Labshake search
Citations for Jackson ImmunoResearch :
251 - 300 of 437 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... we incubated samples with donkey-anti-mouse Ab fused to HRP (1:5,000 Jackson ImmunoResearch 715-036-151). Pierce ECL Western Blotting Substrate was used for HRP detection ...
-
bioRxiv - Developmental Biology 2023Quote: ... The samples were then immunized by secondary antibodies coupled to AlexaFluor 647 (1/500, Jackson Immunoresearch, lot #146920) and or Cy3 (1/500 ...
-
bioRxiv - Cell Biology 2022Quote: ... and blocked for 45 min in block buffer (0.05 % NP40, 0.05 % Triton-X 100 and 5 % goat serum (Jackson ImmunoResearch) in PBS) ...
-
bioRxiv - Neuroscience 2022Quote: ... slides were incubated for one hour in blocking buffer containing 5% normal donkey serum (Jackson ImmunoResearch, West Grove, PA) and 0.3% Triton X-100 in PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... Slides were incubated for 30 minutes in blocking buffer containing 10% normal donkey serum (Jackson ImmunoResearch, West Grove, PA) and 1% bovine serum albumin (BSA ...
-
bioRxiv - Neuroscience 2024Quote: ... Blocking buffer consisted of 5% heat-shocked BSA in Tris-buffered saline and 10% normal donkey serum (Jackson Immunoresearch). Immunofluorescence buffer was comprised of 2% heat-shocked BSA in Tris-buffered saline ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were resuspended in DMEM with 10% FBS and spun at 500Xg for 5 min after which the cells were incubated in permeabilization buffer (1% BSA, 0.1% saponin in PBS, 10% normal donkey serum (Cat.# AB_2337258, Jackson ImmunoResearch) for 30 min at RT and thereafter labeled with tyrosine hydroxylase antibody or isotype controls (1:400 ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were blocked for 1 h in blocking buffer (0.05% NP40, 0.05% Triton-X 100 and 5% goat serum (Jackson ImmunoResearch) in PBS ...
-
bioRxiv - Immunology 2024Quote: ... Detection antibodies conjugated to HRP were diluted in blocking buffer: rabbit anti-mouse IgM (Jackson Immunoresearch, 315-035-049) diluted 1:5000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... re-suspended in FACS buffer and incubated with secondary goat anti-mouse antibody conjugated to allophycocyanin (APC; Jackson ImmunoResearch). Cells were then washed ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were blocked and permeabilized with in a buffer containing 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121), 1% non-fat milk ...
-
bioRxiv - Cell Biology 2022Quote: ... Mouse tissue sections incubated with mouse monoclonal antibodies were first incubated with a solution containing donkey anti-mouse IgG AffiniPure Fab fragments (1:25 in PBS; Jackson ImmunoResearch #715-007-003) for one hour prior to blocking ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 45-60 min in blocking solution that consisted of PBS containing 3% Bovine Serum Albumin (Jackson Immunoresearch, 001-000-162) and 3% Donkey Serum (Jackson ImmunoResearch ...
-
bioRxiv - Developmental Biology 2021Quote: ... The chips were then washed twice with PBS, and the cells were permeabilized with blocking solution (PBS 0.1% Tryton-X, 3% of Donkey Serum (Jackson Immunoresearch Cat#017-000-121)) for 45 minutes at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... for 20min at room temperature and then incubated in a blocking solution made of PBST with 10% normal donkey serum (Jackson ImmunoResearch, 017-000-121) for 30 min ...
-
bioRxiv - Neuroscience 2020Quote: ... tissue sections were washed and placed into Alexa 488-conjugated donkey anti-rabbit immunoglobulin G (IgG; 1:500 in blocking solution; Jackson Immunoresearch, West Grove, PA) for 30 minutes ...
