Labshake search
Citations for Cell Signaling Technology :
2351 - 2400 of 10000+ citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... probed with primary antibodies (Supplementary Table 3) and detected with horseradish peroxidase-conjugated α-rabbit or α-mouse secondary antibodies (Cell Signaling). The blots were imaged using a ChemiDoc MP Imaging system (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... in PBST and then incubated for 15 hrs at 4 °C in primary anti-c-Fos (rabbit monoclonal, 1:2000, Cell Signaling 2250) diluted in PBST containing 4% NGS ...
-
bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
-
bioRxiv - Physiology 2024Quote: ... The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA, 1:1000, #5661, Cell Signaling Technology, USA ...
-
bioRxiv - Molecular Biology 2023Quote: ... Membranes were then cut and incubated with different primary antibodies diluted in 5% milk at 4°C overnight: αH3K27me3 (Cell Signaling C36B11) was used at 1:5000 dilution ...
-
bioRxiv - Biophysics 2023Quote: ... The membranes were incubated overnight at 4 °C with primary antibodies against VDAC1 and β-Actin (1:5000, #2146; Cell Signaling Technology) after the washing step with secondary antibodies HRP-conjugate Goat anti-Mouse IgG (H+L ...
-
bioRxiv - Bioengineering 2024Quote: ... The following primary antibodies were used (all rabbit): Vinculin (E1E9V) XP® Rabbit mAb (#13901) and β-actin (#4967S) from Cell Signaling, Danvers ...
-
bioRxiv - Microbiology 2024Quote: ... The membrane was blocked in 5% non- fat milk or bovine serum albumin (BSA) for 1h and incubated overnight at 4°C with primary antibodies against Cyclin T1 (Cell Signaling Technologies), p24 (Abcam) ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight with primary antibody (1:1000 diluted in blocking serum, monoclonal anti-rabbit DCX; Cat#4604S, Cell Signaling Technology) on a shaker (Model# BDRAA115S ...
-
bioRxiv - Cell Biology 2024Quote: ... The required primary antibody (JOSD1 [Abcam, Cambridge, United Kingdom], SOCS1 [Abclonal, Woburn, Massachusetts, United States of America], Cleaved Caspase 3 [Cell Signaling Technology, Danvers ...
-
bioRxiv - Cancer Biology 2024Quote: ... The coverslips were then incubated with primary antibodies against BioID2 (1:5000, BioFront #BID2-CP-100) or HA tag (1:800, Cell Signaling #3724) diluted in 1% BSA/ PBS-Tween 0.1% for one hour in a humidified chamber at RT ...
-
bioRxiv - Developmental Biology 2024Quote: ... The sections were then washed with 1X PBS (2 times, 5 minutes each) and incubated with the primary antibody (COL1A1, Cell signaling #72026S) at 1:50 dilution for 24h at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... After incubation with the primary antibodies (mouse anti-firefly luciferase, clone Luci17; ab16466, Abcam; rabbit monoclonal anti-mouse CD8, 989415, Cell Signaling Technology; rabbit anti-NK1.1/CD161 ...
-
bioRxiv - Neuroscience 2024Quote: ... blocked in TBST plus 5% non-fat milk and then incubated with the following primary antibodies: phospho-S6 (S235/236; 1:2000, Cell Signaling, #4858), S6 (1:2000 ...
-
bioRxiv - Biochemistry 2024Quote: ... which were blocked by 5% non-fat milk (americanBIO, AB10109-01000) and then incubated overnight at 4°C with the following primary antibodies: Nucleocapsid protein (Cell Signaling, #33717), Strep-tagged Nucleocapsid protein (Invitrogen ...
-
bioRxiv - Bioengineering 2024Quote: ... The slides were then labeled overnight at 4℃ with primary antibodies for neurofilament-heavy (RT-97, Developmental Studies Hybridoma Bank, University of Iowa) or cleaved caspase-3 (Cell Signaling 9664S). After three rounds of washing ...
-
bioRxiv - Bioengineering 2024Quote: ... Hydrogels were stained with primary antibodies (mouse anti-KRT7, 1:300, NSJ Bioreagents catalog #V2656; rabbit anti-Ki67, 1:400, Cell Signaling catalog #9129 ...
-
bioRxiv - Biophysics 2024Quote: ... and then were blocked with the 10% normal goat serum for 45 minutes at room temperature before incubating with the primary antibodies (anti-γH2AX: Beyotime, no. C2035S-4; anti-pSTING: Cell Signaling Technology, no ...
