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Citations for Jackson ImmunoResearch :
301 - 350 of 437 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Neurons were then incubated in blocking buffer containing 5% bovine serium albumin (BSA) and 5% Normal Goat Serum (Jackson ImmunoResearch) for 30min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS followed by 1 h incubation in blocking buffer [PBS complemented with 0.5% BSA (IgG-Free, Protease-Free, #001-000-162, Jackson ImmunoResearch), 0.02% Triton X-100 and 1:100 diluted donkey serum] at RT ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA, USA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 1 hour at room temperature with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch: catalog no. 005–000-121). To stain for SARS-CoV-2 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in fresh PBS and kept in the secondary antibodies solution (donkey anti-goat Alexa Fluor 467 and donkey anti-mouse Cy3, 1:300; Jackson ImmunoResearch, West Grove, PA, USA) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated for 5 minutes in DyLight 488 conjugated Hrp (2 µL of stock solution applied directly to filleted larvae and washed twice with HL3.1 before imaging) (#123-485-021; Jackson ImmunoResearch Laboratories, West Grove, PA, USA). Live imaging was performed as described above.
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bioRxiv - Cell Biology 2023Quote: ... Cover slips were then blocked in 150 μL of blocking solution (5% [w/v] BSA, 5% [v/v] Normal Goat Serum [Jackson ImmunoResearch Laboratories] in 1× PBS) at RT for 90 min ...
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bioRxiv - Genomics 2024Quote: ... Blocking solution (10% normal goat serum in PBS and/or AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch laboratories, 115-007-003) was applied for 30 minutes at RT followed by primary antibodies [perilipin (Cell signaling ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Animals were then placed in a secondary antibody solution of Alexa Flour 488 AffiniPure Goat Anti-Rabbit IgG (1:500, Jackson ImmunoResearch, cat. 111-545-144) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed twice for 10 minutes at room temperature with TBS and incubated for 90 minutes at room temperature with solution containing secondary antibodies (all at 1:500 dilutions; Cy3 donkey anti-goat (Jackson Immunoresearch laboratories #705-165-003); Cy3 donkey anti-rabbit (Jackson Immunoresearch laboratories # 711-165-152) ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were incubated for 1 hour with gold-conjugated secondary antibodies (Jackson ImmunoResearch, #115-205-166 and 111-195-144). Sections were stained with 2% uranyl acetate and observed under a FEI electron microscope at 80 kV ...
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bioRxiv - Biophysics 2023Quote: ... Both samples were then labeled with an oligonucleotide-conjugated goat anti-rabbit IgG secondary antibody (115-005-146; Jackson ImmunoResearch) diluted 1:200 in block buffer ...
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bioRxiv - Immunology 2023Quote: ... Sample binding was detected via goat-anti-mouse IgG Alexa Fluor 647 conjugated polyclonal antibody (Jackson ImmunoResearch, 115-605-071). The secondary antibody was diluted in secondary binding buffer (1x TBS ...
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bioRxiv - Bioengineering 2023Quote: ... we then incubated the four samples with an equal volume of 71.5 ng/ml of AF647 goat anti-mouse IgG (Jackson ImmunoResearch), which binds to the oligo-barcoded Anti-VEGF-A antibody which is mouse species ...
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bioRxiv - Neuroscience 2024Quote: ... rinsed the samples 3x in PBS and processed with secondary antibody (Anti-Chicken IgY, 488, 703-545-155, Jackson ImmunoResearch). Tissue sections were rinsed again 3x in PBS ...
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bioRxiv - Immunology 2024Quote: ... These samples were then blocked for one hour with 1% normal donkey serum (017--000--121, Jackson ImmunoResearch, RRID: AB_2337258) and 0.1% Triton-X in phosphate buffer solution (PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated with AffiniPure Fab fragment goat anti-mouse IgG (1:40, catalog number: 115-007-003, Jackson ImmunoResearch) solution in 0.2 µm filtered PBS at room temperature for 30 mins ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then permeabilized in IF buffer (PBS supplemented with 0.1% Triton X-100, 5% normal donkey serum (017-000-121; Jackson ImmunoResearch Laboratories), and 3% bovine serum albumin (BP1605-100 ...
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bioRxiv - Neuroscience 2021Quote: ... tissue is washed 10 times in 1X PBS for 1 min each and then incubated for 2 hours in blocking buffer + secondary antibodies from Jackson ImmunoResearch Laboratories (donkey anti-rabbit 488 ...
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bioRxiv - Neuroscience 2020Quote: ... The brains then washed twice (10 mins) in 0.5% PBST buffer and blocked overnight in 10% Normal Goat Serum (NGS; Jackson ImmunoResearch Lab) at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then washed three times for 15 minutes in TBST buffer followed by incubation with either secondary donkey anti-rabbit-HRP antibody (711-035-152, Jackson ImmunoResearch) or goat anti-mouse-HRP antibody (1010-05 ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated in blocking buffer (PBS 0.5 % triton X-100 with 5 % normal goat serum and 5 % normal horse serum (Jackson Immunoresearch Laboratories)) for 2 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... blots were incubated for 1 hour at room temperature with horseradish peroxidase-conjugated secondary antibodies in TBST buffer as follows: 1:20,000 donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories) for SUR1 ...
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bioRxiv - Microbiology 2022Quote: ... bacterial pellets were re-suspended in 50 μl blocking buffer (PBS containing 1% (w/v) BSA and 20% Normal Mouse Serum (Jackson ImmunoResearch), incubated for 20 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... in 500 μL of RIPA buffer were incubated overnight at 4°C with unspecific mouse/rabbit/rat IgG antibodies (Jackson Immunoresearch) or rat anti-HA-tag (Roche Diagnostics ...
