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Citations for Cell Signaling Technology :
1551 - 1600 of 1935 citations for Recombinant Human Ribulose 5 Phosphate 3 Epimerase His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 3% BSA overnight at 4 □C (rabbit α-LC3B 1:1000 – Cell Signaling/ #4108, rabbit α-Atg5 1:1000 – Cell Signaling/#2630 ...
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bioRxiv - Neuroscience 2023Quote: ... The fixed and permeabilized cells were then labelled with antibodies against cleaved caspase 3 (Cell Signaling Technology; 9761; 1:500 dilution) and counterstained with DAPI ...
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bioRxiv - Biophysics 2019Quote: ... chemically fixed cells were permeabilized in 0.1% Triton-X 100 then labeled with anti-human phosphotyrosine CD79A (Tyr182) rabbit antibody (Cell Signaling Technology Cat# 5173, RRID:AB_10694763). The primary antibody was detected using one of two a secondary goat anti rabbit polyclonal antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... s6k1 and tor to determine the cross-link between TOR and RPs using anti-human S6K1 antibody raised in mouse (anti phospho70kDa-S6K1-Thr(P)-389) (Cell Signaling Technologies, cat# 9206). The anti-70S6K1 (CST ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Biochemistry 2023Quote: ... at a dilution of 1:100 and 9F3 rabbit monoclonal anti-human β-tubulin from (catalog number 2128, Cell Signaling Technology, Inc., Danvers, MA) at a dilution of 1:5000 ...
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bioRxiv - Cell Biology 2019Quote: ... Non-fat milk (5%) was used as a blocking solution and anti–mouse IgG (7076P2; Cell Signaling) and ECL Select (RPN2235 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-P-SP from rabbit (#9111, Cell Signaling, at 1:2000 dilution for WB in 5%BSA), anti-P-TP from rabbit (#14371 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-P-TP from rabbit (#14371, Cell Signaling, at 1:2000 dilution for WB in 5% BSA), anti-Cdk1 PSTAIR from mouse (#10345 ...
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bioRxiv - Cell Biology 2020Quote: Antibodies and conditions were: anti-pSer235/236-RPS6 (1:1000, 5% BSA, #4858, Cell Signaling, MA, USA), anti-RPS6 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.2% TritonX-100 and 5% Normal Goat Serum) of rabbit anti-c-Fos (Cell Signaling, #2250,1:700) for two days at 4°C ...
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bioRxiv - Genetics 2019Quote: ... fixed with 4% paraformaldehyde for 30 minutes and then blocked with 5% normal goat serum (Cell Signaling) and 0.3% Triton X-100 for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked with 5% milk before incubation with primary antibodies calnexin (1/1000, Cell Signaling Technology), BIP (1/40000 ...
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bioRxiv - Bioengineering 2021Quote: ... blocked in 5% milk and subjected to Western blot analysis using antibodies for Notch2 (Cell Signaling, D76A6) and GAPDH (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with PBS then permeabilized and blocked with 5% normal goat serum (Cell Signaling Technology) and 1% BSA in PBS containing 0.3% Triton X-100 (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... Spheroids were then washed with DPBS and blocked with 5% Normal Goat Serum (NGS) (Cell Signaling Technology) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... The fixed cells were incubated for 1 h in blocking buffer [5% Normal Goat Serum (Cell Signaling) in PBS with 0.3% TritonX100 (Sigma)] ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST + 5% NFM and HRP-linked goat anti-rabbit IgG (1:1000, Cell Signaling Technology, #7074) in TBST + 5% NFM ...
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bioRxiv - Immunology 2020Quote: Cell lysates from 5-10 × 106 cells were generated using CHAPS Cell Extract Buffer (Cell Signaling Technology) supplemented with 1X Halt protease inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to p38 MAPK (Cell Signaling Technology, 9212S, 1:2500 in 5% BSA for WB); Rabbit polyclonal antibodies to Phospho-p38 MAPK (Thr180/Tyr182 ...
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to SAPK/JNK (Cell Signaling Technology, 9252S, 1:2500 in 5% BSA for WB); Rabbit polyclonal antibodies to Phospho-SAPK/JNK (Thr183/Tyr185 ...
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bioRxiv - Microbiology 2020Quote: ... and phospho-p44/42 MAPK (Thr202/Tyr204) (1:1000 in 5% BSA/TBS-T, #9106, Cell Signaling). Actin was detected using anti-actin antibody (1:2000 in 5% BSA/TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were diluted in 5% BSA/TBS as follows: rabbit anti-Cx43 (Cat. No. 3512; Cell Signaling) at 1:2000 and mouse anti-Vinculin (Cat ...
