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• A Combination of Four Nuclear Targeted Effectors Protects Toxoplasma Against Interferon Gamma Driven Human Host Cell Death During Acute Infection

  Brittany Henry, L. David Sibley, Alex Rosenberg, bioRxiv - Microbiology 2023

  Quote: ... The supernatant was assayed by western blotting for acetylated histone H3K27 using anti-H3K27Ac antibody (Cell Signaling), whereas anti-H3 antibody (Cell Signaling ...
• Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

  Julio C.Y. Liu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... UBE2K (1:1,000, Cell Signaling Technology (3847, RRID:AB_2210768)) ...
• Aberrant connective tissue sheath contraction drives premature hair follicle regression by inducing progenitor cell depletion in androgenetic alopecia

  Zhili Deng, et al., bioRxiv - Pathology 2023

  Quote: ... Rabbit anti-p-MLC2 (1:100, Cell Signaling), Rabbit anti-Ki67 (1:500 ...
• Aberrant connective tissue sheath contraction drives premature hair follicle regression by inducing progenitor cell depletion in androgenetic alopecia

  Zhili Deng, et al., bioRxiv - Pathology 2023

  Quote: ... The primary antibodies were used in this study: Rabbit anti-SOX9 (1:100, Cell Signaling), Mouse anti-K15 (1:2000 ...
• Fabry Cardiomyopathy: Myocardial Fibrosis, Inflammation and Down-Regulation of Mannose6Phosphate Receptors cause Low accessibility to Enzyme Replacement Therapy

  Andrea Frustaci, et al., bioRxiv - Pathology 2023

  Quote: ... (D8Z3J) polyclonal antibody (1:1000, Cell Signaling Technolo-gy). Anti-α-sarcomeric actin antibody (1:500 ...
• Aberrant connective tissue sheath contraction drives premature hair follicle regression by inducing progenitor cell depletion in androgenetic alopecia

  Zhili Deng, et al., bioRxiv - Pathology 2023

  Quote: ... Rabbit anti-CD200 (1:200, Cell Signaling), Rabbit anti-CD34 (1:250 ...
• Dehydrozaluzanin C- derivative protects septic mice by alleviating over-activated inflammatory response and promoting the phagocytosis of macrophages

  Ying-xiang Zou, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... All the antibodies were bought from Cell Signaling Technology
• Endosomal-lysosomal organellar assembly (ELYSA) structures coordinate lysosomal degradation systems through mammalian oocyte-to-embryo transition

  Yuhkoh Satouh, et al., bioRxiv - Developmental Biology 2023

  Quote: The following primary antibodies were used: rabbit monoclonal anti-RAB5 (C8B1; 3547; Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-RAB7 (D95F2 ...
• Endosomal-lysosomal organellar assembly (ELYSA) structures coordinate lysosomal degradation systems through mammalian oocyte-to-embryo transition

  Yuhkoh Satouh, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit monoclonal anti-RAB7 (D95F2; 9367; Cell Signaling Technology), rat monoclonal anti-mouse LAMP1 (1D4B ...
• PNLDC1 catalysis and postnatal germline function are required for piRNA trimming, LINE1 silencing, and spermatogenesis in mice

  Chao Wei, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Testis sections were then incubated with anti-MIWI (1:100; 2079, Cell Signaling Technology), anti-MILI (1:100 ...
• Artemisinin resistance mutations in Pfcoronin impede hemoglobin uptake

  Imran Ullah, et al., bioRxiv - Cell Biology 2023

  Quote: ... Samples were then prepared using the PTMScan HS Ubiquitin Kit from Cell Signaling Technology (Cat. No. 59322). Following lysis ...
• A novel KCNJ16 kidney organoid model recapitulates the disease phenotype and shows restoration of lipid accumulation upon treatment with statins

  E. Sendino Garví, et al., bioRxiv - Cell Biology 2023

  Quote: ... organoids were mounted in Prolong gold containing DAPI or without DAPI (Cell Signaling Technology, Leiden, The Netherlands). Images were captured using the confocal microscope Leica TCS SP8 X (Leica Biosystems ...
• A novel KCNJ16 kidney organoid model recapitulates the disease phenotype and shows restoration of lipid accumulation upon treatment with statins

