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Citations for Jackson ImmunoResearch :
351 - 400 of 437 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... incubated 2 h in blocking solution containing rabbit-anti-human amyloid beta (IBL America, #18584; 1:500) and donkey-anti-human AF647 (Jackson ImmunoResearch, 709-606-149; 1:250), washed 3x for 15 min each in PBS with 0.3% triton X-100 ...
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bioRxiv - Biophysics 2020Quote: ... the sample was washed with PBS (with calcium) and incubated with 1 mg/ml Fc fragment (Jackson ImmunoResearch, West Grove, PA) for 1 hour ...
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Programmed ER fragmentation drives selective ER inheritance and degradation in budding yeast meiosisbioRxiv - Cell Biology 2021Quote: ... Samples were then washed 3x in PBS-BSA and incubated with preabsorbed FITC-conjugated secondary antibody (Jackson ImmunoResearch Labs, 6:200) for 1 hr at RT ...
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bioRxiv - Neuroscience 2022Quote: ... samples were then incubated with a secondary antibody: donkey anti-rabbit secondary antibody conjugated with Cy3 (Jackson ImmunoResearch Labs, RRID:AB 2307443). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed and incubated with a fluorescently-labeled secondary antibodies (1:200 goat anti-rabbit Alexa Fluor 488, Jackson Immunoresearch or 1:1000 goat anti-mouse IgG1 Alexa Fluor 555 ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... Samples were rinsed three times with PBS and blocked for 15 min using PBS with 5% goat serum (Jackson ImmunoResearch Laboratories) and 0.5% Triton X-100 (Tx ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was washed 3x with 0.1M PBS and then incubated with a solution containing 1:500 Biotin-SP-conjugated Donkey anti-rabbit secondary antibody (Jackson ImmunoResearch Labs Cat# 711-065-152, RRID:AB_2340593). After washing ...
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bioRxiv - Neuroscience 2020Quote: ... selected coronal sections were rinsed three times in TBS and incubated in blocking solution (3% normal donkey serum, Jackson Immunoresearch AB2337258; 0.3% Triton X-100 in TBS) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary gold labeling was performed for 1.5 hours at 1:20 dilution in blocking solution (18 nm gold donkey-anti-mouse and donkey-anti-rabbit IgG, Jackson ImmunoResearch 705-215-147, 711-215-152). Post-staining ...
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bioRxiv - Neuroscience 2023Quote: ... The diaphragms were then washed and secondary antibody solution was added for 1 hour at room temperature: FITC anti-mouse IgG secondary (Jackson ImmunoResearch Laboratories, West Grove, PA; 1:100). Diaphragms were mounted with Vectashield mounting medium (Vector Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... Lung lobes were then transferred to secondary antibody solution (1:50 Alexa Fluor 790 AffiniPure Fab Fragment Goat anti-rabbit, Jackson Immunoresearch Laboratories, Cat no: 111-657-008), in blocking buffer and incubated for a further 2 days at 37°C with gentle shaking for in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... the membranes were incubated with a horseradish peroxidase-conjugated secondary antibody (goat polyclonal HRP Anti-mouse, code 115-035-003, Jackson ImmunoResearch, diluted 1:10.000 in blocking solution) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... After the brain slices were washed 3x with 1XPBS they were incubated with the following secondary antibodies in blocking buffer for 2 hours at room temperature: 1:500 dilution of Donkey anti-rat Alexa Fluor 488 (Jackson ImmunoResearch Laboratories), 1:200 dilution of Donkey anti-guinea pig Alexa Fluor 647 (VWR) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then transferred to fresh blocking buffer with diluted antibodies (RyR2 1:500, NCX 1:200, SERCA 1:2000, GAPDH 1:5000) (Jackson ImmunoResearch, PA) followed by overnight incubation on a shaker at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then washed twice with wash buffer and incubated with an anti-rabbit Alexa Fluor 488–conjugated secondary antibody (Jackson ImmunoResearch Laboratories) in antibody buffer for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... The fixed cells were washed with PBS then resuspended in FACS staining buffer (DPBS [Dulbecco’s Phosphate-Buffered Saline] (Cellgro, Manassas, VA) with 0.2% IgG-free BSA (Jackson ImmunoResearch, West Grove, PA) and 0.1% NaN3 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... They were then washed with TBST buffer X3 (10 minutes each) and incubated with HRP-coupled secondary antibody (Jackson ImmunoResearch Laboratories Inc.) for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Neuroscience 2022Quote: ... in phosphate buffer (PB) with 0.2% Triton (PBT) was used and labelled with Cy2 via Streptavidin (1:1000, Jackson ImmunoResearch, Ely, UK) in PB ...