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bioRxiv - Immunology 2022Quote: ... sections were blocked for 1 hour in TBS/T containing 5% normal goat serum (Cell Signaling Technology) and then incubated with primary antibodies listed in Table S1 overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were blocked for 1 h with 5 % non-fat dry milk (Cell Signaling Technology, 9999) in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.4) supplemented with 5 % BSA and HRP-conjugated rabbit anti-mouse antibody (Cell Signaling Technologies, USA) diluted 1:2000 as a secondary antibody.
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% milk following an incubation with anti-HA antibody (Cell Signaling Technology, #3724) to detect EPHB2 ubiquitination levels ...
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bioRxiv - Immunology 2023Quote: ... blocked with 5% BSA and incubated with monoclonal rabbit anti-HIF-1α (Cell Signaling Technology, clone D1S7W). Detection was carried out with a goat anti-rabbit horse radish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2021Quote: ... Single clones were picked out after 3 weeks and Cas9 expression level in each clone was determined by western blotting with Cas9 antibody (Cell Signaling, #14697). Cas9 activity was tested using the CRISPRtest™ Functional Cas9 Activity Kit for Human Cells (Cellecta ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained for 30 minutes at room temperature with Alexa Fluor 647-conjugated anti-cleaved Caspase-3 (9602, Cell Signaling Technology) and analysed by flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved caspase-3 was probed in MuSCs by incubating the cells overnight in 1:100 Cleaved Caspase-3 (Asp175) (5A1E) rabbit mAb (Cell Signaling Technology). MuSCs were stained for 3 hours with 1:500 donkey anti-rabbit Alexa 594 secondary antibody (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos/explants were then blocked in PBS+ 10% FBS and 1% Triton X-100 and incubated overnight at 4°C with an anti-pSmad2/3 antibody (Cell Signaling #8828) at 1:1000 in block ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated at 4°C overnight with the following primary antibodies diluted in buffer G: rabbit polyclonal anti-cleaved caspase-3 (Asp175) (Cell Signaling, #9661) at 1:250 ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Cell Biology 2021Quote: ... and Cleaved Caspase 3 (#9664, 1:400 dilution for IF) and anti-Rsk2 (#5528; 1:200 dilution for IF) were purchased from Cell Signaling Technology. Anti-Tubulin α (66031-1-Ig ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... Flare Biotech LLC) following by HRP conjugated goat anti-rabbit antibody (1:10,000 Ab in 3 % BSA in TBST; Cell Signaling Technology Inc). Images obtained were analyzed via ImageJ software.
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bioRxiv - Cancer Biology 2022Quote: ... and ɣH2AX (NB100-384) were from Novus; cleaved Caspase-3 (5A1E; 9664) and pS10 Histone H3 (9701) were from Cell Signaling Technology; GAPDH (6C5 ...
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bioRxiv - Physiology 2022Quote: ... antibody in 0.1% TBST containing 3% BSA overnight at +4°C followed by incubation with secondary HRP-linked anti-rabbit antibody (Cell Signaling, #7074). Immunoblotted proteins were detected with ProSignal Pico ECL Reagent (Genesee Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... sections were blocked with 3% bovine serum albumin / TBS and then incubated with primary antibodies (rabbit anti α-actinin (Cell Signaling, 3134)) overnight at 4 °C at 1:30 ...
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bioRxiv - Bioengineering 2021Quote: ... The fixed samples were permeabilized in PBS containing 0.1% Triton X-100 and 1% Fetal Bovine Serum for 30 min at room temperature before filling the channels with staining buffer (1.5% BSA in PBS) containing primary antibodies directed against cleaved caspase-3 (Cell Signaling Technology, 9661S) or VE-cadherin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Galectin-3 (#87985, IF: 1:400) and Na/K-ATPase α1 (#23565, IF: 1:100) antibodies were purchased from Cell Signaling Technology. Anti-beta-actin antibody (A2228 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then stained overnight at 4°C with AlexaFluor 647-conjugated anti-β-catenin antibody L54E2 at 1:300 dilution supplemented with 3% BSA (2677S, Cell Signaling). Between and after each of these steps ...
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bioRxiv - Bioengineering 2021Quote: ... brains were formalin fixed and paraffin embedded before staining with cleaved caspase-3 (CC3 Rabbit Mab, 1:800 #9664L [D175] Cell Signaling Technology) and rabbit polymer secondary (Biocare Medical # RMR 622L) ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies (diluted in 3% w/v BSA in TBST) were used: adipose triglyceride lipase (ATGL) (1:1000, Cell Signaling #2138), perilipin5 (PLIN5 ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed 3 times with TBST and then incubated with peroxidase-conjugated secondary antibody (Cell Signaling Technology, Cat.No. 7074S, 1:3000). The antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Cell Biology 2022Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...