  E. Sendino Garví, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-GATA-3 (#5852S, Cell Signaling Technology, Leiden, The Netherlands) diluted 1:300 ...
• Nerve Growth Factor Receptor Limits Inflammation to Promote Remodeling and Repair of Osteoarthritic Joints

  Lan Zhao, Yumei Lai, Hongli Jiao, Jian Huang, bioRxiv - Cell Biology 2023

  Quote: ... 1/100 anti-p-SMAD1 (Cell Signaling, catalog # 9516S), 1/200 anti-SMAD1 (abcam ...
• Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidy

  Dorine C. Hintzen, et al., bioRxiv - Cell Biology 2023

  Quote: ... Membranes were probed with the following primary antibodies (1:1000): pan-RAS (Rabbit, Cell Signaling, #3965), HSP90 α/β (rabbit ...
• Nerve Growth Factor Receptor Limits Inflammation to Promote Remodeling and Repair of Osteoarthritic Joints

  Lan Zhao, Yumei Lai, Hongli Jiao, Jian Huang, bioRxiv - Cell Biology 2023

  Quote: ... 1/100 anti-p65 antibody (Cell Signaling, catalog #8242), 1/200 anti-RANKL (Novus Biologicals ...
• PINK1 and BNIP3 mitophagy inducers have an antagonistic effect on Rbf1-induced apoptosis in Drosophila

  Mélanie Fages, et al., bioRxiv - Cell Biology 2023

  Quote: ... First antibody incubation was made overnight using Dcp-1 antibody (Cell Signaling 95785S) diluted 1:100 in the same solution ...
• PINK1 and BNIP3 mitophagy inducers have an antagonistic effect on Rbf1-induced apoptosis in Drosophila

  Mélanie Fages, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary antibodies used: anti-HA (Cell Signaling, 3724S), Anti F1F0 (Abcam ...
• Reversible assembly and disassembly of V-ATPase during the lysosome regeneration cycle

  Ioana Sava, et al., bioRxiv - Cell Biology 2023

  Quote: ... Rabbit monoclonal antibody to RAB7a (#D95F2) was from Cell Signaling Technology. Mouse monoclonal antibodies to GAPDH (am4300 ...
• Reversible assembly and disassembly of V-ATPase during the lysosome regeneration cycle

  Ioana Sava, et al., bioRxiv - Cell Biology 2023

  Quote: ... phosphor-p70 S6 kinase (#9234) phospho-S6 ribosomal protein (#2217) from Cell Signaling Technology Inc ...
• Nerve Growth Factor Receptor Limits Inflammation to Promote Remodeling and Repair of Osteoarthritic Joints

  Lan Zhao, Yumei Lai, Hongli Jiao, Jian Huang, bioRxiv - Cell Biology 2023

  Quote: ... sections were treated with 1/100 anti-NGFR antibody (Cell Signaling, catalog # 8238S), 1/200 anti-RUNX2 antibody (MBL ...
• The Reelin Receptor ApoER2 is a Cargo for the Adaptor Protein Complex AP-4: Implications for Hereditary Spastic Paraplegia

  Mario O. Caracci, et al., bioRxiv - Cell Biology 2023

  Quote: ... and immunostained with anti-phospho-CREB Ser133 (Cell Signaling Technology, 87G3 Rabbit mAB #9198) and anti-MAP2 ...
• Comparison of gastric inflammation and metaplasia induced by Helicobacter pylori or Helicobacter felis colonization in mice

  Sara R. Druffner, et al., bioRxiv - Immunology 2023

  Quote: ... MIST1 (Cell Signaling Technologies), CD45 (clone 104 ...
• Porcine sapovirus protease controls the innate immune response and targets TBK1

  Iliana Georgana, et al., bioRxiv - Microbiology 2023

  Quote: ... and mCherry (Cell Signaling [CS-43590], rabbit monoclonal, 1:1,000). The PSaV VPg and NS6 antibodies were generated by immunisation of New Zealand White rabbits with purified recombinant VPg and NS6 ...
• A Combination of Four Nuclear Targeted Effectors Protects Toxoplasma Against Interferon Gamma Driven Human Host Cell Death During Acute Infection

