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Citations for Cell Signaling Technology :
8601 - 8650 of 9030 citations for GAPDH Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies (0.25% Triton-X, 2.5% normal goat serum, PBS; 24h) against Fos (1:800 rabbit anti-cFos, Cell Signaling #2250; RRID: AB_2247211) or tyrosine hydroxylase (1:400 mouse anti-TH ...
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bioRxiv - Developmental Biology 2021Quote: ... and Samples were washed extensively in PBS-Tx (6 × 1 hour) and incubated with anti-rabbit-HRP (Cell Signaling #7074, 1:1000) diluted in 1% BSA/PBS-Tx ...
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bioRxiv - Systems Biology 2021Quote: ... we washed the cells 5 X 5 min with 5% BSA-PBS and then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling, 8889). After the secondary incubation ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence staining was performed by a standard protocol using an anti-HA rabbit primary antibody (C29F4, Cell Signaling Technology, diluted 1:1600) and a secondary antibody goat anti-rabbit Alexa Fluor 633 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... Specific proteins were detected using 1:1000 dilution of Myc-Tag (9B11) mouse monoclonal and proliferating cell nuclear antigen (PCNA, D3H8P) XP Rabbit monoclonal primary antibodies (Cell Signaling Technology). Following primary antibody incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the following antibodies were used anti-Bassoon (1:400, SAP7F407; mouse monoclonal; Enzo Life) and anti α-synuclein (1:500, D37A6; rabbit polyclonal; Cell Signaling). Species-specific secondary antibodies coupled to Alexa 488 and 350 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... MAPK activity was assessed using phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) primary antibody (1:200, rabbit, Cell Signaling Technologies #9101), total p44/42 MAPK (ERK1/2 ...
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bioRxiv - Pathology 2021Quote: ... The separated proteins were transferred onto a nitrocellulose membrane for detection with polyclonal rabbit anti-Phospho-Myosin Light Chain 2 (Ser19) antibody (Cell Signaling Technologies) or monoclonal rabbit anti-Myosin Light Chain 2 (D18E2 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were immunoprecipitated from precleared lysates overnight at 4°C using 4 μg of control IgG (rabbit or mouse, Cell Signaling Technology) or indicated antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... ERK1/2 (#9102, Rabbit, WB 1:1000) and MEK1/2 (#4694, Mouse, WB 1:1000) antibodies were purchased from Cell Signaling Technology, MA ...
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bioRxiv - Cell Biology 2020Quote: ... and two different YAP1 antibodies were used to characterize YAP1 expression patterns: mouse monoclonal anti-YAP1 antibody (catalog # sc-101199,1:50) and rabbit monoclonal anti-YAP1 antibody (catalog # 14074S, Cell Signaling,1:100). To prevent nonspecific binding of the antibodies ...
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated at 4°C overnight with the following primary antibodies: rabbit anti-ubiquitin (1:1000; Cell Signaling Technology no. 3933), rabbit anti-phospho-eIF2α (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... To detect phosphorylated ERM proteins a rabbit anti-phospho–ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (pERM) antibody was used (Cell Signaling Technology). Isotype control staining was performed using rabbit IgG isotype mAb Alexa Fluor 647 (DA1E ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were labelled overnight with primary antibodies diluted in PBS 10% (w/v) BSA (for anti-RelA: rabbit monoclonal, Cell Signaling Technology #8242 ...
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bioRxiv - Immunology 2020Quote: ... Blots were then incubated in HRP-linked secondary antibody against mouse or rabbit IgG (1:3000, Cell Signaling, Cat# 7074S and 7076S) in 4% BSA for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Depletion of ATGL16L1 and LC3B was verified on ALI 7 days by immunoblot analysis using antibodies to rabbit anti-ATG16L1 (1:1000 vol;vol ATG16L1 1:1000 vol;vol (Cell Signaling D6D5) or rabbit anti-LC3B (1:500 vol;vol Sigma-Aldrich L7543) ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed and processed for immunofluorescence as described above with a rabbit ɑ-FLAG antibody (1:1000, Cell Signaling Technologies, D6W5B) to visualize BicD2-KIF fusions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody solution contained a 1/1000 dilution of a monoclonal rabbit anti-HA antibody (C29F4, Cell Signaling Technology, Danvers, USA) in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The immunoblots were then incubated with secondary antibody for 1 h at room temperature (1:1000 dilution, anti-rabbit Cat. #7074, lot 28, or anti-mouse HRP-conjugate Cat. #7076S, lot 32, Cell Signaling Technology). Specific signal was detected using SuperSignal West Pico Chemiluminescent Substrate (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were washed with Tween-TBS and incubated in secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076; both Cell Signaling technology) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Poly-ADP-ribose polymerase (PARP) antibody and goat anti-rabbit IgG secondary antibody conjugated to horseradish peroxidase (HRP) were acquired from Cell Signaling Technology-Ozyme (Saint-Quentin-en-Yvelines ...
