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Citations for Cell Signaling Technology :
2551 - 2600 of 10000+ citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the slides were blocked with 10% goat serum plus 1% BSA at room temperature for 30 minutes and then incubated with the primary antibody against Ki67 (Cell signaling, Cat #12202S, 1:800) or c-Myc (Cell signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with gentle agitation in primary antibody dilution buffer: phosphorylated ALK (Y1604) (Cell Signaling Technologies, #3341, 1:1000), ALK (Cell Signaling Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.0% Tween-20) for 1 hour at room temperature and probed with primary antibody (1:1000 HA-Tag Rabbit mAb, Cell Signaling, #3724, in 5% milk) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... blocked with 5% nonfat dry milk for 1 hr and probed with primary Abs: phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204, dilution 1:2000, Cell Signaling Technology cat. no. 4370) and alpha tubulin (dilution 1:2000 ...
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bioRxiv - Neuroscience 2019Quote: Immunohistochemical stainings of pERK1/2 and pS6 were performed on 5 μm thick coronal sections of paraffin-embedded mouse brains using primary antibodies raised against pERK1/2 (1:300, Cell Signaling Technology Cat# 4370, RRID:AB_2315112) and pS6 (1:300 ...
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bioRxiv - Biophysics 2020Quote: ... M-phase was discriminated with the phospho-Histone H3 (Ser-10) primary antibody (1:25 in PBS with 1% of NGS, Cell Signaling Technology, USA [RRID:AB 331748]) and the secondary anti-mouse IgG antibody (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue sections of GFP+ tendon cells in each group were observed through a fluorescent microscope and confirmed by immunostaining with rabbit anti-GFP primary antibody (Cat # 2956S, Cell Signaling Technology Inc, Danvers, MA), followed by Cy3-conjugated goat anti-rabbit IgG as the secondary antibody (Cat # AP132C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were incubated overnight at 4°C in a primary antibody solution consisting of rabbit-anti-pSMAD1/5/9 (1:600, Cell Signaling Technology #13820 Lot 3) and goat-anti-SOX2 (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in blocking buffer (10% normal goat serum + 0.3% BSA + 0.3% triton X-100 in PBS) for 1 hour at room temperature and in primary antibodies [anti-p-S6 S240/244 (Cell Signaling Technology #5364, 1:1000) or anti-HCN4 (Alomone Labs APC-052 ...
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bioRxiv - Cancer Biology 2024Quote: ... at room temperature for 1 h before application of primary antibody incubation at 1:500 dilution (Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (Cell Signaling Technology, Cat# 11818, RRID:AB_2687505)) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were extracted at 3 hours post-treatment and analysed by western blot following overnight incubation with antibodies against the following primary proteins: Vinculin (E1E9V, rabbit, Cell Signaling Technology, 13901, 1:1000), Phosphorylated p44/42 MAPK (ERK1/2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked and permeabilized with 5% normal goat serum and 0.3% Triton X-100 in PBS for 1 hour at room temperature and incubated with primary antibodies against STAT1 or STAT3 (Cell Signaling Cats:#14994, #12640 respectively) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... were used as primary antibodies and were detected with anti-rabbit or anti mous IgG HRP-conjugated secondary antibodies (Cell Signaling, ref #7074 and #7076). Proteins were detected by chemiluminescence using Immobilon Forte Western HRP substrate (Merk Rahway ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: Western blotting was performed as previously described (Jin et al., 2021) using primary antibodies against BECN1 (Cell Signaling Technology #3738 and Santa Cruz #sc-48341), FASN (#3180 ...
