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Citations for Cell Signaling Technology :
8801 - 8850 of 9030 citations for GAPDH Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with anti-human N-Myc rabbit monoclonal antibody (#51705, Cell Signaling Technology, Inc., MA, USA at a dilution of 1:300) and S100 beta (EP32 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation for 1 hour at room temperature in the dark in with secondary antibodies (Anti-rabbit: IgG DyLight 800, Anti-mouse: IgG DyLight 680 (Cell Signaling Technologies, USA)) ...
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bioRxiv - Genetics 2024Quote: ... Horseradish peroxidase(HRP)–conjugated streptavidin (streptavidin-HRP; Cat# 3999S) and HRP-conjugated rabbit monoclonal antibody against β-actin (Cat# 5125S) were obtained from Cell Signaling Technology. HRP-conjugated mouse monoclonal antibody against FLAG was obtained from GenScript (Cat# A01428).
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bioRxiv - Biochemistry 2023Quote: ... were used at a concentration of 1:1000 and visualized using HRP-conjugated secondary antibodies (anti-rabbit [Cell Signaling, 7074S] or anti-mouse [Cell Signaling, 7076S]) at a concentration of 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using goat anti-mouse or anti-rabbit IgG (H+L) Alexa fluor 488 or 647 (Cell signaling Technologies/ Bioconcept) secondary antibodies diluted 1:500 in 1% BSA 0.1% Triton-X 100 in PBS for 3 hours in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... β-actin [8H10D10] primary antibodies and anti-rabbit-HRP or anti-mouse-HRP antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). IRF3 [A19717] were purchased from ABclonal (Woburn ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... To confirm the specificity of staining cells were incubated with isotype mouse (7076P2) or rabbit (7074P2) IgG antibodies (Cell Signaling, Danvers, MA, USA) (Fig ...
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bioRxiv - Immunology 2023Quote: ... Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies: goat anti-rabbit (1:2000, Cell Signaling Technology, Cat. 7074), goat anti-rat (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Primary staining was performed on separate blots with the following antibody dilutions in 5% BSA/TBST: rabbit anti-EZH2 1:1000 (Cell Signaling Technology, 5246S), rabbit anti-CBX2 1:500 (Bethyl Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were washed in TBS containing 0.1 % Tween20 and labeled with horseradish peroxidase (HRP)-conjugated secondary anti-mouse or anti-rabbit antibody (Cell Signaling Technology, Beverly, MA). Proteins were enhanced by chemiluminescence (Amersham ECL plus Western Blotting detection system ...
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bioRxiv - Cell Biology 2022Quote: ... the following primary antibodies were used: rabbit polyclonal anti-phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) at 1:250 (Cell Signaling Technology, #3141), rabbit polyclonal anti-phospho-Myosin Light Chain 2 (Ser19 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... anti-Rab11 (1:50; CST, C45B10, #3288) or anti-LAMP1 (1:100; CST, D2D11 XP, #9091) rabbit monoclonal antibodies were purchased from Cell Signaling Technology (CST). Alexa 488 goat anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... and processed for indirect immunofluorescence analysis according to standard protocols using phospho-Histone H2A.X (S139) (20E3) Rabbit antibody (Cell Signaling Technology, 1:1000) and Alexa Fluor 647 goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... one slide was incubated only with Alexa Fluor® 488-conjugated Myc-Tag (71D10) rabbit monoclonal antibody purchased from CST (Cell Signaling Technology). For the measurements ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse-HRP, Cell Signaling #7076; anti-rabbit-HRP, Cell Signaling #7074) in blocking solution (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were washed three times with PBST and incubated with either 1 μg/ml of rabbit monoclonal anti-mouse TREM2 (Cell Signaling Technology, 76765) for full length Trem2 ELISA (ELISA 1) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 2% milk-phosphate-buffered saline (PBS) and incubated with the following antihuman antibodies: rabbit β3-integrin (1:1000; 13166, Cell Signaling Technology [CST]), rabbit p-FAK (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were treated with an HRP-linked secondary antibody (Anti-rabbit IgG cat. no. 7074S or Anti-mouse IgG cat. no. 7076S; Cell Signaling Technology, Inc) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS−/−] at room temperature for 1.5 h and subsequently incubated with primary rabbit anti-MPO antibody (Cell Signaling, clone: E1E7I, 1:500) and primary mouse anti-HLA-G antibody (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit anti-SAPK/JNK monoclonal antibody (Cat# 9258, RRID: AB_2141027), and rabbit anti-p-JNK monoclonal antibody (Cat# 4668, RRID: AB_823588) were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-ATF2 monoclonal antibodies (Cat# BS1022 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were permeabilized with 0.5% Triton-X in PBS before probing with anti-phospho-histone H2AX (Ser 139) rabbit monoclonal antibody (Cell Signaling Technology cat# 9718) and anti-cyclin A2 (EPR17351 ...
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bioRxiv - Bioengineering 2023Quote: ... Paraffin sections were used for H&E staining as well as sequential double-immuno-staining with rabbit anti-human Ki67 (Cell Signaling Technologies, US) and rabbit anti-human vimentin (Thermo Fisher Scientific ...