-
Serotonin Modulates an Inhibitory Input to the Central Amygdala from the Ventral Periaqueductal GraybioRxiv - Neuroscience 2022Quote: ... and then incubated in a secondary antibody solution made with donkey anti-chicken IgY conjugated to Alexa-Fluor 488 (Jackson Immunoresearch, West Grove, PA) diluted 1:200 in PBS for 2 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were incubated in a PBS solution containing the biotinylated secondary antibody (Biotinylated goat anti-mouse, #115-065-166, Jackson Immunoresearch, diluted 1/200) for 1 hour at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... Larval brains were washed twice with 0.5% PBS-T and incubated for 1-7 days at 4°C in a blocking solution of 1% goat serum (Jackson ImmunoResearch, West Grove, PA), 1% donkey serum (Jackson ImmunoResearch ...
-
bioRxiv - Neuroscience 2022Quote: ... then treated for 2 hours in a PBS solution containing 0.3% TritonX-100 and 10% normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, West Grove, PA). Sections were then treated overnight at 4°C with a PBS solution containing 1% NDS ...
-
bioRxiv - Neuroscience 2023Quote: ... The slides were washed 3x for 30 min in PBS and incubated in a secondary antibody solution containing 1:500 dilution of anti-rabbit Cy3-conjugated (Jackson ImmunoResearch, 111-165-144) and anti-mouse Cy-5 conjugated (Jackson ImmunoResearch ...
-
bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3x with PBS and incubated for 1 h at RT in a secondary antibody solution containing 1:500 dilution of anti-rabbit Cy3-conjugated (Jackson ImmunoResearch, 111-165-144) and anti-mouse Cy-5 conjugated (Jackson ImmunoResearch ...
-
bioRxiv - Neuroscience 2022Quote: ... the sections were first washed in PBS 5mins for 3 times and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA), 1% bovine serum albumen (A2153 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mouse tissue sections to be incubated with mouse monoclonal antibodies were first incubated with a solution containing donkey anti-mouse IgG AffiniPure Fab fragments (1:25 in PBS; Jackson ImmunoResearch #715-007-003) for one hour prior to blocking ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1 ...
-
bioRxiv - Neuroscience 2023Quote: ... They were then incubated overnight at room temperature with a secondary antibody solution consisting of 12-nm Colloidal Gold Affini Pure Goat Anti-Rabbit IgG (Jackson ImmunoResearch, 111-205-144) at a 1:30 dilution in the blocking solution ...
-
bioRxiv - Developmental Biology 2023Quote: ... Tissue was dissected to isolate the cochlea and vestibular organs and these were permeabilized for 30 minutes in blocking solution containing PBS with 1% bovine serum albumin (BSA) (Jackson ImmunoResearch 000-001-162) and 5% donkey serum (Jackson ImmunoResearch 017-000-121) ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + 0.03% Triton + primary antibody (rabbit anti-P2RY12 ...
-
bioRxiv - Neuroscience 2024Quote: ... washed 3 times in PB 0.1 M, a secondary antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, goat anti-rabbit Cyanine Cy3TM (Jackson Immunoresearch, 111-165-003, RRID: AB_2338000), goat anti-mouse Cy5TM (Jackson Immunoresearch ...
-
bioRxiv - Immunology 2021Quote: ... HA-specific IgG Abs from the samples were detected using HRP- conjugated anti-human IgG secondary Abs (Jackson ImmunoResearch) and were developed using ABTS with acid stop ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were incubated in a 1:50 dilution of donkey anti-rabbit immunogold conjugate (Jackson ImmunoResearch, 711-205-152) in 5% BSA / PBS (20 min ...
-
bioRxiv - Immunology 2020Quote: ... Samples were harvested and unconjugated AffiniPure Fab Fragment Goat anti-human IgG (H+L) (Jackson Immunoresearch; #109-007-003) and Human TruStain FcX block (BioLegend ...
-
bioRxiv - Immunology 2021Quote: ... following AutoSTOMP2.0 cross linking some samples were washed and stained with AlexaFluor-594 Streptavidin (#016-580-084, Jackson ImmunoResearch) in TBST ...
-
bioRxiv - Cell Biology 2021Quote: ... samples were incubated with 1:1000 with anti-rabbit alkaline phosphatase antibody (1:1000, Jackson immunoresearch, Baltimore, PA, USA) and stained using Fast Red substrate kit according the manufacture instructions (Abcam ...