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bioRxiv - Cancer Biology 2023Quote: ... The intestines were dissected and fixed in 4% paraformaldehyde for 1 h before blocking with 5% NGS and staining with rabbit-PH3 primary antibody (Cell Signaling Technology, Leiden, Netherlands, #3377) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualised using the following primary and secondary antibody combinations: Cyclin B1 was detected with a monoclonal rabbit antibody (Cell Signaling Technology, 12231S, 1:800) and visualised using a goat anti-rabbit Alexa Fluor 647 secondary antibody (Molecular Probes ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (mouse, anti-claudin-4, ThermoFisher, cat: 32-9400, at 1:200 dilution; rabbit, anti-GM130, Cell signaling, cat: 12480, at 1:1600 dilution) were incubated (BSA at 2% ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were treated with the following primary antibodies (αSMA, mouse, Cat. No. ab7817, Abcam at 1:300 dilution, PTEN rabbit, Cat. No. D8H1X, Cell Signaling at 1:300 dilution) sequentially over 2 days with 2 overnight incubations with the primary antibody in blocking buffer (dilution 1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... at room temperature for 1 h and then incubated for 16 h with primary antibodies against the ER tracker protein disulfide isomerase (PDI; rabbit mAb #3501, Cell Signaling Technologies, Danvers, MA, USA) and a loading control GAPDH (rabbit mAb #2118 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 1% BSA for 1 h at 37°C and incubated with primary antibodies: anti-Flag (Cell Signaling, D6W5B, 1:1000 dilution), anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... the coverslips were blocked with 10% goat serum diluted in PBS for 1 hour at RT and then incubated with primary antibodies against Neurofilament-L (Cell Signaling Technology, #2837, 1:100), and β3-tubulin (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed thrice in PBS-T after primary antibody incubation and incubated with secondary antibodies like donkey anti-MouseDyLight 800 (Cell Signaling Technology, cat no#5257) and donkey anti-Rabbit680 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... sections of each brain area were incubated in primary antibody for c-Fos (monoclonal rabbit anti-c-Fos, Cell Signaling, cat mAb-9F6, 1:4000) for 24h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were blocked using 5% non-fat milk in TBS-0.05% tween20 (TBST) and incubated overnight at 4 °C with primary antibodies against P-Rex1 and GAPDH (Cell signaling technology #13168 and #5174 respectively). The membranes were washed three times with TBST and then incubated with secondary antibodies in blocking solution for 2 h at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... s6k1 and tor to determine the cross-link between TOR and RPs using anti-human S6K1 antibody raised in mouse (anti phospho70kDa-S6K1-Thr(P)-389) (Cell Signaling Technologies, cat# 9206). The anti-70S6K1 (CST ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit monoclonal anti-CREB1 1:2,000 diluted in the corresponding blocking solution (5% BSA-TBST) (Cat# 9197, RRID: AB_331277, immunogen: recombinant protein specific to the amino terminus of human CREB1 protein, Cell Signaling Technology, Danvers, MA, USA); rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066 ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Biochemistry 2023Quote: ... at a dilution of 1:100 and 9F3 rabbit monoclonal anti-human β-tubulin from (catalog number 2128, Cell Signaling Technology, Inc., Danvers, MA) at a dilution of 1:5000 ...
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bioRxiv - Microbiology 2019Quote: ... Aβ deposits were detected using primary anti-body specific to isoforms Aβ 37-42 (Aβ D54D2 XP, Cell Signaling Technology, 1:100 dilution, overnight at RT) and further visualized by fluorescent Alexa 568 secondary antibody (1:500 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... Western blots for detecting MAP kinase phosphorylation were probed with primary antibodies against phospho-MAPK1/3 (Thr202/Tyr204) and total MAPK1/3 (Cell Signaling Technology, #4370 and #4696, respectively). MAPK1 is also known as ERK2 or p42MAPK ...
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bioRxiv - Neuroscience 2020Quote: Membranes were then incubated 24-48 hours at 4°C in primary antibodies – PERK (1:500 anti-Rbt; Cell Signaling Technologies [CST], Danvers, MA; cat # 3192S), IRE-1α (1:500 anti-Rbt ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 1 h at room temperature prior to incubation in primary antibody (Novus Biologicals, NBP2-59627 [1:1000 dilution]; Cell Signaling Technology, 2148 [1:1000 dilution]) solubilized in a 1:1 mixture of Odyssey blocking buffer and TBS-T buffer (50 mM Tris Base ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Neuroscience 2020Quote: ... PFA- or NBF-fixed sections were incubated with the following primary antibodies: rabbit anti-cleaved caspase 3-D175 (Cell Signaling, #9661, 1:100, PFA-fixed tissue), Ctip2 (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8 (ab4055), CD4 (EPR6855, ab133616) at a 1:500 dilution and PD-L1 (rabbit monoclonal anit-PD-L1 primary antibody; Cell Signaling Technology, clone: E1L3N, CAT#13684) at a 1:200 dilution on the Leica BOND RX ...
