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Citations for Cell Signaling Technology :
1701 - 1750 of 1935 citations for Recombinant Human Ribulose 5 Phosphate 3 Epimerase His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 10 minutes in 0.2% Triton X-100 and blocked for 1 hour in 5% NDS and 0.05% Triton X-100 in PBS before incubation with rabbit anti Smad3 (1:250, #9624, Cell Signaling), mouse anti PCBP1 (1:200 ...
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bioRxiv - Biochemistry 2021Quote: ... incubation with a horseradish peroxidase-coupled anti-mouse lgG (Cell Signaling Technology; 1:1000 in TBST with 5% (w/v) non-fat dry milk) ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 μm cryosections were incubated overnight at 4°C with an antiphosphoSmad1/5/8 antibody (1:500; Cell Signaling 9511S) in PBS with 2% horse serum and 0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μg of antibody (anti-PUM1, Santa Cruz sc-135049; anti-PUM2, Santa Cruz sc-31535; anti-RBMX Cell Signaling #14794 ...
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bioRxiv - Molecular Biology 2019Quote: ... BMP signaling was measured using a monoclonal phospho-SMAD 1/5/9(8) antibody (Cell Signaling Technology, Danvers, MA, #13820) at a dilution of 1:1000 ...
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bioRxiv - Plant Biology 2019Quote: ... Detection of phosphorylated proteins was performed in blocking solution (5% BSA, 0.1% [v/v] Tween-20) with anti-phosphothreonine antibodies (9381, Cell Signaling) and with anti-rabbit-HRP (W4011 ...
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to Phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling Technology, 9211S, 1:2500 in 5% BSA for WB); Rabbit polyclonal antibodies to iNOS (Santa Cruz Biotechnology ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated in 5% non-fat dry milk in 1× PBS and 0.1% Tween 20 with the primary antibodies (PHC1 Cell Signaling #13505 1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% milk in PBS-T (0.1% Tween 20) and incubated with rabbit anti-mitofusin 2 (mAb D2D10, Cell Signaling), rabbit anti-mitofusin 1 (mAb 13196S ...
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bioRxiv - Cell Biology 2022Quote: ... washed three times with PBS and blocked for 1h in PBS containing 5% normal goat serum (Cell signaling, Cat # #5425) and 0.3% Triton X-100 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in 5% milk before probing with the following antibodies: rabbit anti-SHIP1 (1:1000, Cell Signaling D1163) and rabbit anti-β actin (1:2500 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were then blocked with 5% non-fat dry milk in Tris-buffered saline (#9997, Cell Signaling Technology, USA) with 0.1% Tween 20 (P1379 ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunoprecipitations were carried out as previously described63 on approximately 5 ug of chromatin (minimum 2.5 ug, maximum 8.8 ug) using 5 ul of total RNA Pol II antibody (Cell Signaling D8L4Y). Libraries were prepared using the NEBNext Ultra II DNA Library Kit for Illumina (NEB ...
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bioRxiv - Biophysics 2023Quote: ... blots were incubated in blocking buffer (5%(w/v) milk powder (nonfat dry milk, Cell Signaling Technology, Danvers, MA, USA)) in TBST containing 25 mM Tris base ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C in 5% BSA/TTBS containing rabbit anti-STING (0.058 µg/ml, Cat. # 13647, RRID: AB_2732796; Cell Signaling Technology), anti-phospho-TMEM173/STING S366 (1 µg/ml ...
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bioRxiv - Physiology 2023Quote: ... blocked with 5% goat serum and incubated overnight at 4 °C with anti-TMEM16A (Cell Signaling, Rabbit D1M9Q 1:100) and CD31 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% BSA TBST for 1h at 24 prior to incubating with STAT3 (Cat #12640, RRID:AB_2629499, Cell Signaling Technologies) or NF- kB (p65 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nitrocellulose membrane was blotted with primary antibodies in TBS containing 0.05% Tween and 5% BSA (Cell signaling; p65-RelA (8242), phospho-p65-RelA-Ser536 (3033) ...
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bioRxiv - Cell Biology 2023Quote: ... and staining was performed after blocking with 5% normal goat serum with anti-Insulin (Linco) and anti-Glucagon (Cell Signaling) antibodies using established protocols ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked in 5% milk and incubated with primary antibody: anti-Phospho-IRF3 (Ser 396) (D601M, Cell Signaling), anti-IRF3 (D6I4C ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% milk in 1X TTBS and incubated overnight with the appropriate primary antibody (Cell Signaling c-IAP2 58C7 Rabbit mAb #3130 ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were washed 3X 5 min with TBS-T and incubated with secondary antibody at 1:500 (#7074, Cell Signaling) in 1% BSA in TBS-T for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated in primary antibody 1:20 mouse anti-AP2 (Developmental Studies Hybridoma Bank, University of Iowa, 3B5 supernatant) and 1:300 rabbit anti-Cleaved Caspase 3 (Cell Signaling Technology, Danvers, MA #9661) o/n in humid chamber ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were labeled with the following primary antibodies: SOX2 (goat, R&D, AF2018, 3 μg/mL) and NeuN (rabbit, Cell Signaling Technology, 12943S, 1:500) diluted in antibody blocking solution (1X PBS pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664, 1:4000 anti-α-Tubulin Sigma #T5168, 1:1000 anti-PARP Cell Signaling Technologies #9542 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were dissolved with 3% BSA in tris buffered saline (TBST) and membranes were incubated overnight at 4°C with SQSTM1/p62 (Cell Signaling, cat# 88588, 1:1000). The primary LC3 A/B (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... Representative 20X maximum intensity projections of apoptotic cells in the ganglion cell layer labeled by primary antibody against cleaved (active) caspase-3 (Cell Signaling Technology Cat# 9661, RRID:AB_2341188).
