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• An AAV capsid reprogrammed to bind human Transferrin Receptor mediates brain-wide gene delivery

  Qin Huang, et al., bioRxiv - Neuroscience 2023

  Quote: ... RCAS1 (1:200 dilution, Cell Signaling Technology, 12290), and TGN46 (1:50 dilution ...
• The G₂ phase controls binary division of Toxoplasma gondii

  Lauren M. Hawkins, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit α-Myc (Cell Signaling), rabbit α-TgPCNA2 (kindly provided by Dr ...
• Highly multiplexed, image-based pooled screens in primary cells and tissues with PerturbView

  Takamasa Kudo, et al., bioRxiv - Systems Biology 2023

  Quote: ... and phospho-rpS6 Alexa Fluor 750 conjugate (Cell Signaling Technology #62788) in 3% BSA in PBS at 1:75 ...
• Comparative Interactome Analysis of α-arrestin Families in Human and Drosophila

  Kyung-Tae Lee, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following antibodies were used: TXNIP (14715, Cell Signaling Technology, Beverly, MA), HDAC2 (57156 ...
• Comparative Interactome Analysis of α-arrestin Families in Human and Drosophila

  Kyung-Tae Lee, et al., bioRxiv - Cell Biology 2023

  Quote: ... HDAC2 (57156, Cell Signaling Technology), H3ac antibody (39139 ...
• Age-dependent RGS5 loss in pericytes induces cardiac dysfunction and fibrosis in the heart

  Anita Tamiato, et al., bioRxiv - Physiology 2023

  Quote: Senescence staining was performed on paraffin sections using using the β-galactosidase staining kit (9860S, Cell Signaling) following the manufacturer’s instructions ...
• The role of subicular VIP-expressing interneurons on seizure dynamics in the intrahippocampal kainic acid model of temporal lobe epilepsy

  Sadegh Rahimi, et al., bioRxiv - Neuroscience 2023

  Quote: ... the free-floating coronal sections were incubated with 10% normal goat serum (catalog #5425, Cell Signaling) in Tris-HCl-buffered saline ...
• The role of subicular VIP-expressing interneurons on seizure dynamics in the intrahippocampal kainic acid model of temporal lobe epilepsy

  Sadegh Rahimi, et al., bioRxiv - Neuroscience 2023

  Quote: ... a secondary reaction was performed by simultaneously incubating the sections with a rabbit antibody directly conjugated to Alexa Fluor 488 (1:500; catalog #4412S, Cell Signaling) for CR and with a guinea pig antibody directly conjugated to Alexa Fluor 647 (1:500 ...
• Phosphatases of regenerating liver balance F-actin rearrangements during T cell activation through WD repeat containing protein 1

  Óscar Aguilar-Sopeña, et al., bioRxiv - Immunology 2023

  Quote: ... α-phospho-p44/p42 MAPK (ERK1/2) and α-total p44/p42 MAPK (ERK1/2) were from Cell Signaling (USA).
• Aberrant connective tissue sheath contraction drives premature hair follicle regression by inducing progenitor cell depletion in androgenetic alopecia

  Zhili Deng, et al., bioRxiv - Pathology 2023

  Quote: ... Rabbit anti-p-MLC2 (1:100, Cell Signaling), Rabbit anti-Ki67 (1:500 ...
• Aberrant connective tissue sheath contraction drives premature hair follicle regression by inducing progenitor cell depletion in androgenetic alopecia

  Zhili Deng, et al., bioRxiv - Pathology 2023

  Quote: ... The primary antibodies were used in this study: Rabbit anti-SOX9 (1:100, Cell Signaling), Mouse anti-K15 (1:2000 ...
• Fabry Cardiomyopathy: Myocardial Fibrosis, Inflammation and Down-Regulation of Mannose6Phosphate Receptors cause Low accessibility to Enzyme Replacement Therapy

  Andrea Frustaci, et al., bioRxiv - Pathology 2023

  Quote: ... (D8Z3J) polyclonal antibody (1:1000, Cell Signaling Technolo-gy). Anti-α-sarcomeric actin antibody (1:500 ...
• Aberrant connective tissue sheath contraction drives premature hair follicle regression by inducing progenitor cell depletion in androgenetic alopecia

  Zhili Deng, et al., bioRxiv - Pathology 2023

  Quote: ... Rabbit anti-CD200 (1:200, Cell Signaling), Rabbit anti-CD34 (1:250 ...
• Presenilin-dependent regulation of tau pathology via the autophagy/proteasome pathway

