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Citations for Cell Signaling Technology :
2501 - 2550 of 10000+ citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 0.15%) and respectively incubated overnight with primary and corresponding secondary antibodies [rabbit anti p-mTOR (1:1000; #5536, Cell Signaling Technology, Danvers, MA, USA); rabbit anti mTOR (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then incubated overnight with the following primary antibodies at 4°C: rabbit anti-Ki67 (1/200; 12202; Cell Signaling Technology, Danvers, MA), rabbit anti-Olfm4 (1/400 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by exposure to primary antibody (anti-Alk1 Rabbit pAb, Abcam #ab68703, 1:500; or, anti-Tubulin Rabbit Ab, Cell Signaling #2144S, 1:500) overnight in 1% BSA TBS-T at 4°C ...
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bioRxiv - Immunology 2023Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with primary anti–SYK (1:1000 dilution, Cell Signaling Technology, no. 2712S), anti–p-p38 (1:1000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibodies used were p-ser129 alpha-synuclein (FujiFilm cat# 015-25191, 1:1000) and HO-1 (Cell Signaling cat# 26416, 1:200). Sections were allowed to incubate overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated overnight at 4°C in the following primary antibodies: rabbit anti-cleaved NOTCH1 (Val 1744) at 1:1000 (Cell signaling technology, Cat #: 4147S), rabbit anti-NOTCH1 (D1E11 ...
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated with the following primary antibodies for 60 min at room temperature: anti-Cas9 (1:1000 dilution; #14697S, Cell Signaling Technology, Danvers, MA), anti-MDM2 (1:1000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated with the following primary antibodies for 2 hours at room temperature in 5% BSA: 1:1000 anti-SMARCC1 (Cell Signaling Technologies cat. #11956T), 1:1000 anti-SMARCA4 (Cell Signaling Technologies cat ...
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bioRxiv - Genetics 2023Quote: ... Cells were blocked with 5 mg/ml BSA in 1X PBS for 30 minutes and then incubated with primary antibody phospho-histone H2AX Ser139 20E3 (Cell Signaling #9718, 1:500) for 2 h at room temperature ...
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bioRxiv - Genomics 2023Quote: ... membranes were incubated overnight at 4°C with different primary antibodies in TBS-T against LEF-1 (1:500, Antibody# 2230, Cell Signaling Technology, MA, USA), and β-actin (1:30000 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with the primary antibodies (Mettl3 Abcam ab195352 1/200; N-Myc Cell Signaling Technology D1V2A 1/200). EU fluorescence coupling was performed following the manufacturer ’s instructions for Click-iT™ RNA Alexa Fluor 488 Imaging Kit ...
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bioRxiv - Biochemistry 2023Quote: ... Coverslips were then blocked with 5% BSA for 60 minutes at room temperature and incubated overnight at 4°C with primary antibody γH2AX (rabbit monoclonal; Cell Signaling 9718T, 1:400). Texas red species-specific secondary antibodies (Jackson ImmunoResearch Laboratories Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was blocked in 5% nonfat milk in PBST (PBS and 0.1% TWEEN20) and incubated overnight at 4°C with primary antibodies mouse anti-GFP Living Color (Cell Signaling Technology 2955, 1:1000) to detect TDP-43 YFP and rabbit anti-Beta-Actin (Cell Signaling Technology 4967S ...
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bioRxiv - Neuroscience 2023Quote: ... for 1h at RT and then immunolabeled with rabbit primary antibodies, anti-GIRK2 (1:500, Alomone labs, APC-006, RRID: AB_2040115) and anti-actin (1:2000, Cell Signaling Technology, 4970S, RRID: AB_2223172) overnight at 4°C in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilized with 0.5% Triton X-100 in blocking buffer for 15 min and incubated with primary antibodies against internal epitopes (DARPP32 (1:500, Cell Signaling Technology 2306, RRID:AB_823479) or PSD95 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated in the following primary antibodies diluted in blocking solution: anti-E-cadherin (24E10, CST 3195, Cell Signaling, dilution 1:200), anti-ezrin (E1281 ...
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bioRxiv - Microbiology 2023Quote: ... followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500; Cell Signaling Technology, mAb #12830) or GAPDH (1:5000 ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then blocked in 2% BSA-PBS solution for 1 hr at room temperature and then incubated with a primary antibody against β-catenin (1:100; Cell signaling technology, #9562) diluted in 2% BSA-PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% BSA diluted in TBS-Tween0,1% for 1 hour and primary antibodies were incubated overnight (rabbit anti-HER2 #29D8 Cell Signaling; mouse anti-vinculin #A250291). Donkey anti-mouse IgG or goat anti-rabbit IgG secondary antibody conjugated to horse-radish peroxidase (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Plant Biology 2024Quote: ... and MAPK activation was assessed by immunoblot analysis using primary anti-Phospho-p44/42 MAPK antibody (Erk1/I 2; Thr-202/Tyr-204) (Cell Signaling, Danvers, MA, USA) and secondary goat anti-rabbit-HRP antibody (Cell Signaling ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The tissues were then incubated overnight at 4°C with anti-DeltaFosB primary antibody (DeltaFOSB; rabbit; Cell Signaling Technology, ref. 22509; 1:1000 dilution) or anti-Fos antibody (c-Fos ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were blocked in a 5% nonfat milk solution and stained for the common EV marker CD9 using a primary antibody (Cell Signaling Technologies, Cat. # 13174) and an anti- rabbit HRP secondary antibody (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Samples probed with rabbit primary antibodies were subsequently probed with horseradish peroxidase (HRP)-linked anti-rabbit IgG (1:2500 dilution; Cell Signaling Technologies; item # 7074) secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated at 4°C overnight with the following primary antibodies: anti-phospho-MEK1/2 (Cell Signaling Technology, Inc., MA, USA, #9122), anti-MEK1/2 (Santa Cruz biotechnology ...
