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• Multiscale networks in multiple sclerosis

  Keith E. Kennedy, et al., bioRxiv - Systems Biology 2023

  Quote: ... and Tube 3: mouse monoclonal anti-human HSP27 (Clone G31, catalog # 2402; Cell Signaling Technology) and rabbit polyclonal anti-human Phospho-HSP27 (Catalog #AF2314 ...
• Retrospective identification of intrinsic factors that mark pluripotency potential in rare somatic cells

  Naveen Jain, et al., bioRxiv - Systems Biology 2023

  Quote: ... 1:1000 rabbit anti-beta actin (Cell Signaling Technology #4970S), and 1:2000 rabbit anti-histone H3 (Abcam #AB1791) ...
• Uptake-independent killing of macrophages by extracellular aggregates of Mycobacterium tuberculosis is ESX-1 and PDIM-dependent

  Chiara Toniolo, Neeraj Dhar, John D. McKinney, bioRxiv - Microbiology 2023

  Quote: ... phospho-RIP3 (Cell Signaling), phospho-MLKL (Cell Signaling) ...
• Uptake-independent killing of macrophages by extracellular aggregates of Mycobacterium tuberculosis is ESX-1 and PDIM-dependent

  Chiara Toniolo, Neeraj Dhar, John D. McKinney, bioRxiv - Microbiology 2023

  Quote: ... phospho-MLKL (Cell Signaling), mouse CD45.1-AlexaFluor647 (BioLegend ...
• Neuronal expression of herpes simplex virus type-1 VP16 protein induces pseudorabies virus escape from silencing and reactivation by activating Jun

  Zhi-Shan Hsu, et al., bioRxiv - Microbiology 2023

  Quote: ... Anti-cJun and anti-phospho-cJun (Ser63) antibodies were purchased from Cell Signaling. Goat-anti-mouse and goat-anti-rabbit ...
• Early mitochondrial dysfunction proceeds neuroinflammation, synaptic alteration, and autophagy impairment in hippocampus of App knock-in Alzheimer mouse models

  Luana Naia, et al., bioRxiv - Neuroscience 2023

  Quote: ... PSD95 (1:1,000) (Cell Signaling, Cat. no. 3409), ULK1 (1:500 ...
• Early mitochondrial dysfunction proceeds neuroinflammation, synaptic alteration, and autophagy impairment in hippocampus of App knock-in Alzheimer mouse models

  Luana Naia, et al., bioRxiv - Neuroscience 2023

  Quote: ... p62/SQSTM1 (1:1.000) (Cell Signaling, Cat. no. 5114), Synaptophysin (1:500 ...
• Maturation of Nucleus Accumbens Synaptic Transmission Signals a Critical Period for the Rescue of Social Deficits in a Mouse Model of Autism Spectrum Disorder

  Melina Matthiesen, et al., bioRxiv - Neuroscience 2023

  Quote: ... Sections were incubated with rabbit monoclonal anti-phospho-S6S240/244 (pS6; 1:1000; Cell Signaling, Beverly, MA, RRID:AB 10694233) for 48h ...
• BAG3 regulates the specificity of the recognition of specific MAPT species by NBR1 and SQSTM1

  Heng Lin, et al., bioRxiv - Neuroscience 2023

  Quote: ... Mouse antibodies include: Myc Tag (Cell Signaling Technology, #2276), FLAG Tag (Cell Signaling Technology ...
• BAG3 regulates the specificity of the recognition of specific MAPT species by NBR1 and SQSTM1

  Heng Lin, et al., bioRxiv - Neuroscience 2023

  Quote: ... Conformation specific mouse anti-rabbit IgG (Cell Signaling Technology, #5127S). Anti-rabbit IgG HRP-conjugated Antibody (Bio-rad 51962504) ...
• BAG3 regulates the specificity of the recognition of specific MAPT species by NBR1 and SQSTM1

  Heng Lin, et al., bioRxiv - Neuroscience 2023

  Quote: ... FLAG Tag (Cell Signaling Technology, 8146), TOC1 antibody (a gift from N ...
• Astrocyte-like subpopulation of NG2 glia in the adult mouse cortex exhibits characteristics of neural progenitor cells and is capable of forming neuron-like cells after ischemic injury