  Anna del Ser-Badia, et al., bioRxiv - Neuroscience 2023

  Quote: ... resolved on SDS-polyacrylamide gel electrophoresis and detected by Western blotting with the following antibodies: rabbit anti-tau (D1M9X; 1:1000, Cell Signaling), mouse anti-tau (TG5 ...
• CTCF/cohesin organize the ground state of chromatin-nuclear speckle association

  Ruofan Yu, et al., bioRxiv - Genetics 2023

  Quote: ... Anti-GAPDH antibody (1:1000, Cell Signaling, 5174); 4 ...
• CTCF/cohesin organize the ground state of chromatin-nuclear speckle association

  Ruofan Yu, et al., bioRxiv - Genetics 2023

  Quote: ... Anti-RAD21 antibody (1:1000, Cell Signaling, 4321s); 3 ...
• Genetically encoded tool for manipulation of ΔΨm identifies the latter as the driver of integrative stress response induced by ATP Synthase dysfunction

  Mangyu Choe, Denis V. Titov, bioRxiv - Biochemistry 2023

  Quote: ... rabbit anti-P-EIF2α (Cell Signaling Technologies, 3398S, 1:1000), mouse anti-EIF2α (Fisher Scientific ...
• Genetically encoded tool for manipulation of ΔΨm identifies the latter as the driver of integrative stress response induced by ATP Synthase dysfunction

  Mangyu Choe, Denis V. Titov, bioRxiv - Biochemistry 2023

  Quote: ... The primary antibodies used in this study were: rabbit anti-ATF4 (Cell Signaling Technologeis, 11815s, 1:625), rabbit anti-β-actin (Cell Signaling Technologies ...
• Genetically encoded tool for manipulation of ΔΨm identifies the latter as the driver of integrative stress response induced by ATP Synthase dysfunction

  Mangyu Choe, Denis V. Titov, bioRxiv - Biochemistry 2023

  Quote: ... rabbit anti-β-actin (Cell Signaling Technologies, 4970S, 1:2500), rabbit anti-P-EIF2α (Cell Signaling Technologies ...
• The expression of congenital Shoc2 variants induces AKT-dependent feedback activation of the ERK1/2 pathway

  Patricia Wilson, et al., bioRxiv - Biochemistry 2023

  Quote: ... phosphorylated – PAK1 (Thr423)/PAK2 (Thr402) (Cell Signaling, Danvers, MA, USA); total PAK1 and total AKT (Proteintech ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... AKT (1:1000; 9272; Cell Signaling Technology Inc.); Myosin ID (1:500 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... EEA1 (1:200; C45B10; 3288; Cell Signaling Technology Inc.); RAB7 (1:100 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... ERK1/2 (1:1000; 9102; Cell Signaling Technology, Inc.); phospho-AKT (Ser473 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... phospho-PDGFRα (Tyr 849)/PDGFRβ (Tyr857) (1:1000; C43E9; 3170; Cell Signaling Technology, Inc.); phospho-ERK1/2 (Thr202/Tyr204 ...
• Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila

  Katharina Eichler, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-HA (Cell Signaling Technology, Danvers ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... RAB11 (1:100; D4F5; 5589; Cell Signaling Technology Inc.) and Myosin ID (1:500 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following antibodies were used for western blotting: PDGFRα (1:1000; D13C6; 5241; Cell Signaling Technology, Inc., Danvers, MA, USA); PDGFRβ (1:1000 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... phospho-ERK1/2 (Thr202/Tyr204) (1:1000; 9101; Cell Signaling Technology, Inc.); ERK1/2 (1:1000 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... RAB7 (1:100; D95F2; 9367; Cell Signaling Technology Inc.); RAB4 (1:200 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... PDGFRβ (1:1000; 28E1; 3169; Cell Signaling Technology, Inc.); β-tubulin (1:1000 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... APPL1 (1:200; D83H4; 3858; Cell Signaling Technology Inc.); EEA1 (1:200 ...
• Mechanisms by which barrier-to-autointegration factor regulates dynamics of nucleocytoplasmic leakage and membrane repair following nuclear envelope rupture

  Charles T. Halfmann, et al., bioRxiv - Cell Biology 2023

  Quote: ... or rabbit anti-phospho-Lamin A/C (pSer22; 1:100; 2026S; Cell Signaling). Primary antibodies were detected using Alexa-Flour 488 conjugated goat anti-rabbit (1:1000 ...
• Lipid peroxidation does not mediate muscle atrophy induced by PSD deficiency

