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• Remodeling of the protein ubiquitylation landscape in the aging vertebrate brain

  Antonio Marino, et al., bioRxiv - Systems Biology 2023

  Quote: ... the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) kit (Cell Signaling Technology, 59322) was used following manufacturer instructions ...
• Inhibition of the macrophage demethylase LSD1 reverses Leishmania amazonensis-induced transcriptomic changes and causes a decrease in parasite load

  Maria Gutiérrez-Sanchez, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary antibody solution (α-LSD1 antibody, Cell Signaling, 1/100 ...
• Myospreader improves gene editing in skeletal muscle by myonuclear propagation

  Kiril K. Poukalov, et al., bioRxiv - Cell Biology 2023

  Quote: ... Gapdh (Cell Signaling Technology, 2118L) (abcam ...
• Myospreader improves gene editing in skeletal muscle by myonuclear propagation

  Kiril K. Poukalov, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary antibodies used were against HA-tag (Cell Signaling Technology 3724S), tdTomato (Takara Bio ...
• The glucocorticoid dexamethasone inhibits HIF-1alpha stabilisation and metabolic reprogramming in lipopolysaccharide-stimulated primary macrophages

  Sally A Clayton, et al., bioRxiv - Immunology 2023

  Quote: ... HRP-conjugated secondary antibodies were obtained from Cell Signaling Technologies (#7074, #7076) and applied for 1hour at room temperature ...
• Early antigen receptor signaling in natural killer cells alters STAT4-dependent fate decisions via epigenetic remodeling

  Simon Grassmann, et al., bioRxiv - Immunology 2023

  Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20uM EDTA + 4uM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification50,51.
• Multimodal single cell-resolved spatial proteomics reveals pancreatic tumor heterogeneity

  Yanfen Xu, et al., bioRxiv - Systems Biology 2023

  Quote: ... and anti-CD11b (Cell Signaling Technology, clone E6E1M, dilution 1:1000) were used for staining KPf/fC mouse tumor sections ...
• AMP-activated protein kinase is necessary for Treg cell functional adaptation to microenvironmental stress

  Manuel A. Torres Acosta, et al., bioRxiv - Immunology 2023

  Quote: ... samples were processed with Immunofluorescence Application Solutions Kit (Cell Signaling cat. no. 12727) following the manufacturer’s protocol ...
• AMP-activated protein kinase is necessary for Treg cell functional adaptation to microenvironmental stress

  Manuel A. Torres Acosta, et al., bioRxiv - Immunology 2023

  Quote: ... Processed samples were then blotted with antibodies against DNMT1 (Cell Signaling cat. no. 5032), AMPKα1 (Abcam cat ...
• AMP-activated protein kinase is necessary for Treg cell functional adaptation to microenvironmental stress

  Manuel A. Torres Acosta, et al., bioRxiv - Immunology 2023

  Quote: ... or isotype control (Cell Signaling cat. no. 7074) overnight at 4 °C with constant agitation ...
• AMP-activated protein kinase is necessary for Treg cell functional adaptation to microenvironmental stress

  Manuel A. Torres Acosta, et al., bioRxiv - Immunology 2023

  Quote: ... 1:50 anti-AMPKα (Cell Signaling cat. no. 2532S), and 1:50 anti-β-actin (Abcam cat ...
• Astrocytes regulate neuronal network burst frequency through NMDA receptors species- and donor-specifically

  Noora Räsänen, et al., bioRxiv - Neuroscience 2023

  Quote: ... PSD95 rabbit (1:500, Cell Signaling Technologies, 3450), GRIN1 rabbit (1:500 ...
• Functional diversity of NLRP3 gain-of-function mutants associated with CAPS autoinflammation

  Camille Cosson, et al., bioRxiv - Immunology 2023

  Quote: ... anti-IL-1β (D3U3E, Cell Signaling), anti-IL-18 (D2F3B ...
• Activation of Protein Kinase R (PKR) Plays a Pro-Viral Role in Mammarenavirus Infected Cells

  Haydar Witwit, et al., bioRxiv - Microbiology 2023

  Quote: ... rabbit mAb anti-peIF2α (Ser51) (D9G8) XP® (3398T, Cell Signaling); Phospho- eIF2α-S51 mouse mAb (AP0692 ...
• Activation of Protein Kinase R (PKR) Plays a Pro-Viral Role in Mammarenavirus Infected Cells

  Haydar Witwit, et al., bioRxiv - Microbiology 2023

  Quote: ... rabbit mAb anti-PKR (D7F7) (12297S, Cell signaling technology) 1 in 1000 ...
• Generation of a porcine cell line stably expressing pig TMPRSS2 for efficient isolation of viruses from pigs with respiratory diseases

