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Citations for Jackson ImmunoResearch :
401 - 437 of 437 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 hour at room temperature (RT) in a blocking buffer containing 10% normal donkey serum (NDS) (cat. 017-000-001, Jackson ImmunoResearch, West Grove, USA), 1% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were blocked for 1 hour with block buffer (0.05% IGEPAL CA-630, 0.05% Triton X-100, 5% goat normal serum [Jackson ImmunoResearch; 005-000-121] in 1xPBS). Samples were incubated with primaries/nanobodies diluted in block buffer for 16 hours at 4 °C on a rocker ...
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bioRxiv - Systems Biology 2024Quote: ... Slides were washed once with PBS and blocked for one hour at room temperature in blocking buffer of PBS with 1% Normal Donkey Serum (Jackson ImmunoResearch #017-000-121), 0.1% Gelatin ...
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bioRxiv - Immunology 2024Quote: ... After washing twice with 200 μl FACS buffer the cells were incubated in 30 μl of FACS buffer for 20 min with 1:1000 diluted APC-conjugated F(ab’)₂ fragment goat anti-human IgG (Jackson ImmunoResearch, West Grove, USA). Following two further washes with FACS buffer the cells were resuspended in 100 μl FACS buffer with 0.1 µg/ml propidium iodide (BD Pharmingen) ...
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bioRxiv - Immunology 2024Quote: ... slides/wells were washed in 0.01-M PBS and incubated in the same blocking buffer but with appropriate secondary antibodies (647 donkey anti-guinea pig, and 594 donkey anti-rabbit, Jackson Immunoresearch, West Grove, PA). The gelatin-embedded DRG sections in the wells were then placed carefully on gelatin-coated slides ...
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bioRxiv - Neuroscience 2024Quote: ... blocked tissues overnight with blocking buffer and stained with a polyclonal goat anti-mouse F(ab’)2 fragment (1:100; Jackson ImmunoResearch 115-005-072) and rabbit anti-mouse NF200 (1:100 ...
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bioRxiv - Genetics 2024Quote: ... Sections were rinsed in PBS and then blocked for 1 hour at room temperature in Blocking Buffer consisting of 10% normal donkey serum (Jackson ImmunoResearch, 017-000-121), 0.5% Triton X-100 (Sigma Aldrich ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... yeast were washed in PBS/0.2% BSA and rotated at 18 rpm in 300 μL of same buffer containing 2 μg/mL Alexa488-conjugated goat anti-chicken IgG (Jackson ImmunoResearch, West Grove, PA) for one hour at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were washed with 1X DPBS and then incubated in blocking buffer (consisting of 5% (v/v) normal donkey serum (Jackson ImmunoResearch, West Grove, PA) and 1% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... DNA-conjugated secondary antibody staining was performed by incubating the sample with a donkey anti-mouse secondary fragment antibody (Jackson ImmunoResearch, 715-007-003) at a 1:100 dilution in 5% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Li-Cor) (for bulk striatal samples analysis and eIF5A1/eIF5A2 antibody characterization) or AffiniPure Goat anti-rabbit IgG (H+L) (Jackson ImmunoResearch, 111-035-003) for the rest of WBs ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X for 10 min each in T-Buffer followed by one wash in TBS and incubation in Alexa Fluor 647-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch, diluted at 1:250) and Alexa Fluor 488-conjugated donkey anti-rat IgG (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2020Quote: ... and secondary antibodies diluted in blocking buffer were applied for 1 hour at RT (TRITC for α-SMA; Cy5 for fibronectin, Jackson ImmunoResearch, West Grove, PA). Next ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed three times with diluted blocking buffer (15 min each) before incubation with secondary antibodies (Ch-647: Jackson Immunoresearch, Cat# 703-605-155) for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed three times with TBS buffer with 0,05 % Tween-20 and further incubated with anti-rabbit Alexa 647 (Jackson ImmunoResearch Cat. No. 111-606-144), anti-human IgG-Alexa647 (Southern Biotech ...
