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Citations for Cell Signaling Technology :
1751 - 1800 of 1935 citations for Recombinant Human Ribulose 5 Phosphate 3 Epimerase His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... fresh frozen OCT-embedded embryo lung sections (5 μm thick) were stained with anti-E-Cadherin (Cell Signaling, Cat No 3195), anti-Snail (Santa Cruz ...
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bioRxiv - Genetics 2019Quote: ... the membrane was blocked with 5% skimmed milk in PBST and subsequently probed with anti-LC3 (Cell Signaling, 12741, 1/2000), washed and incubated with HRP-conjugated secondary antibody (1:10 000 ...
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bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were then incubated o/n at 4C with 5% milk powder in PBS-T containing the primary antibodies at the following dilutions (1:1000 Lin28a (A177, Cell signaling); 1:4000 H3 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T, #9102, Cell Signaling) and phospho-p44/42 MAPK (Thr202/Tyr204 ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies (in 5% milk in TBST) were used for western blotting: anti-E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), anti-N-cadherin (#BDB610920 ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C and secondary HRP-conjugated anti-rabbit IgG (1:2,000 in 5% nonfat dry milk; Cell Signaling Technology) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... Cells were treated with 5% goat serum for 30 min and incubated with antibodies against TRPC3 (Alomone Labs) or NFkB-p65 (Cell Signaling) for 1h ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was blocked for 1h with 5% BSA in TBS-T followed by overnight incubation at 4°C with antibody (Drosha 1:1000; D28B1, Cell Signaling, in 5 %BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... The chromatin was then incubated with 5 µg of antibody against KDM6A (A302-374A, Bethyl Laboratories and 33510S, Cell Signaling Technology), KDM6B (ab169197 ...
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bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... followed by blocking in 5% BSA with 0.2% Triton X-100 for 30min and incubation with primary antibody Axin1 (#2087, 1:200, Cell Signaling Technology) overnight ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology) secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were blocked with 3% BSA in PBS overnight at 4°C and then incubated with a rabbit phospho-SMAD1/5/9 antibody at 1:400 (D5B10, Cell Signaling) and a mouse anti-YAP antibody at 1:200 (63.7 ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with PBS-0.1% Tween 20 (PBS-T) containing 5% milk and were incubated with rabbit monoclonal anti-CYLD (1:500, #8462 Cell Signaling) and mouse monoclonal anti-β-actin (1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... then permeabilized with 0.1% Saponin for 5 minutes and probed with anti-H3K4me3 (Cell Signaling 12064S, 1:150 in 0.1% Saponin), anti-H3K9ac (Cell Signaling C5B11 ...
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bioRxiv - Plant Biology 2023Quote: ... Blots for detecting phosphorylated MAPK were blocked in 5% BSA and immunoblotted with a phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit antibody produced in goat (Agrisera ...
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bioRxiv - Cancer Biology 2023Quote: ... The extract was then incubated with 5 μg of Anti-FLAG Antibody (DYKDDDDK Tag (D6W5B) Rabbit mAb#14793 antibody (Cell Signaling) and 50 μL of protein G beads at 4 ºC overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed 3x 5 mins with PBS and then stained with corresponding secondary antibodies (1:1000; 4414S Cell Signaling Technology, 8889S Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803 ...
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bioRxiv - Neuroscience 2023Quote: ... All antibodies were diluted in 1X PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membrane was shaken three times for 5 min in TBST then incubated with 1:3,000 anti-rabbit HRP-linked antibody (Cell Signaling Technologies) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked for 1h and probed with 5% nonfat dry milk with the following primary antibodies: anti-IRF8 (clone D20D8, Cat# 5628, Cell Signaling), anti-CD74 (sheep anti-mouse cd74 ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies were diluted 1:1000 in 5% milk/TBST (FGFR1: D8E4; GAPDH HRP-conjugated: 14C10; Cell Signaling, Danvers, MA, USA). The membranes were incubated with antibodies at 4°C overnight ...