-
bioRxiv - Bioengineering 2024Quote: Membranes were subsequently incubated overnight at 4°C with the following primary antibodies: rabbit anti-GAPDH (1:1,000, Cell Signaling Technology #2118) or rabbit anti-HTT ...
-
bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were added overnight in 5% milk in TBS + 0.1% tween-20 (TBST): huntingtin (Clone D7F7, Cell Signaling 5656, 1:1,000), beta-actin (Clone 13E5 ...
-
Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... and incubated with primary antibodies at 1:1000 dilution (overnight at 4°C): rabbit polyclonal anti-phospho (Thr202/Tyr204) Erk1/2 (#9101; Cell Signaling Technology), rabbit polyclonal anti-Erk1/2 (#9102 ...
-
bioRxiv - Molecular Biology 2024Quote: ... and incubated for 16 h with primary antibody (PPARα, Santa Cruz Biotechnology cat# sc-398394; histone H3, Cell Signaling Technologies cat# 14269) diluted 1:1000 in tris-buffered saline (0.5% Tween ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the appropriate primary antibodies were added at 1:50 dilution ratio (Rabbit anti-H3K23ac, Invitorgen; Rabbit anti-H3K27ac, Cell Signaling Technology; CUT&TAG IgG control ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk and incubated with primary antibodies overnight and incubated with anti-rabbit secondary antibody from Cell Signaling Technologies for one hour after which blots were developed using Bio-Rad ChemiDoc™ Imaging Systems ...
-
bioRxiv - Molecular Biology 2024Quote: ... Subsequently membranes were incubated with primary antibodies (1:500 rabbit anti-LEDGF-PWWP [Abcam; ab177159], 1:1000 rabbit anti-MeCP2 [Cell Signaling Technology; 3456S] ...
-
bioRxiv - Cancer Biology 2024Quote: ... Separate sets of slides are stained for imaging in each fluorescent channel with an anti-PSMA rabbit primary antibody (D4S1F, Cell Signaling Technology) and either an IRDye680LT goat anti-rabbit (LI-COR ...
-
bioRxiv - Genetics 2022Quote: ... human anticentromere (1:100; Antibodies Inc., cat# 15-234) and mouse monoclonal anti-H3K9me2/3 (1:100, Cell Signaling, cat# 5327) primary antibodies ...
-
bioRxiv - Cell Biology 2021Quote: Human paraffin embedded pancreas biopsies were sectioned to 3 μm and immunostained with antibodies against MAFB (Cell Signaling Technologies, cat#41019), ARX (R&D Systems ...
-
bioRxiv - Cell Biology 2020Quote: Protein expression levels of PANX1 were examined by western blotting using a rabbit monoclonal antibody against human PANX1 (D9M1C, Cell Signaling). Actin was also detected as a control by western blotting using a mouse monoclonal anti-β-actin antibody (Santa Cruz) ...
-
bioRxiv - Cancer Biology 2022Quote: ... De-identified FFPE tumor specimens were cut into 5 μm sections and IHC performed as described above to stain for human EZH2 (5246, Cell Signaling) and NKp46 (AF1850 ...
-
bioRxiv - Bioengineering 2019Quote: ... cardiac fibroblasts were pre-treated in plastic tissue-culture flasks with 10 nM human TGF-β1 (Cell Signaling Technology, cat# 8915LC) for 48h ...
-
bioRxiv - Cancer Biology 2020Quote: ... Human-specific EpCam-647 (clone VU1D9, #5447) and rabbit anti-human γH2AX antibodies (Phospho-Histone H2A.X Ser139; clone 20E3, #9718) from Cell Signaling Technologies, and EpCam-APC antibody (clone EBA-1 ...
-
Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Human CDH5 and ICAM-1 protein levels were evaluated by SDS PAGE using rabbit anti-CDH5 (Cell signaling technology Cat# 2500) and mouse anti-ICAM-1 (Santa Cruz biotechnology ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with rabbit-anti-human ABCB1 (E1Y7B; mAb #13342; Cell signaling Technology; dilution 1:1000) and mouse-anti-human β-actin (sc-47778 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissues were incubated with primary antibodies (PHOX2B: 1:1000, ab183741, abcam; n-Myc: 1:500, 51705 Cell Signaling Technology; CD45: 1:1000, 70257 Cell Signaling Technology; CD3: 1:2000 A0452 Dako/Agilent) diluted in TBST+5% goat serum in a humid chamber overnight at 4°C ...
