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Citations for Cell Signaling Technology :
8451 - 8500 of 9030 citations for GAPDH Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h and incubated overnight at 4°C with the corresponding primary antibody diluted in blocking solution: Anti-Caspase 3 (rabbit, 1:100, Cell Signaling). The next day ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with PBS-0.1% Tween 20 (PBS-T) containing 5% milk and were incubated with rabbit monoclonal anti-CYLD (1:500, #8462 Cell Signaling) and mouse monoclonal anti-β-actin (1:5,000 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Primary antibodies/concentrations used were as follows: rabbit anti-phospho-S6 ribosomal protein (Serine240/244) (1:100, Cell Signaling Technologies, #2215), rabbit anti-phospho-S6 ribosomal protein (Serine244/247 ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to nitrocellulose membrane (Cytiva) and revealed using following antibodies: rabbit AGR2 (Cell Signaling Technologies, Clone D9V2F, 1/1000), mouse FLAG-M2-HRP (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were blocked using 5% BSA and 5% donkey serum in PBS containing 0.3% Triton X-100 and incubated overnight at 4 °C with a combination of the following antibodies and stains: rabbit anti-pMLC Thr18/Ser19 (1:100, catalog no. 3674; Cell Signaling); mouse anti- CASPR (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immunolabeling was realized using the rabbit monoclonal primary antibody anti-phospho-ERK1/2 Thr202/Tyr204 (#4370, Cell Signaling Technologies, 1:2,000), or anti-total ERK1/2 (#05-1152 ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody was either goat anti-rabbit (7074) or horse anti-mouse (7076) IgG conjugated with horseradish peroxidase (Cell Signaling Technology). Staining was developed with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... They were then incubated with a rabbit anti-phosphomyosin S19 antibody (1:200 dilution, #3671, Cell signaling technology, Danvers, Ma, USA) diluted in PBS with horse serum and 0.01% Triton at 4C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated in HRP-linked anti-mouse or anti-rabbit IgG antibody for 1h at 24°C with gentle rocking (1:5,000 dilution, Cell Signaling Technology). Membranes were washed in TBST and protein bands were visualized using the Pierce ECL detection system (Cat #32106 ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:20000 (to detect GluN2C or GluN2A) or HRP-conjugated goat anti-rabbit IgG secondary antibody (Cell Signaling, cat. #7074) at 1:40000 (to detect β-actin ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated with horse radish peroxidase-linked anti-mouse or anti-rabbit secondary immunoglobulin antibodies (1:10000; Cat# 7076S, Cat# 7074S Cell signaling) in PBST for 40 mins ...
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bioRxiv - Cell Biology 2023Quote: ... to the following proteins were used for the immunodetection of proteins using horseradish peroxidase-conjugated anti-mouse (Cytiva, NA931) and anti-rabbit (Cytiva, NA934) secondary antibodies (1:1,000): CHOP (Cell Signaling, 5554), Cleaved Caspase-3 (Cell Signaling ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-phospho-histone H3 (Ser10) to detect cells at the G2/M phase of cell cycle (H3P, Cell Signaling Technology) diluted 1:300 ...
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bioRxiv - Biochemistry 2023Quote: ... β-actin levels were detected using a rabbit anti-β-actin antibody at 1:5000 dilution (Cell Signaling Technology, Catalog# 4970S). Primary antibodies were detected with an HRP-conjugated anti-mouse immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used in meningeal whole mount and cleared tissue staining: rabbit anti-Desmin (Cell Signaling, Cat#: D93F5), rat anti-CD31/PECAM (BD Pharmigen ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the following primary antibodies: rabbit anti-c-Fos (1:3000, RRID:AB_2247211, Cell Signaling Technology, USA), mouse anti-NeuN (1:800 ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-phospho phospholamban (Ser16/Thr17) antibody (#8496) and rabbit anti-phospho troponin I (Ser23/24) antibody (#4004) are from Cell Signaling. Rabbit anti-phospho ryanodine receptor 2 (Ser2808 ...
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Western blot analysis was carried out as described (60) using antibodies specific for rabbit EZH2 (Cell signaling technology, #5246, 1:1000), rabbit Amylase (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were labeled with mouse anti-GFP (1:500) antibody and rabbit anti-FOXP1 antibody (Cell Signaling Technology; 1:400) and detected by biotinylated secondary antibody/streptavidin-Alexa Fluor 488 (GFP ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membrane was shaken three times for 5 min in TBST then incubated with 1:3,000 anti-rabbit HRP-linked antibody (Cell Signaling Technologies) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C with the primary c-Fos antibody (1:1000, Rabbit, Cell Signaling #2250). After 3 rinses with PBS for 10 min ...
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bioRxiv - Genetics 2024Quote: ... primary antibodies were diluted in 2.5% skimmed milk as follows: 1:1000 anti-rabbit phospho-eEF2 (Thr56) antibody (#2331, Cell Signaling Technologies), 1:1000 anti-rabbit eEF2 antibody (#2332 ...