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bioRxiv - Neuroscience 2023Quote: ... blocked with 5% milk in TBS/0.1% Tween-20 (TBST) and incubated with anti-phospho-eNOS (Thr495) and anti-eNOS (Suppl Table 3; 1:1,000, Cell Signaling cat. #9574 and 9572, respectively). Membranes were washed in TBST ...
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bioRxiv - Developmental Biology 2023Quote: We used an antibody for phospho histone 3 (pH3) that was previously reported(Shirae-Kurabayashi et al, 2006) (pH3-ser10-6g3-mouse-mAb, Cell Signaling; #9706; 1:500 diluted). The primary antibody was added together with anti-DIG antibody during FISH process ...
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bioRxiv - Microbiology 2024Quote: ... and cell extracts were incubated for 3 h at 4°C with beads pre-coupled overnight with rabbit anti-LC3C (Cell Signaling Technology, 14736, 1:250) antibody ...
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bioRxiv - Cancer Biology 2024Quote: Quantification of hepatocyte and tumor cell apoptosis was performed in liver slides by anti-cleaved caspase-3 immunostaining (Cell Signaling Technology #9661, diluted 1:400), detected with HRP-bound goat anti-rabbit secondary antibody (Invitrogen #31460 ...
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bioRxiv - Neuroscience 2020Quote: ... PFA- or NBF-fixed sections were incubated with the following primary antibodies: rabbit anti-cleaved caspase 3-D175 (Cell Signaling, #9661, 1:100, PFA-fixed tissue), Ctip2 (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were stained with primary antibodies at 4°C overnight at the following dilutions: rabbit anti-Vg (a gift from Sean Carroll and Kristen Guss, 1:200) and rabbit anti-Caspase 3 (Cell Signaling Technology, Cat# 9661, 1:200), mouse anti-beta Galactosidase (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated with TBS and 3% horse serum and 0.25% Triton X-100 before being incubated with Alexa Fluor© 488 Anti-Rabbit IgG (Cell Signaling, cat#4412S, diluted 1:250) for 2 hours at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were then washed 3 x 10 minutes in TBS-T and incubated overnight in primary antibodies against phosphorylated Akt T308 (Cell Signaling Technologies #2965; diluted 1:1000), phosphorylated Akt S473 (Cell Signaling Technologies #9271 ...
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bioRxiv - Developmental Biology 2023Quote: ... portions of the entire pancreas were sectioned at 5μm thickness and ≥3 non-consecutive sections of each portion were stained with an antibody against insulin (1:100, Cell Signaling Technologies: 4590, Danvers, MA, USA), which was subsequently detected by biotinylated goat anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following primary antibodies were diluted 1:1000 in 3% BSA/T-TBS and incubated for about 16 h: anti LC3-I/II (cat. # 12741S) from Cell Signaling Technologies (distributed by Euro-Clone); anti-α-Tubulin (cat ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated in the following primary antibodies for 3 hours at room temperature: phospho-S6 Ribosomal Protein Ser240/244 (rabbit monoclonal, 1:1000 dilution, Cell Signaling Technology, catalog #5364, RRID: AB_10694233), phospho-AKT Ser473 (rabbit monoclonal ...
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with methanol/acetone (50/50) and then stained intracellularly by incubating cells with rabbit antibody against p-(Y694) STAT-5 (D47E7) XP (Cell Signaling), mouse antibody against BCL-2 (100 ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were stained with polyclonal anti-TCF7L1 antibody raised in rabbit (1:100 for complete slides, 1:25 for TMAs; 28835 clone D15G11 lot 5, Cell Signaling). For TMA analysis ...
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bioRxiv - Cell Biology 2019Quote: ... 100,000 cells were plated in each well the same day of the assay and allowed to attach for 5 hours in growth media in the presence of 5μM ROCK inhibitor (13624, Cell Signaling Technology). Subsequently growth media was replaced with Seahorse base media (102353-100 ...
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bioRxiv - Genetics 2021Quote: ... containing 5% (w/v) skimmed dried milk powder and immunoblotted using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody (#2851 Cell Signaling) (dilution 1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated overnight at 4°C with primary antibodies diluted in 5% goat serum/ PBS: rabbit anti-PAX6 (1:250, 60433S, Cell Signaling), rabbit anti-SOX1 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies and dilutions (in 5% milk in TBST) were used for western blotting: E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), N-cadherin (#13116 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked for 1 h at room temperature with 5% skim milk and then incubated overnight at 4°C with appropriate primary antibody dilution: anti-STAT1 (1:1000, Cell Signaling), anti-pSTAT1 (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... and probed with the following antibodies diluted in 5% skim milk/PBS (w/v): anti-HA (1:4000, clone C29F4, Cell Signaling), anti-cMyc (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with either 5% skimmed milk or 5% bovine-serum albumin (BSA) and incubated with dilution of respective antibodies as per manufacturer’s instructions (Cell Signaling technologies). The membrane was washed ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Developmental Biology 2022Quote: ... antigens were retrieved by boiling slides in citrate buffer (pH 6.0) for 1 min and pSMAD1/5 Ab (Cell Signaling, 41D10) was added overnight at 4°C ...