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Citations for Beckman :
251 - 300 of 379 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the post-nuclear supernatant was centrifuged for 30 min at 100,000 x g at 4°C using a S120AT2 rotor (Beckman Coulter). The resulting pellet containing the membranes was resuspended in SDS sample buffer (see below).
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bioRxiv - Cell Biology 2022Quote: ... Lysates were cleared by centrifugation for 30 min at 4°C at 18,000 rpm (SS-34 rotor; Beckman-Coulter, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Centrifugations were performed under the various conditions indicated in the REULTS section using JXN-30 with a JS-24.38 rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... clarified by centrifugation at 20,000 rpm (48,385 x g) for 30 min in a JA25.50 rotor at 4 °C (Beckman AvantiTM J-25), filtered through a 0.8/0.2 AcroPak Filter Unit (Pall) ...
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bioRxiv - Immunology 2023Quote: ... This clear media supernatant was loaded on top of 30% sucrose gradient and was followed by ultracentrifugation at 100,000xg for 90mins on SW41Ti (Beckman Coulter) rotor ...
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bioRxiv - Immunology 2023Quote: ... virus solutions were clarified of debris by centrifugation at 10,000 rpm at 4°C for 30 minutes in a chilled SW32Ti rotor (Beckman Coulter), followed by pelleting through a 30% cold sucrose solution at 25,000 rpm for 90 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted extract was then centrifuged at 31,200 rpm for 30 minutes at 4°C in a Type 70 Ti rotor (Beckman Coulter). The supernatant was filtered through a 0.45 µm syringe filter (Millex-GP PES membrane (Millipore SLHP033RS ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were harvested at 6000 rpm for 30 min in a JLA 9.1000 rotor (Beckman Coulter, Brea, California, USA), washed with 150 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was clarified via centrifugation at 30,000 rpm for 30 min in a Type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... and centrifuged at 10,000 x g for 30 min at 4°C using a TLA100.3 fixed angle rotor and an Optima TLX Ultracentrifuge (Beckman Coulter).
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bioRxiv - Neuroscience 2024Quote: ... Full-length double-stranded cDNA was purified with 30 μL (0.6x) of AMPure XP magnetic beads (Beckman Coulter, Cat.#A63881) and eluted in 20 μL of water ...
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bioRxiv - Microbiology 2024Quote: ... by staining with Hoechst 33342 for 30 min at 37°C and then analyzed by flow cytometry (Beckman Coulter Cytoflex) to determine parasitemia using the 405 nm laser ...
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bioRxiv - Neuroscience 2024Quote: ... The precleared media was then layered dropwise onto a 30% (w/v) sucrose cushion in an ultracentrifuge tube (Beckman Coulter). Ultracentrifugation was done at 134,000xg for 4h at 4°C using an SW 41 Ti rotor (Beckman Coulter ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge; Beckman Coulter). The supernatant was discarded ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 minutes at 37°C to remove DNA contaminants and bead-purified using Agencourt AMPure XP (A63881, Beckman Coulter). Sequencing libraries were prepared using CORALL Total RNA-Seq with RiboCop rRNA depletion (Lexogen ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... Cultures of 1 ml in PYNal were incubated at 30°C (250 rpm) to an optical density of 0.2 at 620 nm (OD620; Beckman DU650 spectrophotometer) in 96-deep-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions were diluted with 2.5 ml sterile PBS and centrifuged for 30 min at 100,000 × g (43,000 rpm) in a TLA 110 rotor (Beckman, Optima TL100 centrifuge). These concentrated fractions were resuspended in 30 μl sterile PBS prior to further assessment by electron microscopy or RNA preparation for sequencing.
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We performed daily 1:210 dilutions of populations in YPD 30°C and SC 30°C and daily 1:28 dilutions of populations in SC 37°C using a BiomekFXp robot (Beckman Coulter). Before dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... 30 minutes at 10,000 g and 70 minutes at 100,000 g (using a Beckman XL-70 centrifuge with a SW28 rotor). EVs pellets were washed in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter, Brea, CA) and the pellet was resuspended in BRB80 supplemented with 10 µM taxol (BRB80T).
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bioRxiv - Immunology 2021Quote: ... were incubated with monoclonal antibodies at 4°C for 30 min and then fixed with 4% paraformaldehyde before analysis on a Cytoflex cytometer (Beckman Coulter). Data were analyzed by Flow Jo software v.10.7 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was overlaid with decreasing concentrations of Nycodenz (30%, 20%, 10% and 0%) and centrifuged in a SW55 rotor (Beckman Coulter) 2h 30 min at 55000 rpm and 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% Paraformaldehyde for 30 minutes at room temperature and analyzed for GFP fluorescence on a Gallios flow cytometer (Beckman-Coulter). For SARS-CoV-2 experiments ...
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bioRxiv - Cell Biology 2020Quote: ... The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter). The second PCR was performed for 15 cycles for wild type-specific and for 21 cycles for tag-specific amplicons respectively using 60°C annealing and 30 s elongation ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysate was sonicated on ice and clarified by centrifugation for 30 min at 4°C and 35,000 rpm in a Beckman L90K Optima and 50.2 Ti rotor (Beckman, Brea, CA, USA). The clarified lysate was applied to a HisTrapHP column (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: 1 mg of nuclear extract supplemented with protease inhibitors was carefully overlaid onto a 10 ml 10-30% glycerol gradient prepared in a polyallomer centrifuge tube (Beckman Coulter). Tubes were placed in a SW-41 swing bucket rotor and centrifuged at 4°C for 16 hours at 40,000 rpm ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were resuspended in 200 μl and stained on ice 30 minutes with APC conjugated anti-CD56 antibody (Beckman Coulter) and subsequently washed ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 4°C at 31,000 rpm for 2 h and 30 min using an SW 32 Ti rotor(Beckman Coulter, 369694). The gradients were fractionated using a Teledyne ISCO Density Gradient Fractionation System and 12 fractions were collected ...
