biology 2019Quote: Western blots for quantifying the Twitch-2B concentration were probed with a primary antibody against GFP (Cell Signaling Technology, Beverly, MA; #2555); this antibody also recognizes CFP and YFP ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated at 4°C overnight with the following primary antibodies diluted in buffer G: rabbit polyclonal anti-cleaved caspase-3 (Asp175) (Cell Signaling, #9661) at 1:250 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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bioRxiv - Genetics 2020Quote: ... followed by overnight incubation with primary antibodies overnight at 4°C (anti-dS6K gift from Thomas Neufeld; dilution at 1:3500, anti-pS6KThr398 Cell Signaling Technologies, #9209 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein separation was achieved via SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting with the following primary antibody dilutions: rabbit monoclonal anti–Caveolin-1 (1:1000; Cell Signaling #3267), mouse monoclonal anti–Actin (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... the primary antibody type (rabbit) was switched to mouse by using a conformation-specific mouse anti-rabbit antibody (Cell Signaling Technologies #3678) that only recognizes non-denatured antibodies to reduce the background from the heavy and light chains of the denatured IP antibody ...
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bioRxiv - Bioengineering 2020Quote: ... and blocked with 1% BSA for 1 hour prior to incubation with primary antibodies raised against OCT4 (Cell Signaling, Cat. # 28901:800), NANOG (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... or α-actinin1 with the corresponding primary antibody and an Alexa Fluor 488-coupled secondary antibody (Cell Signaling Technology #4408, 1/500) or an Alexa Fluor 546-coupled secondary antibody (Molecular Probes A11056 ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked with 5% milk in 5% tris-buffered saline with 0.01% Tween-20 (TBST) for 1 hour at room temperature then incubated overnight with the primary antibodies anti-GAPDH (Cell Signaling Technology) and anti-IVNS1ABP (Atlas Antibodies) ...
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bioRxiv - Biochemistry 2021Quote: ... Slices were blocked with 5% donkey serum (37°C, 30 min) and then incubated with a primary antibody targeting RIPK3 (#95702, Cell Signaling Technology) at 4°C overnight diluted in antibody diluent (Dako) ...
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bioRxiv - Microbiology 2021Quote: ... The list of primary commercial Ab used in this study included: the rabbit anti-HA mAb clone C29F4 (Cell Signaling Technology, USA), the mouse anti-human mAbs CD3 clone OKT3 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Equal protein loading was verified by stripping off the original antibodies and re-probing the membranes with a different primary antibody such as anti-GAPDH (Cell Signaling Technology). Pertussis toxin (PTX ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked with 5% non-fat milk/PBS for 30 min and incubated with primary antibodies as follows: anti-PDGFRA (Cell Signaling, 3174T), anti-COL1A1 (Proteintech ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 3% BSA or 5% milk and probed with the following primary antibodies-1:1000 rabbit anti-Flag (Cell Signaling technology); 1:100 chicken anti-E.histolytica CSN5 (custom) ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were saturated and blots were probed with the following primary antibodies: 1:700 rabbit anti-βArr2 (Cell signaling Cat. No. 3857); 1:1000 rabbit anti-GSK3β (Cell signaling Cat ...
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bioRxiv - Bioengineering 2022Quote: ... sections were blocked with 3% bovine serum albumin / TBS and then incubated with primary antibodies (rabbit anti α-actinin (Cell Signaling, 3134)) overnight at 4 °C at 1:30 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with the following primary antibodies: phospho-p44/42 MAPK (EER1/2) (T202/Y204) (Cell Signaling Technology, Cat. #: 9101, 1:1000), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: anti-myosin-5a antibody (1:100, #3402, Cell Signaling Technology), or anti-myosin-6 antibody (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking the membranes were incubated cold overnight with primary antibodies diluted in Intercept (TBS) Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Endogenous peroxidase inhibition and blocking with normal horse serum was also carried out before the incubation with primary antibodies (anti-rabbit caspase3, 1:100, Cell signaling #9662), anti-mouse ki67 ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were hybridized with the following primary antibodies as indicated: TYROBP (1:500, antibody provided in collaboration with Dr Richard W. Cho at Cell Signaling Technology); AT8 anti-pTAU pSer202/Thr205 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight incubation at 4°C with the following primary antibodies: 4EBP1 (1:1,000, Cell Signaling Technology, Cambridge, UK #9644), MYCN (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... The final concentrations of the primary and secondary antibodies and blocking solutions used in the brain sections are as follows: Rabbit anti-cFos (Cell signaling 2250S), donkey anti-rabbit 647 (Jackson ImmunoResearch 711-605-152 ...