  Lucie Janeckova, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-vimentin rabbit monoclonal antibody (1:200, Cell Signaling Technology, Danvers, MA, USA). Secondary antibodies were donkey/goat polyclonal anti-rabbit/mouse IgG conjugated to Alexa-488 (1:200 ...
• DRACC1, a major postsynaptic protein, regulates the condensation of postsynaptic proteins via liquid-liquid phase separation

  Takeshi Kaizuka, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-synaptophysin antibody (#4329, Cell Signaling Technology), and mouse anti-β-actin antibody (A1978 ...
• Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in Tauopathy

  Xianzun Tao, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-cleaved caspase 3 (9661, Cell Signaling), anti-LC3B (L7543 ...
• UBE3A and transsynaptic complex NRXN1-CBLN1-GluD1 in a hypothalamic VMHvl-arcuate feedback circuit regulates aggression

  Yi Nong, et al., bioRxiv - Neuroscience 2023

  Quote: 25 mg cortical tissues were dissected from wild type mice (n = 3) and chromatin was prepared using the SimpleChIP plus Enzymatic Chromatin IP Kit (Cell Signaling). UBE3A-bound chromatin was immunoprecipitated using rabbit anti-UBE3A antibody (Bethyl Laboratories) ...
• Cholecalciferol improves cognitive impairment by amending impaired insulin signaling in sporadic Alzheimer’s Disease Rats

  Tushar Kanti Das, et al., bioRxiv - Neuroscience 2023

  Quote: ... Akt (pan) and p-Akt (Ser 473) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against β-Actin was purchased from Upstate Biotechnology (Lake Placid ...
• Insights into the mechanism of oligodendrocyte protection and remyelination enhancement by the integrated stress response

  Yanan Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-XBP-1-spliced (Cell Signaling, 82914s, 1:1000), and anti-actin (Sigma ...
• ERK1/2-dependent TSPO overactivation associates with the loss of mitophagy and mitochondrial respiration in ALS

  Andrea Magrì, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-STAT3 (Cell Signaling, ref. no. 9145, 1:500), Atg12 (Cell Signaling ...
• ERK1/2-dependent TSPO overactivation associates with the loss of mitophagy and mitochondrial respiration in ALS

  Andrea Magrì, et al., bioRxiv - Neuroscience 2023

  Quote: ... Atg12 (Cell Signaling, ref. no. 4180, 1:1000), β-Actin (Cell Signaling ...
• ERK1/2-dependent TSPO overactivation associates with the loss of mitophagy and mitochondrial respiration in ALS

  Andrea Magrì, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-ERK1/2 (Cell Signaling, ref. no. 4370, 1:1000), STAT3 (Cell Signaling ...
• ERK1/2-dependent TSPO overactivation associates with the loss of mitophagy and mitochondrial respiration in ALS

  Andrea Magrì, et al., bioRxiv - Neuroscience 2023

  Quote: ... STAT3 (Cell Signaling, ref. no. 4904,1:500), phospho-STAT3 (Cell Signaling ...
• ERK1/2-dependent TSPO overactivation associates with the loss of mitophagy and mitochondrial respiration in ALS

  Andrea Magrì, et al., bioRxiv - Neuroscience 2023

  Quote: ... β-Actin (Cell Signaling, ref. no. 3700, 1:2000). Then ...
• UBE3A and transsynaptic complex NRXN1-CBLN1-GluD1 in a hypothalamic VMHvl-arcuate feedback circuit regulates aggression

  Yi Nong, et al., bioRxiv - Neuroscience 2023

  Quote: ... Sections were processed as described above for c-Fos antibody immunohistochemical staining (c-Fos Rabbit mAb #2250, 1:2000, Cell Signaling Technologies). Brain sections mounted on the slides were scanned using Zeiss Axio Scan.Z1 with 5x magnification ...
• Modeling the marmoset brain using embryonic stem cell-derived cerebral assembloids

  Tomoki Kodera, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-TBR1 (1:400, rabbit; Cell signaling), anti CTIP2 (1:500 ...
• INPP5D/SHIP1 regulates inflammasome activation in human microglia

  Vicky Chou, et al., bioRxiv - Neuroscience 2023

  Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1 ...
• INPP5D/SHIP1 regulates inflammasome activation in human microglia