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bioRxiv - Physiology 2024Quote: ... C2C12’s were replated and allowed 24 hours to recover after which the labeled C2C12 myoblasts were induced for apoptosis using 5 µM Staurosporin (cat. no.: 9953S, Cell Signaling) for 18h ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then blocked and permeabilized for 1 hour at room temperature while rocking with 1mL of blocking buffer (0.3% Triton X 100 and 5% normal goat serum (Cell Signaling Technology) in 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... cells were permeabilized with 100% cold methanol and stained with anti-phosphorylated (p)SMAD1/5/9 (Cell Signaling Technology, Danvers MA) overnight at a 1:50 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... were blocked in TBST buffer (Tris-buffered saline and 0.15% Tween-20) containing 5% nonfat dry milk (#9999, Cell Signaling Technology). The blots were probed overnight with primary antibodies diluted in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Revert stain was then removed and then membranes were blocked in 5% BSA for 2 hours at RT and then incubated overnight at 4°C in either anti-NLRP3 antibody (15101S, 1:1000 in 5% BSA, Cell Signaling), anti-NLRC4 antibody (12421 ...
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bioRxiv - Immunology 2024Quote: ... Membranes were blocked for 1 h in 5% non-fat milk in 1X TBST at room temperature and incubated with primary antibodies against KEAP1 (#8047, Cell Signaling), NQO1 (#11451-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were processed for Nissl staining (Cresyl violet staining) or immunohistochemistry with antibodies against cleaved caspase-3 (Asp175; Cell Signaling Technology, Inc. Danvers, MA, USA; 1:500) and c-Fos (PC05 ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858), S6 (Cell Signaling 2217) ...
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bioRxiv - Cell Biology 2020Quote: ... Transferred blots were blocked for 1 hour in 5% w/v non-fat dry milk in 1X TBST (Cell Signaling Cat# 9997). Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858) ...
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bioRxiv - Cell Biology 2020Quote: For immunoblotting the following primary antibodies were used at a concentration of 1:1000 in 5 % milk-TBS-T: HA (Cell Signaling 3724S), HA (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk and incubated in 2% BSA with primary antibodies to GAPDH (Cell Signaling Technology Cat# 13084, RRID:AB_2713924), Eg5 (Sigma Aldrich Cat# SAB4501650 ...
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bioRxiv - Cell Biology 2020Quote: ... the pellet was washed with PBS (–) and incubated on ice for 5 min in 0.1 ml cell lysis buffer (Cell Signaling Technology, Inc.). In the other conditions ...
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bioRxiv - Genetics 2021Quote: ... The following primary antibodies were used at 1:1000 dilution in PBST (0.05% Tween-20) and 5% non-fat dry milk and incubated overnight at 4°C: Myc (Cell Signaling Technology #2276), RBPJ (Cell Signaling Technology #5313) ...
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bioRxiv - Molecular Biology 2021Quote: ... washed three times with 1x PBS and incubated with 5 % horse serum/1xPBS for 1 h at 20 °C before incubating with the following antibodies (Cell Signaling Laboratories): anti-calnexin (clone C5C9) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibody incubations were done in 5% milk/PBS using rabbit anti-HA at 1:500 (Cell Signaling, Cat #: 3724S) and anti-Rabbit HRP at 1:10,000 (BioRad ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were washed four times with TBST (5 minutes gentle rocking per wash) and incubated overnight with primary antibody Rabbit anti-Gli-1 (Cell signaling, #C68H3); 1:250 dilution in TBST-Milk at 4°C with gentle rocking ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was then washed with Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and blocked in 5% nonfat dry milk (Cell Signaling Technology, #9999) diluted with TBS-T for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... After 3 washing steps (5 min each) with PBS-T membranes were incubated with horseradish-linked goat anti-rat (1:2000 dilution; 7077S; Cell Signaling Technology) or goat anti-rabbit (1:2000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074; Cell Signaling, Switzerland) and goat anti-mouse antibody (7076 ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316, 1:1000 dilution in 5% milk/TBST; rabbit anti-β-actin loading control Cell Signaling #8457 ...
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bioRxiv - Neuroscience 2020Quote: ... 5 days after irradiation the plates were assayed for senescence via the Senescence β-galactosidase Cell Staining Kit (Cell Signaling, Catalog # 9860) and the staining was performed as per manufacturer’s protocol.
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bioRxiv - Biochemistry 2021Quote: ... Slices were blocked with 5% donkey serum (37°C, 30 min) and then incubated with a primary antibody targeting RIPK3 (#95702, Cell Signaling Technology) at 4°C overnight diluted in antibody diluent (Dako) ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked with 5% non-fat milk/PBS for 30 min and incubated with primary antibodies as follows: anti-PDGFRA (Cell Signaling, 3174T), anti-COL1A1 (Proteintech ...