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Specific patterns of neural activity in the hippocampus after massed or distributed spatial trainingbioRxiv - Neuroscience 2023Quote: ... After 1 h of incubation in PBST containing 1% BSA and 1% NGS sections were incubated overnight in anti-phospho-c-Fos rabbit monoclonal antibody (5348S; Cell signaling Technology, USA) diluted 1:8000 in PBST– BSA–NGS ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was blocked for 1 h at room temperature with 5 % BSA dissolved in TBS-Tween (0.1 %) and incubated with the primary antibody rabbit anti-phospho-threonine [(Cell Signaling Technology®, #9381) 1:1000] ...
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bioRxiv - Immunology 2023Quote: ... blots were also probed with an HRP-labelled rabbit anti-ß-actin monoclonal antibody at a 1:1000 dilution (#5152; Cell Signaling Technology). Western blots were visualized with SuperSignal West Pico Chemiluminescent reagents (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Cells were proceeded for IF as described above using anti-CD56 mouse antibodies (1:10, Coulter #PN4235479-G) and anti-TCF7L2 rabbit antibodies (1:100, Cell Signaling Technology #C48H11), revealed by Cy3-coupled anti-mouse IgGs and Cy5-coupled anti-rabbit IgGs.
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used at the indicated dilutions: rabbit polyclonal anti-phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) at 1:50 (Cell Signaling Technology, #3141), rabbit anti-Msn M1 at 1:750 (gift from Thomas Moss ...
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bioRxiv - Cancer Biology 2023Quote: ... These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology, USA) secondary antibodies conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076, Cell Signaling, Goat anti-rabbit 7074, Cell Signaling) for two hours with 2% milk powder in TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were rat anti-ATF4 (1:200, (Hunt et al., 2019)) and rabbit anti-P-eIF2α (1:500, anti-Phospho-eIF2α [Ser51], Cell Signaling Technology 9721). Secondary antibodies were goat anti-rabbit Alexa Fluor 546 (Invitrogen A11035 ...
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bioRxiv - Immunology 2023Quote: ... The membranes were then washed three times with TBST and incubated with HRP-linked anti-rabbit IgG antibody (Cell Signaling Technology, catalog # 7074) in TBST containing 5% BSA for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with peroxidase-conjugated secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody, Cell Signaling #7074; Anti-mouse IgG, HRP-linked Antibody, Cell Signaling #7076) and developed with a luminol-based enhanced chemiluminescence (ECL ...
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bioRxiv - Bioengineering 2023Quote: ... secondary antibodies were incubated for 1 hour at room temperature (Secondary antibodies: Anti-Rabbit HRP-conjugated Cell Signaling Technology (CST-#7074S 1:5000); Anti-Mouse HRP-conjugated (R&D ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using rabbit-α-Shh antibodies (Cell signaling C9C5), rabbit-α-GAPDH antibodies (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated overnight at 4 °C with rabbit anti-c-fos primary antibody (1:500; Cell Signaling Technology, catalog no. 2250, USA) in a permeable buffer containing 2% donkey serum ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were incubated with the appropriate primary antibody overnight followed by horseradish peroxidase-conjugated anti-rabbit (#7074, Cell Signaling Technology, Danvers, MA) secondary antibody at a dilution of 1:2500 for an hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The sections were washed twice with PBS and incubated in 2% BSA plus TBS with primary antibody [1:80 dilution rabbit anti-5-mC antibody (Cell Signaling Technology, #D3S2Z) at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... and the mouse anti-rabbit (#3678S) and the anti-mouse HRP-conjugated secondary antibodies (#7076S) were from Cell Signaling Technology (Beverly, MA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... and immunoblotting experiments were carried out on 10 μL of resultant IP preps whereby polyubiquitinated MyHC fragments were probed using a polyclonal rabbit IgG antibody (1:1000; Cell Signaling; cat#: 3933). In addition to these IP experiments ...
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bioRxiv - Genomics 2024Quote: ... with primary antibody (0.25 µL polyclonal rabbit αHP1 [11]; 0.5 µg αH3K9me3, Abcam 8988; 0.5 µL αBiotin (Cell Signaling Technology # D5A7; for DiBioCUT&Tag) or 0.5 µg normal rabbit IgG (MERCK ...
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bioRxiv - Immunology 2024Quote: ... Vero cells were also infected with RSV-mkate2 at an MOI of 0.1 for 48h in the presence of either rabbit IgG (Cell signaling, catalog no. 2729) or IgG antibodies against ISG15 ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid-bound protein was run on 4-20% SDS-PAGE gel and immunoblotted with His-tag antibody (rabbit monoclonal, Cell Signaling Technology, 2365). Blots were visualized on a Li-Cor Odyssey imaging system ...
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bioRxiv - Neuroscience 2024Quote: ... All sections were incubated overnight at 4⁰ C with rabbit anti-c-Fos primary antibody (5348, 1:2000; Cell Signaling Technology, RRID: AB_10557109) diluted in 2% NHS in PBS-Tx.
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bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...
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bioRxiv - Molecular Biology 2020Quote: ... the membranes were washed and incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:3000, Cell Signaling Technology, #7076 or #7074) and followed by detection with ECL Western blot substrate (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: The following primary and secondary antibodies were used for immunoblotting: Anti-EGFR rabbit antibody (no. 4267, Cell Signaling Technology, Danvers, MA, 1:1,000 dilution); anti-phospho-EGFR (Tyr1068 ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...