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Citations for Jackson ImmunoResearch :
151 - 200 of 437 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and resuspended in FACS staining buffer [PBS with 0.2% IgG-free bovine serum albumin (Jackson ImmunoResearch), and 0.1% NaN3 (Sigma Aldrich)] ...
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bioRxiv - Molecular Biology 2023Quote: ... were washed in cell lysis buffer and blocked in 10% BSA (Jackson ImmunoResearch 001-000-162) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 3% NGS in Tris-A buffer for CD88 (C5aR1) and CD68/IBA1 markers (NGS, Jackson ImmunoResearch Cat ...
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bioRxiv - Bioengineering 2024Quote: ... permeabilized with 0.3% Triton buffer and blocked with 5% goat serum (Jackson ImmunoResearch 005-000-001) prior to being tagged with a primary antibody. ...
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bioRxiv - Neuroscience 2024Quote: ... and wash/resuspension buffer (PBS containing 0.5% BSA (Bovine Serum Albumin, Jackson ImmunoResearch #001-000-162) was added to the pellet ...
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bioRxiv - Biophysics 2024Quote: ... The permeabilized cells were incubated in blocking buffer (1xPBS containing 3% (wt/vol) BSA (Jackson ImmunoResearch)) for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... then incubated during 1h at room temperature with 1/10,000 dilution of the corresponding peroxidase-conjugated secondary antibodies in blocking solution: donkey anti-rabbit and anti-mouse from Jackson Immunoresearch. Blots were finally washed in TBST and analyzed using Super Signal West Dura or Femto (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in a blocking solution composed of PBST with 3% normal donkey serum (Jackson ImmunoResearch, #017-000-121) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated in a secondary antibody solution at room temperature for 2 h with protection from light (1:500, Alexa Fluor 647 donkey anti-chicken, Jackson Immunoresearch, USA ...
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bioRxiv - Neuroscience 2021Quote: The brain sections undergoing immunohistochemical staining were washed in PBS 3 times (10 minutes each time) and incubated in a blocking solution containing 0.3% TritonX-100 and 5% normal donkey serum (Jackson ImmunoResearch, USA) in PBS for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed with PBS three times for 20 minutes and then incubated with commercial dye-conjugated secondary antibodies diluted in blocking solution (AF647-conjugates from Jackson ImmunoResearch Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... sections were rinsed three times in 1X PBS and incubated in secondary antibody solution Cy-3 donkey anti-rabbit (1:500; Jackson ImmunoResearch) or Cy-3 donkey anti-mouse (1:500 ...
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bioRxiv - Genetics 2020Quote: ... Brains were then incubated in a block solution containing a donkey anti-mouse secondary antibody conjugated with Cy5 (1:400 dilution, Jackson ImmunoResearch) and a rabbit anti-GFP antibody conjugated with Alexa Fluor 488 (1:500 dilution ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 1 hour at 37°C with blocking solution of 5% goat serum in PBS (catalog no. 005-000-121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (catalog no ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated for 2 hr at room temperature with corresponding Alexa-conjugated secondary antibodies diluted to 1:800 in blocking solution (goat anti-rabbit Alexa 488 and goat anti-rat Alexa 594 antibodies, Jackson ImmunoResearch). Sections were washed ...
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bioRxiv - Biochemistry 2020Quote: ... the grids were put on drops of milk solution containing (1:100) goat anti-rabbit IgG coated conjugated with 18 nm colloidal gold particles (Jackson ImmunoResearch). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... Selected slices were then blocked for 3 h at room temperature in a PBS solution containing 5% normal donkey serum (NDS; Jackson ImmunoResearch) and 1% of non-ionic detergent (Triton® X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... brain slices were incubated with the following secondary antibodies in the blocking solution at 37°C overnight: donkey anti-goat-rhodamine RX (RRX) (705-297-003; 1:300; Jackson ImmunoResearch), donkey anti-rabbit-AlexaFluor 488 (711-545-152 ...
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bioRxiv - Neuroscience 2020Quote: ... brain slices were incubated with the following secondary antibodies in the blocking solution at RT 3 hours: donkey anti-mouse-AlexaFluor 488 (715-545-150; 1:300; Jackson ImmunoResearch), donkey anti-G.P.-RRX (706-295-148 ...
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bioRxiv - Molecular Biology 2021Quote: ... was diluted in blocking solution and detected by a secondary anti-guinea pig Cy3-antibody (Jackson ImmunoResearch Laboratories Inc., 1:800) that was also diluted in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then rinsed three times in 1X TBS and incubated in secondary antibody solution (Alexa 647 donkey anti-rabbit IgG, 1:500 (Jackson Immunoresearch); Alexa 488 donkey anti rat IgG ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated for 1hr at 37°C with blocking solution of 5% goat serum in PBS (catalog no. 005–000-121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (catalog no ...
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Geraniol Enhances Inhibitory Inputs to Paraventricular Thalamic Nucleus and Induces Sedation in MicebioRxiv - Neuroscience 2021Quote: The brain sections undergoing immunohistochemical staining were washed in PBS for 3 times (10 minutes each time) and incubated in a blocking solution containing 0.3% TritonX-100 and 5% normal donkey serum (Jackson ImmunoResearch, USA) in PBS for 1 hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 1 h at RT with blocking solution of 5% goat serum in PBS (catalog no. 005–000-121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (catalog no ...
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bioRxiv - Neuroscience 2020Quote: ... Several rinsing with TBS was followed by incubation in secondary antibody solution for 2 hours (1:500 Cy3 conjugated goat anti-rabbit IgG, #134845, Jackson ImmunoResearch).