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently the coverslips were washed three times in wash buffer (0.05% Tween-20 in PBS) for 5 minutes and blocked for 20 minutes in 200 ml wash buffer plus 5% normal donkey serum (Jackson ImmunoResearch Laboratories). The primary antibodies were diluted in 5% normal donkey serum in PBS and incubated with the tissues 1 hour at room temperature followed by three washes in wash buffer for 5 minutes each ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then simultaneously permeabilised and blocked for 30 min at room temperature with a buffer containing 10% donkey serum (Jackson ImmunoResearch, UK) and 0.1% Triton X-100 (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed 3 times with 1X TBS-T buffer and incubated with anti-mouse (#AB_2340770) or anti rabbit (#AB_10015282) HRP secondary antibodies (Jackson ImmunoResearch Laboratories, PA, USA) for 1 hour at RT ...
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bioRxiv - Genomics 2023Quote: ... Cells were permeabilized for 5min with PBT (PBS with 0.5% Triton) and then incubated with blocking buffer (10% normal donkey serum (Jackson ImmunoResearch, Cat# AB2337258) in PBT ...
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bioRxiv - Neuroscience 2022Quote: ... (5) the slices were incubated in the solution of secondary antibodies: CyTM2 AffiniPure donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch, Ely, UK catalog number 711-225-152) and CyTM5 AffiniPure donkey anti-chicken IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... beads were resuspended in 400 μL AB per sample and 50 μL of rabbit anti-mouse bridging antibody (Jackson ImmunoResearch, Cat#315005008) was added ...
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bioRxiv - Microbiology 2021Quote: ... Samples were washed with 1 x PBST and labeled with a Cy5 AffiniPure Goat Anti-Mouse secondary antibody (Jackson ImmunoResearch Laboratories, Inc) stored in 50% glycerol (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µl of AffiniPure goat anti-human IgG Fcγ fragment specific antibody (for IgG samples, Jackson ImmunoResearch Laboratories Inc, 109-005-098) in media was added to yield a 1:1 molar ratio with each antibody dilution ...
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bioRxiv - Immunology 2023Quote: ... Samples were then incubated for 2 h with AlexaFluor 647 Goat anti-Rabbit (1:300, Jackson Immunoresearch catalog no. 111-605-144) diluted in 1x PHEM with 0.1% v/v Tween 20 ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were permeabilized and blocked in a single step with 0.2% Triton-X and 5% donkey serum (Jackson Immunoresearch, 017-000-121). Primary antibodies were subsequently added and incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed and incubated with the corresponding secondary antibodies donkey anti-rabbit AF488 and donkey anti-mouse Cy3 (Jackson ImmunoResearch Laboratories) at 1:500 in blocking solution for 1h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were incubated with Cy3 AffiniPure Donkey Anti-Mouse IgG (H+L) (1:200, Jackson ImmunoResearch, PA, USA;715-605-150) or Cy3 AffiniPure Goat Anti-Rabbit IgG (H+L ...
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bioRxiv - Biophysics 2024Quote: ... Nonspecific cross-reactivity was blocked by incubating the samples for 4 h in 1% BSA/PBS (Jackson ImmunoResearch, West Grove, PA, USA). The cells were then incubated with primary antibodies anti-RyR (IgG1 ...
