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Citations for Beckman :
351 - 379 of 379 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... CLP assembly reactions were incubated at 25°C for 30 min before loading onto 15-30% (wt/wt) sucrose gradients and spinning at 159,599 X g for 43 min at 4°C (Beckman Coulter TLS 55 rotor). Gradients were immediately fractionated and aliquots of each fraction were denatured by boiling in 1% SDS to ensure antibody access ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Biochemistry 2021Quote: ... supernatants were collected and centrifuged with 150.000 x g (at rav) for 30 min at 4 °C using an Optima™ MAX-XP Ultracentrifuge (Beckman Coulter; TLA120.2 rotor). Pellets were resuspended in PBS and 4 µg of primary antibody were added for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting lysates were centrifuged at 100,000Xg for 30-60 min at 4°C in an Optima TLX tabletop ultracentrifuge using a TLA-110 rotor (Beckman Coulter, Fullerton, CA). The clarified supernatants were removed and transferred into the new tubes ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Systems Biology 2020Quote: ... insoluble material was pelleted down at 2500 RCF for 10 minutes at 4C and the collected supernatant was subjected to ultracentrifugation (35000 RPM for 30 min. at 4C, Beckman-Coulter Optima Ultracentrifuge) to remove bacteria ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 10,000xg for 30 minutes at 4°C in a Beckman Coulter Optima XE-100 (fitted with a SW41Ti Rotor (Beckman Coulter, CA USA)) ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Neuroscience 2021Quote: ... 30s at 60°C and 60s at 68°C) and DNA was cleaned and purified using magnetic SPRI beads (Beckman Coulter, Ca# B23317). Libraries were resuspended in 25μl Low EDTA TE buffer and concentration was measured using a Qubit dsDNA HS Assay Kit ...
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bioRxiv - Microbiology 2022Quote: Whole-cell absorbance spectra from 550 to 800 nm were taken from cells grown in BG-11 under VL and FRL for 30 days using a DU 640 spectrophotometer and associated software (Beckman Coulter, Brea, CA). For low-temperature fluorescence measurements at 77 K ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 Tl rotor (Beckman ...
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bioRxiv - Microbiology 2021Quote: ... Ten milliliters of allantoic or amniotic fluid was centrifuged at maximum speed (16,000 × g) for 30 min at 4 °C using a floor ultra centrifuge (Beckman Optima L-60 Ultracentrifuge). After removal of the supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting lysate was loaded on top of 10 ml 29% iso-osmolar OptiPrep solution in a conical 30 ml centrifuge tube (Beckman Coulter, SW28 rotor). Samples were centrifuged at 10,100 × g (7,500 r.p.m. ...
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bioRxiv - Zoology 2023Quote: ... Colour development was stopped after 30 minutes with 2M H2SO4 and absorbance determined at 450 nm in a DTX880 multimode detector (Beckman Coulter, MN, USA) using the SoftMax® Pro program (Version 7.1.0 ...
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bioRxiv - Neuroscience 2022Quote: ... Five samples were processed in parallel and each homogenate was transferred to a separate 30 mL thick wall polycarbonate ultracentrifuge tube (Beckman Coulter, Cat # 355631). Sucrose solution (1.8 M sucrose ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and pelleted through a 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Microbiology 2022Quote: ... The clarified supernatant was collected and pelleted through a 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Cell Biology 2023Quote: ... the resulting G-actin mix was ultracentrifuged at 90K rpm for 30 minutes at 4°C in an ultracentrifuge (Beckman, Optima TL 100) to remove actin oligomers and/or aggregates.
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bioRxiv - Microbiology 2024Quote: ... The clarified supernatants were collected and pelleted through 30% (w/v) sucrose cushions at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 h at 10°C ...
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bioRxiv - Neuroscience 2024Quote: ... cells were harvested and lysed in the lysis buffer described above and ultracentrifuged at 100,000 x g for 30 min at 4 °C using the TLA-110 rotor and the Optima™ MAX-XP tabletop ultracentrifuge (Beckman, Brea, CA). The supernatants were collected as “triton-soluble fraction” and the pellets were washed with the lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... an ultracentrifuge capable of spinning 50 ml falcon tubes at 10,000 rpm (Beckman Coulter Avanti J-30 I ultracentrifuge and a Beckman JA-12 fixed angle rotor), falcon tubes (polypropylene ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1% DMSO) by pin tool to 30 µL of the reaction mixture A (buffer assay mixture and substrate) with shaking (Beckman Biomek FXP, Brea, CA). The activity assay was started by adding reaction mixture B (enzyme in storage buffer ...
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bioRxiv - Genomics 2024Quote: ... After the End-prep reaction the DNA was purified with 30 μl of AMPure XP beads according to manufactures manual (Beckman Coulter Genomic, CA, USA) and resuspended in 11 μl of nuclease-free water.
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bioRxiv - Immunology 2023Quote: ... Suspended cells were incubated in the dark for 30 min at room temperature with CD66b-APC-Alexa Fluor 750 (Beckman Coulter Life Sciences, B08756) for the detection of neutrophils ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mg of nuclear extract was deposited over a 14-mL 10-30% glycerol gradient prepared with HK buffer in a 14× 95-mm centrifuge tube (Beckman Coulter, Brea, CA Cat. # 344060) by using the Biocomp Gradient Station (Fredericton ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was transferred to a polycarbonate ultracentrifuge tube and centrifuged at 350 kg for 30 min (TL-100; Beckman Coulter, TLA-100 rotor (20 × 0.2 mL) to isolate the cytosolic fraction ...