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585 ...
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bioRxiv - Bioengineering 2021Quote: ... The fixed samples were permeabilized in PBS containing 0.1% Triton X-100 and 1% Fetal Bovine Serum for 30 min at room temperature before filling the channels with staining buffer (1.5% BSA in PBS) containing primary antibodies directed against cleaved caspase-3 (Cell Signaling Technology, 9661S) or VE-cadherin (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... membrane and incubated with each antibody (primary antibody) and then with a horseradish peroxidase (HRP)-linked anti-rabbit IgG (secondary antibody; 7076, Cell Signaling Technology). Immunoreactive proteins were detected with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... slide mounted sections were incubated overnight at room temperature with a rabbit anti-cFos monoclonal primary antibody (9F6, Cell Signaling, 1:8000), diluted in 0.1M phosphate buffer at pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... The parasites were incubated for 2 hours at room temperature with the primary antibodies 1:1000 anti-HA (C29F4) Rabbit monoclonal antibody (mAb) from Cell Signaling Technology® ...
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bioRxiv - Cell Biology 2019Quote: ... samples were transferred to PVDF membranes and western blotting performed as described previously [37] using a primary antibody for phospho-p38 MAPK (#9215; Cell Signaling Technology). Membranes were re-probed with p38α antibody (#9228 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Cell Biology 2019Quote: ... Filters were incubated at 4 °C with primary antiserum diluted in 5% milk TBST as follows: FLAG antiserum (1:1000, Cell Signaling Technology), α-tubulin antiserum (1 ...
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bioRxiv - Genetics 2019Quote: ... Membranes were blocked in 5% non-fat milk and incubated overnight at 4 °C with primary antibodies: anti-GPR151 (LSBio # LS-B6760-50) or anti-beta-actin (Cell Signaling #3700). Subsequently ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were diluted in either 5% goat serum/PBS-T or SignalStain Boost IHC Detection Reagent (8114S, Cell Signaling Technology (CST)) ...
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bioRxiv - Cell Biology 2020Quote: ... Total and phosphorylated MAPK were immunoblotted with anti-mouse total and anti-mouse phosphorylated MAPK primary antibodies (Cell Signaling Technology, Danvers, MA), respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... The following primary antibodies diluted in the blocking buffer were used for staining: anti-eIF6 monoclonal antibody (D16E9) (1:300) (Cell Signaling Technology), or anti-eIF6 monoclonal antibody (1:350 ...
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bioRxiv - Physiology 2021Quote: ... A mouse primary anti-CaSR monoclonal antibody (1:2000, Gentex, Zeeland, MI) or a mouse primary anti-Myc (9B11) monoclonal antibody (1:1000, Cell Signaling Technology) was applied followed by a secondary horseradish peroxidase-coupled secondary antibody (1:5000 ...
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bioRxiv - Physiology 2021Quote: ... Sections were blocked with 5% donkey serum in the presence of 0.1% Triton followed by overnight incubation with primary antisera at 4 degrees (pCREB, Cell Signaling; Glucagon, Abcam). After extensive washing ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence staining was performed by a standard protocol using an anti-HA rabbit primary antibody (C29F4, Cell Signaling Technology, diluted 1:1600) and a secondary antibody goat anti-rabbit Alexa Fluor 633 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... MAPK activity was assessed using phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) primary antibody (1:200, rabbit, Cell Signaling Technologies #9101), total p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The neutrophils were then incubated overnight at 4°C with primary antibodies: anti-histone H3 (1:50 dilution; Cell Signaling Technology, 3680) and either anti-NE (1:100 dilution ...