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Citations for Cell Signaling Technology :
1851 - 1900 of 1935 citations for Recombinant Human Ribulose 5 Phosphate 3 Epimerase His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% paraformaldehyde for 5 min and subsequently permeabilized with 0.2% Triton-X and stained with anti-relB (Cell Signaling Technology, Danvers, USA) followed by goat-anti-rabbit Alexa 546 (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in 5% skim milk in TBST: horse anti-mouse IgG (1:20,000, Cell Signaling Technology, cat# 7076), donkey anti-sheep IgG (1:20,000 ...
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bioRxiv - Bioengineering 2022Quote: ... The membranes were blocked with PBS with 0.1% Tween-20 (PBST) with 5% Blotto for one hour and then incubated with antibodies to phospho-p90RSK (Thr359/Ser363; #9344; Cell Signaling Technology, Inc.) and RSK1/RSK2/RSK3 (32D7 ...
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bioRxiv - Molecular Biology 2024Quote: ... permeabilized cells were blocked for 30 minutes at room temperature (RT) in 5% (v/v) normal goat serum (NGS) (Cell Signaling Technology, #5425) DPBS solution ...
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bioRxiv - Cancer Biology 2024Quote: ... fixed cells were washed twice in PBS with 0.5% BSA prior to incubation with 1:500 dilution of phospho-histone H3 antibody conjugated with Alexa Fluor 488 (Cell Signaling Techonology, USA) for 1 h in the dark at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... Primary staining was performed on separate blots with the following antibody dilutions in 5% BSA/TBST: rabbit anti-EZH2 1:1000 (Cell Signaling Technology, 5246S), rabbit anti-CBX2 1:500 (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... then probed overnight at 4°C with primary antibodies diluted in a 5% BSA/ 1X TBS-0.1% Tween 20 (TBS-T) solution using either Vinculin (E1E9V) (Cell Signaling, Cat. No. 13901) diluted 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... For live cell microscopy cells were untreated or treated with 5 μM Aβ fibrils (as described above) pre-incubated with an Aβ antibody (Cell Signaling Technologies, #15126) conjugated to an Alexa Fluor 555 secondary (Invitorogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... and blocked in room temperature with 5% BSA (HiMedia, India) solution for an hour and incubated with respective primary antibodies [FOXM1 (1:2000) (Cell Signaling Technologies, USA); active + pro Caspase-3 (1:3000 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked in 5% skimmed milk and incubated overnight at 4°C with primary antibodies for UTX (Cell Signaling Technologies #33510), GFP (JL-8 ...
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bioRxiv - Biochemistry 2023Quote: The antibodies used in this study for immunoblotting (Figure 5) were (1) GST-Tag monoclonal antibody 26H1 (Cell Signaling Technologies catalog number 2624S); (2 ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was blocked for 1 h at room temperature with 5 % BSA dissolved in TBS-Tween (0.1 %) and incubated with the primary antibody rabbit anti-phospho-threonine [(Cell Signaling Technology®, #9381) 1:1000] ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were washed three times for 5 min each with TBS-T then incubated in SignalFire ECL Reagent (Cell Signaling Technology, 6883) for 1 min and imaged using a BioRad ChemiDoc imager.
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitates with anti-lamin A/C antibody were analysed in parallel with 5% unbound supernatants by immunoblotting with anti-lamin A/C (Cell Signaling; Cat# #4777) or anti-His (Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with the primary antibody mAb c-MYC (dilution 1:1000 in TBST/5% milk; Cell Signaling Technology, D84C12). Subsequently ...
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bioRxiv - Physiology 2023Quote: ... was heated at 90°C for 5 minutes and loaded into acrylamide gels before incubated with primary antibodies against catalase (CAT, #14097, Cell Signaling, 1:5000), 3-nitrotyrosine (3-NT ...
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bioRxiv - Cancer Biology 2023Quote: ... Each blot was then washed thrice in 1X TBST for 5 minutes and proteins were detected using either LumiGLO/Peroxide Reagent or SignalFire™ ECL reagent (7003 and 6883, respectively, Cell Signaling Technology) and an iBright™ imaging system (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were blocked with TBST/5% Normal Goat Serum for 1 hour at RT before overnight incubation with primary antibody diluted in Diluent CST (Cell Signaling Technology, 8112) at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... then incubated at 4 °C overnight in TBST with 5% (w/v) BSA and anti-HLA-G (Cell Signaling catalog #79769, 1:1000) and α/β-tubulin (Cell Signaling catalog #2148 ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% BSA in TBS for 1 hr and then incubated overnight at 4° C with anti-pSMAD1/5/8 (1:1000, Cell Signaling Technology, 13820), anti-SMAD1 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking was performed with 5% non-fat milk dissolved in TBST for 1 hour followed by incubation with primary antibodies PRKN (Cell Signaling Technology, 2132) at 1:2000 dilution in 5% BSA in TBST at 4 °C overnight and VCL (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... and blocked with 1x TBST + 5% normal goat serum (NGS) cocktail for 60 min at RT before applying the NFκB primary antibody (Cell Signaling, 6956, 1:1000) in 1x TBST cocktail containing 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... The sections were washed twice with PBS and incubated in 2% BSA plus TBS with primary antibody [1:80 dilution rabbit anti-5-mC antibody (Cell Signaling Technology, #D3S2Z) at 4°C overnight ...