  Vicky Chou, et al., bioRxiv - Neuroscience 2023

  Quote: ... Primary antibodies were prepared in 1% BSA in PBS (SHIP1, 1:100 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam) and sections were incubated overnight in this solution at 4°C ...
• INPP5D/SHIP1 regulates inflammasome activation in human microglia

  Vicky Chou, et al., bioRxiv - Neuroscience 2023

  Quote: ... Primary antibodies were prepared in blocking buffer (SHIP1, 1:100 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; ASC, 1:500 dilution, Cell Signaling Technologies; P2RY12 ...
• Distinct Transcriptomic Responses to Aβ plaques, Neurofibrillary Tangles, and APOE in Alzheimer’s Disease

  Sudeshna Das, et al., bioRxiv - Neuroscience 2023

  Quote: ... a rabbit monoclonal anti-Aβ N-terminus (clone D54D2, 1:500, Cell Signaling Technologies, Cat# 8243S), and a mouse monoclonal anti-pTau-Ser396/404 (Dr ...
• Astrocyte-like subpopulation of NG2 glia in the adult mouse cortex exhibits characteristics of neural progenitor cells and is capable of forming neuron-like cells after ischemic injury

  Lucie Janeckova, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-Mcm2 rabbit monoclonal antibody (1:800, Cell Signaling Technology, Danvers, MA, USA), anti-PCNA mouse monoclonal antibody (1:800 ...
• Insights into the mechanism of oligodendrocyte protection and remyelination enhancement by the integrated stress response

  Yanan Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-BIP (Cell Signaling, 3177s, 1:1000), anti-GADD34 (Proteintech ...
• Insights into the mechanism of oligodendrocyte protection and remyelination enhancement by the integrated stress response

  Yanan Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-T-eIF2α (Cell Signaling, 9722s, 1:1000), anti-puromycin (Millipore ...
• Neuroprotective effects of hepatoma-derived growth factor in models of Huntington’s disease

  Kerstin Voelkl, et al., bioRxiv - Neuroscience 2023

  Quote: ... and mouse anti-p-ERK (Cell Signaling Technology, 9106). The next day ...
• Presenilin-dependent regulation of tau pathology via the autophagy/proteasome pathway

  Anna del Ser-Badia, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-phosphorylated GSK3β (Ser9, 1:1000; Cell Signaling), goat anti-Akt (1:1000 ...
• A direct estrogenic involvement in the expression of human hypocretin

  Haimei Li, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-histone H3 (4620, Cell Signaling Technologies, USA), and normal rabbit IgG (2729 ...
• Presenilin-dependent regulation of tau pathology via the autophagy/proteasome pathway

  Anna del Ser-Badia, et al., bioRxiv - Neuroscience 2023

  Quote: ... total and phosphorylated (Ser235/236) S6 ribosomal protein and pULK1 (Ser757) (all 1:1000, Cell Signaling). Bands detected with secondary antibodies coupled to peroxidase and enhanced chemiluminescent reagent were captured in ChemiDoc MP System and quantified in a linear range using the ImageLab 5.2.1 software (Bio-Rad).
• A direct estrogenic involvement in the expression of human hypocretin

  Haimei Li, et al., bioRxiv - Neuroscience 2023

  Quote: ... ChIP assays were performed using a SimpleChIP Enzymatic Chromatin IP kit brought from Cell Signaling Technology (9003, USA). HeLa cells were treated with estradiol (10-5M ...
• A direct estrogenic involvement in the expression of human hypocretin

  Haimei Li, et al., bioRxiv - Neuroscience 2023

  Quote: ... and normal rabbit IgG (2729, Cell Signaling Technologies, USA) at 4□ overnight ...
• Fluoxetine treatment during postnatal and juvenile temporal epochs evokes diametrically opposing changes in anxio-depressive behaviors, gene expression, mitochondrial function, and neuronal architecture in the medial prefrontal cortex

  Utkarsha Ghai, et al., bioRxiv - Neuroscience 2023

  Quote: ... The PVDF membranes were blocked with 5% BSA and incubated with rabbit mTOR (1:1000, Cell Signaling Technologies, USA), rabbit p-mTOR (1:1000 ...
• Fluoxetine treatment during postnatal and juvenile temporal epochs evokes diametrically opposing changes in anxio-depressive behaviors, gene expression, mitochondrial function, and neuronal architecture in the medial prefrontal cortex

