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Citations for Cell Signaling Technology :
8901 - 8950 of 9030 citations for GAPDH Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated with horseradish peroxidase-conjugated secondary antibodies (anti-rabbit IgG at 1:5000 dilution or anti-mouse IgG at 1:5000 dilution; both from Cell Signaling Technology, Danvers, MA), and the bound proteins were visualized using a chemiluminescence-based detection kit (ImmunoStar Zeta ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The membrane was washed four times in 0.1% TBST buffer and incubated for 1 h at room temperature with anti-rabbit IgG conjugated to HRP-linked antibody #7074 (Cell Signaling Technology, Danvers, MA) diluted 1:5,000 with 0.05% TBST containing 5% skimmed milk ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 0.5% BSA–PBS and incubated with a horseradish peroxidase-conjugated goat anti-rabbit antibody (30 ng/ml) (Cell Signaling Technology, Danvers, MA, USA) in 1% BSA–PBS for 1 h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... antibodies and the secondary anti-rabbit horseradish peroxidase-linked IgG antibody (NA944V, GE Healthcare for western blotting and 7074S Cell signaling Technology for IHC) were purchased from the indicated vendors ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:4700 in 5% non-fat milk (anti-rabbit IgG #ADI-SAB-300-J, Enzo Lifesciences, Farmingdale NY or anti-mouse IgG #7076, Cell Signaling Technology, Danvers MA). The membranes were imaged using Azure Biosystems Radiance Plus substrate solution and an Azure c600 Imaging System.
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bioRxiv - Biochemistry 2023Quote: ... Coverslips were then blocked with 5% BSA for 60 minutes at room temperature and incubated overnight at 4°C with primary antibody γH2AX (rabbit monoclonal; Cell Signaling 9718T, 1:400). Texas red species-specific secondary antibodies (Jackson ImmunoResearch Laboratories Inc. ...
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bioRxiv - Genomics 2023Quote: ... The blots were washed with 1X TBST (6 washes of 5 min each) followed by incubation with HRP-conjugated anti-rabbit secondary antibody (Cat. No. 7074S, Cell Signaling Technology, MA, USA) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... CUT&RUN was performed from 500,000 cells per condition in triplicates as previously described in (Skene et al, 2018) using the following antibodies: rabbit anti-HA (Cell Signaling Technology, Cat# 3724); rabbit anti-MLL1 (Epicypher ...
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bioRxiv - Neuroscience 2023Quote: ... for 1h at RT and then immunolabeled with rabbit primary antibodies, anti-GIRK2 (1:500, Alomone labs, APC-006, RRID: AB_2040115) and anti-actin (1:2000, Cell Signaling Technology, 4970S, RRID: AB_2223172) overnight at 4°C in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit monoclonal antibody against β-actin (clone 13E5) or rabbit polyclonal antibody against α-tubulin (Cat.-No.: 2144) (both antibodies were from Cell Signaling Technology) was used as a protein loading control ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, catalog # 7074). Then the membranes were washed with TBST four times ...
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-Phospho-IκBα (Ser32/36) (5A5) (catalogue number: 9246) and rabbit anti-Phospho-IKKα/β (Ser176/180) (16A6) (catalogue number: 2697) were from Cell Signaling Technology (CST). Monoclonal mouse anti-IKKβ (10AG2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% BSA diluted in TBS-Tween0,1% for 1 hour and primary antibodies were incubated overnight (rabbit anti-HER2 #29D8 Cell Signaling; mouse anti-vinculin #A250291). Donkey anti-mouse IgG or goat anti-rabbit IgG secondary antibody conjugated to horse-radish peroxidase (Jackson ImmunoResearch Laboratories ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Cells were incubated overnight at 4° C with rabbit anti-pSMAD1/5/9 (1:600 dilution, Cat: 13820, Cell Signaling Technology, Danvers, MA USA). The secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies were used as follows: goat anti-rabbit IgG HRP linked Antibody (Cell Signaling Technology, 7074S, 1:2000), or goat anti-mouse IgG HRP (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... The MEL cells were bound to Concanavalin A Beads and Incubated with 1:50 rabbit polyclonal Phospho-Rpb1 CTD Ser5 and Ser2 antibody (Cell Signaling 13523 and 13499). Guinea pig a-rabbit antibody was used at 1:100 dilution as secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... at a dilution of 1:100 and 9F3 rabbit monoclonal anti-human β-tubulin from (catalog number 2128, Cell Signaling Technology, Inc., Danvers, MA) at a dilution of 1:5000 ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed with PBS-T and they were incubated with goat anti-rabbit IgG H&L Alexa Fluor 488 (Cell Signaling Technology, MA, USA) for 90 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and incubated overnight at 4°C with mild agitation in 5% milk in TBST plus indicated antibody: cytochrome c (1:750, rabbit, Cell Signaling Technology, Cat. 11940), GAPDH (1:10000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The oocytes were incubated with primary antibodies (Alexa Fluor® 488 Conjugate anti-α-tubulin monoclonal antibody,1:200, Cell Signaling, Cat#35652; rabbit anti-Tom20 antibody, 1:100, Cell Signaling, Cat#sc-42406 ...
