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Citations for Cell Signaling Technology :
1651 - 1700 of 1935 citations for Recombinant Human Ribulose 5 Phosphate 3 Epimerase His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-otoferlin (Developmental Studies Hybridoma Bank, HCS-1) diluted at 1:100 and rabbit anti-cleaved caspase-3 (Cell Signaling Technology, 9661) diluted at 1:250 ...
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bioRxiv - Genetics 2024Quote: ... and then fixed at different time points (1 hour, 3 hours, 6 hours and 24 hours) before staining for DNA damage markers: ψH2AX (Cell Signaling Technology # 9718T), 53BP1 ((Novus Biologicals # NB100-904 ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked with 3% BSA in PBS for 1 hour and cells were stained with VEGFR2 (Cell Signaling; C#2479; 1:200) and EEA1 (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were identified in paraffin-embedded sections by immuno-staining with activated cleaved caspase 3 antibody (Cell Signaling Technologies, Inc., Danvers, MA). Immunohistochemistry was performed using guinea pig antiinsulin primary antibody (Fitzgerald Labs ...
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bioRxiv - Cancer Biology 2023Quote: ... OV90 and CAOV3 cells were cultured in a glass bottom 96 well plate as described and stained with Cleaved caspase 3 (CC3) antibody (Cell signaling technology: 9661T) at 1:100 dilution after 12 hours incubation with T cells for CAOV3 and 48 hours incubation for OV90 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and Western blot performed on resultant protein as described by us.[85] Samples were probed using antibody against cleaved caspase 3 (Cell Signaling Technology, #9664) and β-actin (Santa Cruz ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μg of digested chromatin samples was treated with antibodies against rabbit IgG (1:100; Cell Signaling Technology, 2729) or NKX2.1 (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked with 5% milk powder in TBST for 1 h and probed with SETD2 (Cell signaling, 80290) 1:1000 overnight at 4°C with agitation ...
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bioRxiv - Systems Biology 2020Quote: ... then incubated with anti-vimentin antibody (clone D21H3, Cell Signaling Technologies, (1:500 dilution in 5% milk/TBS-T) and with anti-GAPDH antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked with 5% powdered milk in buffer and probed with pan-SynGAP (1:1,000, #5539, Cell Signaling) or SynGAP-α2 (abcam ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then incubated with 1× TBST containing 5% milk and either anti-RPS6KB1 (Cell Signaling Technology, 2940S) or anti-DNAJC3 (Cell Signaling Technology ...
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bioRxiv - Pathology 2022Quote: ... Membranes were stained with Ponceau-S and blocked for 30 minutes with 5% nonfat dry milk (Cell signaling, 9999) or 5% BSA in Blotto buffer (0.15M NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... The following primary antibodies were used: Phospho-Smad1/5 (Ser463/465) (41D10) (1:300, Rabbit, Cell Signaling Technology #9516S), P-Histone H3 (1:500 ...
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) (Cell Signaling Technology, 4668S, 1:2500 in 5% BSA); Rabbit polyclonal antibodies to p38 MAPK (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: EMT was induced in the MCF10A cell line by chronic stimulation with 5 ng/ml TGFβ1 (Cell Signaling Technology) for eight days22 ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with anti-NTMT1 (1:1000 dilution in 5% BSA in TBST buffer, Cell signaling #13432) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated at 4°C for 5 h with antibodies for BRD4 (Cell Signaling Technology; 1:100). Antibody/antigen complexes were recovered using Dynabeads Protein A/G (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked with 5% BSA and incubated with rabbit mTOR (1:1000, Cell Signaling Technologies, USA), rabbit p-mTOR (1:1000 ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% milk and probed using either 1:1000 antiV5 antibody (Cell Signaling Technology, 13202) or 1:10000 GAPDH as a loading control (Proteintech 60004-1) ...
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bioRxiv - Biophysics 2023Quote: ... Each blot was blocked with 10 mL 5% (w/v) nonfat dry milk (Cell Signaling Technology, Danvers, MA, USA) in TBST buffer (25 mM Tris base ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with 5% skimmed milk in Tris-Buffered Saline with Tween 20 (TBST; Cell Signaling Technology) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were first incubated with blocking and permeabilization buffer (1X PBS / 5% normal goat serum (Cell Signaling, cat #5425) / 0.3% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was blotted overnight with primary antibodies in TBS-T containing 5% BSA (Cell signaling antibodies; Stat1 (9172), phospho-Stat1-Tyr701-(7649) ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked with 5% nonfat dried milk and then incubated with primary antibodies from Cell Signaling Technology that recognize ...
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bioRxiv - Cell Biology 2023Quote: Cullin-RING E3 ubiquitin ligases were inhibited by addition of 5 μM MLN4924 (Cell Signaling Technology, catalog no.: 85923) and the 26S proteasome was blocked with 10 μM MG132 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) analysis was performed on a QuantStudio™ 5 Real-Time PCR System (Cell Signaling Technology, MA) using the SYBR green reaction mix (Vazyme Biotech ...