  Hiroaki Eshima, et al., bioRxiv - Physiology 2023

  Quote: ... incubated in appropriate secondary antibodies (Anti-rabbit IgG, 7074, Cell Signaling Technology; or anti-mouse IgG ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... Lamin B1 (1:1000; D4Q4Z, 12586; Cell Signaling Technology Inc.); phospho-PDGFRα (Tyr 849)/PDGFRβ (Tyr857 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... RAB5 (1:200; C8B1; 3547; Cell Signaling Technology Inc.); APPL1 (1:200 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following antibodies were used for immunofluorescence analysis: PDGFRα (1:1000; D13C6; 5241; Cell Signaling Technology, Inc.); PDGFRβ (1:1000 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... phospho-AKT (Ser473) (1:1000; 9271; Cell Signaling Technology Inc.); AKT (1:1000 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... PDGFRβ (1:1000; 28E1; 3169; Cell Signaling Technology, Inc.); Na+/K+-ATPase (1:500 ...
• Nuclear NFκB Activity Balances Purine Metabolism in Cellular Senescence

  Sho Tabata, et al., bioRxiv - Systems Biology 2023

  Quote: ChIP was carried out using the Simple ChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9005) following the manufacturer’s protocol ...
• Antidepressants stimulate lipoprotein(a) macropinocytosis via serotonin-enhanced cell surface binding

  Nikita Deo, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-LAMP1 (Cell Signaling Technologies) and rabbit anti-Rab11 (Cell Signaling Technologies) ...
• Targeting tumour-intrinsic neural vulnerabilities of glioblastoma

  Sohyon Lee, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Alexa Fluor® 555 anti-Cleaved Caspase-3 (1:500, Cell Signaling Technology, #9604S), Alexa Fluor® 546 anti-HOMER (1:300 ...
• Targeting tumour-intrinsic neural vulnerabilities of glioblastoma

  Sohyon Lee, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PE anti-CFOS (1:300, Cell Signaling Technology, #14609S, clone 9F6), FITC anti-ATF4 (1:300 ...
• Targeting tumour-intrinsic neural vulnerabilities of glioblastoma

  Sohyon Lee, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CHI3L1 (1:300, Cell Signaling Technology, #47066S, clone E2L1M), anti-Nestin (1:150 ...
• E-cadherin is a structuring component of invadopodia in pancreatic cancer

  Aurélie Dobric, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit anti-P-cadherin (21300S) and rabbit anti-Rab7 (D95F2) were from Cell Signaling. Mouse anti-Cortactin p80/p85 (4F11 ...
• Similar metabolic pathways are affected in both Congenital Myasthenic Syndrome-22 and Prader-Willi Syndrome

  Kritika Bhalla, et al., bioRxiv - Neuroscience 2023

  Quote: ... and anti-GAPDH (Cell Signaling Technology) antibodies ...
• Genetic and pharmacologic alterations of claudin9 levels suffice to induce functional and mature inner hair cells

  Yingying Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... and (13) rabbit anti-calbindin (Cell signaling technology, 1:200, Cat # 13176S) with appropriate secondary antibodies coupled to Alexa-405 ...
• Monocytes reprogrammed by 4-PBA potently contribute to the resolution of inflammation and atherosclerosis

  Shuo Geng, et al., bioRxiv - Immunology 2023

  Quote: ... or anti–β-actin antibody (1:1000 dilution, Cell Signaling Technology, no. 5125), followed by incubation with HRP-conjugated anti–rabbit IgG (1:1000 dilution ...
• Inflammation primes the kidney for recovery by activating AZIN1 A-to-I editing

  Segewkal Heruye, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Histone H3 (1B1B2, Cell Signaling, mouse monoclonal ...
• The Hao-Fountain syndrome protein USP7 regulates neuronal connectivity in the brain via a novel p53-independent ubiquitin signaling pathway

  Hao Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... immunolabeled with anti-c-cas 3 (Cell Signaling #9961) and anti-GFP antibody (Abcam #ab13970 ...
• Galectin-9 regulates dendritic cell contractility and migration via RhoA

  Guus Franken, et al., bioRxiv - Immunology 2023

  Quote: ... rabbit anti-Pak1 (#2602; Cell Signaling Technology) at 1:500 (v/v) ...