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bioRxiv - Neuroscience 2020Quote: ... donkey anti-mouse-Alexa488 conjugated was used as secondary antibody diluted in GDB buffer for 1 hour at room temperature (1:200, Jackson ImmunoResearch). Slides were mounted using mowiol-DABCO mounting medium.
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bioRxiv - Developmental Biology 2022Quote: ... with blocking buffer (1% bovine serum albumin and 5% normal donkey serum [Jackson ImmunoResearch, #017-000-121, lot #152961] in PBST). The blocking buffer was removed and replaced with a solution of primary antibodies in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted 1:300 in blocking buffer and with the Cy3 AffiniPure F(ab’)₂ Fragment Donkey Anti-Rat IgG (H+L) antibody (#712-165-513, Jackson ImmunoResearch) diluted 1:150 in blocking buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Antibody staining and slide blocking were performed in 1X antibody dilution buffer (ADB) (normal donkey serum (Jackson ImmunoResearch, West Grove, PA), 1X PBS ...
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bioRxiv - Immunology 2021Quote: ... cells (1 × 106) were washed with staining buffer and incubated with whole rat and mouse IgG (Jackson ImmunoResearch, West Grove, PA) for 30 min on ice to block Fc receptors ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and then incubated for 1 hr in blocking buffer without NaN3 to which of horseradish peroxidase (HRP)-conjugated secondary antibody goat anti-human IgG Fc specific antibody (Jackson Immunoresearch) had been added at the dilution as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... buffer to remove nonspecific antibodies and then incubated with horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse IgG (Jackson ImmunoResearch) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then washed again 3 times for 5 minutes each in 0.1M PBS before being incubated in blocking buffer with 1:1000 Alexa Fluor® 594 Streptavidin (Jackson Immunoresearch) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The brain slices were subsequently incubated in blocking buffer (5% donkey serum in TBS-T) containing appropriate fluorescence-conjugated secondary antibodies (1:1000; Jackson ImmunoResearch; Dylight 405-conjugated donkey anti-guinea pig IgG [706-475-148] ...
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bioRxiv - Bioengineering 2022Quote: ... Fixed samples were permeabilized and blocked overnight at 4 °C with a blocking buffer containing 5% IgG-free bovine serum albumin (BSA) (Jackson ImmunoResearch) and 0.3% Triton X-100 (Bio Basic ...
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bioRxiv - Cell Biology 2023Quote: ... + 10% FBS first and then with IF Buffer + 10% FBS + 20 µg/ml goat anti-Rabblit Fc fragment (111-005-046, Jackson ImmunoResearch). Acini were incubated with the primary antibodies in the secondary block solution washed with IF buffer and then incubated with secondary antibody in IF Buffer +10% FBS ...
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bioRxiv - Physiology 2023Quote: ... samples were incubated with secondary antibodies diluted 1:500 and DAPI (1:1000) in blocking/permeabilisation buffer (Dk-a-Ch-AF488 – 703-545-155 Jackson Immunoresearch, Dk-a-Rb-AF546 – A10040 Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed three times for 10 minutes in 0.1M Tris-buffered saline (TBS) and then incubated in 0.1M TBS with 5% blocking buffer (Normal Donkey Serum, Jackson Immunoresearch, NC9624464) and 0.3% Triton-X 100 for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated with 0.3% Triton-X in PBS (wash buffer) for 30 minutes for permeabilization and then with 5% Normal goat serum (Jackson ImmunoResearch) in wash buffer (blocking buffer) ...
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bioRxiv - Biochemistry 2023Quote: ... Triton X-100 in PBS at room temperature for 15 min and washed with the blocking buffer [5% (v/v) normal goat serum (NGS, Jackson ImmunoResearch) and 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... sections were blocked for 1 hour at room temperature in blocking buffer: 10% normal donkey serum (Jackson ImmunoResearch, 017-000-121) in PBST (0.1% TritonX-100 in PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were dissected and then washed twice (10 min) in 0.5% PBST buffer before being blocked overnight in 10% Normal Goat Serum (NGS; Jackson ImmunoResearch Lab) at 4 C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed in PBS and then blocked for 1 hour in Blocking Buffer consisting of 10% normal donkey serum (017-000-121, Jackson ImmunoResearch), 0.5% Triton X-100 (T8787 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 2-hour incubation in buffer containing biotinylated donkey anti-rabbit IgG (1:250, Jackson ImmunoResearch, 711-065-152), then washed again ...
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bioRxiv - Genomics 2024Quote: ... and then incubated with primary antibodies in A+t buffer at 4°C overnight and then with fluorescently-labeled secondary antibodies (1:200 dilution, Jackson ImmunoResearch). Testes were stained with 0.5 μg/mL DAPI/PBS and mounted in 80% glycerol on slides ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed three times for 20 min each in PBST and incubated for 3 days at 4°C in a secondary antibody solution consisting of goat anti-mouse Cy3 (Jackson ImmunoResearch, AB_2338680, 1:200 v/v) and goat anti-rabbit Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 h at room temperature in a solution containing a mixture of goat-Cy3 anti-rabbit (1:500, Jackson ImmunoResearch Laboratories, Cat. #111–165–144), goat anti-chicken Alexa 647 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were washed twice with filtered PBS and then resuspended in 0.2 µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG (Jackson ImmunoResearch, 1:100, 109-605-098, AB_2337889) and Brilliant Violet 421-anti-rabbit IgG (BioLegend ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Anti-RFamide antibody (1:1,000 in the blocking solution) generated based on identification by mass spectrometry analyses50 and Alexa488-conjugated anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used as the primary and secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were rinsed with PBS and incubated in a solution of PBS-diluted 10% normal goat serum or normal donkey serum (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), containing 1% bovine serum albumin (BSA ...