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Citations for Cell Signaling Technology :
8651 - 8700 of 9030 citations for GAPDH Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 m at RT and incubated in primary antibody rabbit anti–Calreticulin at 1:100 (Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Pathology 2023Quote: ... formalin fixed paraffin embedded tissue sections were stained with rabbit-anti claudin-2 (1:100, Cell Signaling Technology Cat# 28530, RRID: AB_2798961), and rat-anti CD45 (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 488-conjugated rabbit monoclonal anti-phospho-histone H3 (pH3) (1:200 for IF and 1:50 for flow cytometry; D2C8; Cell Signaling Technology); and fluorescence (Alexa Fluor 488 or Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were detected secondaries antibodies diluted in PBS with 1% goat serum: polyclonal Alexafluor 594-conjugated anti-rabbit IgG (1:1000, Cell Signaling, 8889), and polyclonal Alexafluor 488-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution using the following concentrations: 1:1000 anti β-actin (13E5) rabbit primary antibody (#4970 from Cell Signaling), 1:1000 anti-tdtomato rabbit primary antibody (#632496 from Takara) ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution (1:1000 anti β-actin (13E5) rabbit primary antibody #4970 from Cell Signaling) (1:1000 anti GAPDH ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed 3X with TBST for a total of 15 minutes and incubated with HRP conjugated secondary anti-mouse (7076S) or anti-rabbit (7074S) antibodies at a 1:5000 ratio (Cell Signaling Technology) for 1 hour at room temperature with gentle rocking ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary and secondary antibodies were diluted in FACS buffer and used 1:100 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058) and 1:500 for anti-rabbit Alexa-647 (goat ...
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bioRxiv - Cell Biology 2024Quote: ... Specimens were incubated overnight at 4 °C with rabbit anti-SARS-CoV-2 nucleocapsid protein (Cell Signaling Technology, USA, #26369, 1:200), mouse anti-ACE2 (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP, 7076; Cell Signaling) for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The membranes were probed overnight at 4°C with rabbit monoclonal antibodies against inducible nitric oxide synthase (iNOS; Cell Signaling Technology, 2982) or arginase-1 (Arg-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 4×106 cells were fixed in methanol and incubated with anti-pS6 (Ser235/236) rabbit antibody (1:100, Cell Signaling Technology #2211), followed by incubation with cell surface markers and secondary Alexa Fluor 647 goat anti-rabbit (1:200 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were then washed 3 times with PBST and were incubated with HRP-conjugated anti-rabbit IgG (1:500 dilution, catalog 7074S, Cell Signaling Technology) for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated overnight at 4°C with various antibodies: rabbit anti-pan ubiquitin (1:1,000, #3933S; Cell Signaling Technology, Danvers, MA), rabbit anti-K48-linkage specific polyUb (D9D5 ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... These slides were then stained with rabbit primary antibody (anti-mouse CD11b Antibody from Novus Biologicals or anti-mouse CD11c Antibody from Cell signaling) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse, Biolegend cat. 901514; rabbit, Cell Signaling 3724S), and two PLA probes (anti-rabbit-PLUS ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with a rabbit polyclonal anti‐pERK antibody (Cell Signaling; 9101L, lot #30, 1:1000), and mouse anti-NF200 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... with HRP-conjugated secondary antibodies (Goat Anti-Rabbit Immunoglobulins/HRP, Dako P0448; Horse Anti-Mouse IgG, HRP-linked, Cell Signaling Technology #7076). BCIP/NBT substrate was used to detect ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed in TBST and then incubated for 45 min at room temperature in blocking buffer containing either anti-rabbit or anti-mouse HRP-linked secondary antibody (Cell Signaling Technology). Membranes were washed in TBST and imaged on the ChemiDoc Imaging System using SuperSignal chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Genetics 2022Quote: ... followed by 1 h incubation in horseradish peroxidase–conjugated secondary antibody (anti-rabbit or anti-mouse, #7074 and #7076, Cell Signaling Technology) diluted in PBS-T 5% milk at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The pSrc/Src ratio was assessed with a rabbit Phospho-Src Family (Tyr416) Antibody (#2101, Cell Signaling Technology, Danvers, MA, USA; RRID:AB_331697), recognizing the phosphorylation at tyrosine 424 in murine Src ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... before incubating overnight at 4°C at 1:100 in blocking solution for all primary antibodies used: rabbit anti-E-Cadherin (24E10 – Cell Signaling 3195), rabbit anti-Piezo1 (Novus NBP-78537) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then incubated with the appropriate secondary HRP-linked antibody as follows: anti rabbit-HRP (1:3000, Cell Signaling Technology 7074) for NEMO ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed in TBS-BSA and incubated overnight on a shaker in TBS-BSA containing Rabbit anti-cFos (1:2,000; Cell Signaling Product# 2250).
