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Citations for Cell Signaling Technology :
8951 - 9000 of 9030 citations for GAPDH Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Membranes were probed for the vascular content of COX-1 (anti-COX-1, cat#: 4841S, source: rabbit, 1:2000; Cell Signaling Technology, Danvers, MA, USA) and COX-2 (anti-COX-2 ...
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bioRxiv - Cell Biology 2021Quote: ... 2% normal goat serum and 0.2% Triton-X100 in PBS) for 30 minutes and hybridized with polyclonal rabbit anti-phospho-Histone H3 (Ser10) antibody (Cell Signaling Technology, #9701; 1:1000) at overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue sections of GFP+ tendon cells in each group were observed through a fluorescent microscope and confirmed by immunostaining with rabbit anti-GFP primary antibody (Cat # 2956S, Cell Signaling Technology Inc, Danvers, MA), followed by Cy3-conjugated goat anti-rabbit IgG as the secondary antibody (Cat # AP132C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were incubated overnight at 4°C in a primary antibody solution consisting of rabbit-anti-pSMAD1/5/9 (1:600, Cell Signaling Technology #13820 Lot 3) and goat-anti-SOX2 (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... stored as free-floating sections in phosphate buffered saline at 4°C and then incubated with a rabbit antibody against cFos (1:1000, Cell signaling #2250, Danvers, MA, USA) for 48 h ...
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bioRxiv - Pathology 2022Quote: ... 4 µm sections of formalin-fixed paraffin-embedded tissue were mounted on positively charged Superfrost® Plus slides and subjected to IHC using an anti-nucleocapsid rabbit monoclonal antibody (HL344, Cell Signaling Technology, Danvers, Massachusetts). IHC was performed using the automated BOND-RXm platform and the Polymer Refine Red Detection kit (Leica Biosystems ...
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bioRxiv - Systems Biology 2022Quote: ... Immunoblot analysis was used to assess the adequacy of nuclear purification by measuring Lamin A/C (rabbit, 1:1000, Cell signaling # 2032, a nuclear protein).
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed twice with TBST and incubated for 1 hour with HRP-linked anti-rabbit IgG or anti-mouse IgG (Cell Signaling, 1:5000 – 1:10,000) at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... phospho-HER2 (pY1221/1222) and pHER4 (1284) levels were determined by Western Blot using following antibodies: rabbit anti-HER4 (Cell Signaling, Cat# 111B2, 1:1000), rabbit anti-phospho-Y1283 HER4 (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... The HRP-conjugated secondary antibodies (anti-rabbit and anti-mouse antibodies, respectively ref 7074 and 7076) were supplied by Cell Signaling Technologies (Danvers, Massachusetts, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were extracted at 3 hours post-treatment and analysed by western blot following overnight incubation with antibodies against the following primary proteins: Vinculin (E1E9V, rabbit, Cell Signaling Technology, 13901, 1:1000), Phosphorylated p44/42 MAPK (ERK1/2 ...
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bioRxiv - Neuroscience 2024Quote: ... after which the sections were incubated overnight at 4°C with rabbit anti-cFos antibody (1:6400 dilution, #2250, Cell Signaling Technology, Danvers, MA, USA), 1% NHS ...
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bioRxiv - Cancer Biology 2023Quote: ... The HRP-conjugated secondary antibodies (anti-rabbit and anti-mouse antibodies, respectively ref 7074 and 7076) were supplied by Cell Signaling Technologies (Danvers, Massachussets, USA).
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bioRxiv - Cancer Biology 2023Quote: ... were used as primary antibodies and were detected with anti-rabbit or anti mous IgG HRP-conjugated secondary antibodies (Cell Signaling, ref #7074 and #7076). Proteins were detected by chemiluminescence using Immobilon Forte Western HRP substrate (Merk Rahway ...
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bioRxiv - Cancer Biology 2023Quote: ... and secondary horseradish peroxidase (HRP)-conjugated antibodies goat anti-rabbit IgG (#7074) and horse anti-mouse IgG (#7076) were purchased from Cell Signaling Technology (Danvers, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Oligodendrocyte differentiation was assessed by incubating free-floating sections overnight at room temperature with rabbit PDGFRα (D1E1E) XP® antibodies (Cell Signaling, cat #3174,1:200), mouse anti-CC1 (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed three times in TBST for 5 minutes and incubated for 1 hour with horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology Cat# 7074, RRID:AB_2099233) or anti-mouse IgG (Cell Signaling Technology Cat# 7076 ...