  Yuri L Tanaka, et al., bioRxiv - Microbiology 2023

  Quote: ... TMPRSS2 expression was evaluated using SimpleWestern Abby (ProteinSimple, San Jose, CA, USA) with an anti-Myc tag mouse monoclonal antibody (Cell Signaling Biotechnology ...
• Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophages

  Charul Jani, et al., bioRxiv - Microbiology 2023

  Quote: ... GAPDH (Cell Signaling Technology 2118S), p62 (Sigma P0067) ...
• Peptidoglycan-Chi3l1 interaction shapes gut microbiota in intestinal mucus layer

  Yan Chen, Ruizhi Yang, Bin Qi, Zhao Shan, bioRxiv - Microbiology 2023

  Quote: ... The secondary antibodies used were goat anti-Rat-IgG (Cell Signaling, 7077s, 1:1000) and goat anti-mouse (Invitrogen ...
• Host autophagy is exploited by the intracellular parasite Toxoplasma gondii to enhance amino acids levels

  Matthew D. White, et al., bioRxiv - Microbiology 2023

  Quote: ... Immunoblot analyses were done using primary antibodies (Cell signaling: FAM13B ...
• IFIH1 (MDA5) is required for innate immune detection of intron-containing RNA expressed from the HIV-1 provirus

  Mehmet Hakan Guney, et al., bioRxiv - Microbiology 2023

  Quote: ... rabbit anti-DDX58/RIG-I (1:1000 dilution; Cell Signaling Technology, #3743S), rabbit anti-MAVS (1:1000 dilution ...
• Innate extracellular Hsp70 inflammatory properties are mediated by the interaction of Siglec-E and LOX-1 receptors

  Thiago J. Borges, et al., bioRxiv - Immunology 2023

  Quote: ... SHP-1 (clone E1U6R, 1:50) and rabbit IgG isotype control from Cell Signaling. Cells were analyzed using FACS Canto II or Fortessa X-20 (both from BD Biosciences) ...
• E3 ligase ASB3 downregulates antiviral innate immunity by targeting MAVS for ubiquitin-proteasomal degradation

  Mingyang Cheng, et al., bioRxiv - Immunology 2023

  Quote: ... anti-ubiquitin (K48) (8081) and HRP-conjugated mouse anti-rabbit IgG (Conformation Specific) (5127) antibodies (Cell Signaling Technology); anti-ubiquitin (WT ...
• Bone marrow-derived CCR5-expressing macrophages promote venous thrombus resolution by up-regulating plasminogen activators and VEGF

  Mizuho Nosaka, et al., bioRxiv - Molecular Biology 2023

  Quote: ... rabbit anti-mouse GAPDH pAbs (Cell Signaling Technology, Danvers, MA).
• HELZ promotes R loop resolution to facilitate DNA double-strand break repair by homologous recombination

  Ramona Haji-Seyed-Javadi, et al., bioRxiv - Molecular Biology 2023

  Quote: ... RPA70 (Cell Signaling # 2267S; 1:120 for IF); CtIP (Millipore # MABE1060 ...
• Restoring AIBP expression in the retina provides neuroprotection in glaucoma

  Won-Kyu Ju, et al., bioRxiv - Neuroscience 2023

  Quote: ... Membrane were washed three times with T-TBS then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... eIF2α (#9722, 1:1,000; Cell Signaling) and β–actin (#A2228 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... 4E-BP1/2 (#2845, 1:1,1000; Cell Signaling), phospho-mTOR Ser2448 (#2971 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... p70S6K1 (#9206, 1:1,1000; Cell Signaling), phospho-S6 Ser235/236 (#4858 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-ERK1/2 Thr202/Tyr204 (#4370, 1:1,000; Cell Signaling), ERK1/2 (#9102 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... ERK1/2 (#9102, 1:1,000; Cell Signaling); phospho-p70S6K1 Thr389 (#9205 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... S6 (#2217, 1:1,000; Cell Signaling), phospho-4E-BP1 Ser65 (#9451 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-S6 Ser240/244 (#5364, 1:1,000; Cell Signaling), S6 (#2217 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-4E-BP1 Ser65 (#9451, 1:1,000; Cell Signaling), 4E-BP1/2 (#2845 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... mTOR (#2983, clone 7C10, 1:1,1000; Cell Signaling), phospho-eIF2α Ser51 (#3597 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-S6 Ser235/236 (#4858, 1:1,000; Cell Signaling), phospho-S6 Ser240/244 (#5364 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-p70S6K1 Thr389 (#9205, 1:1,000; Cell Signaling), p70S6K1 (#9206 ...
• The ketamine metabolite (2R,6R)-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