  Utkarsha Ghai, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit p-4EBP1 (1:500, Cell Signaling Technologies), rabbit SIRT1 (1:1000 Millipore ...
• Restoring AIBP expression in the retina provides neuroprotection in glaucoma

  Won-Kyu Ju, et al., bioRxiv - Neuroscience 2023

  Quote: Harvested retinas were homogenized for 1 min on ice with a RIPA lysis buffer (Cell Signaling Technology), containing protease inhibitor cocktail (MedChemExpress) ...
• Glucocorticoids rapidly modulate Ca_V1.2-mediated calcium signals through Kv2.1 channel clusters in hippocampal neurons

  Di Wan, Tongchuang Lu, Chenyang Li, Changlong Hu, bioRxiv - Neuroscience 2023

  Quote: ... and anti-phospho-PKA antibody (1:1000, #5661, Cell Signaling Technology). The blots were developed using enhanced chemiluminescence reagents and imaged using either the ChemiDoc XRS+ imaging system from Bio-Rad (Hercules ...
• Human cerebrospinal fluid single exosomes in Parkinson’s and Alzheimer’s diseases

  Koya Yakabi, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD68 (D4B9C) (Cell Signaling Technology catalog #76437S), Amyloid Beta (6E10 ...
• PMP22 associates with MPZ via their transmembrane domains and disrupting this interaction causes a loss-of-function phenotype similar to hereditary neuropathy associated with liability to pressure palsies (HNPP)

  Natalya Pashkova, et al., bioRxiv - Neuroscience 2023

  Quote: ... mouse α-myc-Tag (9B11) monoclonal (2276, Cell Signaling Technology, Danvers, MA), rabbit α-mCherry polyclonal (AB356482 ...
• The LH receptor regulates hippocampal spatial memory and restores dendritic spine density in ovariectomized APP/PS1 AD mice

  Megan Mey, et al., bioRxiv - Neuroscience 2023

  Quote: ... Rac1 (1:1,000; Cell Signaling Technology, MA), TrkB (1:1,000 ...
• PMP22 associates with MPZ via their transmembrane domains and disrupting this interaction causes a loss-of-function phenotype similar to hereditary neuropathy associated with liability to pressure palsies (HNPP)

  Natalya Pashkova, et al., bioRxiv - Neuroscience 2023

  Quote: ... Peroxidase-conjugated (HRP) α-mouse (cat#:70776S) or α-rabbit secondary antibody (cat# 7074S) were obtained from Cell Signaling Technology. Dodecyl-β-D-maltoside (DDM ...
• The LH receptor regulates hippocampal spatial memory and restores dendritic spine density in ovariectomized APP/PS1 AD mice

  Megan Mey, et al., bioRxiv - Neuroscience 2023

  Quote: Hippocampal tissue was homogenized fresh after dissection using cell lysis buffer (Cell Signaling Technology, MA). Protein samples were separated using SDS-PAGE 10% precast gels (Bio-Rad ...
• De novo purine metabolism is a metabolic vulnerability of cancers with low p16 expression

  Naveen Kumar Tangudu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... HRP-linked (Cell Signaling Technology, 7074; 1:5000).
• Highly multiplexed, image-based pooled screens in primary cells and tissues with PerturbView

  Takamasa Kudo, et al., bioRxiv - Systems Biology 2023

  Quote: ... incubated with p65 antibody in PBS at 1:1000 (Cell Signaling Technologies #8242) for 1 hour and washed three times with PBST ...
• Highly multiplexed, image-based pooled screens in primary cells and tissues with PerturbView

  Takamasa Kudo, et al., bioRxiv - Systems Biology 2023

  Quote: ... with maleimide chemistry (Cell Signaling Technology #8478), Nos2 Alexa Fluor 532 conjugate (ThermoFisher Scientific ...
• Highly multiplexed, image-based pooled screens in primary cells and tissues with PerturbView

  Takamasa Kudo, et al., bioRxiv - Systems Biology 2023

  Quote: ... p65 Alexa Fluor 647 conjugate (Cell Signaling Technology #8801) and phospho-rpS6 Alexa Fluor 750 conjugate (Cell Signaling Technology #62788 ...