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bioRxiv - Molecular Biology 2020Quote: ... After extensive washing with TBST buffer the membranes were incubated with horseradish peroxidase-conjugated goat anti-mouse secondary antibody (Jackson Immunoresearch Laboratories Inc.) at 1:10,000 dilution in TBST buffer with 5% nonfat milk for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 3 times 10 min in 0.1% Triton X-100 in PBS at room temperature and then incubated for 2h with secondary antibodies diluted in blocking buffer: donkey anti-rabbit-IgG-Cy3 antibody (1:1000, Jackson ImmunoResearch, RRID:, AB_2307443), donkey anti-mouse-IgG-Cy5 antibody (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were washed 3x with 1 mL of FACS buffer before 30 minutes of staining in FACS buffer with a 1:1000 dilution of anti-mouse IgM Alexaflour-488 secondary antibody (Jackson Immunoresearch, Cat#715545140) on ice ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was washed 3 times for 5 min each with 1X PBS + 0.2% Triton-X and then incubated overnight in blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 1:500). Upon completion of incubation with the secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 M glycine, 0.1% Triton-X, syringe filtered) for 10 min, then blocking buffer (1X PBS, 5% goat serum (Jackson ImmunoResearch; 005-000-121), 2% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated in the secondary antibody mixture: 1:30 diluted 12 nm gold conjugated donkey anti-chicken (Jackson Immunoresearch, 703-205-155), and 1:30 diluted 6 nm gold conjugated donkey anti-rabbit (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed with PBS-Tx0.1% and incubated with secondary antibodies Donkey anti-Chicken Alexa 647 (Jackson ImmunoResearch, 703-605-155, 1:500) and Donkey anti-Rabbit biotinylated (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2020Quote: ... Samples were washed three times in PBS and incubated with Alexa Fluor 488 Donkey anti-rat IgG antibodies (Jackson Immunoresearch, 1:200 dilution) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated with species-specific secondary antibodies directly conjugated to a fluorophore (donkey anti-Mouse Alexa-647 (Jackson ImmunoResearch, 715-605-150); goat anti-rabbit Alexa-594 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated with secondary antibody (PTwH, 3% donkey serum, Cy3-conjugated Donkey Anti-Goat, Jackson ImmunoResearch Labs 705-165-147, 1:1000) overnight ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed once with 200µl of FACS buffer and stained with of PE-conjugated AffiniPure Fab fragment goat anti-human IgG (Jackson Immunoresearch Cat# 109-117-008) at a 1:100 dilution and live/dead green fluorescent reactive dye (Thermo Fisher Cat# L34970 ...
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bioRxiv - Bioengineering 2021Quote: ... embryos were washed 3 times (30 min intervals between washes) in the staining buffer and labeled using donkey-anti-rabbit-biotin secondary antibody (Jackson ImmunoResearch, 711-065-152) in the staining buffer at 4°C overnight ...
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bioRxiv - Bioengineering 2021Quote: ... the embryos were washed 3 times in the staining buffer (30 mins intervals between washes) and labeled with Alexa Fluor 488 Streptavidin (Jackson ImmunoResearch, 016-540-084). Labeled embryos were washed 3 times in the staining buffer (30 minutes between washes ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X with TBS containing 0.1% Tween-20 and incubated for 1 hr RT with secondary antibody conjugated to horseradish peroxidase diluted into blocking buffer (Jackson ImmunoResearch—West Grove, PA). Blots were washed a final 3X and imaged using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... the digestion buffer was removed and the gels were incubated with streptavidin Alexa Fluor 647 (1:100; Jackson ImmunoResearch 016–600-084, lot: 124695) in PBS for 2h at RT ...
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bioRxiv - Physiology 2021Quote: ... The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500, #115-545-205, Jackson Immunoresearch, West Grove, PA) for 1 hour at room temp ...
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bioRxiv - Biophysics 2020Quote: ... blots were washed in 1X TBS-T buffer and incubated with respective horseradish peroxidase (HRP) conjugated secondary Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 715-035-150) or Anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 hour at room temperature (RT) in a blocking buffer containing 10% normal donkey serum (NDS) (cat. 017-000-001, Jackson ImmunoResearch, West Grove, USA), 1% bovine serum albumin (BSA ...