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bioRxiv - Neuroscience 2020Quote: ... GFP was amplified with an anti-chicken GFP antibody from Abcam and retina slices were incubated overnight with primary antibodies in blocking solution followed by washes in PBS and a 3-hour incubation with anti-isotypic secondary antibodies (Invitrogen or Jackson Immunoresearch). Labeling of Dact2-GFP or Plcxd2-GFP retinas for GAD67 (mouse monoclonal ...
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bioRxiv - Neuroscience 2020Quote: ... the brains were washed three times at room temperature in 0.25% PBST and incubated for 3 days at 4°C in a secondary antibody solution consisting of goat anti-mouse Cy3 (Jackson ImmunoResearch 115-165-062 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed in 0.1M PBS and 0.1% TritonX-100 in PBS before incubation with blocking solution (5% donkey serum (Jackson ImmunoResearch, Pennsylvania, US) in 0.05% Triton-X100 in 0.1M PBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS multiple times and placed in a fluorophore conjugated secondary antibody solution for 30 minutes on ice (1:500, 109-546-097, Jackson ImmunoResearch). Cells were collected and washed before being placed in PBS and passed through a cell strainer ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated for 1 hour at room temperature with blocking solution of 5% goat serum in PBS (catalog no. 005–000–121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (catalog no ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 3 h in the blocking solution with the addition of 16 µg/ml of donkey anti-guinea pig Alexa Fluor 647 (Jackson ImmunoResearch). Following two more 30-min PBS rinses ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated for 1-2 hrat room temperature with blocking solution of 5% goat serum in PBS (005–000-121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GTX135357 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with PBS three times and then incubated in blocking solution (PBS supplemented with 0.1% Triton-X-100 [X100, Sigma-Aldrich] and 10% normal donkey serum [017-000-121, Jackson ImmunoResearch]) for at least one hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 × 5 min in PBS at room temperature and incubated in secondary antibody solution (Dk anti Rb AF 488, 1:500, Jackson ImmunoResearch; Gt anti Ms AF 633 ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.3% PBT were followed by 30 min incubation in blocking solution with 5% NGS (005-000-121, Jackson Immunoresearch Laboratories) in PBT ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated for 90 minutes in secondary 1:1000 biotin solution (anti-mouse IgG1, Jackson ImmunoResearch; West Grove, PA, USA), followed by a 60-minute incubation with secondary 1:500 streptavidin (SA-HRP ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBST (0.1% Tween-20 in PBS) and then incubated in secondary antibody solution consisting of donkey anti-goat-488 (IgG H+L) (Jackson ImmunoResearch, cat ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBST and then incubated in secondary antibody solution consisting of donkey anti-mouse-594 (IgG H+L) (Jackson ImmunoResearch, cat ...
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bioRxiv - Neuroscience 2023Quote: ... then blocked for 45 min before 2 h incubation at room temperature in Alexa Fluor 488-conjugated donkey anti-chicken IgG (1:400 in blocking solution, Jackson ImmunoResearch). Sections were rinsed in PBS after the final incubation and mounted with Vectashield Hardset Mounting Medium with DAPI (Vector Labs) ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated for 90 min in secondary biotin solution (anti-mouse IgG1, 1:1000, Jackson ImmunoResearch; West Grove, PA, USA), followed by a 60-min incubation with a secondary cocktail containing AF647 anti-mouse IgG2b (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... All sections were rinsed three times for 15 min in PBS-T and incubated for 4 hours at ∼20 °C in secondary antibodies in the blocking solution (Cy3 donkey anti-guinea pig, 1:400, Jackson ImmunoResearch, catalog no ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS and immediately transferred to the secondary antibody solution: Cy3 goat anti-mouse (1:500, Jackson ImmunoResearch) for 2 hours at home temperature ...
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bioRxiv - Neuroscience 2024Quote: ... we incubated sections for 2 hours in a secondary antibody solution with biotinylated donkey anti-rabbit (1:500; #711-065-152 Jackson ImmunoResearch) in PBT-NDS-azide ...
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Foxn3 is part of a transcriptional network that regulates cilia genes in the developing mouse retinabioRxiv - Developmental Biology 2024Quote: Retina sections were treated with a blocking solution (1% unlabeled adinity-purified Fab fragment donkey anti-mouse IgG (H+L) and 5% donkey serum (Jackson Immunoresearch) in PBS containing 0.1% Triton X-100 (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were then washed and probed with 1:200 FITC-conjugated anti-HRP (Jackson ImmunoResearch) and Alexa Fluor 568-conjugated anti-mouse secondary antibodies (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2020Quote: ... samples were incubated with biotin-SP-conjugated rabbit anti-chicken secondary antibody (1:250, Jackson ImmunoResearch). Samples were washed and incubated in Vectastain ABC reagent (Vector laboratories) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... samples were incubated with biotin-SP-conjugated rabbit anti-chicken secondary antibody (1:250, Jackson ImmunoResearch). Samples were washed and incubated in Vectastain ABC reagent (Vector laboratories) ...
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bioRxiv - Physiology 2023Quote: ... Samples were blocked for at least 3 hours in 5% normal goat serum (NGS; Jackson ImmunoResearch) and 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were washed in PBS and incubated with anti-mouse Alexa 488 (1:500, Jackson ImmunoResearch) and anti-rabbit Cy3 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated with goat anti-mouse-488 (1:250) (Jackson ImmunoResearch, 115-545-146) O/N at 4°C ...