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bioRxiv - Plant Biology 2023Quote: ... MAPK activation was detected by western blot with Phospho-p44/42 MAPK (Erk1/2; Thr-202/Tyr-204) rabbit monoclonal antibodies #9101 (Cell Signaling Technology, Danvers, MA, USA). Blots were stained with PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... used at 1:500 (western blot) and 1:50 (immunofluorescence) and rabbit anti–phospho-MLC2 raised against phospho-Thr18/Ser19 (Cell Signaling Technology; catalog no. 3674) used at 1:500 (western blot ...
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bioRxiv - Cancer Biology 2023Quote: ... The intestines were dissected and fixed in 4% paraformaldehyde for 1 h before blocking with 5% NGS and staining with rabbit-PH3 primary antibody (Cell Signaling Technology, Leiden, Netherlands, #3377) overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... The membrane was then washed three times in the wash buffer for five minutes each and incubated for one hour at room temperature with agitation in an anti-rabbit HRP-conjugated secondary antibody (Cell Signaling Technology, 7074V, 1:1000) diluted in the wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualised using the following primary and secondary antibody combinations: Cyclin B1 was detected with a monoclonal rabbit antibody (Cell Signaling Technology, 12231S, 1:800) and visualised using a goat anti-rabbit Alexa Fluor 647 secondary antibody (Molecular Probes ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (mouse, anti-claudin-4, ThermoFisher, cat: 32-9400, at 1:200 dilution; rabbit, anti-GM130, Cell signaling, cat: 12480, at 1:1600 dilution) were incubated (BSA at 2% ...
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bioRxiv - Cancer Biology 2024Quote: ... sonicated chromatin was supplemented to final concentration of 1.1% Triton-X and 5% glycerol and subsequently set up for immunoprecipitation with the following antibodies: Rabbit Anti-POU2F3 (Cell Signaling E5N2D, #36135, 1:50), mouse anti-Rpb1 (Cell Signaling 4H8 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed three times with TBST for 5 minutes and immunoblotted with the appropriate HRP-conjugated secondary antibodies: anti-rabbit (Cell Signaling, Cat#7074, 1:2000) or anti-mouse (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: Antibodies against pAKT (Ser473) pAKT (Thr308) and AKT (pan) (all rabbit polyclonal) and antibody against β-actin was from Cell Signaling Technology (Danvers, MA, USA). Antibody against fibronectin (ab6328 ...
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bioRxiv - Physiology 2023Quote: ... 5% low-fat dry milk (blocking solution) and incubated overnight at 4°C with a rabbit monoclonal anti-Numb antibody (#2756, Cell Signaling Technology, Inc., Danvers, MA) at 1:1000 dilution in 5% BSA in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... 4 serial brain sections from each animal were stained with rabbit anti-human Aβ antibody (clone D12B2; Cell Signaling Technology Europe, Frankfurt am Main, Germany) and Cy3-conjuagted goat anti-rabbit IgG (Jackson ImmunoResearch Europe Ltd ...
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bioRxiv - Biophysics 2023Quote: ... Detection was performed with the UVP ChemStudio Imaging System (Analytik Jena, Jena, Germany) using horseradish peroxidase-linked anti-mouse (#38) and anti-rabbit (#29) secondary antibodies (Cell Signaling Technology, Danvers, MA, USA), and the ECL western blotting reagent ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were treated with the following primary antibodies (αSMA, mouse, Cat. No. ab7817, Abcam at 1:300 dilution, PTEN rabbit, Cat. No. D8H1X, Cell Signaling at 1:300 dilution) sequentially over 2 days with 2 overnight incubations with the primary antibody in blocking buffer (dilution 1:200 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Membranes were washed 3 x and incubated for 1 h at room temperature with the horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse-HRP, 1:2000; Cell Signaling Technology, Danvers, MA, USA), washed in LSWB and incubated with chemiluminescent HRP substrate reagent (Super Signal West Femto ...