-
bioRxiv - Genetics 2021Quote: ... Phosphorylated and total proteins were identified by immunoblotting using the following primary antibodies: Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 and α-Tubulin (DM1A) Mouse mAb #3873 (Cell Signaling Technologies) at 1:1000 dilution ...
-
bioRxiv - Molecular Biology 2021Quote: ... The adductor muscle was additionally incubated with the primary anti-α-smooth muscle actin (α-SMA, 1:500, 19245, Cell Signaling, Danvers, MA). Arteriole density was assessed as α-SMA+ vessels/mm2.
-
bioRxiv - Molecular Biology 2021Quote: ... The following primary and secondary antibodies were used in WB including anti-ICAM1 rabbit antibody (Cell Signaling Technology, Cat. No. 4915S, Danvers, USA), anti-RAB5C rabbit antibody (Thermo ...
-
bioRxiv - Cancer Biology 2019Quote: ... the membranes were incubated overnight at 4 °C with the primary antibodies NF-κB p65 Mouse mAb (Cell Signaling Technology, diluted 1:1000) or TBP Rabbit mAb (Cell Signaling Technology ...
-
bioRxiv - Physiology 2020Quote: ... followed by immersion in 0.3% glycine for 10 min and placed in 0.03% SDS for 10 min then stained with primary IRE1-1α (anti-rabbit, Cell Signaling Tech, 1:100) antibody overnight ...
-
bioRxiv - Neuroscience 2020Quote: ... The membrane was then probed with the following primary antibodies diluted in 5 % BSA /PBS-T for overnight at 4 °C: β-actin (1:1000; Cell Signaling Technology), ZO-1 (1.25 μg/ml) ...
-
bioRxiv - Plant Biology 2021Quote: ... membranes were incubated at room temperature for 1 h with plant monoclonal anti-actin primary antibodies (CusAb, 1:1000) and 1 h with anti-mouse IgG HRP-conjugated secondary antibodies (Cell Signaling, 1:3000). Membranes were washed and developed as described above.
-
bioRxiv - Neuroscience 2020Quote: ... The membranes were probed overnight at 4°C using primary antibodies against p-eIF2α S51 (rabbit, Cell Signaling cat no: 9721, 1:500), anti-t-eIF2α (rabbit ...
-
bioRxiv - Cell Biology 2021Quote: ... Sonicated chromatin was diluted and added to 0.2 ml PCR tube containing primary antibodies (3ug of rabbit anti-NKX6.1 cat. 54551 (Cell Signaling Technology, Danvers, MA, USA); 3 μg of rabbit-anti-acetyl-histone H3-cat ...
-
bioRxiv - Molecular Biology 2022Quote: ... The blocked membrane was incubated overnight at 4°C with primary antibodies diluted in 1x Tris buffered saline with Tween-20 (TBST, Cell Signaling Technology: 9997S). The membrane was washed twice using TBST ...
-
bioRxiv - Systems Biology 2022Quote: ... blocked in 5% dry milk (American bioInc) for 60 min followed by incubation overnight (at 4°C) with primary antibody according to manufacturer’s instruction (Cell Signaling Technology, pSMAD3 (9520S) SMAD3 (9513S) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Immunoblotting analysis was performed with the following monoclonal primary antibodies: rabbit anti p-mTOR (1:1000; #5536, Cell Signaling Technology, Danvers, MA, USA) and rabbit anti mTOR (1:1000 ...
-
bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked followed by overnight incubation at 4°C with primary antibodies: C-terminal Parkin antibody (PRK8; Cell Signaling, 1:40,000 dilution) and N-terminal Parkin antibody (https://mrcppureagents.dundee.ac.uk/reagents-view-antibodies/589302 ...
-
bioRxiv - Neuroscience 2020Quote: ... transferred onto nitrocellulose membranes and detected by immunoblot using the following primary antibodies: mGlu5 (rabbit clone D6E7B from Cell Signaling, dilution 1:1000), actin (mouse clone from DSHB ...
-
bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500; mouse anti-NeuN, Millipore, 1:500; rabbit anti-cleaved caspase-3, Cell Signaling, 1:500) overnight in 3 % BSA in PBS at 4°C ...