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bioRxiv - Cancer Biology 2023Quote: ... The FGFR1-probed membranes were washed 5x for 5min in TBST and then probed with anti-rabbit HRP-conjugated antibody (7074S, Cell Signaling, Danvers, MA, USA) for 1h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated for 48 hours at 4°C in PBS-T-containing rabbit anti-IGF1R β antibody (1:1000; Cell signaling, Cat#9750), which has been previously validated [46] ...
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bioRxiv - Microbiology 2024Quote: ... These cells were stained with 50 µM DAPI and incubated at 4 °C overnight in PBS-BSA containing rabbit anti-RNF2 IgG (clone D22F2, Cell Signaling Technology, Denver, MA) and mouse anti-NS5A IgG (clone 9E10 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The tissues were then incubated overnight at 4°C with anti-DeltaFosB primary antibody (DeltaFOSB; rabbit; Cell Signaling Technology, ref. 22509; 1:1000 dilution) or anti-Fos antibody (c-Fos ...
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bioRxiv - Cell Biology 2024Quote: ... we incubated the cells with fluorescent secondary FAB fragments raised to the species corresponding to the primary antibody for 1 hr in PBS supplemented with 10% FBS (either Anti-rabbit IgG Fab2 Alexa Fluor ® 555 or Anti-mouse IgG Fab2 Alexa Flour ® 488, both Cell Signaling Technology, Catalogue # 647 4414 S and # 4408 S ...
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bioRxiv - Microbiology 2024Quote: ... The samples were washed twice with PBS-T and incubated with an AF488-conjugated anti-rabbit antibody (Cell Signaling Technology, Inc., Danvers, MA, USA) at a 1:1,000 dilution in PBS-T at room temperature for 30 min ...
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bioRxiv - Cell Biology 2019Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) rabbit antibody (no. 3674, Cell Signaling Technology, 1:50 dilution); anti-E-cadherin rabbit antibody (no ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were rinsed three times for 5 min in TBS 0.1% Tween-20 and blots were probed with either HRP-conjugated anti-rabbit or anti-mouse secondary antibody (Cell Signaling, cat: 7074 and 7076, respectively). Membranes were then rinsed three times and treated with the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Whole embryos were fixed in 4% paraformaldehyde overnight and stained with anti-phospho-Smad1/5/9 Rabbit monoclonal antibody (Cell Signaling Technology #13820S; 1:500), anti-phospho-H3 Rabbit monoclonal antibody (Cell Signaling Technology #9701S ...
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bioRxiv - Biochemistry 2019Quote: ... The following secondary antibodies were used: horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody and HRP-conjugated anti-rabbit IgG antibody (1:3000 dilution, Cell Signaling Technology, Beverly, Massachusetts, USA). HRP-dependent luminescence was developed with Western Lightning Chemiluminescent Reagent Plus (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated in primary antibody 1:20 mouse anti-AP2 (Developmental Studies Hybridoma Bank, University of Iowa, 3B5 supernatant) and 1:300 rabbit anti-Cleaved Caspase 3 (Cell Signaling Technology, Danvers, MA #9661) o/n in humid chamber ...
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bioRxiv - Biochemistry 2020Quote: ... Free-floating sections were blocked with 5% donkey serum for 1.5 hours before overnight incubation at 4°C with primary antibodies (goat anti-Albumin antibody, Abcam ab19194, 1:100 dilution; rabbit anti-V5 antibody, Cell Signaling Technology, 1:500 dilution). Sections were washed ...