  Brittany Henry, L. David Sibley, Alex Rosenberg, bioRxiv - Microbiology 2023

  Quote: ... whereas anti-H3 antibody (Cell Signaling) was used for the loading control.
• A Combination of Four Nuclear Targeted Effectors Protects Toxoplasma Against Interferon Gamma Driven Human Host Cell Death During Acute Infection

  Brittany Henry, L. David Sibley, Alex Rosenberg, bioRxiv - Microbiology 2023

  Quote: ... The supernatant was used to check the expression level of CBP and P300 by Western blot using anti-P300 and anti-CBP antibodies (Cell Signaling). Anti–β-tubulin antibody (Hybridoma bank # AB_2315513 ...
• Porcine sapovirus protease controls the innate immune response and targets TBK1

  Iliana Georgana, et al., bioRxiv - Microbiology 2023

  Quote: ... Membranes were blocked in 0.1% Tween PBS supplemented with 5% (w/v) skimmed milk and subjected to immunoblotting with the following primary antibodies at the indicated dilutions: TBK1 (Cell Signaling [CS-3013], rabbit polyclonal, 1:500); GAPDH (Invitrogen [AM4300] ...
• Kit Ligand and Kit receptor tyrosine kinase sustain synaptic inhibition of Purkinje Cells

  Tariq Zaman, et al., bioRxiv - Neuroscience 2023

  Quote: ... α-GAPDH (1:2000, Clone 14C10, product 2118 Cell Signaling Technology), or α-parvalbumin antibody (1:3000 ...
• Kit Ligand and Kit receptor tyrosine kinase sustain synaptic inhibition of Purkinje Cells

  Tariq Zaman, et al., bioRxiv - Neuroscience 2023

  Quote: ... Western blots were probed with Rabbit: α-Kit (1:1000, clone D13A2, Cell Signaling Technologies product 3074), α-GAPDH (1:2000 ...
• N-6-methyladenosine (m6A) Promotes the Nuclear Retention of mRNAs with Intact 5’ Splice Site Motifs

  Eliza S. Lee, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and HRP-conjugated secondaries (Cell Signaling). All antibodies were diluted 1:1000 to 1:2000 for western blotting.
• Dominant and Genome-Wide Formation of DNA:RNA Hybrid G-Quadruplexes in Living Yeast Cells

  Chen-xia Ren, et al., bioRxiv - Biochemistry 2023

  Quote: ... one half incubated with 20 µl mouse IgG anti-FLAG M2 magnetic beads for G4 pull-down and the other half incubated with 20 µl mouse IgG magnetic beads (Cat#5873; Cell Signaling Technology) for non-specific control ...
• Overburdened Ferroptotic Stress Impairs Tooth Morphogenesis

  H.S. Wang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... cleaved Caspase 3 (1:200, Cell Signaling Technology, 9664) in blocking buffer (PBS and 2% bovine serum albumin ...
• Cannabinoid Combination Targets NOTCH1-Mutated T-ALL Through the Integrated Stress Response Pathway

  Elazar Besser, et al., bioRxiv - Cancer Biology 2023

  Quote: ... freshly supplemented with protease and phosphate inhibitors (Cell Signaling Technology; 5872). Lysates were cleared by centrifugation at 13,000 g at 4°C for 10 min ...
• Maternal, placental and fetal response to a non-viral, polymeric nanoparticle gene therapy in nonhuman primates

  Rebecca L. Wilson, et al., bioRxiv - Physiology 2023

  Quote: ... The membranes were then washed and further incubated with a HRP conjugated secondary (Cell Signaling 7074S 1:1000) for 1.5 h at room temperature ...
• Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition

  Dylan C. Sarver, et al., bioRxiv - Physiology 2023

  Quote: ... HRP-conjugated goat anti-rabbit (CST#7074) and goal anti-mouse (CST#7076) antibodies were from Cell Signaling Technology.
• Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition

  Dylan C. Sarver, et al., bioRxiv - Physiology 2023

  Quote: ... Rabbit monoclonal antibody against phospho-JAK2 (Tyr1008)(D4A8) were obtained from Cell Signaling Technology (CST # 8082). Rabbit polyclonal antibody against phospho-STAT5 (Y694 ...
• Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition