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bioRxiv - Pathology 2023Quote: ... They were then incubated first with blocking solution for 1 hr and then with a rabbit polyclonal anti-RelA antibody (8242; Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used in this study: rabbit anti-cleaved Dcp-1 (Asp215) (1:250, Cell Signaling, Danvers, MA, USA), rabbit anti-peroxidasin (Pxn ...
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bioRxiv - Neuroscience 2023Quote: ... Corresponding secondary antibodies conjugated with horseradish peroxidase (cat no: 7074 Rabbit, cat no: 7076 Mouse, cat no: 7077 Rat, Cell Signaling Technology) in combination with enhanced chemiluminescence reagent (cat no ...
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bioRxiv - Neuroscience 2023Quote: ... TBR2+ cells were determined in comparison of each cell population measured against immunoreactivity of a Rabbit IgG isotype control (Cell Signaling, #5472). For all measures ...
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bioRxiv - Genomics 2023Quote: ... The membrane was washed five times with TBST and then incubated for 1 h with secondary goat-anti-rabbit-HRP conjugate (1:4000, Cell Signaling Technology) in TBST with 5% non-fat powdered milk ...
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bioRxiv - Cell Biology 2023Quote: ... and stained 1h/RT or overnight/4°C with following primary antibodies in 0,2% Triton-X100 in PBS: PAX6 (rabbit, CS60433, Cell Signaling, 1:300), ZO-1-1A12 (mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... for ZIP4 or an HRP-conjugated anti-rabbit immunoglobulin-G for β-actin at 1:5000 dilution (Cell Signaling Technology, Catalog# 7074S) by chemiluminescence (VWR) ...
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bioRxiv - Neuroscience 2023Quote: ... were from Thermo Fisher Scientific (Waltham, MA). Rabbit monoclonal antibody to Flotillin-1 (cat. 3253) and Alix (cat. 2171) were from Cell Signaling Technology (Danvers, MA). Mouse monoclonal antibody to CD31 (cat ...
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bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Genetics 2023Quote: ... The following antibodies and reagents were purchased and used in this research: Rabbit anti-cleaved Drosophila Dcp-1 (Asp216) antibody from Cell Signaling Technology, Inc ...
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bioRxiv - Developmental Biology 2023Quote: ... Remove buffer and replace with 100μL DBE supplemented with primary antibody of interest (data presented here: rabbit anti-H3K27Me3 antibody (Cell Signaling, 1:100). Incubate cells plus antibody angled sideways on orbital shaker ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody incubation was followed by incubation in HRP-conjugated secondary antibody for 1h RT at room temperature (goat anti mouse IgG and goat anti-rabbit IgG, Cell Signaling Technology). Bands were detected by chemiluminescence using ECL solution (WesternBright Sirius ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were blocked in 0.5% BR (Blocking Reagent (Akoya Biociences))/TNT (TN, 0.05% Tween) for 1h at RT and incubated with a primary antibody against pSMAD1,5,9 (Cell Signaling 13820S, rabbit, 1:300) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The blots were then incubated with a peroxidase-conjugated anti-rabbit or anti-mouse IgG antibody (1:2000) (# 7074 or #7076, respectively; Cell Signaling Technology). The antibody–antigen reactions were detected using the Clarity™ or Clarity™ MaxWestern Blotting ECL Substrates (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were then washed and incubated for 1 h in goat anti-rabbit HRP conjugated secondary antibody (Cell Signaling Technology, 7074) diluted 1:3000 in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... These steps were followed by the incubation with primary antibodies overnight at 4°C in a solution of 2% NGS in PBS 1x: chicken anti-mCherry (1:1000, HenBiotech, Portugal) and rabbit anti-NeuN (1:100, Cell Signaling, USA), rabbit anti-Iba1 (1:750 ...
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bioRxiv - Cancer Biology 2023Quote: ... probed with primary antibodies (Supplementary Table 3) and detected with horseradish peroxidase-conjugated α-rabbit or α-mouse secondary antibodies (Cell Signaling). The blots were imaged using a ChemiDoc MP Imaging system (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were incubated in rabbit anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody (1:200, catalog # 3678, Cell Signaling Technology) in 1% BSA/PBST overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling, #3724, 1:500 dilution), in combination with one of four primary guinea pig antibodies against synaptic markers ...
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bioRxiv - Neuroscience 2023Quote: ... Double labeling for phospho-c-Jun and TH was performed with phospho-specific rabbit monoclonal antibody (Cell Signaling, cat.# 3270; 1:500) for c-Jun and sheep anti-TH antibody (Novex Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... c-Jun phosphorylated at the JNK phosphorylation site Ser73 was detected with phospho-specific rabbit antibody (Cell Signaling, cat.# 3270; 1:1,000) and the total c-Jun – with mouse monoclonal antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... Western blots were performed using rabbit monoclonal antibody against Abcb1 (clone E1Y7S) and rabbit polyclonal antibody against Lrp1 (Cat.-No.: 64099) (both antibodies were bought from Cell Signaling Technology), as well as rabbit polyclonal antibody against claudin-5 (Cat.-No. ...