  Felipe C. Ribeiro, et al., bioRxiv - Neuroscience 2023

  Quote: ... phospho-mTOR Ser2448 (#2971, 1:1,1000; Cell Signaling), mTOR (#2983 ...
• Integrating multiple omics levels using the human protein complexome as a framework, a multi-omics study of inborn errors of metabolism

  Mainak Guharoy, et al., bioRxiv - Biochemistry 2023

  Quote: ... Anti-mouse (Cell Signaling, Cat# 7076) and anti-rabbit (Cell Signaling ...
• Dehydrozaluzanin C- derivative protects septic mice by alleviating over-activated inflammatory response and promoting the phagocytosis of macrophages

  Ying-xiang Zou, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... All the antibodies were bought from Cell Signaling Technology
• Age, Sex and Alzheimer’s disease: A longitudinal study of 3xTg-AD mice reveals sex-specific disease trajectories and inflammatory responses mirrored in postmortem brains from Alzheimer’s patients

  Alicia J. Barber, et al., bioRxiv - Neuroscience 2023

  Quote: ... We obtained CD45 rabbit monoclonal antibody (D3F8Q, catalog #70257) and F4/80 XP rabbit monoclonal antibody (D2S9R, catalog #70076 from Cell Signaling Technology.
• Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in Drosophila

  Shivan L. Bonanno, et al., bioRxiv - Neuroscience 2023

  Quote: HA was labeled with 1:500 rabbit anti-HA (Cell Signaling Technology, Cat#3724 ...
• Fluoxetine treatment during postnatal and juvenile temporal epochs evokes diametrically opposing changes in anxio-depressive behaviors, gene expression, mitochondrial function, and neuronal architecture in the medial prefrontal cortex

  Utkarsha Ghai, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit phosphorylated p-70 S6K (1:250, Cell Signaling Technologies), rabbit actin (1:5000 ...
• Fluoxetine treatment during postnatal and juvenile temporal epochs evokes diametrically opposing changes in anxio-depressive behaviors, gene expression, mitochondrial function, and neuronal architecture in the medial prefrontal cortex

  Utkarsha Ghai, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit p-70 S6K (1:1000, Cell Signaling Technologies), rabbit phosphorylated p-70 S6K (1:250 ...
• Fluoxetine treatment during postnatal and juvenile temporal epochs evokes diametrically opposing changes in anxio-depressive behaviors, gene expression, mitochondrial function, and neuronal architecture in the medial prefrontal cortex

  Utkarsha Ghai, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit p-ULK1 (1:1000, Cell Signaling Technologies), rabbit p-70 S6K (1:1000 ...
• Fluoxetine treatment during postnatal and juvenile temporal epochs evokes diametrically opposing changes in anxio-depressive behaviors, gene expression, mitochondrial function, and neuronal architecture in the medial prefrontal cortex

  Utkarsha Ghai, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit ULK-1 (1:1000, Cell Signaling Technologies), rabbit p-ULK1 (1:1000 ...
• Fluoxetine treatment during postnatal and juvenile temporal epochs evokes diametrically opposing changes in anxio-depressive behaviors, gene expression, mitochondrial function, and neuronal architecture in the medial prefrontal cortex

  Utkarsha Ghai, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit p-mTOR (1:1000, Cell Signaling Technologies), rabbit ULK-1 (1:1000 ...
• Fluoxetine treatment during postnatal and juvenile temporal epochs evokes diametrically opposing changes in anxio-depressive behaviors, gene expression, mitochondrial function, and neuronal architecture in the medial prefrontal cortex

  Utkarsha Ghai, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit 4EBP (1:1000, Cell Signaling Technologies), rabbit p-4EBP1 (1:500 ...
• Small-molecule degraders reduce Aβ production through CAPRIN1-mediated lysosomal degradation of APP in Alzheimer’s iPSC-derived neurons

  Sunghan Jung, et al., bioRxiv - Neuroscience 2023

  Quote: ... ADAM10 (Cell Signaling Technology, 14194), Nicastrin (Cell Signaling Technology ...
• Small-molecule degraders reduce Aβ production through CAPRIN1-mediated lysosomal degradation of APP in Alzheimer’s iPSC-derived neurons

  Sunghan Jung, et al., bioRxiv - Neuroscience 2023

  Quote: ... Presenilin 2 (Cell Signaling Technology, 9979), BACE1 (Abcam ...
• Small-molecule degraders reduce Aβ production through CAPRIN1-mediated lysosomal degradation of APP in Alzheimer’s iPSC-derived neurons

  Sunghan Jung, et al., bioRxiv - Neuroscience 2023

  Quote: ... Nicastrin (Cell Signaling Technology, 5665), GGA1 (Proteintech ...