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bioRxiv - Systems Biology 2024Quote: ... Membranes were blocked in 5% milk or bovine serum albumin (BSA) for 1 h at room temperature and incubated with primary antibodies against: PI3 Kinase Class II α (Cell Signaling, 1:2000), Phospho-AKT(Ser473 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with anti-human N-Myc rabbit monoclonal antibody (#51705, Cell Signaling Technology, Inc., MA, USA at a dilution of 1:300) and S100 beta (EP32 ...
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bioRxiv - Bioengineering 2023Quote: ... Paraffin sections were used for H&E staining as well as sequential double-immuno-staining with rabbit anti-human Ki67 (Cell Signaling Technologies, US) and rabbit anti-human vimentin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Human CSF samples were not lysed and free and total RIPK1 levels were separately detected with (Cell Signaling Technology, 3493 and abcam, ab125072). The assay was developed with 150 mL of 2x MSD read buffer (R92TC-3 ...
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bioRxiv - Developmental Biology 2021Quote: ... Western blots were then blocked with LI-COR blocking buffer and incubated overnight with primary antibodies anti-pCREB (Ser133)(Cell Signaling 87G3, #9198, 1:1000) and anti-GAPDH (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in a solution of rabbit anti-c-fos primary antibody (1:1000 dilution, 2250T Rabbit mAb /74620 Mouse mAb, Cell Signaling Technology, Danvers, MA, USA) or mouse anti-TPH2 primary antibody (1:100 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... The same membrane that was used to detect AIRE protein was washes and reused in an incubation with an anti-GAPDH rabbit polyclonal primary antibody (Cell Signaling Technology, Beverly, MA, USA) following incubation with peroxidase conjugated anti-rabbit antibody.
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bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... Free-floating sections were blocked with 5% donkey serum for 1.5 hours before overnight incubation at 4°C with primary antibodies (goat anti-Albumin antibody, Abcam ab19194, 1:100 dilution; rabbit anti-V5 antibody, Cell Signaling Technology, 1:500 dilution). Sections were washed ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were then blocked with 5% nonfat milk at room temperature for 1 hour and then probed with primary antibodies (phospho-p44/42 MAPK, Cell Signaling 9101, 1:4000, dilution) at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... IHC staining with primary antibodies against LC3-I/II (D3U4C) (#12741, 1:2000), Beclin-1 (D40C5) (#3495, 1:400) (Cell Signaling Technology, Danvers, MA, USA) and Ki67 (SP6 ...
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bioRxiv - Cancer Biology 2020Quote: ... The primary antibody and secondary antibodies detailed information list below: PD-L1 [E1L3N®] XP ® Rabbit mAb (#13684, Cell Signaling Technology, Beverly, MA, USA), GAPDH (6C5 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were labeled with the following primary antibodies: SOX2 (goat, R&D, AF2018, 3 μg/mL) and NeuN (rabbit, Cell Signaling Technology, 12943S, 1:500) diluted in antibody blocking solution (1X PBS pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence staining was performed as described previously (Yuan et al., 2020) with primary antibodies to c-Jun (1:1000, Cell Signaling Technology, Danvers, MA, USA) and c-Fos (1:1000 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... every sixth section of the LPB was incubated in a primary antibody solution containing rabbit anti HA-Tag (C29F4) monoclonal antibody (1:1000; 3724s, Cell Signaling Technologies, Danvers, MA, USA) overnight followed by incubation in a solution containing a secondary Alexa-Fluor Plus 488 donkey anti-rabbit antibody (1:3000 ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were blocked with non-fat milk at room temperature for an hour and incubated overnight at 4°C with a primary antibody: anti-P2RX4 (Cell Signaling Technology, Cat# 70659, RRID:AB_2799789) and anti-P2RX7 (Cell Signaling Technology ...
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bioRxiv - Immunology 2022Quote: ... Membrane was blocked using 1x Roti®Block (Carl Roth) for one hour and incubated over night at 4 °C with the primary antibodies against cGAS (Cell Signaling Technology, #31659, 1:1000), β-actin (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was washed again 4 times in TBST for 5 m each and blocked again before incubating with primary antibodies: total Akt (1:1000, Cell Signaling Technology, Cat. #9272S, RRID:AB_329827) & total S6RP (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was incubated for 1 h with 5% bovine serum albumin in 1 x TBS/0.1% Tween (TBST) before being incubated with primary antibodies: pAkt (1:2000, Cell Signaling Technology, Cat. #4060, RRID: AB_2315049), and pS6RP (1:2000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg of protein per lane were subjected to SDS-PAGE and probed with the following primary antibodies: YAP (Cell Signaling Technologies #8418, 1:500), YAP/TAZ (Cell Signaling #4912 ...
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bioRxiv - Microbiology 2020Quote: ... Immunodetection was carried out by using rabbit anti-γ-H2A.X (SPQ) primary antibody (generated using peptide containing the SPQ motif, Cell signaling cat # 9718S, 1:1000), anti-H2A antibody (Abcam cat# ab88770 ...