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bioRxiv - Bioengineering 2023Quote: ... anti-pSmad 1/5/9 (ref: 13820S) and anti-pSmad 2 (ref: 18338S) antibodies were obtained from Cell signaling. The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% BSA TBST and incubated in the primary antbodies anti-INSR (Cell Signaling Technology, #3025), anti-TdTomato (SICGEN ...
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bioRxiv - Microbiology 2020Quote: ... for evaluation of histopathologic changes by H&E staining or for immunohistochemical detection of reovirus antigen (1:45,000 dilution) or the cleaved (active) form of caspase-3 (Cell Signaling, 9664; 1:300 dilution). Images were captured at a magnification of ×20 and a resolution of 0.5 μm/pixel using a high-throughput Leica SCN400 slide scanner automated digital imaging system.
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bioRxiv - Molecular Biology 2020Quote: ... phospho-eIF2α (Ser51) clone D9G8 #3398) or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169 ...
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bioRxiv - Cell Biology 2020Quote: ... A polyclonal antibody against phosphorylated Tyr416 of Src and a polyclonal antibody against mouse caspase-3 were from Cell Signaling Technology (Beverly, MA, USA). α-Thrombin was purchased from Enzyme Research Laboratories (South Bend ...
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bioRxiv - Genomics 2020Quote: ... sections were blocked in 3% bovine serum albumin (BSA) and incubated over-night with antibodies recognizing Birc5 (#2808, Cell Signaling Technology, Danvers, MA, USA), Cyclin D1 (#2978 ...
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bioRxiv - Neuroscience 2019Quote: ... Total and phosphorylated glycogen synthase kinase 3 (GSK3-β) were detected using rabbit antibodies anti-GSK3-β (9315) and anti-pGSK3α/β-Ser21/9 (9331) (Cell Signaling). Chemiluminescence signals were analyzed using Image Lab software 6.0 (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... PFA-fixed sections were incubated with the following primary antibodies overnight at 4°C: rabbit anti-cleaved caspase 3 (Cell signaling, #9661, 1:100), mouse anti-BrdU (Dako ...
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bioRxiv - Neuroscience 2021Quote: ... gift from Carmen Birchmeier and Thomas Müller of the Max Delbrück Center for Molecular Medicine in Berlin) and rabbit anti-cleaved Caspase-3 (1:1000, Cell Signaling Technology Cat# 9661, RRID:AB_2341188). SCs were then washed in PBS and incubated for 2 h at RT with secondary fluorescent antibodies (goat anti-rabbit-conjugated 649 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis, France) and rabbit polyclonal anti-cleaved caspase 3 antibody was provided by Cell Signaling Technology (Ozyme, Saint Cyr l’ecole, France). All Alexa-conjugated secondary antibodies were purchased from Thermo Fisher Scientific.
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Cancer Biology 2022Quote: ... acetyl-histone 3 (Lys27, #8173, D5E4), histone3 (#4499, D1H2), EP300 (# 86377, D8Z4E), and CREBBP (# 7389, D6C5) were purchased from Cell Signaling Technologies (Danvers, MA). An anti-acetyl-SMC3 antibody was a kind gift from Forma Therapeutics ...
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bioRxiv - Cell Biology 2023Quote: ... The expression of arrestin-3 and GPCRs was determined by western blot using anti-arrestin F431 [32] anti-HA (#3724, Cell signaling Technology, Danvers, MA) antibodies.
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bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were detected by TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining using in situ apoptosis detection kit (Takata Bio Inc., Shiga, Japan) or immunostaining of cleaved caspase-3 (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with PBS and then were blocked with 5% (wt/vol) BSA and 0.1% Triton X-100 in PBS before staining with mouse anti-gH2AX (Cell Signaling), rabbit anti-RPA pS4/S8 (Bethyl) ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by incubation with primary Antibody against pSMAD1/5/8 (the antibody was purchased from Cell Signaling Technology; no. 9511) at 1:200 dilution in blocking solution ...
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bioRxiv - Molecular Biology 2020Quote: ... BMP signaling was measured using a monoclonal phospho-SMAD 1/5/9(8) antibody (Cell Signaling Technology, Danvers, MA, #13820) at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked for 1 h using 5% BSA in TBS-T (0.01% Triton-X) and incubated overnight with primary antibodies (1:500 pSTAT1, 7649 Cell Signaling; 1:500 pSTAT3 ...
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bioRxiv - Neuroscience 2019Quote: ... diluted with TBS containing 0,1% Tween 20 (TBS-T) and 5% non-fat dry milk: rabbit HRP-conjugated actin 1:10000 (Cell Signaling); rabbit p-JNK and JNK 1:200 (Santa Cruz) ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated overnight at 4C with primary antibody diluted in TBS (Fisher) supplemented with 0.1% Tween20 (Fisher) and either 5% BSA (Cell Signaling) or 5% non-fat dry milk (Oxoid) ...
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bioRxiv - Microbiology 2021Quote: ... Blots were blocked in 0.05% tween-20/5% Non-fat milk in Tris-buffered saline and probed with the indicated antibodies from Cell Signaling: GAPDH (#3683) ...