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bioRxiv - Genetics 2020Quote: ... Sections were washed twice with PBS for 10 minutes then blocked for 1 hour at room temperature in permeabilization/blocking buffer comprised of PBS with 10% normal goat serum (Jackson ImmunoResearch, Cat. 005-000-121), 1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... live cells expressing S delta 19aa were suspended in QSol Buffer (IntelliCyte Corporation) to which a cocktail of Fc-specific secondary antibodies was added: AF647 goat anti-human IgG (Jackson ImmunoResearch, Cat # 109-605-008), AF488 goat anti-human IgA (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 µL of FACS buffer containing secondary R-PE Goat Anti-Human IgG antibody diluted 1:100 (Jackson ImmunoResearch, cat #109-117-008), and incubated for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... We then washed cells three times in binding buffer and incubated them with a R-phycoerythrin (PE)-coupled goat anti-human F(ab’)2 fragment (Jackson ImmunoResearch Cat #: 109-116-098) in binding buffer for 30 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes and washed 3 times in PBS for 5 minutes at room temperature (RT), followed by incubation for 30 minutes in blocking buffer (1% IgG-free BSA, 4% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were rinsed three times with 1xPBS and permeabilized with room temperature permeabilization buffer (0.3% IGEPAL CA-630 [Sigma-Aldrich; I8896], 0.05% Triton X-100 [Sigma-Aldrich; T8787], 0.1% BSA [Jackson ImmunoResearch; 001-000-162] in 1xPBS) for 3 minutes followed by another three washes with 1xPBS ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 7 days with secondary antibodies (goat anti-rabbit Alexa-555, 1:200, A27039, Thermo Fisher; donkey anti-guinea pig, Alexa-647, 1:400, 706-605-148, Jackson ImmunoResearch; in antibody buffer) at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS the sample was incubated for 3-4h with a secondary donkey anti-rabbit antibody (711-005-152; Jackson ImmunoResearch, West Grove, PA, USA) that was conjugated to Alexa Fluor 647-NHS at an average degree of labeling of 1.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were rinsed with TBST buffer three times and then incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) in a blocking solution at RT for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... and further incubated in phosphate buffer saline containing anti-HRP (conj. FITC) and a secondary TRITC-conjugated anti-mouse IgG antibody (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), both at 1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed four times in PBS for 15 min and placed in blocking buffer containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) and 0.1% Triton X-100 in PBS for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... and blocked with 100 μL blocking buffer (1% bovine serum albumin and 5% normal donkey serum [Jackson ImmunoResearch, cat 017-000-121, lot 152961] in PBST) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 hour at room temperature (RT) in blocking buffer containing 10% normal donkey serum (NDS) (Jackson ImmunoResearch, West Grove, USA, #017-000-001), 1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated with secondary antibodies (Alexa fluor 647 donkey-anti-chicken and Alexa fluor donkey-anti-rabbit, Jackson ImmunoResearch, 1:1000 dilution in blocking buffer). Once stained ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated for 20 min on ice with FITC-conjugated rabbit anti-hamster IgG antibody (1:500 in FACS-buffer; 307-095-003, Jackson ImmunoResearch Labs, West Grove, Pennsylvania, USA). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated in DyLight 649 or 488 conjugated Hrp at 1:500 (#123-605-021, #123-485-021; Jackson ImmunoResearch Laboratories, West Grove, PA, USA). For Phalloidin staining ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 4X for 5 min each in T-Buffer (TBS containing 0.05% Triton-X100) followed by incubation in Alexa Fluor 647-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA, diluted at 1:200) for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Cancer Biology 2020Quote: ... domes were washed 3x with IF Buffer and counterstained with Alexa Fluor 488-AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) (Jackson ImmunoResearch, Cat: 703-545-155, 1:500), Donkey anti-Mouse IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... 3x for 5 min and incubated at room temperature with blocking buffer containing goat anti-rabbit HRP secondary antibody (Jackson Immunoresearch labs, #111-035-003, 1:5000). Blots were rinsed in wash buffer 3x for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed in Wash Buffer three times for 10 minutes each and incubated with FITC-conjugated anti-mouse (1:200; Jackson ImmunoResearch Laboratories, Inc; Cat# 115-095-062) and TRITC-conjugated anti-rabbit (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed in Wash Buffer three times for 10 minutes each and then incubated with FITC-conjugated anti-mouse (1:200; Jackson ImmunoResearch Laboratories, Inc; Cat# 115-095-062) and/or TRITC-conjugated anti-rabbit (1:200 ...