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bioRxiv - Biochemistry 2022Quote: ... Unroofed RBCs were blocked for 30 mins at room temperature in blocking buffer and then incubated with 1:200 rabbit anti-HA primary antibody (3724; Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Immunology 2022Quote: ... primary antibody pairs against NFκB total (rabbit-anti-NFκB p65 total, Cell Signaling Technology #8242; mouse-anti-NFκB p65 total, Cell Signaling Technology #6956); NFκB phospho (rabbit-anti-NFκB p65 total ...
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bioRxiv - Immunology 2022Quote: ... The following horseradish peroxidase (HRP) conjugated secondary antibodies were used for this study: mouse anti-rabbit IgG (L27A9) monoclonal antibody (Cell Signaling #5127), goat anti-rabbit IgG polyclonal antibody (Cell Signaling #7074S) ...
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bioRxiv - Microbiology 2022Quote: ... The primary antibody was diluted to 0.1 mg/mL and anti-rabbit IgG HRP linked secondary antibody diluted 1:1000 (Cell Signaling Technology; 7074S).
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bioRxiv - Immunology 2022Quote: ... and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour, Cell Signaling Technology 7074S) with the peroxidase substrate KPL TrueBlue (30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 8 min at 37 °C with primary antibodies against HA (rabbit, 1:250, Cell signaling Technology, cat #C29F4). After washes ...
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bioRxiv - Physiology 2023Quote: ... Bound primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse or -rabbit secondary antibodies (Cell Signaling Technology, Inc., Danvers, MA), and protein signals were visualized using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T). The negative control sample used ΔKu80 parasites (the parental line ...
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bioRxiv - Immunology 2024Quote: ... then stained at 4°C overnight with various combinations of the following antibodies: rabbit anti-NeuN (1:1000, Cell Signaling Technologies, 12943S), rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... The membranes were subsequently washed 4 times in TBS-T for 10 min before incubating in the appropriate secondary antibody anti-rabbit HRP (1:5000; 1677074P2, Cell Signaling Technology) or anti-mouse HRP (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins captured on the magnetic beads were eluted with 2X SDS loading dye for 5 min and analyzed by Western Blotting using rabbit anti-FLAG (14793S; Cell Signaling Technology) and anti-GFP (A-6455 ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-phospho-p70 S6 kinase (Thr389) (clone 108D2, #9234) and anti-LC3A/B (clone D3U4C, #12741) were rabbit monoclonal antibodies from Cell Signaling Technology. Anti-Akt (#9272 ...
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bioRxiv - Neuroscience 2022Quote: ... One set of tissue sections from each rat (with each set containing a complete rostrocaudal series of sections spaced by 210 μm) was incubated in a rabbit polyclonal antiserum against cFos (1:10,000; Cell Signaling, 2250; AB_2247211), followed by biotinylated donkey anti-rabbit IgG (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-Rabbit Secondary Antibody in 1:30000 dilution and Anti-Mouse Secondary Antibody (Cell Signaling Technology, 5151; Li-Cor, 926-68020, respectively) were used in 1:15000 dilution during Western Blotting experiments.
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bioRxiv - Biophysics 2022Quote: SnapTag and HaloTag Janelia Fluor dyes were obtained from the Janelia Research Campus.54 Rabbit monoclonal antibodies against EEA1 and against LAMP1 were from Cell Signaling Technologies (Catalog # ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were washed and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody generated in Rabbit (1:2000; Cell Signaling Technology #7074) for 1.5 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibody solution contained goat anti-mouse antibodies FITCI at 1:1000 dilution and goat anti-rabbit dilution TRITCI at 1:1000 dilution (from Cell Signaling Technologies) and incubated in humidity chambers at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 488-conjugated rabbit monoclonal anti-phospho-histone H3 (pH3) (1:200 for IF and 1:50 for flow cytometry; D2C8; Cell Signaling Technology); and fluorescence (Alexa Fluor 488 or Alexa Fluor 594 ...
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bioRxiv - Cancer Biology 2024Quote: ... 4×106 cells were fixed in methanol and incubated with anti-pS6 (Ser235/236) rabbit antibody (1:100, Cell Signaling Technology #2211), followed by incubation with cell surface markers and secondary Alexa Fluor 647 goat anti-rabbit (1:200 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were diluted in PBS-T containing 5% BSA and used 1:1000 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058), 1:1000 for anti-phospho-Akt(Thr308 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in primary antibodies (1:500 HCS-1 mouse monoclonal, DSHB, University of Iowa; 1:400 anti-cleaved Caspase-3 rabbit polyclonal, Cell Signaling Technology). After overnight incubation in primary ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary and secondary antibodies were diluted in FACS buffer and used 1:100 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058) and 1:500 for anti-rabbit Alexa-647 (goat ...
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bioRxiv - Cell Biology 2024Quote: ... Specimens were incubated overnight at 4 °C with rabbit anti-SARS-CoV-2 nucleocapsid protein (Cell Signaling Technology, USA, #26369, 1:200), mouse anti-ACE2 (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP, 7076; Cell Signaling) for 1 h at room temperature ...