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bioRxiv - Microbiology 2024Quote: ... and cell extracts were incubated for 3 h at 4°C with beads pre-coupled overnight with rabbit anti-LC3C (Cell Signaling Technology, 14736, 1:250) antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated with secondary antibodies (goat anti-rabbit monoclonal IgG, HRP-linked, 1:1000, Cell Signaling Technology, #7074S; horse anti-mouse IgG monoclonal, HRP-linked, 1:2000, Cell Signaling Technology, #7076S) diluted in blocking solution for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-GM130 (BD transduction Laboratories, ref:610822, 1:1000, rabbit ITGB1, ITGB4, ITGA4 (from integrin Ab Sampler kit, Cell signaling ref: 4749, 1:1000), rabbit anti-EGFR (Cell Signaling ref ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were washed with TBST for 10 min three times before incubation with secondary antibodies [Anti-rabbit IgG-HRP (Cell Signaling Technology 7074S, 1:5000), Anti-mouse IgG- HRP (Cell Signaling Technology 7076S ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was washed with TBST and incubated in TBST + 5% (w/v) nonfat milk and anti-rabbit IgG HRP-linked antibody (Cell Signaling catalog #7074, 1:1000) for 1 hour then washed in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 5% goat serum/2.5% BSA for at least 1 hour before overnight incubation at 4°C with an antibody cocktail consisting of rabbit anti-vimentin (1:100) (catalog #: 5741; Cell Signaling Technologies; Danvers, MA, USA), mouse anti-dystrophin IgG2b (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit monoclonal antibodies against TRIM46 (#1 in text: Abcam Cat# ab307967, RRID:AB_3096363, 1:500, #2 in text: Cell Signaling Technology Cat# 92574, RRID:AB_3096364, 1:1000), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308 ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 4-times for 10 min in 1 x PBS-T before 1 h incubation at room temperature with secondary anti-rabbit IgG coupled to horseradish peroxidase (Cell Signaling Technology, Danvers,MA, USA) diluted 1:1,000 in 1 x PBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... slices were moved to the primary antibody solution containing one or more of the following antibodies in blocking solution and were left there overnight: rabbit-anti-c-Fos (1:1000; Cell Signaling Technology, 2250s, Leiden, Netherlands), chicken-anti-GFP(1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with a FACS buffer (FACS solution supplemented with 4% fetal calf serum) and incubated with anti-rabbit secondary antibody Alexa Fluor 488™ (#4412, Cell Signaling Technology, Danvers, MA, USA)(1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... we used identical procedures except that we used LV-Syn-DIO-mTagBFP2-2A-GluN2B (1 × 109 TU/ml titer, 2 μL) and GluN2B antibody (#4207S, Cell Signaling, 1:1000, rabbit polyclonal antibody) (Supplementary Fig ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed and incubated with HRP-conjugated secondary antibodies at 1:10,000 (Mouse, Cell Signaling technology Cat# 7076, RRID AB_330924; Rabbit, Cell Signaling technology Cat# 7074, RRID AB_2099233) for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Neuroscience 2020Quote: ... PFA- or NBF-fixed sections were incubated with the following primary antibodies: rabbit anti-cleaved caspase 3-D175 (Cell Signaling, #9661, 1:100, PFA-fixed tissue), Ctip2 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated with TBS and 3% horse serum and 0.25% Triton X-100 before being incubated with Alexa Fluor© 488 Anti-Rabbit IgG (Cell Signaling, cat#4412S, diluted 1:250) for 2 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... at 1:100 in 3% Casein in 1X Tris Buffered Saline with TWEEN ® 20 (Sigma-Aldrich, Oakville, Ontario, and Anti-rabbit HRP-linked Antibody (7074S - Cell Signaling Technology, Danvers, MA) at 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated at room temperature with HRP-conjugated secondary antibodies ((goat anti-rabbit HRP conjugated, Cell Signaling, Cat #: 7074, 1:5,000 dilution) (goat anti-mouse HRP conjugated, Cell Signaling, Cat #: 7076, 1:5,000 dilution) prepared in the WB blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... NF-KB (1:1000 mouse anti-total NF-κB; Cell Signaling Technology #6956; RRID #AB_10828935 or 1:1000 rabbit anti-phospho NF-κB-S536; Cell Signaling Technology #3033; RRID #AB_331284); Akt (1:250 mouse anti-total Akt ...