-
bioRxiv - Cancer Biology 2021Quote: ... samples were blocked with donkey anti-mouse IgG Fab fragments (20 μg/ml: Jackson Immunoresearch: Cat #715-007-003) as previously described (26) ...
-
bioRxiv - Neuroscience 2023Quote: ... The samples were blocked at room temperature for 40 min in PBT containing 2.5% normal goat serum (Jackson ImmunoResearch) and 2.5% normal donkey serum (Jackson ImmunoResearch) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Blocked samples were then incubated at 4°C overnight in primary antibody (rabbit anti-beta-galactosidase, Jackson ImmunoResearch Laboratories) dissolved at a 1:200 dilution in 1% BSA in PBS/0.1% Triton-X 100 ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were washed with PBS-Tx0.1% and incubated with secondary antibodies Donkey anti-Chicken biotinylated (Jackson ImmunoResearch, 1:500) and Donkey anti-mouse 647 (Jackson ImmunoResearch ...
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were washed in PBST and blocked with 2% normal donkey serum (Jackson ImmunoResearch Laboratories, Inc. 017-000-121) in PBST ...
-
bioRxiv - Immunology 2021Quote: ... blots were washed three times for ten minutes each in TBST at RT and then rocked for 1hr at RT in blocking buffer containing secondary antibodies (1:10,000; Jackson ImmunoResearch): anti-rat-horseradish-peroxidase (HRP ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then blocked for 1 hour in PFN buffer (PBS without Ca2+ and Mg2+ and containing 10% FBS and 0.02% azide) with 2% normal goat serum (Jackson ImmunoResearch). Cells were incubated for 1 hour with the appropriate primary antibodies diluted in blocking medium ...
-
bioRxiv - Immunology 2021Quote: ... 50 µl secondary antibody diluted in buffer A (PE anti-human IgG (1:200 dilution; Jackson ImmunoResearch #109-116-098)) or PE anti-human IgM (1:80 dilution ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated in blocking buffer containing PBST (0.01% Triton X-100 in PBS, pH 7.4) and 10% normal donkey serum (Jackson Immunoresearch labs) for 30 minutes at RT ...
-
bioRxiv - Neuroscience 2020Quote: ... The primary antibody against RFP was detected with a 2-hour incubation in diluted donkey-anti-rabbit-IgG-Cy3 antibody in blocking buffer (1:1,000, Jackson ImmunoResearch). Finally ...
-
bioRxiv - Neuroscience 2020Quote: ... were detected with a 2- hour incubation in adequate secondary antibodies diluted in blocking buffer (1:1,000 for donkey-anti-mouse-IgG-Cy5 or donkey-anti-rabbit-IgG-Cy3, Jackson ImmunoResearch). Finally ...
-
bioRxiv - Immunology 2021Quote: ... cells were washed with ice-cold wash buffer and stained with 1.5 ug/ml Alexa Fluor647-labeled Goat Anti-Human IgG secondary Ab (Jackson Immunoresearch) for 30 min on ice in the dark ...
-
bioRxiv - Cell Biology 2021Quote: ... Kidney were incubated in blocking buffer consisting of PTxwH with 3% normal donkey serum (Jackson Immunoresearch, West Grove, PA, USA) at 37°C for 48 hours ...
-
bioRxiv - Immunology 2022Quote: ... cells were washed with ice-cold wash buffer and stained with 1.5 µg/ml Alexa Fluor647-labeled Goat Anti-Human IgG secondary Ab (Jackson Immunoresearch) for 30 min on ice in the dark ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed twice with Perm buffer and incubated with Cy3-conjugated anti- mouse antibody (1:800, Jackson ImmunoResearch Labs) for 30 minutes on ice ...
-
bioRxiv - Cancer Biology 2023Quote: ... and incubated with blocking buffer (5% normal donkey serum [Jackson ImmunoResearch] in 0.1% Triton X-100 in PBS [PBS-Tx]) for 1 hour at RT ...