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bioRxiv - Plant Biology 2023Quote: ... Hemagglutinin (HA)-tagged mutant PpRopGAP1 in yeasts was detected with an anti-HA primary antibody (C29F4) of rabbit origin (Cell Signaling Technology, cat. 3724T, 5000× dilution) and an anti-rabbit-HRP (goat ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% Tween-20)) and then incubated with primary antibodies (PC: Proteintech, 16588-1-AP, 1:1000 dilution, or β-actin: Cell Signaling, 8457S, 1:1000 dilution) in TBST supplemented with 5% BSA at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed for 30 min with TBS-0.05% Tween 20 (TBS-T) and primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse secondary antibody diluted 1:2000 (Cell Signaling Technology, Inc., Danvers, MA, USA) and incubated at room temperature for 1 h ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were then washed 3 x 10 minutes in TBS-T and incubated overnight in primary antibodies against phosphorylated Akt T308 (Cell Signaling Technologies #2965; diluted 1:1000), phosphorylated Akt S473 (Cell Signaling Technologies #9271 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated in the following primary antibodies for 3 hours at room temperature: phospho-S6 Ribosomal Protein Ser240/244 (rabbit monoclonal, 1:1000 dilution, Cell Signaling Technology, catalog #5364, RRID: AB_10694233), phospho-AKT Ser473 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/μl (500 μg protein) samples were prepared and incubated overnight at 4 °C in primary antibody against acetyl-lysine (Cell Signaling; Cat No. 9814 (1:100)) ...
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bioRxiv - Biochemistry 2023Quote: ... Bead-bound protein was extracted using the SDS loading buffer and analyzed by SDS-PAGE and western blotting using anti-HA (Primary) antibody (Cell Signaling, Cat#3724S, 1:3000 dilution) and anti-rabbit IgG-HRP (Secondary ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membranes were probed overnight at 4 °C with the specified primary antibodies at the dilution of 1:1000 (Cell Signaling Technology: RIPK1 Rabbit mAb#3493)) or 1:4000 (β-actin Rabbit mAb#4970)) ...
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bioRxiv - Physiology 2020Quote: ... China) at room temperature for 1 h before incubation with a mouse anti-human CD44 monoclonal antibody (Cat. #3570; Cell Signaling Technology, Danvers, MA, USA) and a rabbit anti-human CD24 antibody (Cat ...
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bioRxiv - Plant Biology 2022Quote: SnRK and CDPK in partially purified extracts from peach leaves were evidenced using monoclonal antisera against human phosphorylated-AMPKα (pAMPKα; Cell Signaling Technology, catalog number 2535) and polyclonal antisera against CDPK (Agrisera ...
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bioRxiv - Neuroscience 2023Quote: ... 4 serial brain sections from each animal were stained with rabbit anti-human Aβ antibody (clone D12B2; Cell Signaling Technology Europe, Frankfurt am Main, Germany) and Cy3-conjuagted goat anti-rabbit IgG (Jackson ImmunoResearch Europe Ltd ...
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bioRxiv - Pathology 2021Quote: ... the membranes were incubated in a blocking buffer containing the appropriate diluent of primary antibodies against apoA5 (#3335, Cell Signaling Technology, USA; sc-373950, Santa Cruz, USA), tubulin (66031-1-lg ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated for 48 hr at 4C in 1× PBS containing the primary antibodies (Chicken anti-GFP, AbCam cat. No. #13970. 1:2000; Rabbit anti-NeuN, Cell Signaling Technology, cat. No. #24307, 1:1000). Slices were then washed 3 × 5 minutes in 1x PBS and incubated in 1x PBS containing the secondary antibodies for 2 hours (Alexa Fluor 488 ...