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bioRxiv - Neuroscience 2023Quote: ... a secondary reaction was performed by simultaneously incubating the sections with a rabbit antibody directly conjugated to Alexa Fluor 488 (1:500; catalog #4412S, Cell Signaling) for CR and with a guinea pig antibody directly conjugated to Alexa Fluor 647 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was blocked in 5% dry milk in TBS-T for 30 min and incubated overnight at 4°C with primary antibodies against: rabbit V5 at 1:1,000 (Cell Signaling Technology), mouse vinculin at 1:1,000 (Millipore Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... The commercial antibodies used in this study included a GFP (D5.1) XP Rabbit monoclonal antibody (Cell Signaling Technology, Danvers, MA, USA), GAPDH (glyceraldehyde-3-phosphate dehydrogenase ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with a respective secondary HRP-conjugated antibody (anti-rabbit: Cell Signaling Technologies, #7074S, anti-mouse: Cell Signaling Technologies, #7076) for one hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Overexpression of Flag-NUDT15 and HA-NUDT18 were confirmed by analyzing cell lysates by SDS-PAGE followed by Western blotting using anti-FLAG-tag Rabbit antibody (#740001, Thermo Fischer Scientific) and anti-HA-tag (6E2) Mouse antibody (#2367, Cell Signaling) followed by anti-Rabbit HRP or anti-Mouse HRP ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 5X with cold TTBS and incubated with anti-Rabbit or anti-mouse secondary antibodies conjugated to HRP (#7074 and #7076, Cell Signaling). After washing ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed two times with PBS and incubated with 50 µL of primary antibody (α-LAMP1 rabbit, Cell Signaling, 1:100 in 1 % BSA ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked with 2% BSA in PBS for 1 hour at room temperature before antibody staining with rabbit ⍺-Sec61b (Cell Signaling) and mouse ⍺-Tom20 (Santa Cruz ...
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bioRxiv - Neuroscience 2023Quote: ... immunoblots were probed for anti-rabbit acetylated histone 3 at lysine 9 and histone 4 at lysine 5 (ac-H3K9 and ac-H4K5 respectively, Cell Signaling), anti-rabbit LC3B (Abcam) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were incubated overnight at 4°C with rabbit anti-HA antibody (1:1000, catalog #3724S, Cell Signaling, Danvers, MA) diluted in 5% bovine serum albumin ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were next incubated overnight at 4 °C with Shh (C9C5) rabbit antibody (1:500, Cell Signaling Technologies, Cat. No. 2207) in PBS-BSA-T ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Membranes were then incubated with rabbit monoclonal anti-CtIP antibodies (1:3000 dilution in TBS-T; #9201, Cell Signaling Technology, USA), rabbit monoclonal anti-cleaved caspase-3 (1:3000 dilution in TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were anti-rabbit and anti-mouse IgG HRP-linked antibodies (1:5000, Cell Signaling, 7074 and 7076 respectively). Images were developed using the MINI-MED 90 X-Ray Film Processor (AFP Manufacturing Corp) ...
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibodies used in this study were as follows: rabbit anti-DNMT3B (1:2000; 57868, Cell Signaling Technology, Massachusetts, USA), rabbit anti-PLCG2 (1:2000 ...
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bioRxiv - Physiology 2024Quote: Mouse retinae were fixed in 4% formaldehyde in PBS for 1h and wholemount immunostained as described [31] using rabbit anti-P-p38 MAPK (Thr180/Tyr182) antibody (Cell Signaling) followed by Alexa Fluor 555–conjugated goat anti–rabbit antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with horseradish peroxidase-conjugated secondary IgGs (1:1,000 for rabbit #170-6515, mouse #170-6516, Bio-Rad, CA or 1:2,000 for #7076s, Cell signaling, MA), for 1hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were visualized after staining with the appropriate goat anti-rabbit conjugated to Alexa-488 (1:500, Cell Signaling, 4412) and and donkey anti-chicken IgG conjugated to Alexa-647 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 h blocking with 15% milk powder (in TBST) and overnight incubation with rabbit anti-BIP (3183, Cell signaling, 1/1000 for retina ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times and incubated with anti-mouse IgG or anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technology) both diluted in PBST (1:2,000) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted by heating Protein-G agarose beads and anti-HA magnetic beads for 15 min at 42°C in 50μL 2X sample buffer and analyzed by western blotting using rabbit anti-HA (1:1000, Cell Signaling Technology) and mouse anti-FLAG (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The following primary antibodies and dilutions were used in this study: rabbit anti-G9a/EHMT2 (C6H3) (Cell Signaling, 3306T, 1:200), anti-histone H3K9me3 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... the blots were washed in TBS-0.05% tween-20 and incubated with a secondary antibody HRP-conjugated Goat anti-Rabbit IgG (Cell Signaling Technology). The anti-actin antibody was directly conjugated to HRP ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were stained overnight with 13.1 mice monoclonal anti-P28 (1:1000) and rabbit anti-HA (1:250, Cell Signaling Technologies) antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed three times for five minutes with TBS-T and incubated for one hour with 1:15,000 HRP-linked anti-rabbit or anti-mouse antibodies (Cell Signaling, 7074S, 7076S) or 1:15,000 DyLight anti-rabbit IgG 800 conjugate (Cell Signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... Blots were washed three times (total 30 min) and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature with gentle rocking ...