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bioRxiv - Physiology 2024Quote: ... The following primary antibodies were used to detect respective proteins by incubation overnight at 4°C in 5% BSA: CD44 (#E7k2y, Cell signaling, 1:1000), RHAMM (#E7S4Y ...
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bioRxiv - Physiology 2024Quote: ... The PVDF membrane was incubated with 5% w/v non-fat dry milk or 5% BSA for 1 h at room temperature and immunoblotted with anti-HSL (phospho-serine 660) (1:1000, Cell Signaling Technology, 45804S), anti-HSL (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... Pulling down of the pre-cleared chromatin was performed with the use of magnetic beads that were previously incubated with 5 µg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) for 8 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were blocked in 5% bovine serum albumin (BSA) and incubated overnight at 4 °C with primary anti-phospho-SYK (Cell Signaling Technologies, 1:1000) or anti-SYK (Cell Signaling Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were added after blocking for an hour with 5% non-fat milk: STAT3 and Tyr705 pSTAT3 (Cell Signaling Technology, MA, USA), GAPDH (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked in 5% no-fat dry milk in TBST and immunoblotted with rabbit polyclonal anti-CHD7 1:2000 (Cell Signaling Cat. No.6505S), rabbit polyclonal anti-SOX2 1:2000 (Millipore Cat ...
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bioRxiv - Genetics 2019Quote: ... Antibodies used in ChIP are: rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 5 µL per IP); mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Immunology 2021Quote: Cells were fixed with 0.25% paraformaldehyde in ice-cold PBS for 5 min and incubated with anti-ERp57 (#A484, Rabbit mAb, Cell Signaling Technology, Danvers, MA, USA) 1:100 for 40 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl PBS-FC containing 5% FBS with 1:100 diluted PE-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA) and incubated for 30 min in the dark ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed with 1X TBST (3 × 5 min) and then incubated with respective secondary antibodies (Horse anti-mice 1:2000; Horse anti-rabbit 1:2000, (Cell signaling technology, MA, USA) and developed by using ECL chemiluminescent reagent (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 5% BSA in TBS-T before incubation with primary antibodies: phospho-AMPKα Thr172 (1:1000, Cell signaling, MA, USA #2535), β-actin (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... After saturation with TBS Tween 5% BSA for 1h and hybridization of the membranes with primary overnight (BCR-ABL Ab3 Calbiochem, pCrkL Tyr207 Cell Signaling, pTYR 4G10 Millipore) and secondary 1h antibodies coupled to HRP ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was blocked for 2-hours in 1% NGS and 0.3% Triton X-100 followed by 2-3 days of 4C° incubation in 1xPBS (5% NGS, 0.3% Triton X-100) with primary antibody(ies): pS6 S235-236 (Cell Signaling, Catalog: 4858, 1:500), pS6 S244-247(ThermoFisher ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS and incubated with anti-APRT antibody or an anti-β- Actin antibody (Cell Signaling Technology, USA) at 4°C overnight followed by the corresponding secondary antibody for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2019Quote: ... Blots were probed overnight at 4 deg C with rabbit anti-V5 (Cell Signaling Technologies; 1:1000 in 5% BSA, 0.1% Tween 20, TBS) or for 1 hr at room temperature with mouse anti-β actin (GeneTex ...
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FGF signalling is involved in cumulus migration in the common house spider Parasteatoda tepidariorumbioRxiv - Developmental Biology 2021Quote: ... Fluorescent pMad antibody staining was performed using the Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Cell Signaling Technology, Inc.; antibody concentration: 1:1000) as primary and an Alexa488 anti rabbit AB (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked in 5% milk and incubated overnight at 4 °C in primary antibody (anti-PPARγ, Santa Cruz #sc-7273; or anti-β-actin, Cell Signaling #3700). Washes were performed in TBS-T (20 mM Tris ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed three times for 5 min per time with shaking then incubated with secondary antibodies (anti-mouse IgG, HRP-linked antibody, Cell Signaling, Danvers, MA, USA) diluted in 5% w/v nonfat milk at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated overnight at 4°C with antibodies for the designated antigens INTEGRIN α5 (1:1000, #4705), INTEGRIN β3 (1:1000, #4702), β-ACTIN(1:1000, #8457), β-TUBULIN (1:1000, #2146) purchased from Cell Signaling Technology, GAPDH (1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution, or rabbit anti-pMET, #3077, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Physiology 2023Quote: ... blots were washed with TBS-T three times and then incubated in TBS-T containing 5% non-fat dry milk and anti-biotin horseradish peroxidase (Cell Signaling, Danvers, MA, USA) and HRP-conjugated donkey anti-chicken antibody (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated with the following primary antibodies for 2 hours at room temperature in 5% BSA: 1:1000 anti-SMARCC1 (Cell Signaling Technologies cat. #11956T), 1:1000 anti-SMARCA4 (Cell Signaling Technologies cat ...
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bioRxiv - Genetics 2023Quote: ... 0.1% Tween 20 and 5% BSA overnight at 4 °C with anti-Runx2 antibody (RUNX2 D1H7 Rabbit mAb, Cell Signaling Technology, 84866 1:2000) and anti-β-actin antibody (Rabbit Anti-beta Actin ...