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bioRxiv - Microbiology 2021Quote: ... The remaining lysate was layered on top of a 30% (w/w) sucrose cushion and centrifuged using a Beckman SW41 rotor (Beckman Coulter) at 32,000rpm and 4°C for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... PacBio libraries were constructed using the manufacturer’s protocol and were size selected for 30 kb fragments on the BluePippin system (Sage Science) followed by subsequent purification using AMPure XP beads (Beckman Coulter). The PacBio libraries were sequenced on a PacBio RSII system with P6C4 chemistry ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate was incubated under rotation for 30 min at 4 °C and cleared for 10 min in an MLA-80 rotor (Beckman Coulter) at 11,500 xg and 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... The assembled complexes were incubated for 5 min at 30 °C and for 10 min on ice before they were loaded on a 30% (w/v) sucrose cushion in TLA-100 tubes (Beckman Coulter). The sucrose cushions were centrifuged for 2 h at 390,880 xg and 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The clarified lysate was layered onto a 1 ml cushion of 30% iodixanol (OptiPrep) in Buffer A in clear ultracentrifuge tubes (3.5 ml thickwall polycarbonate tubes, Beckman Coulter #349622). Ultracentrifugation was run for 3 hours at 100,000 g ...
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bioRxiv - Cell Biology 2020Quote: ... The polymerized microtubules were centrifuged for 30 min at 18.000 x g in a Microfuge 18 Centrifuge (Beckman Coulter, Brea, CA) and the pellet was resuspended in BRB80 supplemented with 10 µM taxol (BRB80T).
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bioRxiv - Cell Biology 2020Quote: ... 50 µl of alkaline carbonate treated mitochondria were set aside as input (kept on ice) and 50 µl were centrifuged at 100 000g for 30 min at 4°C in TLA100 rotor (Beckman Coulter). Pellet was gently washed with ice-cold sodium carbonate buffer and resuspended in 20 µl of 1x Laemmli loading buffer ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... the 4 tubes were centrifuged using JA-20 rotor at 43,000 × g for 1 hour and 30 minutes in Beckman coulter® Avanti j-26 xp (Beckman, France). The pellets of the 4 tubes were preserved at −80°C before transport and metagenome sequencing and analysis.
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bioRxiv - Molecular Biology 2021Quote: ... The samples were then separated into membrane and matrix fractions by ultracentrifugation at 100 000 g (TLA 110 rotor) for 30 min at 4°C in OptimaTM TLX table-top ultracentrifuge (Beckman-Coulter). The supernatant fraction was transferred to new tube ...
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bioRxiv - Immunology 2021Quote: ... 6 mL of blood was layered on top and samples were centrifuged at 930 x g for 30 minutes at room temperature (RT) with no deceleration in an Allegra X-12R centrifuge (Beckman Coulter). The neutrophil layer was collected between the Histopaque layers and diluted in 4°C PMN buffer (0.5% BSA ...
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bioRxiv - Immunology 2021Quote: ... Insoluble proteins and cell debris were removed by centrifugation (13,000 rpm, 30 minutes at 5°C, JA-30.50Ti rotor, Beckman, Palo Alto, CA). The soluble CuMVTT CP protein in clarified lysate was pelleted using saturated ammonium sulfate (1:1 ...
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bioRxiv - Genomics 2022Quote: ... samples were then transferred to a new 96-well plate at a standard volume (typically 20-30 μL) using a BioMek FXp liquid handler (Beckman Coulter). This plate was diluted to a final TIME-Seq starting concentration of 10 ng/μL using the Mantis liquid handler.
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bioRxiv - Biochemistry 2022Quote: ... Soluble and insoluble fractions were isolated via centrifugation (90,000 x g, 30 min, 4 °C) in an Optima™ MAX-OP (Beckman Coulter) centrifuge using the TLA-100.3 rotor ...
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bioRxiv - Physiology 2022Quote: ... extracted myosin samples were precipitated using ten times the volume of ultrapure water containing 2 mM DTT and 0.5 mM AEBSF.After incubation for 30 min on ice and centrifugation at 40000 rpm (Beckman Coulter, TLA 120.2), the pellet was resuspended in 1D sample buffer (62.5 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... The virions were harvested from the supernatant by centrifugation through 1.5ml of 30% sucrose at 10,000 RPM at 4°C for 2hrs (Beckman L8-70M Ultracentrifuge). The resulting pellet of virions was resuspended in 20ul of RIPA lysis buffer containing protease inhibitors.
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bioRxiv - Cell Biology 2024Quote: ... The polymerized microtubules were diluted in BRB80T and centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation the pellet was resuspended and kept in BRB80T at room temperature.
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Caspase cleavage of Influenza A virus M2 disrupts M2-LC3 interaction and regulates virion productionbioRxiv - Microbiology 2024Quote: ... Resuspended pellets were layered on top of a sucrose density gradient (30-60 %) prepared in thinwall ultracentrifuge tubes (Beckman Coulter, 331372), which were placed in SW41 rotor buckets ...
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bioRxiv - Cell Biology 2024Quote: ... Microtubules and tau were incubated for 10 min at room temp and centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter). After centrifugation the supernatant was separated from the pellet and used as the sample indicated by the ‘tau in solution‘ ...
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.