  Dylan C. Sarver, et al., bioRxiv - Physiology 2023

  Quote: ... Rabbit polyclonal antibody against phospho-STAT5 (Y694) was obtained from Cell Signaling Technology (CST#9351). Mouse monoclonal anti-FLAG M2 antibody (F1804 ...
• Cytoskeletal adaptation following long-term dysregulation of actomyosin in neuronal processes

  Bruno A. Cisterna, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-GAPDH (1/1,000, cat#2118S, Cell Signaling). For secondary antibodies ...
• Effect of gedunin on glioblastoma proliferation and invasiveness: In vitro and in vivo approaches

  Thadeu Estevam Moreira Maramaldo Costa, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... rabbit, pan, 40D4), phospho-AKT [rabbit, Ser473, (D9E)XP] and GAPDH (glyceraldehyde-3-phosphate dehydrogenasemouse, D4C6R) were purchased from Cell Signaling (USA). Antibodies against human PCNA (proliferating cell nuclear antigen ...
• Acylation of MLKL impacts its function in necroptosis

  Apoorva J. Pradhan, et al., bioRxiv - Biochemistry 2023

  Quote: Antibodies were obtained from Cell Signaling Technology (rabbit monoclonal anti-MLKL ...
• Astrocytes gate long-term potentiation in hippocampal interneurons

  Weida Shen, et al., bioRxiv - Neuroscience 2023

  Quote: ... The following antibodies were used: mouse monoclonal anti-GFAP (1/1000, Cell Signaling Technology Cat #3670, RRID: AB_561049), mouse monoclonal anti-NeuN (1/500 ...
• Astrocytes gate long-term potentiation in hippocampal interneurons

  Weida Shen, et al., bioRxiv - Neuroscience 2023

  Quote: ... the sections were then incubated with the following secondary antibody: Alexa Fluor 594 goat anti-mouse (1/1000, Cell Signaling Technology Cat# 8890, RRID: AB_2714182) at room temperature for 2 h ...
• E3 ubiquitin ligase Deltex facilitates the expansion of Wingless gradient and antagonizes Wingless signaling through a conserved mechanism of transcriptional effector Armadillo/β-catenin degradation

  Vartika Sharma, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-β-catenin (1:1000, Cell Signaling), Rabbit anti-GAPDH (1:1000 ...
• E3 ubiquitin ligase Deltex facilitates the expansion of Wingless gradient and antagonizes Wingless signaling through a conserved mechanism of transcriptional effector Armadillo/β-catenin degradation

  Vartika Sharma, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Rabbit anti-GAPDH (1:1000, Cell Signaling). Images were obtained with Chemidoc XRS+ (Biorad).
• Claudin-2 limits pancreatitis development through regulating tight junction-controlled pancreatic ductal transport

  Sneha Kesaraju, et al., bioRxiv - Pathology 2023

  Quote: ... formalin fixed paraffin embedded tissue sections were stained with rabbit-anti claudin-2 (1:100, Cell Signaling Technology Cat# 28530, RRID: AB_2798961), and rat-anti CD45 (1:100 ...
• Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome

  Kira Cozzolino, et al., bioRxiv - Biochemistry 2023

  Quote: Antibodies used are as follows: anti-STAT1 (RRID: AB_2198300; Cell Signaling Technology, 9172), anti-phospho STAT1 S727 (RRID ...
• Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome

  Kira Cozzolino, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-phospho STAT1 S727 (RRID: AB_2197983; Cell Signaling Techology, 9177), and anti-GAPDH (RRID ...
• The genetic and dietary landscape of the muscle insulin signalling network

  Julian van Gerwen, et al., bioRxiv - Systems Biology 2023

  Quote: ... Insulin-stimulated cells were additionally incubated in primary antibodies against pT308 Akt (Cell Signaling Technologies #2965; diluted 1:1000), pS473 Akt (Cell Signaling Technologies #9271 ...
• The genetic and dietary landscape of the muscle insulin signalling network

  Julian van Gerwen, et al., bioRxiv - Systems Biology 2023

  Quote: ... pT246 PRAS40 (Cell Signaling Technologies #13175; diluted 1:1000), total PRAS40 (Cell Signaling Technologies #2691 ...
• The genetic and dietary landscape of the muscle insulin signalling network

  Julian van Gerwen, et al., bioRxiv - Systems Biology 2023

  Quote: ... total PRAS40 (Cell Signaling Technologies #2691 ...