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bioRxiv - Neuroscience 2020Quote: ... For detection of AAV-mediated spCas9 expression the same immunohistochemistry protocol was followed but with the addition of an antibody against HA (Cell Signaling, Rabbit C29F4, 1:500).
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bioRxiv - Neuroscience 2020Quote: ... incubated in HRP-conjugated secondary antibodies (donkey anti mouse HRP, Jackson ImmunoResearch, Cat. #715-035-150 or Goat anti rabbit HRP: Cell Signaling Technology, Cat. # 7074S). Membranes probed for p62 were washed with 0.01% TBS-Triton X and incubated in biotinylated anti-guinea pig (goat polyclonal ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed 3 times for 10 minutes in TBS-T buffer and then incubated in secondary antibodies made against mouse and rabbit as appropriate in milk at a dilution of 1:4000 (Cell Signaling Technologies, Danvers, MA, USA). Membranes were washed again 3 times for 20 minutes and then developed using the BioRad imaging system (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then washed three times for 5 minutes each with 1X TBST and incubated with a secondary anti-rabbit horseradish peroxidase-conjugated antibody (Cell Signaling, Cat # 7074S, 1:2000) in 5% BSA for two hours at room temperature with gentle rocking ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...
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bioRxiv - Neuroscience 2020Quote: ... The IP product was then run on a Western blot and probed using both the rabbit anti-mouse panAkt (1:1000) and Phosopho-Akt (Ser473, Cell Signaling Technologies 4060; 1:1000) antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were labeled with the following primary antibodies: SOX2 (goat, R&D, AF2018, 3 μg/mL) and NeuN (rabbit, Cell Signaling Technology, 12943S, 1:500) diluted in antibody blocking solution (1X PBS pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated in secondary antibody (HRP conjugated anti-rabbit or anti-mouse IgG(H+L) (1:10,000 dilution, Cell Signaling, cat # 7074P2 or 32230, respectively). Subsequently the signal was developed using Super Signal West Femto substrate (ThermoFisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... every sixth section of the LPB was incubated in a primary antibody solution containing rabbit anti HA-Tag (C29F4) monoclonal antibody (1:1000; 3724s, Cell Signaling Technologies, Danvers, MA, USA) overnight followed by incubation in a solution containing a secondary Alexa-Fluor Plus 488 donkey anti-rabbit antibody (1:3000 ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was washed four times with PBS-T and incubated with DyLight 800 goat anti-rabbit IgG (Cell Signaling Technology, cat. no. 5151, RRID:AB_10697505) diluted 1:10,000 in PBS-T containing 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Immunodetection was carried out by using rabbit anti-γ-H2A.X (SPQ) primary antibody (generated using peptide containing the SPQ motif, Cell signaling cat # 9718S, 1:1000), anti-H2A antibody (Abcam cat# ab88770 ...
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bioRxiv - Bioengineering 2019Quote: ... 1:50, Sigma-Aldrich, cat. nr. HPA050007; goat αV5 antibody, 1:350, Abcam cat. nr. ab95038; and, rabbit αHA, 1:800, Cell Signaling cat. nr. C29F4) at 4°C overnight ...
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bioRxiv - Bioengineering 2021Quote: ... The membrane was washed three times with X1 TBST for 10 min each and incubated with HRP□conjugated rabbit or mouse secondary antibodies (#7074 and #7076, Cell Signaling Technology Inc., Danvers, MA) at 1:5000 dilution in X1 TBST with 5% nonfat dry milk for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Phosphorylation of MAPKs Slt2 and Fus3 was detected using a rabbit antiPhospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204; #4370 from Cell Signaling Technology, Danvers, MA, USA). A mouse anti-α-tubulin antibody (#T9026 from Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... Cells on coverslips were incubated with diluted primary antibodies in PBST against myosin light chain-2 (MLC-2, Cell Signaling Technology, 3672S, Rabbit, 1:100), RAR-β (Abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were transferred to a PVDF membrane and blocked for one hour in 5% dry milk before incubation with KIF3A (D7G3) rabbit monoclonal antibody (Cell Signaling Technology, Danvers, MA, #8507) in TBS containing 0.1% Tween-20 and 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were detected from the blots using the following primary antibodies diluted in the appropriate ratio with 1x TBST and incubated overnight at +4°C: rabbit anti-phospho-Akt1 (S473, 1:1000, #9018, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-Akt2 (S474 ...